AIM: To apply UASLG for Rana temporatia chensinensis David's identification. METHODS: Rana temporatia chensinensis David was made ultrasonic extraction. These solvents in turn were petroleum ether、chlorofo mn、ethyl acetate、n-butanol and 95%WTBZ# ethanol,then their absorbance in UV and one-order derivative spectrums were determined. RESULTS: Rana temporatia chensinensis David's UASLG was reproducible. CONCLUSION: UASLG's action spectrums for Rana temporatia chensinensis David can be identified for quality control.