stir-baked Radix Paeoniae Alba with vinegar. Conclusion Different processing method has great influence on the paeoniflorin content of Radix Paeoniae Alba.

Objective To study the effects of density,phosphorous(P),and nitrogen(N)fertilizers on yield and quality of Andrographis paniculata.Methods Randomized block design in yield was used and HPLC was used to analyze the content of andrographolide and dehydroandrographolide.Results Plant height,fresh weight,dry weight,content of dehydroandrographolide were influenced significantly by density.The quality character and economic yield of content of andrographolide,dehydroandrographolide,total content of both andrographolide and dehydroandrographolide were significantly influenced by P nutrition.The economic yield of dry weight,content of andrographolide and dehydroandrographolide,and total content of both andrographolide and dehydroandrographolide were influenced significantly or extreme significantly by N nutrition.P and the interaction of P and N fertilizer extremely influenced the economic yield.The relative influences between plant weight and dry weight was extremely significance,that between quality character and quality character,that between quality character and economic yield were significant or extremely significant.Conclusion When planted with 1.8?105 /hm2,fertilizered with P2O5 540 kg/hm2 and pure N 225 kg/hm2,the hightest economic yield is gained.

Objective: To optimize the alcohol-extraction process and condition of Radix Pulsatillae.Methods: Alcohol concentration,the extraction time and number of extraction on effect of extraction rate of pulchinenoside B4 were evaluated by orthogonal design L9(34).Results: The optimum condition of the alcohol extraction was: 75% ethanol,3 times of extraction,3h for extraction process.Conclusion: The extraction method can be used for reference for the extraction process of Radix Pulsatillae.

Objective To explore the effect of hepatitis B virus (HBV) X protein (HBx) on autotaxin (ATX) expression and its significance. Methods The recombinant eukaryotic expression vector of HBx ,pcD-NA3.1(+)-HBx,and the recombinant luciferase reporter gene vector of ATX promoter,pGL3-ATX,were con-structed and used to co-transfect HepG2 cells to examine the effect of HBx on the activity of ATX promoter. The sta-ble cell expressing HBx,HepG2.HBx,was constructed,and Western blot(WB)was used to detect the effect of HBx on ATX expression. Results The luciferase activity of pcDNA3.1(+)-HBx and pGL3-ATX group was 1.47 times as that of the empty vector cDNA3.1(+)and pGL3-ATX group(P<0.000). WB detection showed that the expression of ATX protein was increased in HepG2.HBx cells,and 1.75 times as that of HepG2 cells(P<0.05). Conclusion HBx can activate ATX promoter and up-regulate ATX expression ,thus suggests that HBV infection might enhance ATX/LPA signaling.