The expression of C-myc, K-ras and H-ras oncoproteins in lymphocytesand monocytes, and NK subsets were studied in 21 patients with Behcet's syndrome (BS)and 40 healthy individuals. CD16~+,57~- cells of NK from BS patients were much decreasedwhen compared to the control. The positive percentages of three kinds of oncoprotein in BS lymphocytes and C-myc and K-ras oncoproteins in BS monocytes were significantly higher thanthat of the control. Positive correlations were found not only between C-myc expression oflymphocytes and serum ?-globulin%, IgM and IgG levels in BS, but also between C-mycexpression of monocytes and ESR. Expression of K-and H-ras oncoproteins in lympho-cytes and monocytes from BS patients were found to be negatively correlated with serumIgA level. Negative correlation was also found between C-myc expression in monocytesand CD16~+,57~- subset of NK. The significance of these results and the possible correlationsbetween BS and autoimmunity were discussed. It was suggeted that autoimmunity mightbe an important mechanism underlying BS.

mbus in IVC. Interventional treatment can prolong the survival time and promote the quality of life.

Objective To study the changes of insulin-like growth factor-1(IGF-1) in serum of patients with obstructive jaundice.Methods The clinical data of 20 patients with obstructive jaundice were collected and the measurement of serum TNF-?,ALT, ALP, endotoxin and IGF-1 were performed. Results The serum IGF-1 in obstructive jaundice was significantly lower than that in gallbladder stone(P

AIM:To investigate the kinetics of tumor necrosis factor alpha(TNF-?)in an animal model of chronic P.aeruginosa biomembrane associated with lung infection.METHODS:Rats were challenged with 0.1 mL of PAO579(1012 CFU/L)in alginate beads or 0.1 mL of planktonic PAO579(1012 CFU/L).After challenging for 3,7 and 14 d,bacteriological and pathological features,and TNF-? expression in lung tissue were observed.RESULTS:(1)CFU/lung in alginate beads group was significantly higher than that in planktonic bacteria group(P

Objective To analyze the causes of recurrent hemoptysis after interventional treatment and to provide the reference to improve the interventional radiology technique in dealing with the hemoptysis.Methods From Oct.2000 to Oct.2008,repeated interventional procedure was carried out in 22 patients with recurrent hemoptysis after initial interventional treatment.After the bronchial arterial angiography and collateral branch arterial angiography were performed,embolization of diseased arteries with gelatin sponge particle,PVA particle or microcoil was conducted.Results Of 22 patients,re-canalization of the bronchial arteries was seen in 4,blood supply from additional bronchial artery other than from the already embolized bronchial artery in 3,and blood supply from the collaterals in 15.Technical success of the second interventional treatment was achieved in all the 22 cases.Bleeding ceased immediately after the second interventional treatment and no recurrent hemoptysis occurred during the follow-up period of 3 months to 4 years.Conclusion Complete embolization of all arteries related to the hemoptysis together with actively dealing with the primary disease can effectively improve the successful rate of interventional treatment for the massive hemoptysis.

Objective To investigate the effects of different durations of methamphetamine abuse on spatial cognitive processing.Methods The mental rotation task was used to evaluate the spatial processing function of 35 methamphetamine abusers and 30 healthy subjects.The methamphetamine abusers are divided into 2 groups according to their abuse durations,group 1 contains 16 abusers with an average duration of 1 year and group 2 contains 19 abusers with an average duration of 3 years.Participants were asked to judge the right hand/food or the left hand/food of the experimental stimuli at difference angles.The reaction time (RT) and the accurate rate (AR) of the mental rotation task were recorded.Results Compared with the control group,RT of group 1 methamphetamine abusers was longer at the angle of 0°((1469±318)ms) and 180°((1718±412)ms) (P＜0.05),RT of group 2 was longer at the angle of 0°((1466±243) ms),60°((1497±294) ms) and 180°((1708±288) ms) (P ＜0.05).And the AR of methamphetamine abusers was higher at every angle (P＜0.05).Conclusion Long-term dependence on methamphetmine can damage the abuser's spatial processing function.

Objective Through the research of the disposal of clinic laboratory result information,the paper explore the methods of building up laboratory's online management system and network information system.Methods This project began with the analysis of the need of laboratory and applies computer to simulate the manual disposal process.The system framework was separated into many sub parts according to its function.VB,etc were used to program.Results The project has been gained successfully in the online managemert of two analyzers and the management of data network consisting of three laboratories.Conclusion The project has established a practical and reliable in-formation management system which matches the work mode in clinical laboratory and has novelty functions.

Objective To explore the efficacy and safety of bedside continuous blood purification (CBP) in the treatment of critically ill neonates.Methods Totally ten critically ill neonates were hospitalized in Department of Neonatal Intensive Care Unit (NICU) in Shanghai Children's Hospital from June 2011 to May 2015, and managed with CBP treatment.The indications of CBP therapy were multiple organ dysfunction syndrome (MODS) failed to conventional treatment or combined with acute renal failure (ARF).The model for CBP was continuous veno-venous hemofiltration dialysis (CVVH).The clinical outcomes included blood electrolytes, serum bio markers, urine output, hemodynamic indicators, dose of intravenous epinephrine before treatment, 6, 12, 24, 48 h after treatment and at the end of CBP.Complications of CBP were also observed.Statistical analysis was performed with ANOVA and Dunnett-t test.Results The underlying problems of the ten newborns were septicemia (n=5), severe neonatal asphyxia (n=2), congenital hereditary metabolic disease (n=2) and traumatic asphyxia (n=l).The venous catheter was successfully inserted for all babies and CBP treatment continued for (86.7 ± 25.9) h averagely with obvious effect.Four of the ten cases were cured and discharged, and the rest six refused to treatment and died after due to irreversible injury of the nervous system although they had survived from the oliguric stage of ARF.The complications of CBP included thrombocytopenia (n=3), catheter blockage (n=2), hypotension (n=l).No hypothermia, thrombosis, bleeding or infection occurred.The mean blood pressure and partial pressure of oxygen in arterial blood/fraction of inspiration oxygen (PaO2/ FiO2) of the ten cases 6 h after the beginning of treatment were higher than those before [(46.4 ± 7.5) vs (36.5 ±8.3) mmHg, 1 mmHg=0.133 kPa;(210.0±62.0) vs (93.0±43.0) mmHg;t=2.647 and 6.378, both P ＜ 0.05].At the 12th hour since treatment start, the blood pH value was 7.4 ± 0.2, which was higher than that before treatment (6.9 ± 0.2, t=2.731, P ＜ 0.05), and kept in normal range.At the 24th hour, the serum levels of potassium, urea nitrogen and creatinine dropped to normal range compared to those before treatment [(4.8±2.9) vs (9.6± 3.6) mmol/L;(7.2±2.3) vs (13.6±6.3) mmol/L;(51.0± 12.0) vs (172.0±23.0) μ mol/L;t=4.571, 5.427 and 21.672, all P ＜ 0.05].Urine output increased from zero before the treatment to (0.7±0.3) ml/(kg · h) after 24 h (t=3.284, P ＜ 0.05).The maintaining dose of intravenous epinephrine decreased since 12 h after the beginning of treatment and was ceased at the 48th hour.Conclusion CBP is an effective and feasible treatment for critically ill neonates.

Objective To evaluate the safety and efficacy of transcatheter arterial embolization (TAE) of massive polycystic liver disease (PLD).Methods A total of 21 patients with symptomatic PLD were enrolled.The patients consisted of seventeen women and four men (aged 36-64 years,mean age,49 years).Transcatheter superselective embolization was performed with the mixture of N-butylcyanoacrylate (NBCA) and iodized oil.All patients underwent contrast enhanced computed tomography (CT) of the liver before TAE and at every 3 months for the first half year after TAE,and at 6-monthly intervals thereafter.Laboratory data,including routine blood tests and liver enzymes,were collected.T test was used for statistics.Results All procedures were successful without serious complications.There was no obvious improvement during the first three months.At follow-up of 6-12 months,symptoms notably improved in 18 of 21 patients,and these patients experienced further relief of the symptoms in the follow-up period.TAE failed to benefit in 3 patients,but there were no complaints of worsening of the symptoms.At follow-up CT,the total liver volume and total intra-hepatic cyst volume decreased significantly (t =6.75,7.73,P ＜0.01)compared with pre-TAE in 18 patients at 12 months after TAE.The total liver volume decreased from (8270 ± 3016) cm3 to (6120 ± 2680) cm3 and the total intra-hepatic cyst volume decreased from (7120 ±3070) cm3 to (4560±2488) cm3.Mild elevation of the liver enzymes returned to the normal range within 1 month in all patients.Conclusions It is suggested that transcatheter super selective embolization with the mixture of NBCA and iodized oil is a safe and effective treatment for PLD patients.This technique is a supplemental option for traditional therapy.