The activity of SOD in the plasma of patients with NPC and the effect of the culture supernatant of NPC cell line (CNE-2s) on SOD activity were examined by method of Oyanagui. The results showed that the activity of total-SOD, Mn-SOD, CuZn SOD in the plasma of patients with NPC decreased significantly than those of the normal (P

This experiment was made on 4-6 week-old nude mice(NM). The CNE-2 cells were inoculated subcutaneously into each side of the neckback for the original generation. Implanted fresh transplanted NPC tissue to NM's neck-back with cannula for next passage. We passed up to 8 generations and the transplantability was 100%. We observed dynamicrly the changes of NM's immune status after they have bearing the NPC as following: (1) The net weight was significantly reduced. (2) Splenic index(S. I.) and tumor weight(T. W.) increased. S. I. was highly related to T. W. (r=0.8395). (3) Splenic NK activity slightly increased in 5 days, began to decrease in 10 days and significantly decreased in 15 or more days. NK activity was negative related to T. W. and S. I. markedly (r=-0.7132, r=-0.8237). (4) Splenic NK cells had excellent responsiveness to human IFN in vitro in 5 days and still had some in 10 days, but it had not any after 15 days. (5) Natural proliferative ratio of tumor bearing NM's splenic cells was higher than that of control group, but the responsiveness to ConA was much lower. (6) Ai Ke Xing suppressed the growth of transplanted NPC by means of decreasing sera MDA enhancing host's splenic NK activity and increasing mononuclear infiltration of the transplanted NPC tissue. So we concluded that in NM whose T cells were deficient splenic NK cells were important immunocompetent cells for resisting the growth of transplanted NPC.

Changes of plasma lipid peroxide, production of IL-2 and activity of IL-2receptor of peripheral blood lymphocytes in patients with nasopharyngeal carcinoma(NPC) were reported. The results showed MDA levels in plasma from 35 cases of NPCwere much higher than those of healthy controls (P

Nasopharyngeal carcinoma (NPC) in BALB/c nude mice (NM) was re-produced by hypodermic implanting human CNE-2 cell to the right-side of neck-backposi-tion. The changes of plasma malondialdehyde (MDA) and cAMP/cGMP ratio in NM bea-ring human NPC were examined. Meanwhile, we observed that tumor's size, plasma MDAand cAMP/cGMP ratio in NM bearing human NPC were affected by polysacchoridopetide(PSP). Results indicated that in comparison with the control group, NM bearing humanNPC showed a marked increase in plasma MDA(18.25?1.37 nmol/ml),cAMP/cGMP ratio(4.55?0.35), a marked decrease in plasma, cAMP (71.83?8.51 pM/ml) and cGMP(15.77?0.65 pM/ml), both high or low-concentration PSP inhibited NPC growth.Rate of inhibition was between 59.58 to 95.53 percent. PSP decreased plasma MDA,elevated cAMP, cGMP's levels and recovered cAMP/cGMP ratio. Our results suggestedthat the elevated level of MDA in NM bearing human NPC was closely associated withthe presence of NPC. PSP had antitumor effect, probably by means of decreasing freeradicals and recovering cAMP/cGMP ratio.

This paper reports on the effect of the culture supernatant of NPC cell line (CNE-2S) (400?l/ml) on cAMP、IL-2、and IL-2 receptor in normal lymphocyts. The cAMP levels of lymphocytes from 11 cases was 13.77?4.92pm/1?10~6. With lymphocytes suspensives added to the CNE-2S, the cAMP levels was increased to 26.1? 15.14Pm/1?10~6(P0.05)These results show that the immune function abnormality of the patients with NPC relates to the fact of that the NPC immunologic inhibitory factor declines the ability of lymphocytes to produce IL-2.

Ehrlich's ascites cancer (EAC) in BALB/C mice by ip transplantable EAC cell is reproduced by us. The change of serum malondialdehyde(MDA), plasma cellular cAMP and adenylate cyclase (AC) are examined in the early, middle and late phase of mice bearing EAC respectively in comparison with that of control group. Results indicate that serum MDA in mice bearing EAC is continuously increased with the progress of the disease in the remarked ways in comparison with the control group. Whereas the plasma cAMP in the mice bearing EAC is markedly decreased in that order. Relation between the change of serum MDA and the cellular cAMP, AC or plasma cAMP shows a significant negative correlation efficiency (P

The effects of polysaccharidical peptides (PSP) on the functions of tumour infiltrating lymphocytes (TILs) in vitro was explored. The results showed that PSP, at the concentration of 37-1200?g/ml, promoted the proliferation of TILs in dose-related manner. Combination of PSP with interleukin-2 (IL-2) enhanced the cytotoxicity of TILs and reduced the dose of IL-2 used for activating TILs. The results suggested that PSP could augment the proliferation and cytotoxicity of TILs in vitro.

OBJECTIVETo obtain the peptide that specifically binds to human osteosarcoma MG-63 cells from Ph. D. 7TM phage display peptide library.

METHODSHuman osteosarcoma MG-63 cells were used as the target cells with human embryonic kidney 293T cells as the control for screening the peptide from Ph. D. 7TM phage display peptide library. The enriched specially binding peptides were verified by cell enzyme-linked immunosorbent assay (ELISA). The location of the peptide in MG-63 cells was investigated using cell fluorescence staining, and targeting of the peptide was tested by organ immunohistochemistry with Osteosarcoma model.

RESULTSThe specifically binding peptides were enriched after 4 rounds of screening. The sequence SLTNLSK was confirmed as the most frequent peptide by DNA sequencing and showed strong specificity verified by cell ELISA, fluorescent staining and organ immunohistochemistry.

CONCLUSIONA peptide that specifically binds to MG-63 cells has been screened from Ph. D. 7TM phage display peptide library to serve as a potential candidate for osteosarcoma-targeting therapy.

Amino Acid Sequence ; Animals ; Bone Neoplasms ; drug therapy ; Cell Line, Tumor ; Drug Screening Assays, Antitumor ; Humans ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Osteosarcoma ; drug therapy ; Peptide Library ; Peptides ; analysis ; Protein Binding