1.Influence of Safflower(Carthamus tinctorius)on Ca~(2+) Transmembrane Influx in Rat Aorta
Chinese Traditional and Herbal Drugs 1994;0(10):-
Influence of Carthamus tinctorius L. has been studied on Ca2+ transmembrane influx in rat aorta when its extract was used at 5, 50, 500?g/ml. It was shown that the resting 45Ca influx in rat aorta ring was not altered by C. tinctorius but the 45Ca influx evoked by norepinephrine (1.2 x 10-6 mol/L ) and KCl solution (100mmol/L) was inhibited in a concentration-dependent manner. The results demonstrated that the receptor-operated Ca2+ channel (ROC) and potential-dependent Ca2+ channel (PDC) in cell membrane of smooth muscle could be blocked by C.tinetorus as well as by verapamil.
2.Progress in the pathogenesis of obesity-induced hypertension
Journal of Chinese Physician 2017;19(5):798-800,封3
Obesity is one of the major risk factors of primary hypertension,with the dramatic increase in obesity.Obesity-induced hypertension also gradually increases.Due to promoting the occurrence and development of high blood pressure,obesity has caused wide public concern.In recent years,the pathogenesis of obesity-induced hypertension has become the study hotspot.However,fat and blood pressure relationship is complicated.This review article emphatically reviewed the activation of the sympathetic nervous system,the renin-angiotensin-aldosterone system (RAAS) activation,insulin and insulin resistance,leptin and leptin resistance,the gastrointestinal tract anomalies in the pathogenesis of obesity hypertension of the latest research progress.
3.Effect of polyene phosphatidyl on synaptic plasticity in region of hippocampus CA3 of young rats
Sixun AN ; Xiaoli CHEN ; Xin HE ; Zhiheng DONG
Journal of Jilin University(Medicine Edition) 2006;0(03):-
Objective To observe the structure changes of the synapse of the neurons in hippocampus CA3 of young rats and study the basis for the mechanism of polyene phosphatidyl in providing learning and memory and the effect on synaptic plasticity.Methods A total of 20 Wistar rats with 5 months were randomly divided into polyene phosphatidyl group and normal control group.Each group had 10 rats.After 4 weeks feeding,Water maze training was peformed in all the rats for 1 weeks.The immue expressions of synapsin(SYN) of the rats in polyene phosphatidyl group and control groups were observed with immunohistochemical method and analyzed by MOTAC imagine analyzing system.The change of synapse of hippocamal CA3 was observed with electron microscope.And the other 24 to 26 months rats were selected as aged group,and fed in the same condition.Moreover,the ultrastructures of hippocamal CA3 of aged rats were observed by transmission electron microscopy(TEM).Results The SYN in polyene phosphatidyl group(0.430 0?0.022 4) was higher than that in control group(0.3567?0.0209) (P
4.Relationship between the Th1/Th2 Cytokines Imbalance and Autoimmune Thyroid Diseases
Yamei TANG ; Zhiheng CHEN ; Aiguo TANG
Journal of Chinese Physician 2001;0(07):-
Objective To analyze the relationship between serum Th1/Th2 cytokines levels and autoantibodies against thyroid, and explore the role of Th1/Th2 cellular immunity imbalance in the pathogenesis of autoimmune thyroid diseases(AITD). Methods 21 patients with Graves'desease(GD), 18 cases with Hashimoto's thyroiditis(HT), 17 cases with non-toxic nodular goiter(NTNG) and 20 healthy subjects were enrolled in this study. The serum concentrations of their Th1 cytokines (IFN-?,IL-2) and Th2 cytokines (IL-4,IL-10) were assayed by ELISA. The serum levels of their thyrotropin receptor antibodies(TRAb), thyroglobulin antibodies (TGAb) and thyroid peroxidase antibodies(TPOAb) were measured by routine methods. The relationship between the serum Th1, Th2 cytokines levels and serum TRAb, TGAb, TPOAb levels were analyzed. Results The serum levels of IL-4 and IL-10 in patients with GD were significantly higher than those in patients with HT,NTNG and healthy subjects(P
5.Relationship Between the Serum Indices of Liver Fibrosis and TCM Syndromes Types in Liver Cirrhosis due to Chronic Hepatitis
Zhiheng PAN ; Yanbi CHEN ; Zide DENG
Journal of Traditional Chinese Medicine 1992;0(08):-
In order to probe into the relationship between the TCM syndrome types and the five indices of fibrotic markers of liver in serum, the HA, hPC I , IV - C in 160 of liver cirrhosis patients induced by chronic hepatitis were examined. Results showed that the 5 indices in all TCM syndrome types were all higher than the control group, each with its specificity. The increased amplitude in the group of liver - stagnant, spleen - deficiency was the least one. It was also found that the 3 indices of liver fibrosis in the group of internal blockade of damp - heat were markedly increased; it was mainly the increase of HA, hPC I in the group of yin-deficiency of both liver - kidney and obstruction of collaterals by static blood; but it was mainly the increase of HA, W - C in the group of Yang - deficiency of spleen and kidney. This demonstrated that the above indices were of significance in TCM syndrome typing for chronic hepatic victims. Lingering of damp -heat may be the trigger factor for liver fibrosis in which blood stasis was present during the whole course of liver fibrosis.
6.Health risk warning system and its follow up results
Guilian WANG ; Zhiheng CHEN ; Nana TAN
Chinese Journal of Health Management 2014;8(2):95-98
Objective To explore the reporting process of our health risk warning system and its long-term effectiveness.Methods A total of 1 168 individuals who were identified as higher risk populations from July 2011 to June 2012 in our Health Mangement Center were served as the control group,and another 973 adults who were identified as higher risk individuals from July 2012 to May 2013 were assigned to the study group.Diagnosis and treatment were based on our health risk reporting process and follow-up system.Paired t test and paired contingency table x2 test were used for data analysis.Results The rate of follow-up (97% vs 100%,x2=30.503,P<0.05),consultation (83.48% vs 93.63%,x2=52.142,P< 0.05),mean treatment time ((3.0±0.5) vs (1.5±0.5) d,t=69.12,P<0.05) and patient satisfaction (87.84% vs 96.20%,x2=48.361,P<0.05) were significantly different between the two groups,although no statistically significant difference of incidence of adverse events was found (0.26% vs 0.10%,x2 =0.102,P>0.05).Conclusion Our health risk warning system may reduce the time for physical examination and improve disease diagnosis level and patients' satisfaction.Moreover,our health manatement system and health service quality should further be improved in practice.
7.The expression of death receptor 6 in the cerebral cortex in neonatal rats after hypoxia ischemia
Lili SONG ; Zhiheng HUANG ; Chao CHEN
Journal of Clinical Pediatrics 2013;(12):1159-1162
Objectives To observe the expression of death receptor 6 (DR6) in neonatal rats with hypoxia-ischemia brain damage (HIBD). Methods HIBD was induced in day 7 rats. The expression of DR6 at 24 h, 72 h and 7 d after HIBD and the expression of Caspase-3 at 24 h were evaluated by immunostaining. The injury of neural cells was evaluated by cresyl violet at 7 d after HIBD. The cognitive function was evaluated by T-maze test at 60 d after HIBD. Results DR6 positive cells were the most abundant in the ipsilateral cortex at 24 h after HIBD, and decreased gradually at 72 h and 7 d after HIBD. There was signiifcant difference of the expression of DR6 among different time points in HIBD group (P<0.01). Compared with control group, DR6 positive cells were more abundant in the ipsilateral cortex at 24 h and 72 h after HIBD (P<0.01) and caspase-3 positive cells were more abundant in the ipsilateral cortex at 24 h after HIBD (P<0.05). The number of cortical neurons were decreased at 7 d after HIBD as compared with control group (P<0.05). The T-maze test showed there was decline of the cognition in HIBD group com-pared with control group (P<0.05). Conclusions The DR6 signaling pathway plays an important role in cerebral cortex injury which may lead to the subsequent neurofunctional deifcits in neonatal HIBD rats.
8.Longterm influence of hypoxic-ischemic brain damage in 3-day-old rats on their brain MR imaging and memory and learning ability
Jiangqin LIU ; Zhiheng HUANG ; Qingguo WANG ; Chao CHEN
Chinese Journal of Perinatal Medicine 2009;12(3):205-208
Objective To investigate the activation of apoptotic genes of the brain with hypoxia- ischemia (HI) in newborn SD rats, and MRI changes and memory and learning ability in adulthood. Methods HI was induced by right carotid artery ligation followed by 2.5 h of hypoxia (6% O2) on 3-day-old SD rats (n=36). Control pups were sham-operated (n = 27). Right brain hemisphere was collected at 12 h and 7 d after HI and subjected to an apoptosis Oligo GEArrayR. MRI and Morris water maze test were performed on both groups at 42 and 44 days old, respectively. Results Comparing to 12 h after HI, up-regulated apoptotic genes included TNF, Caspase and pro-apoptotit genes of Bcl2 families, whereas the anti-apoptotic genes of Bcl2 family were down-regulated at 7 d after HI. The MRI assessment of the rats in HI group demonstrated that the area of the right cerebra l cortex was significantly smaller than the left side and control [periventricular layer: (23.5±3.6) mm2 vs (33.0±4.3) mm2, (34.5±3.9) mm2; hippocampus layer: (18.9±4.4) mm2 vs (29.1±5.0) mm2,(30.8±4.5) mm2, both P<0.01]. During the navigation trial, the HI rats demonstrated longer escape latency (4th day: (52.7±35.9) vs (17.8±8. 9) s, P<0.01). HI rats passed the platform less times than the control ones (T= 292.5, P<0.05) in space probe trial. Conclusions The activation of apoptotic genes induced by HI brain injury remains until 7 days later, involving intrinsic and extrinsic apoptotic pathway. The apoptosis of neural cells may lead to poor development of the cortex and impair the memory and learning ability in the adult rats after neonatal hypoxia- ischemia injury.
9.Application of hypocaloric parenteral nutrition in postoperative elderly patients with gastric cancer
Yuping PENG ; Honggang JIANG ; Zhiheng CHEN ; Bohao LU ; Min WANG
Chinese Journal of Geriatrics 2012;31(3):225-228
Objective To investigate the application of hypocaloric parenteral nutrition in postoperarive elderly patients with gastric cancer. Methods 59 elderly patients aged 60- 79 years with gastric cancer after operation were randomly assigned to receive 84 kJ · kg -1.d -1 hypocaloric parenteral nutrition (29 cases) and 126 kJ · kg-1 · d -1 standard-calorie parenteral nutrition (30cases),totally 6 d from postoperative 2 d. Complications, serum proteins, immune function,inflammation,blood glucose, liver function and recovery time of gastrointestinal function were observed during nutritional support. Results The postoperative complication rate were 37.9%(11/29) in hypocalorie group and 43.3 % (13/30) in standard calorie group ( P>0.05),mainly pulmonary infection.There were no ditferences in levels of serum albumin,total protein and prealbumin between two groups at postoperative 3 d and 6 d (P>0.05).Peripheral blood lymphocytes and T lymphocyte subsets CD3+,CD4+,CD4+/CD8- ratio were decreased after sugery,but CD8 1 values increased in two groups (P>0.05).C reactive protein was increased significantly at postoperative 3 d compared with preoperation,and decreased at 6d than 3 d (P>0.05).Blood glucose was decreased gradually at 3 and 6 d (P>0.05).Postoperative liver function paraneters were elevated,no difference was found at 3 d (P>0.05),and continued to rise at 6 d in standard calorie group than in hypocalorie group (P<0.05). There was no difference in times of aerofluxus and cacation after operation between hypocalorie and standard calorie groups (P>0.05). Conclusions Hypocaloric parenteral nutrition is a safe and effective method for nutrition support in postoperative elderly patients with gastric cancer.
10.Detecting 43Codon of High Mutation Site in rpsL of STR Resistant MTB by Molecular Beacons Technology:An Experimental Study
Qinghai CHEN ; Zhiheng BIAN ; Hong KUANG ; Weiling FU
Chinese Journal of Nosocomiology 2006;0(03):-
OBJECTIVE To design molecular beacon probe of 43 Codon of high mutation site in rpsL of STR resistant MTB and its amplification system, meanwhile, to find the way of detecting the fluorescent light and making a qualitative judgment by use of fluorescence microscope and image analysis software. METHODS The software, Beacon designer, was used to design the 43 Codon molecular beacon probe and the amplification system, and the fluorescence microscope and image analysis software were used to detect the fluorescent light and make a qualitative judgment. RESULTS The strap of amplification product was shown clearly. The difference between PCR products from standard strain and STR resistant one was obvious in detecting the fluorescent light by use of fluorescence microscope. The rate of resistant STR detected was about 80% (P