After the practical experiences with embedded subject service for research in nursing science in Library , The First Affiliated Hospital of Chongqing Medical University was described , such as constructing and recommending resources for nursing staff , helping them to search and read related resources , providing training service of how to write and submit papers , the models of embedded subject service for research in nursing science were summarized , such as laying equal stress on construction and recommendation of resources , systematic service in the embedding processes , and tackling problems by groups in coordination .

Objective To observe the efficacy of acupuncture at Hegu (LI4) in treating central facial palsy due to cerebral ischemia, and to explore the dose-effect correlation and the optimal acupuncture protocol.Method Totally 150 patients with central facial palsy due to ischemic stroke were randomized into four groups to receive acupuncture at Hegu with different stimulation parameters plus the conventional integrated Chinese and Western medicine (group A, B, C, D) according to the acupuncture time and direction, and a control group (group E) only to receive the conventional Chinese and Western medicine treatment. The treatment lasted 14 d. The House-Brackmann facial nerve grading system, Toronto Facial Grading System and facial palsy grading scale were adopted as the evaluation indexes.Result After intervention, the scores of the three indexes were all significantly increased in the five groups (P<0.05), and the inter-group comparisons showed significant differences in comparing the improvement rate (P<0.05).Conclusion The study shows that acupuncture at Hegu is effective in treating central facial palsy due to cerebral ischemia, and with the inverse insertion of the needle and manipulation for 5 min at Hegu should be the optimal protocol regarding the improvement of the symptom score and effective rate.

The visual angle of active participation reference model was not scientific and its contents were not sys-tematic in the past EBM information service. A new EBM information service model was thus established by compa-ring the finding process of evidence and the transforming process ofinformation-knowledge-intelligence with the need of evidence-based decision making in clinicians as a logic starting point in order to provide the theory of what to do andhow to scientifically do for clinical librarians and clinicians, to effectively direct clinical librarians to perform EBM information service, and to help clinicians to scientifically make their decision.

OBJECTIVE:To strengthen quality management of drug microbiology laboratory and provide reference for relevant inspection institutions. METHODS:Taking measures from the laboratory document management,personnel training,quality con-trol,quality supervision,process control and biological safety,the elements of laboratory quality management were analyzed,and effectively measures were put forward to improve the management level. RESULTS & CONCLUSIONS:The laboratory should es-tablish strict,standardized,systematic laboratory management rules and regulations,quality documents,operating procedures, work guide,etc. to achieve documented management;develop various forms of annual training and assessment programs to con-struct a professional team;strengthen internal(developing an annual internal quality monitoring plan,conducting quality control ac-tivities,regularly checking the suitability of the medium) and external (capability testing,inter-laboratory comparison) manage-ment control level,and reasonably formulate the contents and frequency by combing with actual situation. Besides,the laboratory should strengthen inspection process control,attach great importance to the biological safety management to reduce the risk of labo-ratory quality to a minimum.

Objective To analyze the dermatology journals indexed by SCI‐E and papers published in recent 5 years ,to pro‐vide advices on periodical selection and contribution .Methods We mainly adopted the bibliometrics methods and word frequency a‐nalysis method to analyze 10 targets in 62 SCI‐E dermatology journals .Results Main publishing nationality were United States , England ,Denmark and Germany ,publishing language was English ,main publishing cycle period was bimonthly journals ,the highest impact factor was 6 .372 .The theme of published content was relatively concentrated ,reviewers′duration was fast and reasonable , 80 .7% submission of the journal was easy .Main type of literature published in recent 5 years is article ,review ,editorial and letter . 58 SCI‐E dermatology journals accept articles from China .Conclusion We suggest that the authors should firstly check the name of dermatology and cross‐disciplinary journals indexed by SCI‐E ,and evaluate the theme of published content ,rate of publishing article contributed from China ,difficulty degree for contribution ,reviewers′duration ,publishing cycle and impact factor when contribution articles .

Objective To investigate the correlation between the distribution of the charactcristics of TCM syndrome of alcoholic liver fibrosis and alcoholic consumption. Methods Our group studied clinical cases in multi-center prospective method. In virtue of Factor Analysis, we explored the characteristics of TCM syndrome of alcoholic liver fibrosis, and the forming characteristics of alcoholic liver fibrosis in inland. Rerults Among the subjects, the characteristics of TCM syndrome of alcoholic liver fibrosis mainly manifested excess syndrome with the common manifestations of deficiency and excess. The visceral position was mainly in liver and spleen, related to gallbladder and stomach. The result suggested that stagnation of Qi stagnation, blood stasis, damp heat, with deficiency of genuine Qi were the interpretation of the causes of alcoholic liver fibrosis. The total alcoholic consumption was one of the important factors which effected on the distribution of thecharacteristics of TCM syndrome of alcoholic liver fibrosis. Total bilirubin had mostly striking effect on the distribution of the characteristics of TCM syndrome. Conclusions The total alcoholic consumption was one of the important factors which effected on the distribution of the characteristics of TCM syndrome of alcoholic liver fibrosis.

Objective To investigate if pretreatment with repeated electroacupuncture (EA) at Baihui acupoint could increase HSP 70 expression after cerebral ischemia-reperfusion injury in rats.Methods Twenty male SD rats weighing 280-320 g were randomly divided into 2 equal groups of ten : control group received no pretreatment and EA group received pretreatment with EA at Baihui acupoint 30 min a day for 5 days. The animals in both groups were anesthetized with isoflurane. Right common carotid artery (CCA) , external and internal carotid artery (EGA, 1C A) were exposed through a longitudinal incision in the neck. EGA was ligated and a 3-0 nylon monofilament (Ethicon) with rounded tip was inserted into ICA and threaded cephalad until resistance was felt. In this way middle-cerebral artery was occluded for 120 min in both groups. The nylon filament was then withdrawn to allow reperfusion. The animals were allowed to recover. At the end of 24 h reperfusion, the animals were anesthetized with intraperitoneal pentobarbital 40 mg?kg-1 and killed. Brain was immediately removed. Two pieces of brain tissue were obtained from right parietal lobe for microscopic examination (HE staining) and determination of expression of heat shock protein (HSP) 70 using immuno-histochemistry technique.Results In EA group the levels of HSP 70 expression were significantly increased (1.94?0.57) compared with those in control group (0.86?0.35) ( P

Objective To establish an HPLC method for the simultaneous determination of hydroxysafflor yellow A, rutin, quercetin, and kaempferol in Carthamus tinctorius L..Methods The Agilent ZORBAX SB-C18 column (4.6 mm × 250 mm, 5 μm) was used with diode array detection. The mobile phase was 0.5% phosphoric acid (A) and methanol (B) to separate the four components by gradient elution at 30℃; the flow rate was 0.8 mL/min; the injection Volume was 10 μL; the detection wavelength was at 360 nm.Results The linear ranges of hydroxysafflor yellow A, rutin, quercetin, and kaempferol were 0.0776–0.7760 μg (r=0.9999), 0.0460–0.4600 μg (r=0.9996), 0.0064–0.0640 μg (r=0.9999) and 0.0128–0.1280 μg (r=0.9997), respectively. The average recoveries of four components were 99.68% with RSD=2.29% (n=6), 99.78% with RSD=1.52% (n=6), 102.03% with RSD=1.02% (n=6), 97.03% with RSD=2.05% (n=6), respectively.Conclusion The method is convenient, accurate, sensitive, stable and repeatable, which can be a method for quality control of Carthamus tinctorius L..

To explore the protective effect of adenovirus mediated IGF-Ⅰgene(Ad-IGF-Ⅰ)transfer on non-obese diabetic(NOD)mice. The results showed that the incidence of diabetes and degree of insulitis were significantly reduced in mice receiving Ad-IGF- Ⅰ . Treatment with Ad-IGF- Ⅰ significantly decreased apoptosis rate,expression of Fas and caspase-3, and increased expression of Bcl-xl. This study indicates the potential of IGF- Ⅰ gene therapy in protecting NOD mice from insulitis and apoptosis.

Objective To establish an HPLC method for the content determination of gallic acid and kaempferol-3-O-rutinoside inNymphaea candida Presl..MethodsThe separation was carried out on a Wondasil C18 column (250 mm×4.6 mm, 5μm). The mobile phase was methanol-0.1% acetic acid solution, with gradient elution;the flow rate was 1.0 mL/min;the detection wavelength was set at 270 nm;the column temperature was 30℃.Results The linear ranges of gallic acid and kaempferol-3-O-rutinoside were 0.065-0.585μg (r=0.999 1), 0.133-1.197μg (r=0.999 5), respectively. The average recoveries of gallic acid and kaempferol-3-O-rutinoside were 102.71%, 102.08%, with RSD of 1.97%, 0.46%, respectively.Conclusion This method was rapid, accurate, and reliable. It can be used for the determination of allic acid and kaempferol-3-O-rutinoside in Nymphaea candidaPresl..