1.The introduction and enlightenment of the teaching philosophy and methods of evidence-based medicine for foreign graduate students
Hanmei TANG ; Yanyan FU ; Shaoju MAO ; Weixuan SUN ; Zhilin LIAO ; Jianni LI ; Hongheng QIU ; Jinyun ZHU ; Peng HUANG
Chinese Journal of Medical Education Research 2016;15(2):130-134
Aiming at evidence-based medicine course of graduate students, foreign medical colleges and universities usually took in the teaching philosophy that emphasized stages (focusing on knowledge ac-cumulation) and practicality (focusing on ability training). And they put forward a lot of teaching methods which implemented the conception, such as problem-based learning, journal club, blended learning approach, online learning platform, concept mapping etc. In order to provide a useful reference for the domestic teaching reform and development, this paper introduced the teaching philosophy and related teaching meth-ods mentioned above.
2. Impact of KIT D816 mutation on salvage therapy in relapsed acute myeloid leukemia with t(8;21) translocation
Benfa GONG ; Yehui TAN ; Aijun LIAO ; Jian LI ; Yueying MAO ; Ning LU ; Yi DING ; Erlie JIANG ; Tiejun GONG ; Zhilin JIA ; Yu SUN ; Bingzong LI ; Shuchuan LIU ; Juan DU ; Wenrong HUANG ; Hui WEI ; Jianxiang WANG
Chinese Journal of Hematology 2018;39(6):460-464
Objective:
To evaluate the impact of KIT D816 mutation on the salvage therapy in relapsed acute myeloid leukemia (AML) with t(8;21) translocation.
Method:
The characteristics of the first relapsed AML with t(8;21) translocation from 10 hospitals were retrospectively collected, complete remission (CR2) rate after one course salvage chemotherapy and the relationship between KIT mutation and CR2 rate was analyzed.
Results:
68 cases were enrolled in this study, and 30 cases (44.1%) achieved CR2. All patients received KIT mutation detection, and KIT D816 mutation was identified in 26 cases. The KIT D816 positive group had significantly lower CR2 compared with non-KIT D816 group (23.1%
3.Transformation mechanism of carbon tetrachloride and the associated micro-ecology in landfill cover, a typical functional layer zone.
Yongqiong WANG ; Zhilin XING ; Shangjie CHEN ; Xia SU ; Kun CAO ; Ludan CAO ; Shushu LIAO ; Langlang DONG ; Shuo AI ; Tiantao ZHAO
Chinese Journal of Biotechnology 2022;38(5):1874-1888
Landfill is one of the important sources of carbon tetrachloride (CT) pollution, and it is important to understand the degradation mechanism of CT in landfill cover for better control. In this study, a simulated landfill cover system was set up, and the biotransformation mechanism of CT and the associated micro-ecology were investigated. The results showed that three stable functional zones along the depth, i.e., aerobic zone (0-15 cm), anoxic zone (15-45 cm) and anaerobic zone (> 45 cm), were generated because of long-term biological oxidation in landfill cover. There were significant differences in redox condition and microbial community structure in each zone, which provided microbial resources and favorable conditions for CT degradation. The results of biodegradation indicated that dechlorination of CT produced chloroform (CF), dichloromethane (DCM) and Cl- in anaerobic and anoxic zones. The highest concentration of dechlorination products occurred at 30 cm, which were degraded rapidly in aerobic zone. In addition, CT degradation rate was 13.2-103.6 μg/(m2·d), which decreased with the increase of landfill gas flux. The analysis of diversity sequencing revealed that Mesorhizobium, Thiobacillus and Intrasporangium were potential CT-degraders in aerobic, anaerobic and anoxic zone, respectively. Moreover, six species of dechlorination bacteria and eighteen species of methanotrophs were also responsible for anaerobic transformation of CT and aerobic degradation of CF and DCM, respectively. Interestingly, anaerobic dechlorination and aerobic transformation occurred simultaneously in the anoxic zone in landfill cover. Furthermore, analysis of degradation mechanism suggested that generation of stable anaerobic-anoxic-aerobic zone by regulation was very important for the harmless removal of full halogenated hydrocarbon in vadose zone, and the increase of anoxic zone scale enhanced their removal. These results provide theoretical guidance for the removal of chlorinated pollutants in landfills.
Bacteria/metabolism*
;
Biodegradation, Environmental
;
Carbon Tetrachloride/metabolism*
;
Methane/metabolism*
;
Waste Disposal Facilities