Objective:To evaluate the effects of repairing incomplete c left palate with double island mucoperiosteal flap. Methods:Inco mplete cleft palate was repaired with double island mucoperiosteal flap in 12 ca ses. Two island mucoperiosteal flaps were transferred to the oral side and nasal side of soft palate respectively to prolong soft palate. After operation the pa tients were followed up for 3~6 months,the vitality of the flaps, the degree of prolonged soft palate and the change of vocalization were surveyed. Resu lts:All transferred flaps were survived. No complications like perforati on, cleft recurrance, infection, necrosis etc happened. The soft palate was prol onged obviously in all cases. Vocalization of the patients was improved to a cer tain degree. Conclusions:Double island mucoperiosteal flap is fe asible in the repair of incomplete cleft palate.

Objective To explore the effect of the submental island flap in reconstruction of facial defects. Methods Six patients (4 men and 2 women) with lesions in maxillofacial regions were registried in our clinical database. Patients age ranged from 44 to 78 years, lesions were distributed in left face (2 cases), left parotid area (1 case), right parotid area (1 case), and right face (2 cases). The pathologic diagnoses were dermoid cyst with local skin necrosis (1 case ), sebaceous cyst with inflammation and necrosis (1 case), canceration of epithelial tumor (1 case) and squamous cell carcinoma (3 cases). The benign tumors were resected and malignant tumors were extensively resected with neck dissection. The defects (minimum 4.0 cm?3.0 cm, maximum 6.0 cm?6.0 cm ) after surgical resection were reconstructed with the submental island flap. The flap area ranged from 4.5 cm? 3.0 cm to 6.0 cm?4.5 cm. The flap pedicle included submental artery (3 cases) and both submental artey and facial artery ( 3 cases ). The length from end of flaps to origin of pedicle was 6 cm in 3 cases,9 cm in 2 cases and 11 cm in 1 case. Results The flaps survived in all six cases, no obvious difference in colour was found between the flaps and facial skin; no infection at recipient site, unhealed and necrosis occurred. The wounds of donor sites were primarily healed, and the scar of donor site was out of sight when looked with eyesight parallel to the ground. Conclusion It is an ideal method to reconstruct facial defects with submental island flap.

Objective: To establish the model of palatine mucoperiosteal expansion and observe a new possible treatment approach to soft tissue defect of cleft palate. Methods: Expanders of 10ml were implanted beneath the palatine mucoperiosteum in 4 dogs and injected 1.0ml 0.9% NaCl at the same time. 20 days after the placement of the expander, the expander was expanded by 1.0-1.5ml every 7 days again. The dogs were killed after the expanders reached their capacity. All the specimens were performed histomorphometric and histological examinations. Results: Histological results showed the increase in the layer of cell and the number of collagen fibra and blood vessel. All the specimens of expanded tissue showed the formation of extra palatine mucoperiosteal tissue. Conclusion: Expansion technique is able to form extra palatine mucoperiosteal tissue,which could be a new restoration approach to soft tissue defect of cleft palate and others.

Food-born trematodes which parasitize liver, lung and intestine cause various trematodes diseases. There are a few common trematodes such as Clonorchis sinensis, Fasciola, Opisthorchis, Paragonimus, and Echinostoma. This review provides recent progress in techniques and methods for their immunodiagnosis. These methods are mainly used to diagnose with excretory-secretary antigen, recombinant antigen and antibodies of hosts, by means of enzyme-linked immunosorbent assay, immunoblotting, immunefluorescence, immunehistochemistry, proteomics and a lateral flow detection system so on. It is beneficial to improve diagnostic and accuracy rates when all this methods are used synthetically.

OBJECTIVETo summarize the techniques and evaluate the therapeutic effect of posterior fixation and fusion in the treatment of Os odontoideum complicated by atlantoaxial dislocation.

METHODSFrom March, 2007 to October, 2010, 10 patients with Os odontoideum (including 6 male and 4 female patients aged from 20 to 65 years, mean 39.8 years) were treated in our hospital. Before and after the operation, the patients underwent X ray, CT and MRI examinations to measure and evaluate the degree of dislocation and neural compression. After preoperative traction for 1-2 weeks, all the 10 patients showed deductible atlantoaxial dislocation. Through a posterior approach, Atlantoaxial pedicle screws fixation were performed in 9 cases, and C2/3 pedicle-Occiput screw fixation was performed in 1 case. All the patients wore cervical collars as external support for 3 months after the operation.

RESULTSThe mean operative time was 3 h in these patients with a mean intraoperative blood loss of 420 ml. The symptoms were relieved after the surgery in all the patients, who showed no neck pain or neurological defects. The patients were followed up for 6 to 52 months (mean 22 months), and bony fusion was observed in all the 10 cases within 6 to 8 months without such complications as internal fixation failure or redislocation of the atlas.

CONCLUSIONPatients with Os odontoideum complicated by atlantoaxial dislocation should undergo surgical stabilization to avoid severe neurological injury. Pedicle screw instrument in the atlas allows restoration of the spinal stability, short-segment fusion, and maximal preservation of the mobility of the neck.

Adult ; Aged ; Atlanto-Axial Joint ; injuries ; Bone Transplantation ; Female ; Fracture Fixation, Internal ; methods ; Humans ; Joint Dislocations ; complications ; surgery ; Joint Loose Bodies ; complications ; surgery ; Male ; Middle Aged ; Odontoid Process ; pathology ; Spinal Fusion ; methods ; Treatment Outcome ; Young Adult

The rapid and accurate authentication of traditional Chinese medicines(TCMs)has always been a key scientific and technical problem in the field of pharmaceutical analysis.Herein,a novel heating online extraction electrospray ionization mass spectrometry(H-oEESI-MS)was developed for the rapid and direct analysis of extremely complex substances without the requirement for any sample pretreatment or pre-separation steps.The overall molecular profile and fragment structure features of various herbal medicines could be completely captured within 10-15 s,with minimal sample(＜0.5 mg)and solvent consumption(＜20 μL for one sample).Furthermore,a rapid differentiation and authentication strategy for TCMs based on H-oEESI-MS was proposed,including metabolic profile characterization,characteristic marker screening and identification,and multivariate statistical analysis model validation.In an analysis of 52 batches of seven types of Aconitum medicinal materials,20 and 21 key compounds were screened out as the characteristic markers of raw and processed Aconitum herbal medicines,respectively,and the possible structures of all the characteristic markers were comprehensively identified based on Com-pound Discoverer databases.Finally,multivariate statistical analysis showed that all the different types of herbal medicines were well differentiated and identified(R2X＞0.87,R2Y＞0.91,and Q2＞0.72),which further verified the feasibility and reliability of this comprehensive strategy for the rapid authentication of different TCMs based on H-oEESI-MS.In summary,this rapid authentication strategy realized the ultra-high-throughput,low-cost,and standardized detection of various complex TCMs for the first time,thereby demonstrating wide applicability and value for the development of quality standards for TCMs.

The dosage， dose and administration method of decoctions are important factors affecting the efficacy of prescriptions. By analyzing 35 decoction formulas from the Ming and Qing dynasties within the Catalogue of Ancient Classic Famous Formulas （First Batch）， it was found that the average dosage was equivalent to about 65 g， of which 71.4% （25/35） of the prescriptions had a dosage ≤60 g. And among them， the dosage of decoctions in the Ming dynasty was significantly smaller than that in the Qing dynasty. Considering the characteristics of formulas in Song dynasty， it is believed that decoctions in Ming and Qing dynasties were influenced by the popular use of decoctions during the Song， Jin， and Yuan dynasties. Some decoctions recorded a dosage of one dose instead of one day， which was more evident in the Ming dynasty. However， by the Qing dynasty， the usage of prescriptions with a dosage of one day gradually became more common. Therefore， in the practical research and application of classic famous formulas from the Ming and Qing dynasties， it is advised to pay attention to the difference between the dosage， one dose and the daily dosage. It is necessary to determine whether to double the dosage of the original formula based on the actual use， in order to ensure the clinical efficacy.

By reviewing ancient materia medica and combining with modern literature， the textual research of Magnoliae Flos has been conducted to verify the name， origin， producing area， harvesting and processing methods， in order to provide basis for the selection and use of this herb in the development of famous classical formulas. After the textual research， it could be seen that the correct name of Magnoliae Flos was Xinyi in the past dynasties， meaning spicy flower buds. The main original plants used in past dynasties are Magnolia denudata and M. biondii. The history of the research on its scientific name in recent times is complicated， many foreign scholars have given several different scientific names， but most of them are inconsistent with the actual situation of Magnoliae Flos used in ancient China， because foreign scholars failed to collect the original plants of Magnoliae Flos for accurate identification. Before the Ming dynasty， Magnoliae Flos was mainly produced in Shaanxi， and then the recorded production areas gradually increased. After the founding of the People's Republic of China， the products produced in Henan named M. biondii were highly respected， and Henan was regarded as authentic producing area， and because of the collection and distribution through Yuzhou， it was customarily called Huichunhua. In ancient times， the harvesting period of Magnoliae Flos mostly concentrated in the first and second months of the lunar calendar， and the flower buds of M. biondii were also recommended to be used as medicine， but nowadays the flower buds are mostly collected in winter and spring， and those with dry buds， large size， yellow-green color， tight inner petals， fragrant smell， and no impurities are preferred. In the past dynasties， raw products were the mainstream， and there were also frying， soaking and so on. Based on the results， it is suggested that the flower buds of M. biondii should be used in the development of famous classical formulas. If the original formula does not specify the processing requirements， the raw products can be used as medicine.

By consulting ancient materia medica， medical books， prescription books and modern literature， this paper systematically combed and reviewed the name， origin， scientific name evolution， producting area， quality evaluation， medicinal parts， harvesting and processing and traditional efficacy of Lasiosphaera Calvatia. The results show that Mabo was first recorded in Mingyi Bielu. Since then， all dynasties have taken Mabo as a legitimate name. Before the Song dynasty， only Calvatia lilacina was used as the original plant of Lasiosphaera Calvatia， which was expanded after the Song dynasty with the appearance of C. gigantea， Lasiosphaera fenzlii， Bovistella radicata and other varieties. Until modern times， there was an addition of Lycoperdon perlatum， L. pyriforme and other original plants of Lasiosphaera Calvatia. Since 1975， the original plant of Lasiosphaera Calvatia in various regulations and academic monographs has been basically uniform for C. lilacina， Lasiosphaera fenzlii and C. gigantea. Resource of the medicinal fungus was widely distributed in China and was mainly wild. From ancient times to the present， the medicinal parts of Lasiosphaera Calvatia are all fruiting body， which is harvested in summer and autumn， and its processing method was to take powder in ancient times， but to cut blocks in modern times. In recent times， its quality has been summarized as large， thin-skinned， intact， full， loose-bubbled and elastic. The medicinal efficacy has been developed from very good for all scores， and after the Ming and Qing dynasties， it is consistent with the 2020 edition of Chinese Pharmacopoeia， with the efficacy of clearing the lung， promoting pharynx， relieving fever and hemostasis， mainly treating cough aphonia， throat obstruction and pharyngeal pain， vomiting blood， epistaxis， hemoptysis， and external treating sores and bleeding from cuts and wounds. Based on the results of herbal textual research， it is suggested that C. lilacina is the first choice for the origin of Lasiosphaera Calvatia involved in famous classical formulas， and it is processed into block or powder for medicine.

This article has systematically reviewed the name， origin， scientific name， producing area， quality evaluation， harvesting and processing methods of Polygonati Odorati Rhizoma（POR） by consulting the materia medica， medical books， prescription books and modern literature， in order to provide a reference for the development of famous classical formulas containing POR. Yuzhu was first recorded in the Shennong Bencaojing under the name of Nyuwei. After that， Weirui was used as the rectification name in the subsequent dynasties， and in recent times， the name of Yuzhu is mostly used in materia medica and prescription books. In ancient times， there were different names for Yuzhu， such as Nyuwei， Weiwei and Weirui. The names of the three are similar and there was a mixed use of the same name and foreign matter in history. In the Tang dynasty， SU Jing listed Nyuwei with the effect of curing dysentery in the intermediate of herbal part of Xinxiu Bencao according to its different efficacy. However， based on Shennong Bencaojing， Mingyi Bielu and the different energy efficiency of medical prescriptions， SU Song of the Northern Song dynasty believed that the three were medicinal materials of different origins. In short， the names of the three have been unclear in history for a long time. According to the development of the time line， this paper examines the names and realities of the three， and concludes that the two（Weiwei and Weirui） are the same medicinal material， that is， Polygonatum odoratum of Liliaceae， and the Nyuwei is Clematis apiifolia of Ranunculaceae， and the source relationship of the three is clarified. The mainstream source of Yuzhu used in the past dynasties was the rhizome of P. odoratum， which was widely distributed in the wild and has a large amount of resources. The origins of Yuzhu recorded in ancient times were mainly Taishan in Shandong， Chuzhou and Shuzhou in Anhui， and Hanzhong in Shaanxi， in modern times， it was produced in northern Hebei and Shaoyang in Hunan with high quality， and in the modern times， Jiangbei Yuzhu from Haimen in Jiangsu， Anyuzhu from Nanling， Anqing and Tongling in Anhui， Guanyuzhu from Fengrun， Yutian， Zunhua， Huailai in Hebei and Suizhong， Jinxi， Jianchang， Lingyuan， Liaoyang， Haicheng， Gaiping in Liaoning， Xiangyuzhu from Shaoyang in Hunan are the authentic medicinal material. In ancient times， the quality of Yuzhu was good if it was fat and white， while in modern times， it is better with thick roots， bright yellow color， soft texture， no stiff skin and no oiliness. In ancient times， the origin processing of POR was mostly dried in the shade， but in modern times， it is mostly sun-dried or dried after steaming and rubbing. The ancient processing was mostly scraped off the skin and soaked in honey water and then steamed through， while the modern one is mostly washed and cut into thick slices for raw use. Based on the conclusion of the herbal textual research， it is suggested that the rhizome of P. odoratum of Liliaceae be used as the source for the development of famous classical formulas， and the corresponding specifications be selected according to the processing requirements of the prescription. In view of the Yiweitang in Wenbing Tiaobian， which uses the method of frying fragrance to achieve the effect of fragrant refreshing the spleen， it can be processed by referring to the stir-frying method in the current version of Chinese Pharmacopoeia.