Seminar is an effcient teaching method in cultivation of independent thinking and research ability of residents of obstetric and gynecology.On the basis of introducing the concept of seminar,this paper discusses the application and significance of seminar in standardized training of residents.Seminar teaching can develop the capabilities of each resident to probe into and study some new things by themselves.The wide applications of this method also help to improve the abilities of the residents to understand the theoretical knowledge of gynecology and to elevate their comprehensive competence.

Objective To study the effect of an angiogenesis inhibitor TNP-470 (TNP )used alone and in combination with cytoxan(CTX) in the treatment of human ovarian cancer transplanted s.c. in nude mice. Methods Human ovarian cancer transplanted s.c. in nude mice model was established, then divided into 5 groups: control group, vehicle group, TNP group,CTX group and TNP+CTX group, different treatments were served from day 8 after transplantation and all mice were sacrificed after 28 days. The weights of the mice and the volumes of the tumors were measured respectively during the therapy time. Moreover, microscopy was done after H&E staining. Results The growth inhibiting rates in the TNP and CTX group were 26.1% and 33.9% respectively; After combined, the rate was increased to 70.5%. There were no obvious decrease in the weight of all treated mice. Conclusions Treatment with TNP is an potentially useful method of antitumor therapy in ovarian cancer, although the inhibition effects were not obvious in small doses. Moreover,TNP could enhance the effectiveness of antitumor drug.

Using ovarian cancer cell lines, we studied relationship between C-erbB2 expression and lysis activity of lymphokine-activated killer cells (LAK) and in vitro regulation by ?-interferon (IFN-?). The results showed that C-erbB2 overexpression was associated with resistance to lysis activity of LAK in ovarian cancer; IFN-? could reduce the overexpression of C-erbB2 and increase lysis activity of LAK on ovarian cancer cells in which C-erbB2 were overexpressed.

Objective:To explore coping strategies of college students during exam period. Methods:One hundred and ninety-nine subjects were randomly collected and surveyed with a self-developed questionnaire. The obtained data was analyzed with the principal component factor analysis.Results:It was found that begging sympathy or depending on fantasy, seeking support from relatives and friends, escape and abandon, teamwork and effective time management, and learning and review seriously were six common coping strategies. Students with different levels of stress reactions adopted different strategies. However, only the strategy of teamwork and effective time management was effect in stress coping.Conclusion: Teamwork and effective time management is a practical coping of students during exam period.

Objective To study the anatomical relationship between the umbilicus and the retroperitoneal major vessels. Methods A total of 80 female patients undergoing laparoscopy in our department were enrolled in this study. The patients were divided into 4 groups according to their body mass index (BMI):low-weight group,normal-weight group,overweight group,and obese group. During the operation,the surgeons palpated the abdominal aorta,right common iliac artery,and left common iliac artery with hand to determine their relationship to the umbilicus. The abdominal aortic bifurcation was used as a reference point to determine its position in relation to the umbilicus. Results (1) The bifurcation was above the level of the umbilicus in 17 (21.2%) cases,at the umbilicus level in 29 (36.3%) cases,and below that in 34 (42.5%) cases. Among the four groups,the position of the bifurcation was significantly different (?2=16.769,P=0.010). In the overweight and obese groups,most of the patients had the umbilicus located under the level of the aortic bifurcation [65.0% (13/20) and 75.0% (6/8) respectively],while in the normal-weight group,47.6% (20/42) of the cases had the umbilicus above the aortic bifurcation(P

Objective To evaluate the clinical effects of surgical treatment for diabetic retinopathy (DR) with diffuse diabetic macular edema (DDME) and massive subretinal hard exudates (MSHE) with vitrectomy, endolaser photocoagulation to the retina under perfluorocarbon liquid and long acting gas tamponade. Methods Fifteen eyes of twelve patients with DR complicated with DDME and MSHE admitted from October 2001 to December 2003 were enrolled for study, in whom 9 eyes were in 7 males and 6 eyes in 5 females. The patients were aged from 36~65 years (mean 53.4). The best corrected visual acuity (BCVA), color fundus photography and fundus fluorescein angiography were recorded, and optic coherence tomography was performed preoperatively. Pars plana vitrectomy, posterior hyaloid removal, endolaser retinal photocoagulation under perfluorocarbon liquid and long acting gas tamponade were the therapeutic modalities used to treat the diseased eyes. Results Preoperative BCVA was finger counting to 0.05 in all 15 eyes. Improved vision was observed in eyes during 4~25 months of follow up period (mean 10.26 months), 0.03~0.04 in 2 eyes(13.3%), BCVA ≥0.09 in 13 eyes(86.7%), and 1 eye(6.7%)showed an improved vision from 0.04 to 1.0. Significant decrease in macular edema and retinal thickness were observed, and marlsed to complete absorption of intra-retinal and pre-retinal hemorrhage as well as MSHE was also seen. Conclusions Vitrectomy, posterior hyaloid removal, endolaser retinal photocoagulation under perfluorocarbon liquid and long acting gas tamponade may offer satisfactory effects in the treatment for DDME complicated with MSHE.

Objective To Study The relationship between the serum markers with liver fibrosis in chronic hepatitis B and changes in liver histology. Methods The serum levels of hyaluronic acid (HA),procollagen type Ⅲ(PCⅢ),laminin(LN),typeⅣ procollagen (IV.C) and transforming growth factor ?1(TGF-?1) were measured by RIA Kit in patient with chronic hepatitis B. At the same time ,liver biopsy was performed.Results The serum levels of above five markers increased with hepatitis and fibrosis progression . The relationship between the serum levels of PC Ⅲ with hepatitis was close,both HA and TGF-?1 was closely related to fibrosis.Conclusion The combinated assay of serum levels of above five markers can identify pathological stages of hepatic fibrosis and hepatitis .

Objective To investigate the relationship between plasma neuropeptide Y(NPY) and asphyxia in newborns. Methods Thirty-seven patients with neonatal asphyxia (asphyxia group) and 33 healthy pregnant women(control group )in their third trimester were chosen. The concentrations of plasma NPY were measured by radioimmunoassay. Results The levels of NPY in umbilical artery plasma was (187.47? 46.63) ng/L in asphyxia group,and (115.33?29.42) ng/L in control group. There were significant difference between them ( P 0.05). Conclusions The neonatal asphyxia was associated with NPY. NPY might play an important role in the pathophysiological changes in neonatal asphyxia.

Objective To assess the biomechanical stability and vertebra strain distribution of asymmetrical posterior internal fixation for minimally invasive transforaminal lumbar interbody fusion ( MI-TLIF) . Methods Range of motion ( ROM) and strain distribution testing were performed in 8 fresh-frozen calf lumbar spine motion segments in flexion/extension, lateral bending, and axial rotation using 5. 0 Nm torques at the L4-5 motion segment. The sequential test configurations included intact motion segment, TLIF with unilateral pedicle screw ( UPS) , TLIF with UPS plus transfacet pedicle screws ( UPS+TFPS) , and TLIF with bilateral pedicle screw ( BPS) . The ROM was deter-mined to assess the construct stability. Strain distribution was recorded along with flexion and lateral bending configurations. Results In flexion/extension, lateral bending, and axial rotation, there was no significant difference in the ROM between BPS and UPS+TFPS fixation after TLIF. After TLIF, the UPS construct provided less segment stability than BPS and UPS+TFPS fixation in flexion, lateral bending. Strain distribution under UPS+TFPS fixation was respectively 21. 8% and 24. 2% higher than that under BPS fixation along with flexion and lateral bending. Conclusion UPS+TFPS fixation provides stability comparable to that of MI-TLIF with bilateral PS, with better load share with the vertebrae body.

To explore an effective method with few operative complications for treating circumferential mixed hemorrhoids (CMH).Sixty three cases of CMH (Group A) were treated with limited external incision and internal ligation combined with restoration of intervenient skin and 34 cases (Group B) were treated with traditional external incision and internal ligation as control. Anal canal diameter (ACD), anal canal quiescent pressure (ACQP) and anal canal systolic pressure (ACSP) were determined before and after operation. Short term and long term therapeutic effects were also observed and compared in the two groups.Short term and long term therapeutic effects in Group A were better than those in Group B (P