OBJECTIVE To explore the mechanism of total flavonoids from stem and leaf of Scutellaria baicalensis in treating ulcerative colitis based on network pharmacology and animal experiments. METHODS The total flavonoids in stem and leaves of Scutellaria baicalensis were reviewed in literature, the corres ponding targets of total flavonoids in stem and leaves of Scutellaria baicalensis were retrieved by TCM systematic pharmacology database and analysis platform(TCMSP), and the related targets of ulcerative colitis were collected by GeneCards database. The corres ponding targets of total flavonoids from the stem and leaves of Scutellaria baicalensis and those related to ulcerative colitis were in put into Venny 2.1 mapping software to obtain the intersection targets, which were uploaded to STRING data platform for the construction of protein-protein interaction network. The gene ontology(GO) functions and the Kyoto encyclopedia of genes and genomes(KEGG) pathway enrichment were analyzed using the DAVID data platform jointly. The network pharmacological results were verified in a mouse model of ulcerative colitis induced by 3% DSS. RESULTS The results of network pharmacology showed that there were 69 related targets, 1383 related targets in ulcerative colitis and 24 overlapping targets in 7 components of total flavonoids from stem and leaves of Scutellaria baicalae. GO and KEGG pathway enrichment analysis were conducted to obtain the potential pathways of total flavonoids from stem and leaves of Scutellaria baicalensis in the treatment of ulcerative effects, including aryl hydrocarbon receptor(AhR), Th17 cell differentiation, T cell receptor, etc. The results of 3% DSS induced ulcerative colitis mouse model showed that compared with the model group, total flavonoids of scutellaria scutellaria stem and leaf significantly decreased the levels of MPO, TNF-α, IL-1β and IL-17 and increased levels of IL-10 and IL-22 with statistical difference, and restored the colon length of mice. Activated the AhR pathway and its CYP1A1 and CYP1B1 targets to promote the expression of anti-inflammatory factor IL-22, and repair the intestinal barrier by activating ZO-1 proteins. CONCLUSION Total flavonoids from stem and leaf of Scutellaria scutellaria can synergistically treat ulcerative colitis based on the characteristics of multi-component, multi-target and multi-pathway.