The scientific research was integrated with medical statistics experiments in view of the experimental teaching practice of medical statistics.The experimental teaching system and evaluation methods of medical statistics were formed,characterized with research task-oriented,a combination of theory,practice and scientific research to guide the students to study medical statistics systematically,such as project design,collecting data,sorting data and analyzing data.The teaching method boosts the students' enthusiasm,and cultivates students' ability to solve practical problems by using statistical principles.

Acute ligation of the common carotid artery on one side internal carotid artery on opposite side resulted in experimental cerebral ischemia in rabbit. Measurement of thromboxane B_2 (TXB_2) and 6-Keto-prostaglandin F_(1?)(6-Keto-PGF_(1?))in rabbit plasma were done by radioimmunoassay. Blood samples were collected from the peripheral vein and internal carotid vein. The results were as follows:1. The concentration of TXB_2 in rabbit cerebral ischemia (ligation after 30 min.) was significantly (P

In order to improve the quality of nursing dissertations,we combined the teaching of nursing research with clinical internship to teach nursing dissertation writing,following the process of program input-situation experience-clinical internship-problem study-dissertation writing.Taking problem study as the leading role,students as subject,self experience as the main access and tutors as whole-process instructors,we taught and evaluated nursing dissertations at different stages.Students'interests in scientific research,enthusiasm for participation and quality of dissertations were improved effectively.95.78％ of the students showed positive attitude to this teaching method.

Lipoma preferred partner (LPP) has been identified as a protein which is highly selective for smooth muscle progenitor cells (SMPCs) and regulates differentiation and migration of SMPCs, but mechanisms of LPP expression are not elucidated clearly. The aim of the present study was to discuss the mechanisms by which LPP expression is regulated in the differentiation and migration of SMPCs induced by TGF-β1. It was found that TGF-β1 could significantly increase the expression of LPP, smooth muscle α-actin, smooth muscle myosin heavy chain (SM-MHC), and smoothelin in SMPCs. Moreover, inactivation of Rho kinase (ROK) with ROK inhibitors significantly inhibited LPP mRNA expression in TGF-β1-treated SMPCs and mouse aortic smooth muscle cells (MAoSMCs). At the same time, LPP silencing with short interfering RNA significantly decreased SMPCs migration. In conclusion, LPP appears to be a ROK-dependant SMPCs differentiation marker that plays a role in regulating SMPCs migration.

The anti-mutation effect of flesh of clam Merectix meretrix Linnaeus was studied . The increase in the rate of micronuclei of mice bone -marrow polychromatic erythrocytes induced by Cy (25 mg/kg X 2d, ip) or Ur (0. 5 g/kg X 4d , ip) was inhibited by decoctio of flesh of Meretrix merelrix Linnaeus (20g/kgX7d, ig).

Hydrolysate of Meretrix meretrix soft tissue (HMST ) (20, 10g/kg ,po.for 14d ) could markedly inhibit the increase of serum total cholesterol(TC)and triglyceride(TG),enhance the content of high density lipoprotein cholesterol(HLD-C) ;reduced cholesterol(Ch)of the liver(for 28d)in hyperlipimic quail ;and it could also reduce whole blood viscosity in normal and hyperlipemic quail (for 21d).In addition.it could suppress the platelet aggregation induced by ADP in rabbits in vitro. The results show that HMST obviously decreased blood lipid and whole blood viscosity .inhibited platelet aggregation.

Objective To evaluate the effect of physical exercise on health-related quality of life (HRQol),fatigue,depression,anxiety and body-image in breast cancer patients.Methods The Cochrane Library,Pubmed,EMBASE,CINAHL,Psycinfo,SportDiscus,CNKI and CBMdisc were searched to collect randomized controlled trials (RCTs) from 2002 to 2011 which mentioned the physical exercise to breast cancer patients.The quality of the RCTs was appraised and the data were extracted.Results In total,16 RCTs were included.Statistically significant results were found for the effect of physical exercise on HRQol,fatigue,depression,anxiety and body-image.The presence of publication bias was explored and no publication bias was detected.Sensitivity analysis were also performed and there were no significant differences with the former results.Conclusions The results indicated that physical exercise improve HRQol and psychological outcomes in breast cancer patients.Breast cancer patients should be taught to do some moderate exercises as part of their lives to promote rehabilitation.

Objective To investigate the distribution of T follicular helper(Tfh)cell subsets in hepa-titis B patients at different immune stages and to clarify the relationships between the level of CXCL13 and the distribution of Tfh cell subsets. Methods Flow cytometry analysis was performed to detect the distribution of Tfh cells. Enzyme-linked immunosorbent assay(ELISA)was performed to measure the levels of CXCL13 in ser-um samples collected from hepatitis B patients. RT-PCR and Western blot assay were used to analyze the expres-sion of CXCL13 in HepG2 and HepG2. 2. 1. 5 cells. Results The percentages of Tfh1 cells were significantly up-regulated at the immune activation(IA)stage,while those of Tfh2 cells were significantly raised at the im-mune tolerance(IT)stage. The percentages of Tfh17 cells in patients at the stage of IT were similar to those in patients at the stage of IA,but were higher than those in responders with HBsAg seroconversion(RP)or healthy controls(HC). The expression of CXCL13 was positively correlated with the percentage of Tfh2 cells. More over,hepatitis B virus(HBV)enhanced the expression of CXCL13 at both transcriptional and translational lev-els. Conclusion HBV might up-regulate the percentage of Tfh2 cells through promoting the expression of CXCL13,which resulted in the induction of immune tolerance. Elucidating the functions of Tfh1,Tfh2 and Tfh17 cells and understanding the type conversion mechanism among the three subsets are important for further researches on HBV-induced immunosuppression.

AIM: To observe the difference of actions among three traditional compl ex prescriptions with the same composition but the different dosage, including X iaochengqi Decoction, Houpudahuang Decoction and Houpusanwu Decoction, so as to provide evidence for their different clinical applications. METHODS: A series of experiments were performed, including carbon p rop ellance in small intestine, black stool excretion, cough induced by ammonia liqu or and phenol red secretion from trachea. RESULTS: Three prescriptions all enhanced carbon propellance in sma ll intestine at the high dosage, while at the low dosage only Houpusanwu Decoct ion showed the significant effect. They all shortened the latency of black stool a nd increased their total amounts in 6 hours at the high dosage, while at the low dosage Xiaochengqi Decoction and Houpudahuang Decoction had the significant p urgative action. Houpudahuang Decoction remarkably prolonged cough latency, dec reased cough times and enhanced phenol red secretion from trachea, while other t wo prescriptions had no obvious effects. CONCLUSION: Such results showed that Xiaochengqi Decoction had the better purgative action, while Houpusanwu Decoction regulated Qi strongerly, a nd Houpudanghuang Decoction showed the marked actions of relieving cough and re solving phlegm.

Objective To understand information of initial sexual behavior age of females in Linxiang district of Yunnan province and investigate HPV and HPV vaccine awareness of rural women ,health workers and government staff .Methods During Decem-ber 2009 to December 2010 ,750 cases aged from 15 to 59 years included rural women ,government officials and medical staff in Linxiang district were randomly selected and investigated .Results The age of first sex was (22 .4 ± 2 .3) years old .The number of sexual partners was (1 .2 ± 0 .3) and proportion of premarital sex was low (8 .8% ) .HPV vaccine awareness of rural women was 8 .59% .The proportion of people who knew HPV vaccine could prevent cervical cancer was 6 .57% .The proportion of people who wanted to be vaccinated was 97 .66% .The cost of HPV vaccine which rural women could accept was 300 yuan .Conclusion Women in Linxiang area of Yunnan province have less premarital sex and lack of awareness in HPV .