Non-suicidal self-injury is common in adolescents. At present, the pathological mechanism of non-suicidal self-injury is still unclear, and there is a lack of objective biological markers in diagnosis and treatment, which is an urgent problem to be solved in clinical diagnosis and treatment. The magnetic resonance imaging is an important technique to explore the imaging mechanism of non-suicidal self-injury. The purpose of this review is to systematic evaluation of the latest research results of magnetic resonance imaging of non-suicidal self-injury. It was found that non-suicidal self-injury in people without other mental disorders showed abnormal damage in the orbitofrontal, the dorsolateral prefrontal lobe, the medial prefrontal lobe, the ventrolateral prefrontal lobe, the amygdala, the cingulate gyrus, the supramarginal gyrus, the amygdala, the hippocampus, the insular, the corpus callosum, the thalamus, the putamen, the dorsal striatum, the cuneate prefrontal lobe and the right temporal lobe. These areas are the core areas related to emotional processing, decision-making, cognition and movement. Non-suicidal self-injury with other mental disorders such as borderline personality disorder and depression may be affected by underlying diseases and exhibit different damage patterns, which showing abnormal brain regions related to emotional network, decision-making, social cognition and exercise.The results of this review can be helpful for the future study of the magnetic resonance imaging mechanism of non-suicidal self-injury.

Objective To explore the methods in repairing the thenar wounds by microsurgical technique Methods Since 1989, thenar wounds were restored with transfer of superficial or deep palmar arch, recombine of thenar muscle flap Patch of nerve and transplantation of flap on 21 cases Results The thumb and flap were survived The two point discrimination was 5 to 10 mm The thenar muscle streugth reached MRC 4 with satisfactory oppones fuction Conclusion The thenal wounds cna be obtained good effect being repaired by microsurgical technique

Objective To study the percentage and the distribution of nosocomial infection, and susceptibility of related pathogens to antibiotics in patients with systemic lupus erythematosus (SLE). Methods The clinical data of in patients with SLE in our hospital from June, 1990 to June, 1998 were analyzed. The susceptibility of the pathogens to antimicrobial agents was tested by K-B paper dilution method. Results The percentage of nosocomial infection in these cases was 16.1％ . The major pathogens were staphylococcas aureus, Escherichia coli and Candida. The most highly resistant microbes were E.coli, S.arueus and Pseudomonas aeruginosa. Conclusions The nosocomial infection is one of the most common complication of SLE in the in patients. It is necessary to examine the pathogens regularly and use antibacterial agents according to the susceptibility.

BACKGROUND:Health has been paid more and more attention in modern society, the health of those special groups, such as the elderly and the disabled, should also be brought to the forefront. As fitness equipment is more and more popular in contemporary, our focus should be transferred to the use of fitness equipment for these special groups.
OBJECTIVE:To put forward the design strategy of fitness equipment control interface, for the special groups to use common fitness equipment comfortably and conveniently.
METHODS:Based on the research of existing fitness equipment control interface, according to the usage habits and cognitive awareness of special groups on control interface, a new design strategy has been put forward using the general design method and ergonomics.
RESULTS AND CONCLUSION:Based on the existing 45 kinds of exercise bikes, 84 kinds of running machines and 129 kinds of treadmil fitness equipments, we found that these equipments are designed for common users in the aspects of design and production process and control interface. Universal design is a design concept beyond the lack of capacity to meet the different needs of different populations, and designed products can reduce or even eliminate the difference in the ability of special populations and the general population in use. The new strategy makes design of fitness equipment control interface generalized and good man-machine interactive, which can meet the demand of the special groups and help them do fitness exercise to improve their health and body function.

BRAF is one of the most important pro-oncogenes, which is mutated in approximately 8% of human tumors. The most common BRAF mutation is a valine-to-glutamate transition (V600E) that is expressed primarily in melanoma, colorectal cancer and thyroid carcinoma. MEK/ERK is constitutively activated in the cells expressing BRAFV600E, leading to tumor development, invasion, and metastasis. Therefore, BRAFV600E is a therapeutic target for melanoma and some other BRAFV600E tumors. Vemurafenib, a BRAFV600E inhibitor, which was approved by FDA for the treatment of late-stage melanoma in 2011, produces improved rates of overall and progression-free survival in patients with the BRAFV600E mutation, making a dramatic breakthrough in melanoma treatment. Vemurafenib is also an individual target drug based on genetic diagnosis. However, its therapeutic success is limited by the emergence of drug resistance. Therefore, it is important to explore the mechanisms underlying the resistance for developing new inhibitor drugs and for preventing or delaying the resistance evolution to BRAF inhibitor drugs. In this review, we described the role of BRAFV600E as an anti-tumor drug target and the development of BRAF inhibitors. We also discussed the mechanisms leading to resistance of BRAFV600E inhibitors. Furthermore, therapeutic strategies that might be employed to overcome acquired resistance were proposed.

Objective: To express CTX-M-38 type extended-spectrum-lactamase, and detect its distribution and antibiotic susceptibilities. Methods: Total of 46 strains producing ESBL E.coli was collected from the first affiliated hospital of Zheng zhou University. The CTX-M-38 ESBL gene was selected by PCR using gene recombination technique to construct pET28a-CTX-M-38. The expression of CTX-M-38 in BL21 E.coli and its antibiotic susceptibilities were carried out by liquid dilution test. Testing the enzyme activities of culture supernatant and bacteria sonicate to reflect its distribution. Results: The size of amplified gene product was about 900 bp. The DNA sequence was matched with the information of gene bank. The enzyme activities from bacteria sonicate were stronger than the culture supernatant .The transformant was resistance to penicillins, the first, second and third generations of cephalosporins. It was sensitive to imipenem. The transformant was also sensitive to ceftazidime and aztreonam in vitro, and resistance to antibiotics including beta-lactamase inhibitors except piperacillin/tazobactam. The transformant was also resistance to gentamicin,minocycline, ciprofloxacin and levofloxacin. Conclusion: The CTX-M-38 type ESBL is successfully expressed at designed experimental condition in this study. The product mainly lies inside the bacteria. The transformant shows wide resistance to antibiotics.

Objective To explore the characteristic differences of the fractional amplitude of low-frequency fluctuation (fALFF) feature of the spoutaneous neural activity between young male unipolar depression and bipolar depression patients,and determine the biological markers to distinguish the two disorders.Methods Twelve male unipolar depression,12 bipolar depression patients and 11 age and educated-matched healthy males underwent resting-state functional magnetic resonance imaging scanning at 3.0 Tesla.The whole brain' s fALFF were calculated and analyzed.Results The differences of the fALFF of the three groups had significant differences (P＜0.01,Alphasim) in the right orbital medial frontal gyrus (6,33,-9;K =29),the left medial frontal gyrus (-6,60,3;K =44) and the left paracentral lobule (-3,-27,5 1;K =20).The unipolar depression subjects had significantly higher fALFF compared with heahhy controls in the left anterior cingulate gyrus.The bipolar depression subjects had significantly higher fALFF compared with healthy controls in the bilateral medial frontal gyrus and the left middle cingulate gyrus.And the unipolar depression subjects had significantly lower fALFF compared with bipolar depression ones in the right orbital medial frontal gyrus,the right anterior cingulate gyrus and the bilateral medial frontal ~rus(all P＜0.05).Conclusions Abnormalities exist in the brain regions in male with unipolar or bipolar depression patients in the resting state,and the abnormal regions are different.

OBJECTIVE To investigate the conditions and associated risk factors of nosocomial infections caused by Stenotrophomonas maltophilia.METHODS Thirty isolates of S.maltophilia causing nosocomial infections were collected and identified with API 20NE test strips.Minimal inhibitory concentration(MIC) of 14 antimicrobial agents against 30 isolates was determined by broth microdilution method.Case-control study and multivariate Logistic regression analysis were used for statistics to verify risk factors of infections caused by S.maltophilia.RESULTS Thirty isolates of S.maltophilia were highly resistant to imipenem,meropenem,cefotaxime, aztreonam and amikacin,but showed certain susceptibility to cefoperazone/sulbactam,piperacillin/tazobactam,trimethoprim-sulfamethoxazole and ticarcillin/clavulanic acid(96.7%,76.7%,73.3% and 60.0%,respectively).The independent risk factors leading to infections of S.maltophilia were mechanical ventilation(OR=7.629) and over 60 days of length of stay(OR=4.466).CONCLUSIONS S.maltophilia shows multiresistance to commonly used antimicrobial agents.The mechanical ventilation and over 60 days of length of stay are the independant risk factors for nosocomial infections caused by S.maltophilia.

Objective To explore the characteristics of endothelial progenitor cells (EPCs) on different scaffolds and to find a new bio-engineered synthetic hybrid scaffold for artificial bio-engineered blood vessels. Methods EPCs were induced from mesenchymal stem cells isolated from rat bone marrow and seeded on ECM scaffold. The surface structure of the scaffold and growth status of EPCs on the scaffold were observed and analysed by electron microscopy. The characteristics and number of those EPCs on different kinds of scaffolds were studied with EPC-specific VWF by immunofluorescence, Western blotting and real-time PCR technique at different time points. Results The cell adhesion rate at 1,3,5 h after seeded on pressed scaffold were higher than that on unpressed scaffolds( P < 0. 01 ). Pressed scaffolds has got a larger cell number( P < 0. 05 )at DIV1, DIV3, DIV7, but there was no significant difference after DIV10. Furthermore, cell shapes of EPCs on pressed scaffolds were more mature and more similar to endothelial cells. A level cell surface on pressed scaffolds was achieved. Western blotting assays revealed EPCs on pressed scaffolds expressed more protein VWF at DIV3, DIV7, DIV10. Real-time PCR results showed EPCs on the two different groups of scaffolds all expressed VWF gene, The quantity of their expression in the two groups were all enhanced after DIV7 (P < 0. 05 ). The quantity of VWF gene expression in the pressed group was much higher than that in the unpressed group at DIV3 ,DIV7,DIV10 (P <0, 01), but there was no significant difference after DIV14. Conclusions Pressed ECM scaffolds can promote adhesion, proliferation and differentiation on EPCs. Pressed scaffolds can be used as the matrix for EPC and fabricated into a novel synthetic tissue bio-engineered vascular scaffold.

OBJECTIVE:To investigate the effect of arsenic trioxide(As2O3)on human hepatoma cells SMMC-7721 viability and the expression of urokinase plasminogen activator receptor(uPAR).METHODS:SMMC-7721 cells were cultured and treated with As2O3 at different concentrations for different time with untreated SMMC-7721 cells(without As2O3 treatment)served as control.The proliferation rate of the SMMC-7721 cells was measured by MTT assay;the change of cell cycle was detected by flow cytometry analysis;the effect of As2O3 on uPAR mRNA levels was analyzed by real-time fluorescent quantitative polymerase chain reaction.RESULTS:As2O3 had significant inhibitory effect on SMMC-7721 proliferation in time-dependent and concentration-dependent manner.As compared with control group,As2O3 significantly decreased the proportion of cells in G0/G1 phase but increased the cell proportion in G2/M phase,and it markedly down-regulated the level of uPAR mRNA expression(P