Research on CellSearch system's detection rate of cells and linearity performance detection method, so as to analyze the accurate and reasonable detection method to meet the CellSearch characteristics of the system.
Biomarkers, Tumor ; Cell Line, Tumor ; Cell Separation ; methods ; Humans ; Neoplastic Cells, Circulating

Objective To explore the relationship between transitional care model and diabetic patients. Methods 112 patients with type 2 diabetes mellitus were divided into observation group and control group according to random number table. Control group used the doctor as the center patient management mode. The observation group used thepatient-centeredpatient management, application of home nursing service. Results Glycosylated hemoglobin (HbA1c), triglyceride (TG), high-density lipoprotein (HGL), 2 h postprandial blood glucose (2hPBG), fasting blood glucose (FBG), body mass index (BMI) of observation group before treatment respectively were (11.2±2.4)%, (3.7±1.3) mmol/L, (0.5±0.2) mmol/L, (13.6±2.4) mmol/L, (11.8±2.9) mmol/L, (25.9±4.9) kg/m2. Patients discharged from hospital after care for 3 months respectively were (6.8±0.9)%, (1.0±0.3) mmol/L, (2.5±0.4) mmol/L, (8.9±1.5) mmol/L, (6.8 ± 2.0) mmol/L, (20.8 ± 5.8) kg/m2. There was statistically significant difference (t=3.5-6.6,P<0.05). Control group before treatment respectively were (11.9 ± 3.6) %, (3.8 ± 1.5) mmol/L, (0.6 ± 0.3) mmol/L, (13.9 ± 2.9) mmol/L, (11.6 ± 3.2) mmol/L, (25.9 ± 6.8) kg/m2,while patients discharged from hospital for 3 months respectively were (10.9±3.4)%, (3.3±0.7) mmol/L, (0.7±0.2) mmol/L, (12.8±4.2) mmol/L, (10.6± 2.6) mmol/L, (25.1 ± 6.6) kg/m2, there was no statistically significant difference (t=0.05-1.36,P>0.05). Patients discharged from hospital after 3 months, perception, diet, medication, exercise adherence score of observation group was (34.98 ± 5.67), (41.98 ± 5.00), (40.29 ± 5.60), (40.45 ± 7.21) points, which were obviously higher than (21.18±4.75) , (28.46±4.26), (21.88±4.58), (20.98±2.69) points of control group, the difference was statistically significant (t=13.96-19.03,P<0.01). Conclusions Using home nursing service mode in type 2 diabetes care is helping to improve blood sugar levels and improve patient compliance after discharge, fully improve the patients′prognosis.

Objective To analyze the relationship of endometrial pattern and thickness to fecundity in patients treated with menotropins.Methods A retrospective study analyzed the pregnancy results in 137 ovulation cycles induced by FSH with a homogeneous,a trilaminar patterns edometrium,and with the endometrial thickness

Objective To investigate the effects of homocysteinemia (HHcy) and hypertension on left ventricular re?modeling. Methods A total of 275 patients with coronary heart disease were divided into four groups including H-type hy? pertension group (n=96), non-H-type hypertension group (n=44), HHcy+non-hypertension group (n=53) and control group (n=65) based on their blood pression levels and plasma HHcy levels. The serum levels of glucose (Glu), total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL) and high-density lipoprotein (HDL) were compared between groups. The left ventricular end systolic diameter (LVESD), left ventricular end diastolic diameter (LVEDD), left ventricular posterior wall thickness (LVPWT), ventricular septal thickness (IVST) and left ventricular mass index (LVMI) were observed in four groups. The proportion of patients with left ventricular remodeling was also compared between four groups. The influence fac?tors of left ventricular remodeling were analysed. Results There were no significant differences in biochemical parameters except Hcy level between frour groups. The values of LVMI, left ventricular wall thickness and the proportion of patients with left ventricular remodeling were significantly higher in H-type hypertension group than those of other three groups ( P<0.05). The Hcy level was positively correlated with LVMI and left ventricular wall thickness. Logistic regression analysis showed that HHcy and hypertension were the risk factors of left ventricular remodeling (OR=7.443, 7.754 and 9.948,P<0.05). The risk factors of left ventricular remodeling were higher in patients with both HHcy and hypertension than those in patients with HHcy or hypertension. Conclusion Homocysteine and higher systolic pressure are independent risk factors for left ventricular remodeling and they have a synergistic effect on leading to left ventricular remodeling.

In terms of performance of piezoelectric micro pump, this paper explores the piezoelectric ceramic plate in different wave driven micro pump flow rate. The conclusion is that the square wave voltage gets the biggest micro pump velocity. The velocity and pressure of the micro pump is almost linear relationship, and having nothing to do with the different inner diameter pipes. The piezoelectric micro pump's stability is not good and exists attenuation.
Ceramics ; Insulin ; administration & dosage ; Insulin Infusion Systems

Objective To study the effects of density,phosphorous(P),and nitrogen(N)fertilizers on yield and quality of Andrographis paniculata.Methods Randomized block design in yield was used and HPLC was used to analyze the content of andrographolide and dehydroandrographolide.Results Plant height,fresh weight,dry weight,content of dehydroandrographolide were influenced significantly by density.The quality character and economic yield of content of andrographolide,dehydroandrographolide,total content of both andrographolide and dehydroandrographolide were significantly influenced by P nutrition.The economic yield of dry weight,content of andrographolide and dehydroandrographolide,and total content of both andrographolide and dehydroandrographolide were influenced significantly or extreme significantly by N nutrition.P and the interaction of P and N fertilizer extremely influenced the economic yield.The relative influences between plant weight and dry weight was extremely significance,that between quality character and quality character,that between quality character and economic yield were significant or extremely significant.Conclusion When planted with 1.8?105 /hm2,fertilizered with P2O5 540 kg/hm2 and pure N 225 kg/hm2,the hightest economic yield is gained.

Objective To explore the serum levels of growth differentiation factor-15 (GDF-15) in patients with acute exacerbation of chronic heart failure (CHF) and its correlation with other common indexes,to provide reference for its clinical diagnosis,treatment and prognosis.Methods Two hundred patients with acute exacerbation of CHF were selected as CHF group,and 100 matched healthy volunteers were selected as control group.Serum levels of GDF-15 and N-terminal pronatriuretic peptide (NT-proBNP) were detected,and left ventricular end diastolic diameter (LVESD),left ventricular end diastolic diameter (LVEDD),and left ventricular ejection fraction (LVEF) were measured by echocardiography within 2 hours after admitted to hospital,and after the symptoms improved of CHF group,and on health examination day of control group.Patients in CHF group were followed up to record CHF related adverse events.Correlations between GDF-15 and other indicators were analyzed by Spearman correlation analysis,and the clinical value of serum GDF-15 on diagnosing CHF was analyzed by the receiver operating characteristic curve (ROC) and the area under the curve (AUC).Results The serum levels of GDF-15 and NT-proBNP in each time-point of CHF group were all higher than those of control group (t =4.70 ～ 7.11,P ＜ 0.05 orP ＜ 0.01).The serum levels of GDF-15 and NT-proBNP had negative correlation with LVEF (r =-0.539,-0.572,P ＜ 0.01),and had positive correlation with LVESD,LVEDD,and NYHA cardiac functional grading (r =0.505 ～ 0.861,P ＜ 0.01).Serum GDF-15 had positive correlation with serum NT-proBNP (r =0.528,P ＜0.01).With the increase of serum GDF-15 level,CHF group's readmission (rate) and death (rate) were both increased (x2 =36.86,26.59,P ＜0.01).AUC of predicting readmission risk by serum GDF-15 was 0.822 (95％ CI:0.719 ～0.890,P ＜0.01),and the best predictive cutoff point was 2 876.30 ng/L (sensitivity was 91.86％,specificity was 73.27％).AUC of predicting mortality risk was 0.816 (95％ CI:0.715 ～ 0.885,P ＜ 0.01),and the best predictive cutoff point was 3 487.05 ng/L (sensitivity was 91.72％,specificity was 69.05％).Conclusions Serum GDF-15 level in patients with acute exacerbation of CHF is higher,decreases with symptoms improvement,has positive correlation with LVESD,LVEDD,and NYHA cardiac functional grading,and has negative correlation with LVEF,has higher sensitivity on predicting CHF-related adverse events,and the mechanism may be related to the activation of SMAD pathway.Therefore,it may be a promising biomarker for clinical diagnosis and prognosis of cardiovascular diseases.

Objective: To optimize the alcohol-extraction process and condition of Radix Pulsatillae.Methods: Alcohol concentration,the extraction time and number of extraction on effect of extraction rate of pulchinenoside B4 were evaluated by orthogonal design L9(34).Results: The optimum condition of the alcohol extraction was: 75% ethanol,3 times of extraction,3h for extraction process.Conclusion: The extraction method can be used for reference for the extraction process of Radix Pulsatillae.

Objective To analyze the genotype distribution of human papillomavirus( HPV) strains and their epidemiological characteristics in cervixes of Chinese females. Methods Pertinent litera-tures published during 2004 to 2013 were screened from PubMed,China National Knowledge Infrastructure (CNKI)and Wanfang database for data analysis. Results There were 245 studies screened out for the me-ta-analysis. A total of 661 658 cases met the inclusion criteria,ranging from 16 to 87 years old. All prov-inces and territories of China were covered by the data. The overall rate of HPV infection in cervix was 25. 0% among Chinese females. The predominant high risk genotype of human papillomavirus strains was HPV16,followed by HPV52,HPV58,HPV18,HPV33 and HPV31. HPV52 and HPV58 genotypes were more prevalent than HPV18 genotype. HPV35 and HPV45 genotypes were less popular. Conclusion The study suggested that the HPV infection showed obvious regional differences in genotypes. The genotype dis-tribution of HPV infection in China was different from that in other regions of the world. There were differ-ences with genotype distribution of HPV strains among seven geographic regions of China.

Objective To explore the value of serum SC5b-9, anti-C1q antibody, C3 and C4 levels in the assessment of lupus activity. Methods The enzyme linked immunosorbent assay (ELISA) was used to measure SC5b-9 and anti-C1q antibody, rate nepheiometry was used to detect the serum level of C3 and C4 in sera of 62 SLE patients, 35 patients with other rheumatic diseases (including rheumatoid arthritis, ankylosing spondylitis, primary Sjogren' s syndrome, mixed connective tissue disease, dermatomyositis, polymyositis, systemic sclerosis and vasculitis) and 35 healthy controls. And the correlation between above-mentioned parameters and lupus clinical manifestations, disease activity and histological type of lupus nephritis were analyzed. Results In SLE patients, the levels of SC5b-9 and anti-C1q antibody were significantly higher than those in patients with other rheumatic diseases and healthy controls (P<0.05). The titers of SC5b-9 and anti-C1q antibody negatively correlated with C3 and C4 (P<0.05), and positively correlated with SLEDAI (P<0.05). The sensitivity and specificity of the combination of these three measurements for SLE was 95.37% and 98.46 respectively. SC5b-9 and anti-C1q antibody were associated with the presence of proliferative glomerulonephritis (P <0.05). Conclusion Taking the evaluation of all these three measurements simultaneously is valuable for the diagnosis of lupus flare. SC5b-9 and anti-C1q antibody may play major roles in the immunopathogenesis of lupus nephritis.