Objective:To explore whether there will be attentional bias under the condition of fear,disgust and positive emotional arousal. Methods:Totally 96 college students were selected as participants in this study,inclu-ding 60 males and 36 femalesaged 18-22 years. Affective priming videos were adopted to prime participants'emo-tion (dread,disgust,joviality),and then they named the color of the cognitive words (positive words,negative words,neutral words)as quickly and accurately as possible. Reaction time and correctness were recorded. E-prime was adopted to write the experimental program. The reaction time for neutral words was baseline. Results:The aver-age reaction time (RT)of color-naming tasks of negative words were shorter than that of neutral words after fright-ening affective priming[(648 ±118)ms vs. (743 ±124)ms,P<0. 01]. Furthermore,the average RT of color-na-ming tasks of positive words were also shorter than that of neutral after frightening affective priming[(683 ±123) ms vs. (743 ±124)ms,P<0. 05]. The average RT of color-naming tasks of negative words were shorter than that of neutral words after disgusting affective priming[(579 ±86)ms vs. (720 ±101 )ms;P<0. 01 ]. Moreover,the av-erage RT of color-naming tasks of positive words were shorter than that of neutral words after delighted affective priming[(634 ±122)ms vs. (716 ±141)ms;P<0. 01]. Furthermore,the average RT of disgusting priming group-were shorter than the frightening priming grouptowards negative information[(579 ±86)ms vs. (648 ±118)ms,P<0. 05 ]. Conclusion:It suggests that the youth perform more obvious attentional bias towards negative information after disgusting affective priming than the frightening one. Frightening affective priming could evidently induce the attentional bias on positive information.

Objective To investigate the effective route and proper method in transfecting gene into liver graft mediated by adeno-associated virus vector.Methods Three routes including hepatic artery,portal vein and hepatic artery+portal vein,and 3 methods,i.e.routine,circulation and clamping were employed for infusion.The best infusion route and method of gene transfection into liver graft were determined by observing the color change of liver and detecting liver function and transfoetion rate of liver cells.The safety of these methods was evaluated.Results In all the infusion procedures,the color of the liver grafts turned from red to white,no apparent color differenee of the livers and no enlargement nor mottling were observed under surgical microscope.The liver color was back to normal immediately after blood flow was restored.No significantly statistical differences of the ALT values were observed among all the groups(F=0.343,1.265,0.055,P＞0.05).Adeno-associated virus vectors coding for the enhanced green fluorescence protein(AAV2-EGFP)were successfully transfected into liver cells by the 3 infusion routes 1 week later,and the difierences of transfection rates via the 3 routes had no statistical significance(F=0.080,0.091,0.045,P＞0.05).The transfoction rate of AAV2-EGFP was the highest at any time points when using the clamping method,and then followed by circulation method and routine method,with statistical differenee(F=3.880,2.976,5.129,P＜0.05).The transfection rates of AAV2-EGFP were increased progressively and peaked at the 6th week,and then they were decreased gradually.Conclusions Infusion via hepatic artery is the effective route for gene transfection and clamping the vessels can elevate the transfection rate of AAV2-EGFP.All procedures were performed without detectable liver injury.The transfection of gene into liver graft mediated by adeno-associated virus vector is a slow and persistent process.

Objective To investigate the psychological measurement index for the effectiveness and discrimination of drug packaging (color code) in the medicine for self-aid and mutual-aid .Methods The classical priming paradigm was used to inves-tigate whether there were influences on the recognition accuracy (ACC) and reaction time (RT) by memorizing the characteris-tics of three series of color code ,with a 2 (relevant or irrelevant priming )× 3 (color code categories) within subject experimen-tal design .Results ①The recognition (RT ) of the relevant-priming group was 1 387 .18 ms ,compared with the irrelevant-priming group 1 475 .12 ms .The independent two-sample t-test showed statistically significant difference between the relevant-priming and irrelevant-priming groups (t=2 .264 ,P=0 .02) .②The analysis of variance of factorial design showed a significant difference among the three series in the recognition (RT) (F=9 .945 ,P<0 .001) .The priming effect was significant in color code 1 and 3 ,while not significant in color code 2 .③The recognition (RT) in color code 2 was different by the judgment task :using directives< class-1 categorical attributes < class-2 categorical attributes ,consistent with the order of difficulty of the judgment task .The P value for the priming effect was 0 .03 ,0 .21 and 0 .7 in the judgment task for using directives ,class-1 cat-egorical attributes and class-2 categorical attributes .The priming effect was significant in using directives ,while not significant in class-1 categorical attributes and class-2 categorical attributes .Conclusion The priming effect of the characteristic of the color code is significant ,and the characteristic of the color code can promote the process of memorization of the medicine for self-aid and mutual-aid .