Objective To investigate the effects of hyperbaric oxygenation(HBO)therapy on serum cytokines and depression in post-stroke depression(PSD)and to study it's clinical implications.Methods Sixty patients with PSD were divided into two groups.Patients in routine treatment group(RT group)were treated with routine clinical treatment,whereas those in HBO group were treated with HBO therapy in addition to routine clinical treatment.The serum cytokines of tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)were measured by enzyme-linked immunosorbent assay(ELISA)before and after 30 days of treatment.All the patients were evaluated with Hamilton depression scale(HAMD),Chinese stroke scale(CSS)and Barthel index(BI).The evaluations were carried out at the 0 and 30 d.Results Before treatment,the concentrations of TNF-α and IL-1β in patients with PSD were significantly higher than those in patients with cerebral infarction without depression(P＜0.01),and the serum levels of TNF-α and IL-1β in those with serious depression were higher than those with moderate and mild depression(P＜0.01),and that in moderate depression patients were higher than that in mild depression patients{P＜0.01).At the 30th d post-treatment,those parameters all decreased significantly in RT group and HBO group(P＜0.01,P＜0.05).Moreover,those parameters were lower in HBO group than those in RT group(P＜0.01),and HAMD,CSS and BI scores in HBO group were significantly better than that in RT group(P＜0.05,P＜0.01).Conclusions Serum cytokines levels increased significantly in patients with PSD and were associated with the severity of depression.Serum cytokines might played an important role in pathogenesis of PSD.HBO therapy could help decrease the levels of TNF-α and IL-1β.HBO might exert therapeutic effect by reducing secondary the inflammatory injury in acute cerebral infarction.

Objective:To investigate the effects of electroacupuncture (EA) on intestinal motility disorder in rats with irritable bowel syndrome (IBS) in order to provide experimental evidence for improving clinical treatment to IBS with EA. Methods: IBS was induced by inserting a balloon into the rectum of male Sprague-Dawley rats from their neonatal age (8th, 21st day after birth) and applying colorectal distention stimulation by inflating air into the balloon. Peristaltic wave (PW) was used to estimate intestinal motility. Rats were divided into 4 groups (n=6): normal group, untreated group, sham EA group and EA group. Acupoints of Zusanli (ST36) and Shangjuxu (ST37) in the hind limbs bilaterally were chosen for EA and sham EA treatment. Trains of dense-sparse frequencies (100 Hz and 2 Hz alternately) and intensity of 1, 2 and 3 mA (10 min for each) were applied for 30 min with each EA treatment while inserting similar needles without electrical stimulation was done as sham EA treatment. PW recorded for 30 min in normal and untreated groups respectively was taken as control values, while PW recorded after EA or sham EA treatment for 30 min was taken as responsive values. Results: Frequency of PW was significantly increased in the untreated group as compared with that in the normal group (P<0.05), and decreased after 30-min EA treatment (P<0.05). There was no significant change in the PW of sham EA group as compared with the control group and before treatment (P>0.05). Conclusion: These results suggest that 1) there is an abnormal increase in intestinal motility indicating an intestinal motility disorder in IBS rats; 2) EA but not sham EA can relieve such intestinal motility disorder.

Objective To investigate the effects of nicergoline on the treatment of posterior circulation ischemia vertigo (PCIV).Methods A total of 87 patients with PCIV were randomly treated either with nicergoline (45 cases) or betahistine (42 eases).Dizziness assessment rating scale (DARS) and dizziness handicap inventory (DHI) were used to evaluate the changes of manifestations before treatment,after 7 days and 8 weeks treatment with nicergoline,meanwhile transcranial Doppler ultrasound (TCD) and brainstem auditory evoked potential (BAEP) were observed to assess the effects.Results After 7 days of treatment,the scores of DARS and DHI were decreased significantly in nicergoline group (DARS:15.6±2.1 ; DHI:38.3± 11.4) and control group (DARS:14.3±4.4; DHI:39.5±14.7) compared with the scores before treatment (DARS:24.9±7.3,22.5 ±9.7; DHI:55.8±19.7,57.5±19.3) (all P＜ 0.05),and the treatment efficiency was 71.1％ (32/45),61.9％ (26/42).After 8 weeks of treatment,the scores of DARS and DHI were also decreased significantly in nicergoline group (DARS:8.9 ±3.9; DHI:26.3± 12.8) and control group (DARS:9.9±4.7; DHI:35.8±13.1) compared with the scores before treatment (all P＜ 0.05),and the treatment efficiency was 93.3％ (42/45),78.6％ (33/42).There was significantly differences in the scores of DHI and treatment efficiency between the two groups at 8 weeks (t =2.169,P＜ 0.05,and x2=3.98,P＜ 0.05),but no differences at 7 days (both P＞ 0.05).In consistent with the remission of the clinical symptoms,nicergoline group significantly recovered from both low blood diffusion in the verbal-basal artery system and extended peak latency(PL) and inter peak latency(IPL) in the auditory pathway examined by TCD and BAEP individually at 8 weeks,especially in the upper part of the brainstem (P＜0.05).Conclusions Nicergoline is very effective in the treatment of PCIV and could alleviate the subclinical ischemia to get long time benefits.

BACKGROUND:Stem cells are a kind of cells characterized as species diversity, self-replication and renewal
ability, multiple differentiation potential and high proliferation potential. Then, the stem celltreatment for ischemic brain injury would be of great benefit. Stem celltherapy provides a new way for the treatment of ischemic stroke, but the mechanism is stil unclear.
OBJECTIVE:To describe the types of stem cells and review the mechanism underlying stem celltreatment for ischemic stroke.
METHODS:The first author retrieved PubMed database, Chinese Journal Ful-text Database for articles related to stem cellclassification and effectiveness, safety and mechanism of stem celltherapy for ischemic stroke
published from January 1992 to September 2012. The key words were“stem cells, brain ischemic stroke,
transplantation, treatment”in English and Chinese, respectively. A total of 168 literatures were retrieved, and 61 articles met the inclusion criteria.
RESULTS AND CONCLUSION:Stem celltherapy for ischemic stroke has shown a promising prospect though it is staged in the period of animal models. Stem celltransplantation for promoting functional recovery in the treatment of stroke has been completed in the clinical phase I or phase II trials. Stem celltransplantation for ischemic stroke appears to have no adverse reactions and to promote functional recovery. Main difficulties in stem celltransplantation for treatment of ischemic stroke include sources of stem cells, transplantation approach, stem cellsurvival in the host body, stem cellintegration with the host brain, therapeutic effectiveness and security. Based on the acquired results from the mechanism research and clinical trials, how to safely and quickly apply the stem celltherapy from the experiments to the clinic stil needs to work.

Objective To explore the X-ray and MRI manifestations of hip joint lesion in ankylosing spondylitis patients and give useful reference for diagnosis,assess of disease activity and therapeutic response and prognosis.Methods The X-ray manifestation and MRI manifestation of 64 cases of patients with ankylosing spondylitis were analyzed.Results In X-ray analyzed acetabulum joint changes 42,hip joint hyperostosis 47,hip joint interval narrow 13,hip joint osteoporosis 11,MRI analyzed synovial inflammatory change 64,subcartilaginous bone marrow edema 48,joint cartilage lesion and joint surface saccular change 42,tendon and ligament of enthesopathy 24,joint space change 13.Conclusion In patients with ankylosing spondylitis,X-ray manifestation of hip-joint lesion commonly included leacetabulum joint changes,hip joint hyperostosis and hip joint space narrowing while MRI manifestation included inflammation of synovium,edema of subcartilaginous bone marrow,and tendon of enthesopathy which suggested high disease activity.To suspicious hip-joint lesion in patients with ankylosing spondylitis,magnetic resonance imaging should be taken for judgment of disease activity and determine of treatment plan.

BACKGROUND:Simple medication therapy can not promote the healing of articular cartilage defects effectively;the insufficient sources of autogenous cartilage also limited cartilage transDlantation.OBJECTIVE:The types of articular cartilage injury and local micro-environment changes were analyzed to summarize the progress of tissue engineered seed cell transplantation and cell transplantation therapy for repairing articular cartilage injury.METHODS:The databases of PubMed database(http://www.ncbi.nlm.nih.gov/sites/entrez/)and CNKI(http://www.cnki.net/)were retrieved with the key words of"Tissue engineering,cell transplantation,articular cartilage defects",and the literature was limited to English and Chinese languages.Studies concerning articular cartilage injury or was closely related to tissue engineered seed cells transplantatiOn were included.Repetitive studies were excluded Survival and migration of seed cells,as well as the recovery of joint function and adverse reactions were served as evaluation index.RESULTS AND CONCLUSION:A total of 201 literatures were seized by computers,according to the inclusion criteria,papers concerning tissue engineering cells for repairing articular cartilage injury were analyzed.Clinically,articular cartilage damage was commonly resulted by traumatic arthritis,which is difficult to repair due to the poor self-repair capacity of articular cartilage.The emergence of tissue engineered cell transplantation brought new hope for articular cartilage injury healing Cell transplantation has obtained good effects in articular cartilage repair,but the technology is still room for improvement.How to technically improve the tissue-engineered 3 elements,namely,cells,scaffold materials,and biological activity factors were the research focuses.Autologous cartilage regeneration was the theoretical support for articular cartilage defects repairing,which needs to optimize its regeneration and to maintain a more stable chondrocyte phenotype in further research The tissue-engineered cells had received good effects in repairing articular cartilage injury,and it is expected to be a new clinical treatment for articular cartilage injury with the in-depth study of tissue engineering.

Objective Though X-ray measurement of metacarpal bones of right hand in children aged 7-12 in Kaschin-Beck disease (KBD) endemic areas in Xinghai County,Qinghai Province,to provide scientific basis for KBD diagnosis and research on growth and development of children in KBD endemic areas.Methods In 2012,277 children aged 7-12 were carried out clinical examination and right hand X-ray photos in KBD endemic areas,Xinghai County,Qinghai Province.Metacarpal bones were measured by electronic digital vernier caliper,and the axis length and width of the metacarpal bones and its metacarpal bone index were calculated.All data were analyzed by SPSS 19.0 software.Results The axis lengths of the metacarpal bones were in an order of 2.metacarpal ＞ 3.metacarpal ＞ 4.metacarpal ＞ 5.metacarpal ＞ 1.metacarpal in 6 different age groups and different genders; the width of 1.metacarpal bone was the widest; the width of 4.metacarpal bone was the smallest,and the rest of metacarpal bones were similar.The metacarpal bone indexes of female [(7.96 ± 0.76),(8.24 ± 0.92),(8.24 ± 0.71),(8.26 ± 0.92),(8.60 ± 1.02),(8.67 ± 0.84)mm] were higher than those of male's[(7.30 ± 0.78),(7.53 ± 0.77),(7.98 ± 0.90),(7.79 ± 0.56),(7.96 ± 0.64),(8.17 ± 0.92)mm,F =4.302,P ＜ 0.05].Conclusion The axis length,width and index of metacarpal bones of right hand in children between KBD endemic areas and no-KBD endemic areas are similar.

Objective To compare the sensitivity and specificity between indirect enzyme -linked immunosorbent assay (ELISA)and double -antibody sandwich ELISA kits produced in China and to select the best ELISA kit.Methods Samples for evaluation included 60 serum plates and 40 serum samples positive or weakly positive for antibody to hepatitis C virus (anti -HCV)which were confirmed by re-combinant immunoblot assay.These samples were tested with a sandwich ELISA kit and three indirect ELISA kits,all of which were pro-duced in China.Comparison between ELISA kits was made by paired chi -square test;comparison of false negative rate was made by R × C contingency table test.Results The sensitivities of three indirect ELISA kits and a sandwich ELISA kit were 90.2%,78.0%,95.1%, and 97.6%,respectively,and the specificities were 78.1%,72.6%,94.1%,and 100%,respectively.The sandwich ELISA kit had a 4-8 times higher sensitivity than indirect ELISA kits.The R ×C contingency table test revealed significant differences in false negative rate between ELISA kits and combinations of ELISA kits (χ2 =29.898,P <0.05).Conclusion Sandwich ELISA kit has higher sensitivity and specificity than indirect ELISA kits.Combined use of sandwich ELISA and indirect ELISA kits can significantly reduce the false negative rate and effectively prevent missed anti -HCV detection.

OBJECTIVE:To evaluate the current situation of drug utilization in inpatients with schizophrenia in our hospital. METHODS:The medication of inpatients with schizophrenia during Nov. 2007～Nov.2008 were analyzed statistically,referring to the DDD and DUI method recommended by WHO. RESULTS:Quetiapine,olanzapine,risperidone dominated the first 3 place in DDDs,which were all atypical antipsychotic drugs. Sulpiride ranked at 17th place in the list of typical antipsychotic drugs. the DUI of most drugs were lower than 1.0. CONCLUSION:The medication of most patients with schizophrenia is appropriate. Atypical antipsychotic drugs occupy a dominant position in the clinic.

AIM: To select the proper excipients for granule of compound Saliva Miltiorrhiza and the preparative process. METHODS: Building up properties, solubility and resistance to humid were used as the judgement criteria, the kinds and ratio of the excipients and preparative process sieved out. RESULTS: Properere excipent combination consisted of one portion of the extract of compound Salvia Miltiorrhiza, 1.6 times dextin, 2.6% CMC Na, 1% PVP, 2.6% CMS Na. CONCLUSION: The process and its excipent combination is better than the other process of formulation.