Objective To study the HPLC fingerprints (HPLC-FPS) Taxus cuspidatea twigs and leaves (TCTL) for reflecting the internl chemical information and evaluating its internal quality of TCTL. Methods HPLC was used to analyze the alcohol extracts of TCTL. Results The fingerprint of TCTL was established. HPLC-FPS of ten batches of T. cuspidata samples collected at different time were analysed and extablished. The correlation technicl parameters of T. cuspidata were obtained, i.e. the main common peak with relative retention time and relative peak area. Conclusion The fingerprint can be used to evaluate the quality of TCTL.

Objective To investigate the possibility of blood flow resistance index (RI),pulse index (PI) plus the risk of malignant index (RMI) in preoperative diagnosis of ovarian neoplasm.Methods Four hundred and sixty-eight women with ovarian tumor,30 years or older,were enrolled in current study from January 2007 to December 2012 in the People's Hospital of Tianjin.Among them,127 were with malignant ovarian tumors and 341 with benign ovarian tumors.They were retrospectively prospective assessed by RI + PI,RMI,and RI + PI combine with RMI.Results The value of RI,PI of malignant ovarian tumors group were lower than those of benign ovarian tumors group ((0.41 ± 0.08) vs (0.68 ± 0.15),t =28.83,P ＜ 0.01 ; (0.82 ± 0.24) vs (1.67 ±0.71),t =22.26,P ＜0.01).RMI of malignant ovarian tumors group was (269.7 ±41.2),higher than that of benign ovarian tumors group(25.6 ± 11.4,t =107.55,P ＜0.01).The indices of RI ＜0.40 and/or PI ＜1.00,RMI ＞ 200 were served as the critical biomarker respectively.Histopathological examination result was conducted and considered as gold standard for identifying patients with ovarian cancer pelvic masses.The sensitivity of RI,PI plus RMI was 92.57％,higher than that used by RI and PI (81.02％) or RMI only (84.21％),and the difference was significant (P ＜ 0.05).The sensitivity,specificity,positive and negative predictive values of the combined index of RI,PI adding to RMI were all higher than 90％.Conclusion The combined index of RI,PI plus RMI was proved to enhance the diagnostic accuracy regarding of the preoperative diagnosis of benign or malignant ovarian tumors.

The authors discuss the technical problems in clinical evaluation of newly-developed Chinese medicines.For improvement of evaluation quality,this paper puts forward some suggestions for solving the common problems such as unitary intervention pattern,lack of corresponding treatment for the changed syndrome,subjectivity in standards of syndrome-differentiated diagnosis,ill-chosen medicine in control group,and mismanagement of medicine combination.

Objective\ To evaluate the importance of coronary arteriography with acute coronary syndromes (ACS).Methods\ The clinical data of 271 ACS patients with coronary arteriography and electrocardiogram were compared.Results\ The incidence of normal coronary arteriography with ACS patients occurred in 21 03%,220 of 271 patients had ischemic ST-T change or abnornal Q wave (81 1%).The change of anterior wall was system frequently accompanied by LAD or three branch lesions,inferior wall and inferior wall system frequently accompanied by RCA,Lcx and RCA or three branch lesions,anterior wall and inferior wall system freguently by LAD and RCA or three branch lesions,respectively.The lesions were more than one branch and severe in patients with unstable angina.Q-wave myocardal infarction usually occurred in single branch and severe lesione,non-Q wave myocardial infarction usually occurred in other branches and severe lesion.Conclusion\ Patients with ACS should be performed coronary arteriography,it is very significant in correct diagnosis of coronary heart disease,fixed position and severe degree of lesion vessel.

Objective:To investigate the feasibility of improving the corrosion resistance of nickel-chromium alloys by the means of cryogenic treatment. Methods:16 disk samples were divided into 4 groups, including control group(C), cryotreat group(E1),tempering after cryotreat group(E2) and simulative porcelain sintering after cryotreat group(E3). All samples were subjected to electrochemical potentiodynamic polarization after immersed in the artificial saliva ISO/TR 10271:1993(E) pH 6.8 for 24 h at ambient temperature. The metallography of all groups were observed by SEM. Results:①Cryogenic treatment elevated the pitting corrosion potential and dropped the corrosion current of Ni-Cr alloys. Following simulative porcelain sintering,as well as tempering after cryotreat, decreased the corrosion current. But the corrosion potential of Ni-Cr alloys fell after cryotreat and following simulative porcelain sintering. Tempering after cryotreat did not affect the pitting corrosion potential. ②Much small secondary substance separated out and evenly distributed along the crystals of Ni-Cr alloys after cryotreat. Conclusion:Cryogenic treatment effectively improves the electrochemical corrosion resistance of Ni-Cr alloys, and tempering following cryotreat is unnecessary. It is not recommended to sintering porcelain after cryogenic treatment.

Aim To establish the technology of isolation rat’s mesenteri c lymphatics and the culture method of their endothelium and confirm the ability o f synthesis nitric oxide and Factor Ⅷ related antigens in the endothelium in vitro. Methods With an operating microscope and microsurgical in struments, rat’s mesenteric lymphatics were isolated and the endothelium were p rimarily cultured and subcultured. Exerting immunohistochemical method, Factor Ⅷ related antigens and nitric oxide synthase were evaluationed. The contents of NO metabolite were measured. Results The rat’s mesenteric lympha tics were isolated and the endothelium were Fusiform shape or polygon, the monol ayer cultures displayed typical cobblestone or pavingstone morphology. Factor Ⅷ related antigens were expressed and their positive rate was 98%. The positive rate of nitric oxide synthase was 83%. Positive reaction products were buffy ar ound nuclear. NO metabolite was (10.26?2.17) mol?L -1(n=7). Conclusion The culture method we introduced was very practical, simple and reliable. The endothelium is in normal structure and function characters. It also certificated that the endothelium in vitro could express nitric oxide synthase and Factor Ⅷ related antigens.

Objective To study the change of mid-regional pro-adrenomedullin(MR-proADM) level in patients with chronic heart failure and its significance.Methods Randomly selected 330 patients with chronic heart failure,including 120 cases of grade Ⅱ and Ⅲ,90 cases of grade Ⅳ,90 cases were selected.,Within 24 h after the entry of the group and 7 d after symptom relieved,serum N-terminal pro-B-type natriuretic peptide(NT-proBNP),MR-proADM,cardiac ultrasound(left ventricular diameter(LVEDD) and left ventricular function(LVEF)) were measured.Ninety physical examination in the same period as healthy control during the same period.The change of MR-proADM level in patients with chronic heart failure and its significance were analyze.Results Compared with the healthy control group,the level of MR-proADM in patients with chronic heart failure(grade Ⅱ:(641.55±106.34) pmol/l;grade Ⅲ:(684.46±101.33) pmol/l;grade Ⅳ:(737.81±479.37) pmol/l) was significantly higher than that in the control group(610.22±60.84) pmol/l),the difference was statistically significant(F=5.33,P=0.001).At the same time,the level of MR-proADM was increased with the aggravation of heart failure,after drug treatment will decline((608.09±100.81),(617.64±94.32),(642.22±163.53) pmol/L).And MR-proADM levels were significantly positively correlated with NT-proBNP levels and NYHA classification in patients with heart failure(r=0.194,P=0.003;r=0.206,P=0.000).Conclusion MR-proADM has important guiding significance in the diagnosis,classification of patients with chronic heart failure.

AIM: To investigate the effect of cold exposure on the thermoregulatory function in hypothyroid rats. METHODS: Hypothyroid model was established by administration of antithyroid drug propylthiouracil(PTU) in rats. Colonic temperature was measured using digital thermometer. Plasma T_3 and T_4 concentrations were determined by radioimmunoassay. The effect of cold exposure on body temperature was observed after 2 weeks of PTU treatment. RESULTS: Plasma T_3 and T_4 concentrations were reduced markedly 2 weeks after PTU treatment, and colonic temperature was also decreased markedly; Hyperthermic response was not different between PTU group and control group during cold exposure. CONCLUSION: This study suggests that PTU-induced hypothyroid rat possesses a robust thermogenic response to short bouts of cold exposure.

Objective To investigate the diagnostic value of the combination of exercise electrocardiography test(EET) and coronary artery calcification(CAC) determined by electron beam computed tomography(EBCT) in coronary artery disease.Methods One hundred and thirty-five patients underwent coronary angiography,EET and EBCT.The value of calcium score(CS) and EET in diagnosing coronary artery disease were separately and unitedly evaluated.Results According to calcification score cut points by age and gender,the sensitivity,specificity,positive and negative predictive value of CAC for diagnosing coronary artery disease were 77%,81%,74% and 83%,respectively.The corresponding parameters of EET were 73%,76%,68% and 80%.After combining CAC and EET,the sensitivity and negative predictive value of parallel test were 93% and 92%,and the specificity and positive predictive value were 96% and 91%,respectively.Conclusions Exercise electrocardiography test combined with coronary artery calcification determined by electron beam computed tomography can significantly improve their diagnostic value for coronary artery disease.

Objective To investigate the short-term effect of PTCA on contractile function of hibernating myocardium. Methods Fifty-three patients with coronary heart disease and segmental dysfunction underwent echocardiography and radionuclide imaging. Echocardiography was repeated one week after PTCA. Results (1) Recovery of contractile function occurred in 87 revascularized segments with dysfunction (87/167, 52.1%) compared with only 2 nonrevascularized segments (2/59, 3.4%) (P