1.Effects of berberine on the proliferation and differentiation of HL-60 cells
Chinese Pharmacological Bulletin 2003;0(11):-
Aim To study effects of berberine on the proliferation a nd differentiation of HL-60 cells. Methods Cell inhibitory effec t was determined by growthcurve method and colony formation.Cell differentiation was analyzed by cytomorphological changes, NBT reduction and cell surface antig en.Cell cycle was analyzed by flow cytometry.Results The growth o f HL-60 cells was significantly inhibited by berberine in a time and dose-depe ndent manner.1,2,4,8 mg?L -1 berberine induced differentiation of HL-60 c ells: Morphological evaluation indicated that the berberine-treated cells exhibited granulation appearance; NBT reduction was significantly increase d; expression of CD11b increased obviously. Flow cytometry analysis showed that berberine arrested HL-60 cells in G 0/G 1 phase, the percent of the cells in S phase decreased.Conclusion Berberine can induce differentiatio n and growth inhibition of HL-60 cells
2.Effect of botulinum toxin type A injection on the expression of substance P, TGF-β1 and α-SMA in rabbit ear model of hypertrophic scar
Lin WANG ; Ningzheng TAI ; Zhihong FAN
Chinese Journal of Medical Aesthetics and Cosmetology 2009;15(6):410-413
Objective To investigate the effect of botulinum toxin type A injection on the expres-sion of substance P, TGF-β1 and α-SMA in rabbit ear model of hypertrophic scar. Methods The hyper-trophic sear model was established in 24 Japanese rabbits'ears. The wounds in ventral surface of ear were divided in Group I (lateral wounds) and Group S (medial wounds), 3 wounds each side per ear, totally 72 wounds each group. The wound-healing time and the growth of scar were observed and recorded. On post-wounding day 28, the wounds were created in another 6 rabbits in the same way and the normal skin were harvested as Group C. Likewise, the scar samples in Groups I and S were harvested. The mRNA expression of substance P, TGF-β1 and α-SMA were detected quantitatively by using real-time PCR and α-SMA was also detected with Western blot. Results No difference between the ratio of healed or infec-tious wounds on post-wounding day 14. The mRNA expression of SP, TGF-β1 and α-SMA in Group I was significantly lower than Group S, but higher than those in Group C (P<0. 01 and <0. 05, respec-tively). The blot density of α-SMA and the ratio of blot density of a-SMA to β-actin in Group I were be-tween that of the other two groups. Conclusion Without delaying the wound-healing, botulinum toxin type A injection significantly decreases the expression of SP, TGF-β1 and α-SMA in rabbit ear model of hypertrophic scar.
3.The mini-laparoscopic management of indirect inguinal hernia in children
Zhihong LAI ; Yanjun ZHENG ; Xiaobin LIN
Chinese Journal of Primary Medicine and Pharmacy 2010;17(8):1041-1042
Objective To study the method, advantage and shortcoming of mini-laparoscopic treatment for indirect inguinal hemia in children. Methods 136 cases with indirect inguinal hernia(right:75cases,left:44 cases,bilateral:17 cases) had been treated by high ligation with mini-laparoscopy. Results The mean operation time was 10 mmin for unilateral hernia and 23 mmin for bilateral hernia. Among 119 cases with unilateral hernia,38 cases had been found with contralateral latent hernia. The mean of postoperative inhospital stay were about 1 ~ 2 day(s). All patients had been followed-up for 3 ~ 12 months,there were only 2 cases recurrence found but no complications was detected.Conclusion Mini-laparoscopic high ligation for indirect inguinal hernia in children is a single, safe, satisfactory, effective,and minimally invasive surgical method.
4.Neurological recovery effect of blood activating and phlegm removing decoction in treatment of the patients with hypertensive cerebral hemorrhage
Shanshan JIANG ; Zhihong FU ; Lin CHEN
Chinese Journal of Primary Medicine and Pharmacy 2016;23(8):1252-1255
Objective To investigate neurological recovery effect and mechanisms of blood activating and phlegm removing decoction in the treatment of patients with hypertensive cerebral hemorrhage.Methods 68 patients with hypertensive cerebral hemorrhage in our hospital were divided into two groups.34 patients of the control group were given conventional western medicine,and 34 patients of the observation group were taken conventional western medicine combined with blood activating and phlegm removing decoction.The clinical outcomes, hematoma volume change and neurological recovery were compared,and analyzed its mechanism of action.Results The effective rate of the observation group(94.12%) was significantly higher than 73.53%of the control group (χ2 =5.31,P<0.05). After 7d,14d,21d treatment,NIHSS scores of the observation group [(12.87 ±1.43) points,(8.55 ±1.11) points and (5.67 ±0.76) points] were significantly lower than those of the control group [(20.43 ±2.57) points, (17.66 ±2.08)points,(14.54 ±1.44)points],and the differences were statistically significant (t=14.99,22.53, 31.76,all P<0.05).After treatment,hematoma volume of the observation group[(6.44 ±0.54)mL]was significant-ly lower than before treatment[(16.30 ±1.90)mL]and after treatment in the control group [(12.11 ±1.44)mL] (t=29.11,21.50,all P<0.05).After treatment,ALD score of the observation group [(66.34 ±5.22)points] was significantly lower than before treatment[(27.53 ±2.20)points]and after treatment in the control group[(47.55 ± 3.33)points],and the differences were statistically significant(t=39.95,17.70,all P<0.05).Conclusion The effect of blood activating and phlegm removing decoction in the treatment of patients with hypertensive cerebral hemor-rhage is significant,can significantly improve neurologic impairment and daily living skills,has no obvious conflict of interest and high application value,worthy of promotion and application.
5.Clinical effect analysis of blood coagulation therapy in patients with gastric ulcer bleeding treated by omeprazole and Agkistrodon acutus venom and changes of serum vascular endothelial growth factor and epidermal growth factor levels in serum
Aimin ZHANG ; Zhihong LIN ; Jiangfeng DAI
Chinese Journal of Biochemical Pharmaceutics 2017;37(4):336-338
Objective To investigate the clinical effect of coagulation therapy in patients with gastric ulcer bleeding.Methods From January 2013 to January 2016 in Xiaoshan First People's hospital 150 cases of gastric ulcer patients with bleeding,using SPSS16.0 software to generate randomly divided into hemocoagulase group(basic treatment + hemocoagulase injection therapy)and control group(basic treatment)of the 75 cases,compared two groups of hemostatic time,hemostatic effect.Results After 72h treatment,the average hemostasis time of hemocoagulase group were significantly lower than the control group(P<0.05),the blood coagulation enzyme in patients with serum VEGF,EGF levels higher than the control group(P<0.05),gastrin and somatostatin levels lower than the control group(P<0.05),hemocoagulase group cure rate is 64%,effective in 22.67%,effective 9.33%,invalid 4%,the control group were 46.67%,25.33%,18.67%,9.33%,two groups compare difference was statistically significant(P<0.05).Conclusion Treatment of gastric ulcer bleeding patients with coagulation enzyme can shorten the hemostatic time,improve the clinical effect of hemostasis.
6.Application of early post-operative enteral nutrition in eldly patients with gastric neoplasm
Weijun ZHANG ; Zhihong LIN ; Zhenyu WANG ;
Parenteral & Enteral Nutrition 1997;0(04):-
Objectives: To explore the infulence of early enteral nutrition on nutritional and immunal status and complications for elderly patients with gastric neoplasm. Methods: 84 cases of gastric neoplasm were randomly divided into TPN group( n =42),an EN group ( n =42).Equal calorie and nitrogen were given from the frist day after operation. Nutritional and immune markers were examined before and after nutrition support. The complication and gastrointestinal function were carefully observed . Results: Nutritional markers and immune markers were significantly increased after nutritional support both in TPN and EN group ( P 0.05). EN can promote resumption of gastrointestinal function,decrease complications and reduce the medical cost. Conclusions: Early post operative enteral nutrition for elderly patients with gastric neoplasm is a safe,effective,economic nutritional treatment.
7.Changes in NO content and NOS activity in serum and testis in diabetic rat
Zhihong DENG ; Xiaoyan LIN ; Wei WANG
Medical Journal of Chinese People's Liberation Army 1982;0(03):-
Objective To observe the changes in NO content and NOS activity of serum and the testis in Wistar rats with type I diabetes mellitus. Methods 63 rats were randomly divided into control group(C) and diabetes group(D). Diabetes was induced by intraperitoneal injection of Alloxan. Rats with diabetes were observed for 1 week(D 1), 2 weeks(D 2), 3 weeks(D 3), 4 weeks(D 4), 5 weeks(D 5), 6 weeks(D 6), 7 weeks(D 7) and 8 weeks(D 8), respectively, and they were sacrificed at the end of each observation period. Serum was obtained and testis homogenates were prepared, and the levels of NO content and NOS activity of them were determined respectively. Results Compared diabetes group with control group, NO contents and NOS activity of serum from diabetic rats were higher, then NOS activity began to decline to approach the control level. In the testis homogenates, NO content dropped slightly after injection of Alloxan, while NOS activity showed no significant change. Thereafter, both NO content and NOS activity of testis homogenates were increased. Conclusion There are changes in NO content and NOS activity of serum and testis of rats after diabetes are induced with Alloxan.
8.Protective effects of mycelium of cultured Cordyceps sinensis on testis in diabetic rats
Zhihong DENG ; Xiaoyan LIN ; Weimin LI
Medical Journal of Chinese People's Liberation Army 1981;0(04):-
Objective To observe the protective effects of mycelium of cultured Cordyceps sinensis on testis in diabetic Wistar rats. Methods 21 rats were divided into control group(C), diabetes group(D) and treatment group (T) at random. Diabetic model was reproduced by intraperitoneal injection of alloxan. After having been treated with Jinshuibao capsule for 8 weeks, serum levels of glucose, insulin, testosterone and levels of high energy phosphate compound, MDA, SOD, GSH-Px, and ATPases in the testis were determined respectively. Results Compared with control group, testicular mitochondria showed lipid peroxidase injury, with disturbed energy metabolism, and the activity of ATPases was lowered. All the changes in serum and testes were improved when Jinshuibao capsule was given. Conclusion Mycelium of cultured Cordyceps sinensis might have protective effects on the testis in diabetic Wistar rats.
9.Phosphorothioate-modified antisense TGF-β1 oligodeoxynucleotide inhibits neointimal hyperplasia after vascular balloon injury in rats
Zhihong LIN ; Liangdi XIE ; Kegui WU ; Gengshan LI ; Peihong LIN
Chinese Journal of Pathophysiology 2014;(8):1433-1438
[ABSTRACT]AIM:ToevaluatetheeffectsofantisenseTGF-β1oligodeoxynucleotide(ASTGF-β1)ontheex-pression of TGF-β1 , deposition of extracellular matrix ( ECM) and the neointima formation in the arteries after balloon inju-ry.METHODS:The unmodified and phosphorothioate-modified AS TGF-β1 which containing 15 bases and surrounding the initiation codon region (ATG) of rat TGF-β1 complementary DNA (cDNA) were designed.At the same time, sense TGF-β1 oligodeoxynucleotide ( S TGF-β1 ) with the base sequence complement to AS TGF-β1 was synthesized as a control . The oligodeoxynucleotides were introduced into in vivo and in vitro experiments , respectively .RESULTS:The AS TGF-β1 significantly inhibited the protein expression of TGF-β1 in a concentration-dependent manner , and S TGF-β1 did not have the same effect.Furthermore, no effect of the AS TGF-β1 on the mRNA expression of TGF-β1 in injured VSMCs was ob-served.Moreover, for the injured VSMCs, AS TGF-β1 significantly and concentration-dependently inhibited the basal DNA synthesis.Both AS TGF-β1 and S TGF-β1 did not exhibit dose-dependent effects on DNA synthesis in uninjured VSMCs . Fibronectin ( FN) mRNA expression in injured VSMCs was significantly decreased by AS TGF-β1 in a concentration (0.01~1 μmol/L)-dependent manner .AS TGF-β1 significantly increased the mRNA expression of contractile marker SM 22α, and decreased the mRNA expression of synthetic markers osteopontin and matrix Gla , especially at the concentration of 0.01μmol/L and 0.1 μmol/L.After treatment with AS TGF-β1 (90 μg· kg-1 · d-1 ) for 28 d, the neointima formation was significantly inhibited , and the area ratio of intima/media was markedly decreased by 68% compared with untreated group , but S TGF-β1 had no effect on neointimal formation .CONCLUSION:The AS TGF-β1 specifically inhibits the pro-tein expression of TGF-β1 in the VSMCs derived from injured arteries .Moreover , it significantly inhibits DNA synthesis and cell proliferation, and decreases the expression of FN .Therefore, AS TGF-β1 dramatically attenuates neointima formation after balloon njury .The effects of AS TGF-β1 on the injured VSMCs may be associated with its reverse effects on the altera-tion of VSMC phenotype after balloon injury .