Objective To investigate the efficacy of remifentanil-propofol-atracurium combined general anesthesia for cesarean section.Methods One hundred and twenty ASA Ⅰ or Ⅱ parturients scheduled for elective cesarean section were enrolled in the study.Anesthesia was induced with iv injection of remifentanil 1.0 μg/kg,propofol 2.0 mg/kg and atracurium 0.4 mg/kg and maintained with iv infusion of remifentanil 9.0 μg· kg-1 · h-1,propofol 12.0 mg· kg-1 · h-1 and intermittent iv doses of atracurium 0.3 mg/kg.Hemodynamic parameters were recorded during anesthesia.Blood samples were taken from the umbilical artery after delivery for blood gas analysis.Apgar score and assessment of neonatal neurological behavior score were recorded.The emergence time,time of recovery of bowel function and intraoperative awareness were also recorded in parturients.Results The blood gas indexes,Apgar score and assessment of neonatal neurological behavior score in neonates and hemodynamic parameters during anesthesia in parturients were within the normal range.The emergence time was (5.0-± 0.5) min.No severe adverse reactions was found during the perioperative period.The postoperative followup showed that no intraoperative awareness occurred and the time of recovery of bowel function was (16.2 ± 0.7)h.Conclusion The efficacy of remifentanil-propofol-atracurium combined general anesthesia is good for cesarean section without obvious adverse effects on parturients and neonates.

OBJECTIVETo study optical intrinsic signal (OIS) imaging of peri-infarct depolarizations (PIDs) in mice and investigate the influence of knockout of glial fibrillary acidic protein and vimentin on PIDs.

METHODSGFAP(⁺/⁺)Vim(⁺/⁺) mice and GFAP(⁺/⁺)Vim(⁺/⁺) mice were subjected to MCAO by standard intraluminal filament method. The main characteristics of PIDs in 4 h were studied by 4-wavelength OIS imaging technique.

RESULTSPIDs were identified as consistent, red and blue interaction waves in the cortical reflectance that slowly propagated peripherally from the origin site. There were 5 patterns of PID propagation, namely rostro-caudal, latero-medial, caudo-rostral, contralateral and medial-lateral. No significant differences were found in PID frequency, propagation patterns, velocity or duration time between the two groups (P>0.05).

CONCLUSIONThe 4-wavelength OIS system allows acquisition of high temporal-spatial resolution color images for analyzing temporal-spatial characteristics of PIDs in detail. Knockout of GFAP and vimentin do not affect PIDs in 4 h following middle cerebral artery occlusion.

Animals ; Glial Fibrillary Acidic Protein ; Infarction, Middle Cerebral Artery ; pathology ; Mice ; Mice, Knockout ; Nerve Tissue Proteins ; genetics ; Optical Imaging ; Vimentin ; genetics