1.Long term effect of calcium carbonate bone cement combined with bFGF in repairing pulp chamber perforation
International Journal of Biomedical Engineering 2017;40(3):169-172
Objective To investigate the long-term efficacy of calcium phosphate cement combined with bFGF in repairing pulp chamber perforation and to analyze the correlation between the diameter of the perforation and the curative effect.Methods 75 patients with pulp chamber perforation (82 teeth) were enrolled and divided into the observation group and the control group according to the repair material and method.A series of subgroups were also modeled according to the diameter of the perforation,which include the control group A (≤ 1.5 mm),control group B (1.6~3 mm) and control group C (>3 mm),as well as the observation group A (≤ 1.5 mm),observation group B (1.6~3 rmm) and observation group C (>3 mm).The observation group was treated with calcium phosphate cement combined with bFGF,and the control group was treated with calcium phosphate cement alone.Results The total effective rate of the observation group was 97.8%,which was significant higher than 80.6% in the control group (P<0.05).The cure rate of the observation group A was 100%,which was significant higher than 73.3% in the observation group B and 41.7% in the observation group C (all P<0.05).The total effective rates of the observation group A and B were significantly higher than 91.7% in the observation group C (all P<0.05).The cure rate of the control group A was 92.9%,which was significant higher than 60.0% in the control group B and 25.0% in the control group C (all P<0.05).The total effective rates of the control group A (100%) and B (90.0%) were significantly higher than 91.7% in the control group C (all P<0.05).Conclusions Calcium phosphate cement combined with bFGF in repairing the pulp chamber perforation was significantly better than calcium phosphate cement alone.The cure rate of perforation repairing is closely related to the perforation size.The perforation with small diameter may achieve a better repairing effect.
2.miR-34c suppresses cell proliferation and invasion of prostate cancer PC3 cells
Hao LIU ; Jiang YIN ; Zhimin HE
Journal of Chinese Physician 2014;16(9):1168-1171
Objective To explore the effect and molecular mechanism of miR-34c in cell proliferation and invasion of human prostate cancer PC3 cells.Methods miR-34c was synthesized and transfected into prostate cancer PC3 cells.The ability of cell pro liferation was examined with methyl thiazolyl tetrazolium (MTT),and invasion with Transwell assay.The level of c-met mRNA was analyzed by real-time reversing transcription polymerase chain reaction (RT-PCR).Western blot was used to detect the expressions of cmet,cyclin D1,and EMT-associated markers.Results Over-expression of miR-34c reduced PC3 cell proliferation,and inhibited the abilities of invasion.Bioinformatics analysis showed that 3'-UTR of c-met had two miR-34c matched sites,and miR-34c inhibited the expressions of c-met mRNA and protein.Meantime,miR-34c obviously decreased the expressions of cyclin D1 and N-cadherin,but increased the expression of E-cadherin in PC3 cells.Conclusions miR-34c may suppress cell proliferation and invasion in prostate cancer PC3 cells,by which mechanism is related to reduction of the expressions of c-met,cyclin D1,N-cadherin,and upregulation of E-cadherin.
3.Determination of Mercury in Blood by Atomic Fluorescence Spectrometry with a Thiourea Extraction Method
Zhimin SONG ; Anshan XIAO ; Suxia JIANG
Journal of Environment and Health 2007;0(07):-
Objective To develop a method for determination of mercury in blood by atomic fluorescence spectrometry. Methods Using a thiourea extraction method, the mercury in blood is determined by atomic fluorescence spectrometry. Results The linearity of calibration curve of mercury was in the concentration of 0.000-10.00 ?g/L. The detection limit was 0.06 ?g/L. The recovery rates were 95.5%-100.3%.The RSDs were 4.1%-4.5%. Conclusion The method has the advantages of simple operation, high sensitivity, good repeatability and is applicable to the determination of mercury in blood.
4.Percutaneous treatment of lumbar disc herniation by oxygen-ozone injection-clinical study with indication
Zhuying WANG ; Caimei JIANG ; Zhimin WANG
Journal of Interventional Radiology 1994;0(02):-
Objective To study the clinical effect and the range of indications of oxygen-ozone treatment for lumber disc herniation. Methods 6~15 ml of oxygen-ozone(35~45 ?g/ml) were injected percutaneously into lumbar disc.In case of multiple disc herniations,the proceduce could be taken with two discs for once. Results 323 patients with 433 discs were treated by oxygen-ozone injection procedure.Total effective rate was 77.7%. Conclusions The treatment of lumber disc herniation by oxygen-ozone injection is simple,safe and effective with mild trauma.Oxygen-ozone not only can oxidize the proteoglycan in the nucleus leading to the contraction of nucleus,but also provide anti-inflammation effect with pain relief and without complication yet.
5.Monitoring of Adverse Drug Reactions in Secondary or Inferior to Secondary Medical Institutions
Dongmei YANG ; Zhimin ZHANG ; Lei JIANG
China Pharmacy 2005;0(22):-
OBJECTIVE:To provide reference for the practice of monitoring on adverse drug reactions (ADRs) in secondary or inferior to secondary medical institutions.METHODS:The problems in the practice of ADR monitoring in secondary or inferior to secondary medical institutions were analyzed and the approaches to strengthen the ADR monitoring was discussed taking our hospital as an example.RESULTS & CONCLUSIONS:The problems in the practice of ADR monitoring in secondary or inferior to secondary medical institutions manifested as low general technical level,lacking of advanced information network,hospital leaders' negligence,medical staff's poor consciousness,pharmacy departments' failure in bringing their role into full play,etc.Therefore,it is urgent to attach great importance to the problems to facilitate the development of ADR monitoring by enlarging publicizing,raising awareness;improving the organizational structure and the related working rules,bringing clinical pharmacists and nursing staff's roles into full play and establishing the reward and punishment mechanism,etc.
6.Clinical Study of the Collagenase Injection Combined Oxygen-Ozone for the Treatment of Lumbar Disc Herniation
Zhiqun WU ; Zhimin WANG ; Caimei JIANG
Journal of Practical Radiology 2001;0(05):-
Objective To assess the therapeutic effect of oxygen-ozone combined collagenase injection for the treatment of lumbar disc herniation.Methods Oxygen-ozone and collagenase were injected into the lumbar disc or outside the lumbar disc to treat the lumbar disc herniation.After the treatment,the patients were followed-up for 6~12 months,then the results were assessed according to the Macnab criteria.Results Of 183 patients,the effect was excellent in 144,good in 18,fair in 16 and poor in 5.The curative rate was 88.5%,the effective rate was 97.3%.No serious complications occurred in this group.Conclusion Oxygen-ozone combined collagenase injection is very effective,safe,and cheap method for treating lumbar disc herniation.
7.Analysis of 217 death of road traffic accident case about electric bicycle
Chinese Journal of Forensic Medicine 2017;32(z1):1-3
Objective Explore the characteristics of road traffic fatalities about electric bicycle.Methods Through collecting 217 road traffic accident cases in our city,according to gender,age,transportation ways,incident section,cause of death and such as classification statistical analysis.Results In the 217 road traffic accident cases the dead men are more than women(1.6 ∶ 1.0),73.7% of the dead between 21 and 60 years old;the vehicles causing accident mainly were cars and trucks,31.89% and 32.3% respectively;53.5% of the accident at the intersection,and the 217 road traffic accident cases on suburbs and towns more than urban area(73.3∶26.7);traffic accident high-risk period of the day is 6:00 ~8:00,17:00 ~19:00,23.0% and 24.4% respectively;75.6% of the death is brain injury,31.8% and 30.0% death happened on the spot and rescue invalid death in the day of the road traffic accident.Conclusion We need targeted prevention based on the characteristics of road traffic fatalities about electric bicycle.
8.Efficacy of itopride in functional dyspepsia patients overlapping constipation-predominant irritable bowel syndrome
Huimin CHEN ; Xiaobo LI ; Zhimin JIANG ; Yunjia ZHAO ; Zhizheng GE
Chinese Journal of Digestion 2010;30(2):102-105
Objective To assess the efficacy of itopride on treatment of patients with functional dyspepsia (FD) overlapping constipation-predominant irritable bowel syndrome (C-IBS). Methods Patients who met criteria for FD and FD overlapping C-IBS were randomly assigned into FD treatment group (group A), FD control group (group B), FD overlapping C-IBS treatment group (group C) and FD overlapping C-IBS control group (group D). The patients in group A and group C received 100 mg of itopride 3 times daily for 8 weeks. Dyspeptic symptoms including abdominal pain, bloating, early satiety and constipation, were evaluated before and after treatment. Ultrasonic monitoring of gastric emptying function was performed in group A and group C before and two weeks after treatment.ResultsThe symptoms of FD were relieved in both group A and group C (P<0.05), while better results were shown in group C. The significant improvement of constipation was seen in group A and group C. Besides, after medication, gastric emptying was improved in group A and group C in comparison with group B and group D. Conclusion Itopride is an effective therapeutic option in the treatmentping of patients with overlapping of FD and C-IBS.
9.Investigation on low doses of metformin induce hepatoma cells senescence and its underlying mechanism
Jifang LIU ; Min DENG ; Jiang YIN ; Zhimin HE
Chinese Journal of Primary Medicine and Pharmacy 2014;21(13):1921-1924
Objective To explore the effect of metformin on hepatoma cells senescence and the underlying mechanism.Methods Cell proliferation,cycle and apoptosis were examined by MTS and flow cytometry assay in response to different concentrations of metformin(0,0.01,0.1,1,10 and 50mmol/L).Senescence-associated β-galactosidase (SA-β-ga1) staining and senescence marker Dec1 protein levels were used to evaluate the effect of metformin on hepatoma cells senescence.In addition,protein expression of p-AMPK,p-ACC and AMPK was detected by Western blot analysis.Results Metformin suppressed proliferation of HepG2 cells in a dose-dependent manner.High concentrations of metformin (10 and 50mmol/L) promoted cell apoptosis,while lower doses of metformin (0.01,0.1 and 1mmol/L) led to enlarged and flatten senescent morphology and increased SA-β-ga1 positive cells.Moreover,cell cycle was blocked in G0/G1 phase and protein levels of senescent marker Dec1,p-AMPK and p-ACC were significantly enhanced,whereas AMPK protein expression was almost unchanged.Conclusion We showed here that high dose of metformin promotes HepG2 cells apoptosis,but low doses of metformin induce cellular senescence,which may be related to the activation of AMPK signaling.These data will provide vital evidence for improving the outcome of comprehensive treatment in HCC patients by driving hepatoma cells to undergo senescence.
10.Curcumin induces U251 cell apoptosis via alteration of expression of FAK and activation of caspase
Zhimin WU ; Xianhou YUAN ; Pucha JIANG ; Zhiqiang LI ; Tao WU
Chinese Pharmacological Bulletin 1986;0(04):-
Aim To study the mechanism of curcumin induced U251 cell apoptosis. Methods U251 cells were treated with 20~100?mol?L~ -1 curcumin for 24 h and the growth inhibition rates of U251 cells were measured with MTT method. MTT method was used to measure the caspase inhibitors effect on curcumin too. Cell apoptosis was inspected with flow cytometry (FCM) and observed using electron microscope. The protein levels of FAK in U251 cells were observed by SP immunocytochemistry. The activity of caspase-3 was measured by spectrofluorometry. Results Curcumin inhibited the proliferation of U251 cells,induced apoptosis of U251 cells. At the same time the protein levels of FAK in U251 cells decreased and the activity of caspase-3 increased significantly. The apoptosis of U251 cells was partially reversed by caspase inhibitors. Conclusion Curcumin induced apoptosis via inhibition of expression of FAK and activation of caspase-3.