Objective To probe distribution and excreting cells of type I collagen in rat heart. Methods Expression of cardiac collagen I was detected using immunohistochemistry and in situ hybridization. Results Type I collagen was distributed in myocytes,and it encased each cardiac cell.The densities of collagen network in difference cardiac part were of disparity.Type I collagen was all expressed in fibrcyte and smooth muscle cells and endothelial cells in blood vessels of cardiac tissue.Conclusion The type I collagen is a main component of cardiac fibrous network,and its excreting cells are multifocal.

Based on literature review, questionnaire survey, Interact investigation, experts interview and seminars the paper analyzes the status quo of the information literacy education and existing problems in traditional Chinese medicine colleges and universities, proposes constructive advices on carrying out information literacy education, moreover, provides a information literacy education standard for traditional Chinese medical colleges and universities and initially constructs a comprehensive information literacy education system.

Objective To study the expression of telomerase(TMA) in myocardial tissue of myocardial ischemia in rat. Methods Sixty Spraque-Dawley rats were randomly divided into A,B, C three groups,then each group was randomly divided into control group and testing group.Two myocardial ischemia groups were injected isoprenaline(8 mg/kg),and two control groups were injected distilled water(8 mg/kg) in abdominal cavity.For the last myocardial ischemia group the coronary artery was ligated.The histological and immunohistochemical method were used for observing cardiac structural changes,with HE staining,and changes of TMA in myocardial tissue after myocardial ischemia in rat.The TMA contents were measured by image analysis. ResultsCompared with the respective control group,the expression of TMA in the three myocardial ischemia groups increased obviously(P

Objective To investigate the spatio-temporal expression of connexin 43(Cx43) in the cultured ventricular myocardial cells of neonatal rats. Methods The techniques of Immunocytochemistry(ICC) and immuno-electron microscopy were used to detect the Cx43 expression in the cultured rat ventricular myocardial cells on the(2nd),(4th),(8th),(10th),(12th),(16th),(20th,)(26th) and(30th) days. Results Cx43 expression was detected in the cultured ventricular myocardial cells on the 2(nd) day,and the Cx43 granules were located largely in the cellular cytoplasm and membrane.The punctiform granule of the cellular cytoplasm decreased and the expression of Cx43 was located mainly in cellular membrane junction on the 4(th) day.The expression of Cx43 increased in cellular membrane junction on the 10(th) day,and the morphology of Cx43 expression was chain-and strip-like.There were not obvious changes in the following days.The expression of Cx43 on the 30(th) day was derangement.Conclusion The spatio-temporal expression of Cx43 in the cultured ventricular myocardial cells of neonatal rats changed with the cultural time in terms of location and quantity.It was in accordance with the growth and development of the cultured ventricular myocardial cells.

The aim of information literacy(IL) education is to cultivate students' ability to continuously update and improve their expertise.An overall IL education system contains three parts-information technology education and its' combination with medical science,information retrieval education and its' combination with medical science,and the building of good study environment based on information.

Objective To investigate cell type and size of fetus atrioventricular node. Methods Using the technique of paraffin section, H-E staining, the cellular morphology and size of atrioventricular node in fetus hearts were observed under microscope, and quantitied by HIPAS-1000 computerized image analytical instrument. Results The cell types of atrioventricular node in fetue hearts were composed of light, dark and transitional cells. The morphology of light and dark cells were spindle-shaped or ellipsoid, but a few cells were polygonal. The shape of transitional cells was short column. The nuclei of light cells and transitional cell were larger than that of dark cells.Conclusion Pacemaker cell of atrioventricular node in fetus heart includes light and dark cells which could present different function types of pacemaker cell. [

Objective To research the spatiotemporal pattern of N-cadherin expression during development of rat cardiomyocytes and age-related changes of N-cadherin expression. Methods Using immunohistochemical method,myocardial N-cadherins distribution was investigated in fetal rat and postnatal development(1 postnatal day to old rat),and quantitied by HIPAS-1000 computerized image analytical instrument. Results The expression of N-cadherin was located in myocardium of atrial and ventricule and septum interventriculare and papillary muscles.The N-cadherin immunolabeling was found in myocyte membranes and within cytoplasm in fetal rat heart.From neonatal to infant rat,the pattern of N-cadherin immunolabeling changed progressively,from being dispersed over the entire cell surface as in the fetal to the transverse terminals of the myocytes,toward the distribution within the intercalated disk.From young to old rat heart,the typical N-cadherin was located in transversely orientated intercalated disk.The percentage of N-cadherin immune postive area in rat ventricular myocardium showed a progressively changement with age.Conclusion The present paper demonstrated that the N-cadherin expression exists progressively with ages and the changing pattern of N-cadherin is closely related with development of the intercalated disk in rat myocardium.The mechanical coupling provided by adherens junctions is essential for the stable cell-cell contact.

Objective To investigate age-related changes of type Ⅰ and Ⅲ collagen in rat heart. Methods Twenty-one male Wistar rats were divided randomly into infanct,young and old group.Types Ⅰ and Ⅲ collagen expresson of different age hearts were studied by SP immunohistochemical methods. Results 1.Type Ⅰ and Ⅲ collagen all constitute thick fiber and fibril.The fibrils encircled every cardiac muscle and formed collagen net each other.The thick fibers being plaque were located among cardiac muscle groups.The content of type Ⅰ collagen was more than that of type Ⅲ.2.There were distinct expression difference of type Ⅰ and Ⅲ collagen in different aging rat hearts.The collagens distributed densely and evenly in infancy rat heart,and loosely and uniformly in young rat heart,and the contents were increased distinctly with heterogeneous distribution in old rat heart.The cardiac collagen was growing from infanct to old rat,and rapid progress of cardiac collagen was seen in young rat. 3.The content of collagen in right ventricle was more than that of left ventricle of infanct heart.The collagen in all parts of the heart is not of difference both in yound and old rat. Conclusion The contents of type Ⅰ collagen is more than that of type Ⅲ collagen.The two types collagen are increasing with growing.;

Objective To research pacemaker cells outside conductive system of rat and mouse heart. Methods Paraffin and frozen serial sections (stained with HE, Masson, cholinesterase technique), and ultrastructural sections, from atrial tissue of 10 rats and 15 mice were observed by light and electron microscopy to find latent pacemaker-like cells. Results Some round or elliptical cells were scattered and irregularly detected in right and left atrial tissue of rat and mouse. Their nuclei were larger, plasma was clearer and cellular organelles were fewer than those of working myocyte. These cells were similar to pacemaker cells in sinoatrial node.Conclusion There are some pacemaker-like cells in right and left atrial tissue of rat and mouse. The finding can provide a morphological basis for ectopic beat of atrium.

Objective To study the relation between vascular endothelial growth factor(VEGF) and the degree of coronary artery stenosis as well as the formation of collateral circulation in patients with acute myocardial infarction (AMI). Methods Seventy-six cases of AMI had undertaken selective coronary angiography on the third to seventh day after admission and also at six months afterwards in order to define the degree of coronary artery stenosis and the presence of collateral circulation. A blood sample of 5 mL was taken in each patient before the first coronary angiography to test for the level of VEGF by enzyme linked immunosorbend assay. Results The level of VEGF in patients with coronary artery stenosis less than 50%, 50%~75% and greater than 75% were (97.6?17.3) ng/L, (241.6?28.9) ng/L and (391.7?48.4) ng/L respectively (P