Colorectal cancer is induced by multiple factors.Multiple genes should be considered for the research of colorectal cancer.Single nucleotide polymorphisms,a new tumor gene researching method,play a key role in colorectal cancer researching.Finding colorectal cancer special SNP is vital for selecting high risk population and predicting the risk of developing colorectal cancer.

OBJECTIVE:To investigate the relationship between the variation of bacterial-resistant and the consumption of antibacterial drugs. METHODS:The DDDs and its sequence of antibacterial drugs in 70 hospitals in Shanxi province over 3 years and the synchronic annual resistant rates of some pathogenic bacteria were analyzed statistically and the correlation between the variation of bacterial-resistant and the drug consumption was analyzed. RESULTS:In terms of variety,there was no obvious change for the top twenty antibacterial drugs. The antibacterial drugs with high DDDs thus ranked the lead were those kinds that with high bacterial drug resistance. At a value of over 50%,the bacterial drug resistance rate would not decrease obviously with the variation of the drug consumption. The drug resistance rate increased greatly with the increase of the consumption of the forth generation of cephalosporins cefepime. The consumption of vancomycin increased,but its resistance rate decreased. The antibacterial compound preparation showed satisfactory antibacterial activity even if its consumption had a marked increase. CONCLUSION:There is a direct relation between the consumption of antibacterials in large quantity and the variation of drug resistance. It is necessary to strengthen clinical management on antibacterials to raise rational drug use level.

Objective: To observe the therapeutical effect of intermittent positive pressure ventilation (IPPV) on pulmonary edema after seawater drowning in rabbits.Methods:Fourteen seawater lavage induced pulmonary edema New Zealand rabbits models were assigned to receive IPPV treatment.Blood gas analysis, pulmodynamics, hemodynamics status and serum tumor necrosis factor(TNF ?) were monitored at various time points.After 3 h ventilation rabbits of each group were killed to gain lung tissues for pathology examination, and lung lavage fluid was analyzed to count leukocytes.Results:PaO 2 significantly increased after the use of ventilation in pulmonary edema rabbits models ( P

OBJECTIVE:To evaluate the status quo and the tendency of the use of narcotic analgesics in patients with cancer in our hospital.METHODS:The use of narcotic analgesics in the inpatients with cancer between 2005 and 2008 in our hospital was analyzed statistically in respect of the consumption sum,number of patients using this drug and the prescriptions etc.RESULTS:Morphine Sulfate Controlled release Tablets(10 mg) was not welcome by both physicians and patients; however,the consumption sum and DDDs of Fentanyl transdermal patches and Morphine Sulfate Controlled-release Tablets (30 mg) had been dominated the front places over the 4 years.The consumption quantity of common Morphine tablets kept steady increase yet no obvious change has been noted in average dose per prescription;however,the per capita consumption quantity has been increasing.Bucinnazine hydrochloride tables as an two step analgesics kept decreasing in consumption.The total consumption of Morphine injection and Bucinnazine injection were decreasing on the whole.CONCLUSION:The use of narcotic analgesics for patients with cancer in our hospital is in line with the principle of cancer pain three-step analgesic ladder recommended by WHO.

Objective To evaluate the efficacy of ultrasound-guided combined C5 and superficial cervical plexus block in patients undergoing clavicle surgery.Methods Sixty ASA physical status Ⅰ or Ⅱ patients,scheduled for elective clavicle surgery,were randomly divided into 2 groups (n =30 each) using a random number table:injection with 10 ml local anesthetic guided by ultrasound group (group U),and injection with 10 ml local anesthetic guided by anatomical landmark group (group A).A mixture of 0.375 ％ levobupivacaine and 1.0％ lidocaine was used.The time spent performing the block onset time of analgesia,onset time of analgesia in the medial border,midpoint and lateral border of the clavicle and duration of analgesia were measured.The effectiveness of block (excellent,good,failure) was assessed.The complications were observed.Results Compared with group A,the time spent performing the block was significantly prolonged,the onset time of analgesia in the lateral border and midpoint of the clavicle was shortened,the rate of excellent anesthesia was increased,and the duration of analgesia was prolonged in group U (P ＜ 0.05).No complications occurred in group U,while vascular puncture occurred in 3 cases,and one patient developed mild toxic reaction in group A.Conclusion Ultrasound-guided combined C5 and superficial cervical plexus block provides better block,with faster onset time of analgesia in the lateral border and midpoint of the clavicle,longer duration of analgesia and fewer complications as compared with that guided by anatomical landmarks in patients undergoing clavicle surgery.

Tacrolimus, an immunosuppressant used after liver transplantation, has a narrow therapeutic range and its pharmacokinetic variability complicates its daily dose assessment. Therapeutic drug monitoring after liver transplantation, which is the mainstay to clinical immue suppression adjustment, still has its short- age. The development of pharmacogenomics depends on genetic polymorphism provides more reasonable di- rections to individualized immunosuppressive therapy. At present, the researches of the therapy mainly focus on the genetic polymorphism of coding gene of P450, PgP and nuclear receptor.

Objective In previous study,we carried out refined mapping of loss of heterzygosity (LOH) on 1q31.1-32.1 and found that a minimal region of frequent deletion was located at DIS413-D1S2622,which indicated that the region could harbor a tumor suppressor gene associated with colorectal carcinoma.This study was to screen for the potential tumor suppressor gene (TSG) on D1S413-D1S2622 in Chinese origin patients with sporadic colorectal cancer.Methods 25 genes located in the D1S413-D1S2622 region were chosen and a microarray-based high throughput screening conducted in 19 sporadic colorectal cancers to identify candidate tumor suppressor genes.The relationship between expression levels of candidate genes and the clinicopathological data was analyzed.Real-time PCR was performed to validate the microarray results.Results According to the microarray-based high throughput screening,we found 4 significantly down-expressed genes,including CSRP1,LMOD1,PPP1R12B and CFHL3.There was no significant association between of CFHL3,CSRP1,LMOD1,PPP1R12B expression and the clinicopathological data.CSRP1 could be a colorectal cancer related tumor suppressor gene.CSRP1 was down-regulated in colorectal cancer.Conclusions CSRP1 might be involved in the progression of colorectal cancer.

Objective To study the reliability and validity of the Chinese version of the dynamic occupational therapy cognitive assessment for children (DOTCA-Ch).Methods The English version of the DOTCA-Ch was translated into Chinese. One hundred and nine students from the city of Jiamusi were assessed with the Chinese Binet intelligence test and the DOTCA-Ch. Intra-class correlation coefficients (ICCs) were used in inter-rater and intra-rater reliability assessments. Cronbach's alpha coefficient was used to assess internal consistency. Factor analysis was used to assess construct validity. Pearson correlation coefficients were used in assessing criterion-related validity. Results The total ICC of the DOTCA-Ch was 0. 892. Cronbach's alpha coefficients for the subtests were orientation 0. 713 ; spatial perception 0. 793 ; praxis 0. 850 ; visuomotor construction 0. 844 and thinking operations 0. 630. The ICC for intra-rater reliability was 0. 991 and for inter-rater reliability it was 0. 989. Using the Binet intelligence test as a benchmark, the Pearson correlation coefficient between the IQ values and the total DOTCA-Ch scores was 0. 872 for 6 year-olds, but 0. 870 for 11 and 12 yearolds. Factor analysis revealed an acceptable set of 5 common factors. Their cumulative contribution was 67％.Conclusions The Chinese version of the DOTCA-Ch is reliable and valid. It can be used as an effective measuring tool to evaluate children's cognitive functioning.

Objective To evaluate the clinical significance of combined serum tumor markers assay in patients with gastric or colorectal carcinoma. Methods Serum level of 12 common tumor markers, including CA19-9,NSE,CEA,CA242 Jerritin,Beta-HCG,AFP,free-PSA,PSA,CA125,HGH and CA153, was measured with multi-tumor markers protein biochip detective system in 179 cases of gastric and colorectal carcinoma, 82 patients with benign digestive disease and 160 healthy volunteers. Results Cancer patients had significantly higher positive rates than that of two other controls (P

Objective To investigate the correlation between histone deacetylase 9 gene ( HDAC9 ) single nucleotide polymorphisms and ischemic stroke ( IS) subtypes. Methods A total of 202 patients with IS were enrolled prospectively. According to the Trial of Org 10172 in Acute Stroke Treatment ( TOAST) classification,the patients with IS were divided into large artery atherosclerotic stroke group (LAA,n=149) and the small-artery occlusive stroke group (SAO,n=53). A total of 201 age- and sex-matched healthy subjects over the same period were enrolled as the control group. Polymerase chain reaction-ligase detection reaction ( PCR-LDR ) was used to perform genotyping for HDAC9 gene rs11984041 and rs2107595 loci in all subjects. The correlation between genotype and IS was analyzed with multivariate logistic regression analysis. Results ( 1 ) In all the subjects detected, the genotype detected from rs11984041 loci was type CC,and 3 genotypes including CC,CT,and TT were detected from rs2107595 loci. (2) There were significant differences in dominance model (CT+TT,CC) genotypes and allele frequencies among the LAA, SAO, and control group dominance model ( CT +TT, CC ) and (χ2 =8. 635,P=0. 013, χ2 =10. 309,P=0. 006);and there were no significant differences in dominance model (CT+TT,CC) and allele frequencies among the 3 groups (after adjustment all P>0. 017). Compared with the control group,there were significant differences in the CC,CT,and TT genotypes and allele frequencies in the LAA group (χ2 =7. 446;P=0. 006). In the dominance models (CT+TT),there was significant difference in genotype frequencies between the LAA group ( 65. 1%,97/149 ) and the control group (52. 2%,105/201) (χ2 =5. 800;P=0. 016). (3) Multivariate logistic regression analysis showed that in addition to gender,hypertension,smoking,and high level of low-density lipoprotein,the dominance model CT+TT genotype was associated with the LAA type IS ( CT+TT genotype:OR,1. 909,95% CI 1. 055-3. 454,P =0. 033). Conclusion In addition to the risk factors for cerebrovascular disease such as gender,hypertension,smoking,and high level of low-density lipoprotein,HDAC9 gene polymorphisms may be associated with the LAA type IS. rs2107595 locus CT + TT genotype may be an independent risk factor for LAA type IS.