BACKGROUND:Although tumor stem cel s and their differentiated vascular cel s, which are not sensitive to chemotherapy and molecular targeted therapy, reduce overal blood supply of the tumor, these cel s facilitate the invasion and metastasis of the tumor, eventual y resulting in treatment failure. OBJECTIVE:To investigate the effect of gastric cancer stem cel s in stomach cancer invasion and metastasis as wel as its mechanism in angiogenesis. METHODS:A total of 20 gastric cancer stem cel s and 20 normal gastric cancer cel s SGC7901 were cultured in the medium with DMEM/F12 (1:1), 2%B27 and 20μg/L epidermal growth factors or cultured in 10%PBS, respectively. Invasion of gastric cancer stem cel s was detected by sphere formation assay, colony formation assay and Transwel invasion assay. Levels of mRNA and protein expression of CD44 and E-cadherin were measured to observe tumor metastasis. Besides, angiogenesis was observed by cel scratch test, Transwel migration assay and ring test. RESULTS AND CONCLUSION:Gastric cancer cel s adhered to the medium wal , and gastric cancer stem cel s suspended in the medium, which appeared to be spindle interstitial form. Number of gastric cancer tumor spheres was significantly lower than that of gastric cancer stem cel spheres in the sphere formation assay and colony formation assay (P<0.05). Number of gastric cancer stem cel s through the basement membrane was significantly higher than that of gastric cancer cel s (P<0.05). And the cel migration distance and ring number were significantly increased in the gastric cancer stem cel s as compared with the gastric cancer cel s (P<0.05). In conclusion, the invasion, migration and angiogenesis abilities of gastric cancer stem cel s are stronger than those of gastric cancer cel s, which can further promote the occurrence and progression of gastric cancer.

Objective This study is to examine the effect of different breathing patterns (abdominal respiration,natural respiration)on skin temperature(TEMP),skin conductivity(SC) and heart rate variability (HRV) in college students.Methods We use single blind trial in the study,and randomly select thirty students,including fourteen male and sixteen female,aged 19.6±0.77 years,and then randomly divide them into two groups:the intervention group (abdominal respiration group,6 males and 9 females) and the control group(natural respiration group,8 males and 7 females).In the study three phases are included:baseline phase(five minutes),test phase(fifteen minutes),recovery phase(five minutes).Data of above indicators was collected at 3-5 min,14.5-19.5 min,and 23-25 min,respectively,during the study.Results (1) There are no significant difierences of all three indicators between the two groups in the baseline phase(P<0.05).(2)The SC of the test phase in the intervention group is lower than in the control group (P=0.05),while the TEMP and HRV of the two groups show no significant differences(P>0.05).(3)There are no significant differences of TEMP and the HRV in the recovery phase between the two groups(P>0.05),except the SC (P<0.05).Conclusion No significant difference is observed between the influences of abdominal and natural respiration on the emotion-related biological indicators.

Objective To identify and purify the allergens of Tyrophagus putrescentiae. Methods Tyrophagus putrescentiae extract was analyzed by SDS-PAGE and Western blotting, and was partially purified by DE52 anion-exchange chromatography and HiLoad 16, 60 Superdex 200 prep grade Size-exclusion chromatography. Results 23 bands were found after SDS-PAGE with comparative molecular weight (Mr) of 177 000, 118 000, 107 000, 70 000, 67 000, 60 000, 52 000, 45 000, 41 000, 40 000, 38 000, 37 000, 35 000, 27 000, 23 000, 22 000, 18 000, 17 000, 16 000, 15 000, 14 000, 13 00 and 12 000. Five allergens were detected by Western blotting with Mr 128 000, 67 000-70 000, 36 000-37 000, 18 000 and 16 000, respectively. The positive reaction rate of 3 allergens, with Mr 128 000, 67 000-70 000 and 36 000-37 000, were 100%, while that of other 2 allergens with Mr 18 000 and 16 000 was 77.8% and 44.4% respectively。The allergen with Mr 18 000 was purified by anion-exchange chromatography and Size-exclusion chromatography. Conclusion Four major allergens and one minor allergen from Tyrophagus putrescentiae have been identified.

Background Prostaglandin eye drops is front-line medicines of lowing intraocular pressure for primary open angle glaucoma (POAG),but its price is higher for long-term application.Domestic latanoprost drops has been applied in clinic.However,its trial of lowing intraocular pressure effect and safety from sizable sample and multi-center is lack.Objective This study was to observe the efficacy and safety of domestic latanoprost for POAG and ocular hypertension.Methods This prospective,multicenter trial was conducted at 25 hospitals in 5 territories of China,and 837 eyes of 576 patients with POAG and ocular hypertension were recruited in the hospitals based on the uniform criteria.Domestic latanoprost drops was topically used once per day at night for 3 months.Intraocular pressure (IOP) was measured 1 month and 2 months,3 months to assess the efficacy of drug,and vision acuity,blood pressure,pulse,ocular symptoms and signs were observed to evaluate the safety of the drug.This study approved by the Ethics Committee of Wuhan General Hospital of Guangzhou Military Command,and all patients were informed consent and signed informed consent.Results IOP was (28.36±7.86),(19.66±5.46),(19.59±7.86) and (19.35 ± 5.03) mmHg in pretreatment and posttreatment 1 month,2 months and 3 months,respectively,showing a significant difference among various time points (F =748.325,P =0.000),and the IOPs were markedly lower in posttreatment than those in pretreatment (all at P＜0.001).The falling range of IOP was (30.43 ±7.18)％,(30.19 ± 8.72)％,(30.71±9.90) ％ in posttreatment 1 month,2 months and 3 months,respectively.The efficiency was 95.70％,91.76％ and 88.05％ in 1 month,2 months and 3 months.Conjunctival hyperemia was observed in 13％ eyes at the end of following-up.No drug-related systemic and other ocular adverse reactions were found.Conclusions Domestic latanoprost drops can low IOP effectively without serious adverse reaction in POAG and ocular hypertensive patients.

Objective To evaluate the effect of mining waste water on drinking water quality and tumor mortality of villagers. Methods Analyse quality of drinking water sampled from well which only supply water to a family which the well belongs to and survey the death reasons of whole population in the polluted area and the control area. Results Mining waste water discharged by a mine contaminated the nearby river and impacted the drinking water quality of the nearby village also. Compared with the control, the concentration of lead, arsenic and mercury in drinking water of polluted area was much higher. The 5,year total mortality in polluted area and in the control was 3.09% and 1.55% respectively. Tumor mortality in polluted area was 50%, esophagus cancer mortality was 54.24%. Tumor mortality in the control area was 20% only and most tumors were liver cancer. Conclusion The adverse effect of mining waste water on drinking water quality and human health in polluted area has been seen.

cases of plasma cell mastitis were treated from 1986 to 1999,of whom 37(32.5%) suffered from subareolar abcess and sclerosis,77(67 5%)had subareolar fistula.There were 58 cases(50 8%)of crater nipple.We adopted an arc incision along the edge of areolamammae to remove the necrotic tissues of the ducts,followed by everting suture and mamilliplasty.104 cases(91 2%)achieved healing by first intention.The operations were well done with little injury.Moreover,patients would be satisfied with the appearance.

0. 05). There were no complications during EEVL. During a follow-up of 3 to 30 months in 76 patients with cirrhosis, the mortality was 14. 5% and 2 patients rebled in the near future. Conclusion It is concluded that EEVL is safe, effective, reliable and fast measure in treating esophageal varices bleeding.

ObjectiveTo study the expression and significance of NDRG1(N-myc downstream regulated gene-1), p53 and VEGF in esophageal squamous cell carcinoma (ESCC). MethodsNDRG1, p53 and VEGF protein were detected by immunohistochemistry (IHC, SP method) in 20 cases of normal esophageal squamous epithelium and 78 cases of ESCC.ResultsThe results of IHC shows that in normal esophageal squamous epithelium and esophageal squamous cell carcinoma,the positive rate of NDRG1 was 100.0 ％(20/20) and 55.1％ (43/78) respectively, p53 was 0 (0/20) and 65.4 ％ (51/78) respectively, VEGF was 30.0 ％(6/20)and 67.9 ％(53/78)respectively,all had statistical significance.There was inverse correlationof NDRG1 expression and lymphatic invasion(r =-0.237,P = 0.036).However expression of NDRG1 was no statistical significance with patient' s age,gender,grade,TNM stage,patient' s five year survival.The expression of p53 was inverse correlated with NDRG1,and the expression of VEGF was inverse correlated with NDRG1 (r =-0.331, P = 0.003). ConclusionNDRG1 may be a new tumor suppress gene and play an important role in the development and metastasis of ESCC.

ObjectiveTo evaluate the effect and safety d sorafenib in treatment of patients with metastatic renal cell carcinoma.Methods40 patients with metastatic renal cell carcinoma,were given sorafenib tablets methanesulfonate treatment,the initial dose of 800mg/d,bid,continuous administration of 21d,stopping 7d,The adverse reactions,efficacy and immunohistochemistry results were observed.Results40 cases with metastatic renal cell carcinoma were not found complete remission (CR) and partial remission (PR) ; The 32 cases in stable disease (SD)(80.0％ ) and 8 cases ( 20.0％ ) in PD; The incidence of 28 cases ( 70.0％ ) in digestive system; The expression of positive rate 30.5％ of local in connexin 32,Cx32 was significantly lower than that 1.2％ in metastatic renal cell carcinoma( x2 =8.123,P ＜0.01 ),The expression of Cx32 was negatively correlated with the clinical stage( r =-0.419,P ＜0.05 ) ;The expression positive rate of vascular endothelial growth factor(VEGF) in renal cell carcinoma was significantly higher than 18.5％ in normal renal tissue( x2 =8.723,P ＜ 0.01 ) ; The expression positive rate 72.0％ in the limitations of renal cell carcinoma and metastatic renal cell carcinoma 89.1％ was not statistically significant( x2 =1.978,P ＞ 0.05 ).ConclusionSorafenibin for advanced kidney cancer had better disease control effect,and was new choice for treatment of metastatic renal cell carcinoma.

Objective To explore the correlation between serum hs-CRP and β-cell finction in patients with gestational diabetes mellitus(GDM).Methods The levels of hs-CRP in 60 patients with GDM(GDM group)and 30pregnant women with normal glucose tolerance(NGT group)were detected.Insulin resistance was assessed by the homeostasismodel insulin resistance index(HOMA-IR),Insulin secretion by the homeostasis β-cell funetiOn index(HOMA-β).Results The levels of hs-CRP and HOMA-IR were higher in GDM group than in NGT group.There was significant difference between two groups(P＜0.01);HOMA-β was lower in GDM group than in NGT group,there was significant difference between two groups(P＜0.05).The level of hs-CRP was correlated with age,pre-pregnant bodymass-index(BMI),screening BMI,fasting blood glucose(FBG),fasting insulin(Fins),and HOMA-IR(r=0.222,0.649、0.862、0.923、0.935、0.941,P＜0.05 or P＜0.01),but was inversely related with HOMA-β(r=-0.872,P＜0.01).Multiple stepwise regression analysis indicated that HOMA-IR and HOMA-β was the most important effect factors of hs-CRP.Conclusion The level of hs-CRP increased in women with GDM.which was related to insulin resistance(IR)and insulin secretion,and it maybe participate in the pathogenesis of GDM.