1.The effect of basic periodontal therapy on blood glucose control in diabetes patient with periodontitis
Yu ZHANG ; Xue LI ; Zhihai TENG
Journal of Practical Stomatology 2000;0(05):-
Objective:To investigate the effect of basic periodontal therapy on levels of blood glucose,blood lipid,and tumor necrosis factor-alpha(TNF-?) among patients with periodontal diseases in insulin dependent diabetes patients.Methods:Forty insulin dependent diabetes patients with periodontitis were selected and divided into basic periodontal therapy group and control group.The periodontal clinic index,blood glucose,blood lipid,glycosylated hemoglobin(HbA1c),and serum TNF-? concentrations were assessed before treatment and 4 weeks,3 and 6months after basic periodontal therapy.Results:The HbA1c,TNF-?,total cholesterol(TC),tyiglycerides(TG) in basic periodontal therapy group were significant decreased after periodontal therapy,and meanwhile had significant difference compared with that in control group.Conclusion:The basic periodontal therapy can help to control the blood glucose level in insulin dependent diabetes mellitus with periodonitis.
2.Study on Quality Standard of Patch for Hyperosteogeny
Hongyan SHAO ; Heming ZHANG ; Zhihai LIU
China Pharmacy 2007;0(27):-
OBJECTIVE:To establish the quality standard of Patch for hyperosteogeny.METHODS:Mastic and Myrrh in Patch for hyperosteogeny were qualitatively identified by TLC,and the content of paeoniflorin in preparation were determined by HPLC.RESULTS:The identification of TLC has a good resolution and specificity.The linear range of paeoniflorin were 0.204~1.428 ?g(r=0.999 2) with an average recovery of 99.41%(RSD=0.64%,n=6).CONCLUSION:Established standard can be used for the quality control of Patch for hyperosteogeny.
3.The study of enriching peripheral circulating carcinoma cells in colorectal cancer patients by magnetic activated cell sorting
Zhihai PENG ; Fang ZHANG ; Yun LING
Chinese Journal of General Surgery 1993;0(01):-
ObjectiveTo enrich and quantitatively analyze peripheral circulative carcinoma cells by magnetic activated cell sorting (MACS) in colorectal cancer patients.MethodsBlood samples, preoperatively collected from 21 patients with colorectal cancer and 9 healthy volunteers, were labeled by anti-cytokeratin (CK) with magnetic microbeads, and passed through magnetic columns; CK +carcinoma cells were separated from the samples, and quantified by flow cytometry.ResultsCK +CD45 -cells could not be detected from the samples without MACS; in the sample undergoing MACS, the concentrations of CK +CD45 -cells were significantly higher in patient group than in control group(P
4.Attaching Great Importance to the Spirit of Innovation and Improving Application Capacity in Education of Rehabilitation Therapeutics
Xiaojie LI ; Zhihai LV ; Lihua ZHANG
Chinese Journal of Rehabilitation Theory and Practice 2008;14(4):394-395
It is the new aims and requests in the education of rehabilitation therapeutics,paying more attention to be attached importance of spirit innovation and improving application capacity.The authors discussed the new requires and methods applied in Jiamusi University including training the teachers,course offered,teaching methods and education substances and practical teaching,according to analyze the current situation of education in rehabilitation therapeutics in China.
5.Correlation between histone deacetylase 9 gene single nucleotide polymorphisms and ischemic stroke
Ming GAO ; Zhihai WANG ; Lixia XUE ; Shuangyan ZHANG
Chinese Journal of Cerebrovascular Diseases 2015;(5):235-239
Objective To investigate the correlation between histone deacetylase 9 gene ( HDAC9 ) single nucleotide polymorphisms and ischemic stroke ( IS) subtypes. Methods A total of 202 patients with IS were enrolled prospectively. According to the Trial of Org 10172 in Acute Stroke Treatment ( TOAST) classification,the patients with IS were divided into large artery atherosclerotic stroke group (LAA,n=149) and the small-artery occlusive stroke group (SAO,n=53). A total of 201 age- and sex-matched healthy subjects over the same period were enrolled as the control group. Polymerase chain reaction-ligase detection reaction ( PCR-LDR ) was used to perform genotyping for HDAC9 gene rs11984041 and rs2107595 loci in all subjects. The correlation between genotype and IS was analyzed with multivariate logistic regression analysis. Results ( 1 ) In all the subjects detected, the genotype detected from rs11984041 loci was type CC,and 3 genotypes including CC,CT,and TT were detected from rs2107595 loci. (2) There were significant differences in dominance model (CT+TT,CC) genotypes and allele frequencies among the LAA, SAO, and control group dominance model ( CT +TT, CC ) and (χ2 =8. 635,P=0. 013, χ2 =10. 309,P=0. 006);and there were no significant differences in dominance model (CT+TT,CC) and allele frequencies among the 3 groups (after adjustment all P>0. 017). Compared with the control group,there were significant differences in the CC,CT,and TT genotypes and allele frequencies in the LAA group (χ2 =7. 446;P=0. 006). In the dominance models (CT+TT),there was significant difference in genotype frequencies between the LAA group ( 65. 1%,97/149 ) and the control group (52. 2%,105/201) (χ2 =5. 800;P=0. 016). (3) Multivariate logistic regression analysis showed that in addition to gender,hypertension,smoking,and high level of low-density lipoprotein,the dominance model CT+TT genotype was associated with the LAA type IS ( CT+TT genotype:OR,1. 909,95% CI 1. 055-3. 454,P =0. 033). Conclusion In addition to the risk factors for cerebrovascular disease such as gender,hypertension,smoking,and high level of low-density lipoprotein,HDAC9 gene polymorphisms may be associated with the LAA type IS. rs2107595 locus CT + TT genotype may be an independent risk factor for LAA type IS.
6.Research progress of genes of cerebral palsy
Xinyang ZHANG ; Zhimei JIANG ; Zhihai LYU ; Wei PANG ; Jin GUO
Chinese Journal of Applied Clinical Pediatrics 2015;30(23):1835-1837
Cerebral palsy (CP) is the most common activity limitition of children, their movement and posture impairments persist throughout whole life.In recent years, CP has been significantly increasing with the improved survival rate of newborns.This may lead to a great burden and costs to both the family and the society.A variety of risk factors have been proposed to be associated with CP.However, recent abroad researches indicate that genetic factors may predispose to CP of newborns and initial results of related researches infer that several susceptibility genes may contribute to CP's development, masqueraders have a great impact on CP's clinical symptoms.Now, the recent publications related to virulence genes and masqueraders of CP are reviewed.
7.Individual FKS06 administration in liver transplantation
Yingfie LU ; Jinyan ZHANG ; Zhaowen WANG ; Zhihai PENG
International Journal of Surgery 2008;35(8):528-531
Tacrolimus, an immunosuppressant used after liver transplantation, has a narrow therapeutic range and its pharmacokinetic variability complicates its daily dose assessment. Therapeutic drug monitoring after liver transplantation, which is the mainstay to clinical immue suppression adjustment, still has its short- age. The development of pharmacogenomics depends on genetic polymorphism provides more reasonable di- rections to individualized immunosuppressive therapy. At present, the researches of the therapy mainly focus on the genetic polymorphism of coding gene of P450, PgP and nuclear receptor.
8.Clinical analysis of 35 cases with perloperafive pulmonary embolism
Zhihai GAO ; Guoxiang GE ; Xiaomei ZHANG ; Changzhu LIU
Chinese Journal of General Practitioners 2008;7(5):341-343
Thirty-five patients with perioperative pulmonary embolism during January 2000 and October 2006 were analyzed retrospectively.Disease diagnosis and treatment outcomes were assessed.Of the 35 patients,2 died without any management,and 33 received conventional anti-coagulation and thrombolysis.including 3 percutaneous catheterization.Disease improvement Was seen in 29.Four deaths occurred after no response to the treatment.Thus,early diagnosis and treatment,which miight depend on further understanding of pulmonary embolism,could play an important role in reduced adverse events.Prevention is crucial to avoid perioperative occurrence of pulmonary embolism.
9.Inhibitory effects of ventilation with various tidal volumes on inflammatory responses of rabbits with acute lung injury induced by seawater drowning
Yuhong LIU ; Zhihai HAN ; Yunyou DUAN ; Zhicheng ZHANG ; Huasong FENG
Medical Journal of Chinese People's Liberation Army 2001;0(07):-
0.05). After ventilation with 6ml/kg tidal volumes, the TNF-? in alveolar lavage fluid (1.58?0.56pg/ml) was significantly lower in b1 group compared with that in control group (1.73?0.81pg/ml, P
10.Polymorphisms in The 5' flanking Region of IGF-Ⅰ Gene are Associated With Cashmere Fibre Traits in Liaoning Cashmere Goats
Cuihua GUO ; Cunling JIA ; Wei ZHANG ; Xiaoping ZHU ; Zhihai JIA
Progress in Biochemistry and Biophysics 2006;0(05):-
Insulin-like growth factorⅠ(IGF-Ⅰ) is essential for the growth and differentiation of hair follicles which is an important part of wool and cashmere.But there is no report on polymorphisms of the IGF-Ⅰ gene in cashmere goat,and also few candidate genes for cashmere production traits have been reported in cashmere goats.The objectives of this work were to detect the single nucleotide polymorphism(SNP) in the 5' flanking region of IGF-Ⅰ gene and to determine their association with fibre traits in Liaoning cashmere goats.The fibre traits data investigated in the experiment were combed cashmere weight,cashmere fibre length and cashmere fibre diameter.A few individuals of the Liaoning cashmere goats,selected according to phenotypic character,were used for SNPs detection in the 5'flanking region of IGF-Ⅰ gene,and four point mutation G→C(388 bp),A→G(668 bp),A→ C(719 bp),G→A(752 bp) were identified,which result in a CdxA bonding-site lack,and score increase of C/EBP site in the 5' flanking region compared with the wild type.Then different genotypes were detected in 520 Liaoning cashmere goats using create restriction site(CRS) and polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP),three genotypes,AA,AB and BB were observed coded for by two different alleles A and B for each SNP,and 13 diplotypes were identified in the groups of the four SNPs.The relationships between the genotypes and combed cashmere weight,cashmere fibre length and cashmere fibre diameter were analyzed.The statistical analysis showed that animals with the genotype AA of SNP2 in Liaoning cashmere goats had the thinnest cashmere fiber diameter compared with those with the AB and BB genotype(P