The quail model of hyperuricemia combined with abdominal obesity was induced by high purine diet.Body weight in model group showed no change.Serum uric acid level in model group was increased significantly on 7,14,21,and 28 d( P＜0.05 or P＜0.01 ).Abdominal fat index in model group increased significantly on 28d.On 7 d and 28 d,serum free fatty acid level was increased significantly.Acetyl-CoA carboxylase( ACC ) protein expression in the liver of model quail was increased as shown by ELISA and immunohistochemisty ( P＜0.05 or P＜0.01 ),suggesting that the alteration of ACC expression contributes to the pathogenesis of hyperuricemia combined with abdominal obesity.

Objective To reconstruct caudate nucleus,lentiform nucleus and thalamus three-dimensionally,fuse these neural structures with (patient's) radiological images and provide anatomical data for diagnosis and treatment of neurosurgical diseases. Methods Chinese Virtual Human dataset was segmented using image-transparency method at first and each image was smoothed by eroding and dilating, then fused neural structures with MRI,the neural structures and brain in MRI were reconstructed and displayed using surface rendering and volume rendering lastly. Results The reconstructed neural structures were smooth,natural and realistic.Their shapes and positions was clearly displayed and could be rotated, observed in any direction.On the fused images,the neural structures and tumor could be seen at the same time.Conclusion The reconstructed structures and fused images have great reference value to teaching and learning and diagnosis and treatment of neurosurgical diseases.

0.05). The NAA/Cr and NAA/Cho in the Group A were significantly higher than those in the Group B (t=4.235, P=0.011; t=2.832, P=0.031), and the Cho/Cr in the Group A was significantly lower than that in the Group B (t=-2.323, P=0.042). Conclusions ~1H magnetic resonance spectroscopy can improve the diagnostic accuracy of glioma.

Objective To study the optimal time of MRI follow-up after transsphenoidal surgery in patients with nonfunctional pituitary macroadenoma. Methods MRI records of 50 patients before surgery,within 1 postoperative week (early stage),at 3 months (intermediate stage) and 1 year after surgery (late stage),respectively,were retrospectively analyzed.Dynamic changes in the sella turcica were observed and the degrees of tumor excision were studied before and after MRI T 1-weighted enhanced scans at different postoperative stages. Results In the early stage MRI images showed that the size of sellar contents decreased by 8%～32%,tumor disappeared in 22 cases,and suspected residual tumor was found in 28 cases.At 3 months after surgery,MRI scans revealed that sellar contents decreased by 11%～85% in size.On coronal position MRI scans,the size of sellar contents decreased more than 50% in 11 cases,30%～50% in 9 cases,less than 30% in 8 cases.Out of the 28 cases that were suspected of residual tumor in the early stage,confirmation of residual tumor in the sella turcica was made in 23 cases.MRI findings 1 year after surgery showed no change in the sella turcica in 46 cases and continuing to decrease in 4 cases. Conclusions Re-examination of MRI in intermediate stage after transsphenoidal surgery in patients with nonfunctional pituitary macroadenoma facilitates the detection of residual tumor and recurrence.

Objective To evaluate the role of radiotherapy in the management of dermatofibrosarcoma protuberans. Methods Between 1995 and 1999, 18 patients with pathologically confirmed dermatofibrosarcoma protuberans were retrospectively analyzed. Nine patients were referred to our hospital for primary treatment and the remaining 9 patients came with recurrent disease. Fifteen of 18 patients received surgery followed by radiotherapy. Among them, 14 patients underwent complete resection and 1 partial resection. Majority of patients received 50～60 ?Gy. One patient received 37.5 ?Gy because he refrained from further treatment. Two patients received 52 ?Gy and 50 ?Gy due to unresectable extensive lesion. One of 18 patients was treated by total resection alone.Results The median of follow up time was 26 months with a range of 0.5～5 years. Sixteen patients (8.9%) are disease free. One patient developed local recurrence and one died of malignant progression. These two patients were treated with radiotherapy alone or surgery only. The local control rates were 100% (15/15) for patients who were treated with surgery and radiotherapy. Conclusions Postoperative radiotherapy is able to decrease the local recurrence rate in dermatofibrosarcoma protuberans.

The personnel training is one of the core tasks of clinical Chinese pharmacy.Since the professional training of clinical Chinese pharmacy has started relatively late in China,the teaching contents and curriculum still need further improvements.This paper is aiming at analyzing the post tasks of personnel of clinical Chinese pharmacy,defining the knowledge structure of them,and learning from the characteristics of clinical Chinese pharmacy teaching systems of the foreign and domestic universities.On such basis,the paper proposes the ideas about constructing the curriculum system of clinical Chinese pharmacy,to provide a reference for the higher educational personnel training of clinical Chinese pharmacy.

Objective To study the effect of stromal cell-derived factor-1(SDF-1)/CXCR4 on the proliferation of CD34+ hematopoietic stem/progenitor cells(HSPCs) supported by bone marrow mesenchymal stem cells(MSCs).Methods Rat bone marrow MSCs were plated as feeder layer in long-term cultures(LTC) with bone marrow CD34+ cells in vitro.Cultures were weekly supplemented with SDF-1,anti-SDF-1 antibody,or anti-CXCR4 antibody separately for 5 weeks.The hematopoietic-supporting activity was evaluated by the number of CD34+ cells and colony forming cell(CFC).To assess the effect of SDF-1/CXCR4 on CD34+ cell proliferation cycle,killing assays were carried out and proliferation indexes were calculated.Expression of SDF-1 and CXCR4 in MSCs and CD34+ cells were determined by flow cytometry.SDF-1 in MSC-and CD34+ cell-conditioned medium was also measured by ELISA.Results The number of CD34+ cells,CFC and proliferation indexes were significantly increased in treat-ment with SDF-1(P

Background:In the treatment of many gastric diseases,eradication of Helicobacter pylori( Hp)is important. With the increasing in antibiotic resistance of Hp,the eradication efficacy of conventional triple and quadruple therapy for Hp is decreasing in recent years. Aims:To assess the efficacy and safety of quadruple therapy combined with astragalus membranaceus for Hp eradication. Methods:A total of 395 Hp-infected patients with chronic gastritis/dyspepsia and peptic ulcer diagnosed by gastroscopy were enrolled,of them 185 patients were of chronic gastritis/dyspepsia group and 210 patients were of peptic ulcer group. Patients in the two groups were subdivided into trial group and control group. Patients in trial group were given quadruple therapy combined with astragalus membranaceus for 14 days,and patients in control group were given quadruple therapy only for 14 days. Hp eradication was assessed by 13 C-urea breath test 4 weeks after treatment. Results:A total of 358 patients(90. 6%)completed the study. In chronic gastritis/dyspepsia group,the eradication rate was significantly higher in trial group than in control group by PP analysis(P<0. 05),but no significant difference was found by ITT analysis(P>0. 05). In peptic ulcer group,the eradication rates by PP and ITT analysis showed no significant differences between trial group and control group(P>0. 05). In control group,the eradication rates by PP and ITT analysis were significantly higher in peptic ulcer group than in chronic gastritis/dyspepsia group( P <0. 01). In trial group,the eradication rates by PP and ITT analysis were not significantly different between chronic gastritis/dyspepsia group and peptic ulcer group( P >0. 05 ). The incidence of adverse effects was not significantly different between chronic gastritis/dyspepsia group and peptic ulcer group as well as trial group and control group( P>0. 05). Conclusions:Quadruple therapy combined with astragalus membranaceus could increase the eradication rate of Hp in chronic gastritis/dyspepsia group safely.

[ ABSTRACT] AIM:To explore the effect of renal transporter glucose transporter 9 ( Glu9 ) on hyperuricemia in the rats induced by fructose.METHODS:SD male rats (n=30) were randomly divided into normal group, model group and benzbromarone group , according to the weight .The rats in normal group was given water , while the rats in model group and benzbromarone group were given 10%fructose solution to establish hyperuricemia model .At the same time , the rats in normal group and model group were given a gavage of distilled water , while the rats in benzbromarone group were given benzbromarone at the dose of 20 mg/kg.The rats were sacrificed on the 40th day.The serum uric acid (SUA) and urinary uric acid (UUA) were detected to calculate the clearance rate of uric acid (CUA) in the kidney.The activity of hepatic xanthine oxidase ( XOD) was also measured .The expression of renal Glut 9 at mRNA and protein levels was determined by RT-qPCR and immunohistochemical staining .RESULTS:From the 20th day to the 40th day, the SUA in model group was significantly higher than that in normal group , but the UUA and CUA had no difference .On the 20th day, the SUA in benzbromarone group was markedly decreased as compared with model group , but UUA and CUA had no significant differ-ence.On the 40th day, the hepatic XOD activity in model group was significantly elevated , and no difference of XOD be-tween model group and the benzbromarone group was observed .Compared with normal group , the protein expression of Glut9 in the renal tissues of model group were markedly increased , and that in benzbromarone group was significantly lower than that in model group .However, no difference of the Glut9 mRNA expression was observed among groups .CONCLU-SION:Fructose drinking induces hyperuricemia in rats , which is probably related to the up-regulation of renal Glut9 ex-pression at protein level , and the increase in the reabsorption of uric acid in the kidneys .

Objective To investigate the relationship between resting heart rate(RHR) and prognosis of acute coronary syndromes without ST-segment elevation.Methods 167 patients of ACS without ST-segment elevation admitted to hospitals consecutively from Oct. 2001 to Oct. 2004 were divided into five groups according to the levels of RHR[RHR 1 group:RHR