T-type calcium channels present in cardiovascular, neuronal and endocrine systems, and they are now receiving attention as novel therapeutic targets. It plays important roles in multiple cellular functions and genes those encode the T-type calcium channels have been recently reported. Many drugs and compounds non-specifically block T-type calcium channels. We review circumstances of the research of T- type calcium channels in the molecular structure, distribution, function, regulation and the related drugs.

Objective Nowadays,with the development of medical vehicle,there are many parameters for inspection in its interior.It has disadvantages,such as complex routing and low system reliability,to use DCS.CAN bus technology can decrease routing and enhance system reliability.Methods OPC technology was used.A temperature supervisor control program and a database application were designed by VB,and the temperature was control by the fuzzy control methods.Results The temperature supervisor control system was established with CAN bus to explore the application of CAN bus in the medical vehicle.Conclusion It can enhance the system's reliability to use CAN bus technology in the medical vehicle.

Objective To promote worldwide standardization of methods to determine creatinine by tracing to reference measurement for achieving universal accuracy measurement of creatinine.Methods The results of external laboratory assessment (EQA) survey in China and CAP were compared and analyzed. Results There was significant difference among different measurement methods and assay systems.Conclusion It should promote worldwide standardization of determine creatinine, which makes it trace to reference measurement liquid chromatography-isotope dilution mass spectrometry (LC-IDMS).

[Objective]To analyze the reason for osteofascial compartment syndrome caused by tardive massive bleeding and to discuss the first-aid measure to treat continuing bleeding in operation.[Method]There were 5 cases in this study.Two osteofascial compartment syndrome with tardive haematoma were caused by secondary disturbances of blood coagulation.One iliopsoas muscle haematoma was caused by over-dose of warfarin(WARF).Vitamine K was applied to anti-warfarin and depressed haematoma by ultrasonic guiding puncturation aider a pause of warfarin.The other one suffered from continuing bleeding after a depression operation on right femoral bone haematoma.The bleeding could not be stopped until carbasus obturation and a supplementary of lood coagulation factor.The carbasus were taken out 36 hours later.Three traumatic pseudoaneurysm rupture happened to superior gluteal artec,arteriae tibialis posterior and deep femoral artery separately.The bleeding artery was ligated and removed the body of the blood tumor in these three cases.In addition,arterial embolism was applied in two of them before operation.[Result]No recurrence happened to the case with iliopsoas muscle haematoma after 16 weeks as a result of absorption and degeneration.As the patient with right femoral bone haematoma referred,the bleeding stopped after treated by carbasus obturation.The wound was closed 36 hours after the operation.A satisfactory sensory recovery,pulsation of dorsal pedal artery and arteria tibialis posterior were found.Rebleeding was found in 3 weeks after the wound closed.The pathobiology inspection of bone and muscle tissue around the fracture site showed osteoma sarcomatosum in right femoral bone.The author gave the patient to the operation of high amputation.No rebleeding,nervous symptoms caused by compression,limbs necrosis and ischemic contracture was found after 3 patients suffering from traumatic pseudoaneurysm rapture with followed-up from 8 month to 3 years.[Conclusion]Hemorrhea caused by secondary disturbances of blood coagulation or pseudoaneurysm can lead to osteofascial compartment syndrome.Embolism of the bleeding artery before removal of the aneurysm and haematoma can reduce bleeding in operation.It is the best choice for the doctor to stop operation immediately and stop bleeding by effective carbasus obturation.

The record of speech-language abnormalities scattered in some chapters of two classics of traditional Chinese medicine, Huang Di Nei Jing and Zhong Zang Jing. The descriptive modes of these abnormalities in Huang Di Nei Jing were more diversified than in Zhong Zang Jing. In aspect of clinical values, speech-language abnormalities in Huang Di Nei Jing were used to guide clinical diagnosis and treatment, such as describing body constitution and clinical mistreatments, but rare in Zhong Zang Jing.

Objective: To investigate the characteristics of the cultured cervical cancer cell line transfected with anti HPV16E6 ribozyme, and to investigate the possibility and practicality of ribozyme in treatment of cervical cancer. Methods: The anti HPV16E6 ribozyme and empty eucaryotic expressing plasmids were transfected by lipofectin transfection into CaSKi cell, which named as CaSKi R, CaSKi P respectively. The morphology and the soft agar forming ability were studied. The expression of E6, PCNA and C erbB 2 genes was studied through Flow Cytometry. The tumorgenicity of each cell was detected by injecting cells into the nude mice skin. Three groups of nude mice were injected by CaSKi, CaSKi R and CaSKi P cell separately. Another group of mice was injected by CaSKi cell on right side and CaSKi R cell on left side. Results: There is no distinct difference of the morphology and growth rate between CaSKi and CaSKi P, but the growth rate of CaSKi R decreased. The soft agar forming rate of CaSKi P was similar with that of CaSKi cells, while that of CaSKi R was found decreased. The result of flow cytometric analysis showed that anti HPV16E6 ribozyme could reduce the expression of E6, PCNA and C erbB 2 genes on CaSKi R cells, while this phenomenon was not found on the CaSKi P cells. The tumorgenicity of CaSKi R in nude mice was decreased compared with CaSKi and CaSKi P cells. Conclusion: Anti HPVE6 rivozyme could partly reverse the malignant phenotypes of CaSKi cells. The reason may be the decrease of E6 gene expression, and the succeeding decrease of the PCNA and C erbB 2 genes′ expression.

Transformers are required to demonstrate the ability to withstand short circuit currents. Over currents caused by short circuit can give rise to windings deformation. In this paper, a novel method is proposed to monitor the state of transformer windings, which is achieved through on-line detecting the leakage inductance of the windings. Specifically, the mathematical model is established for on-line identifying the leakage inductance of the windings by applying least square algorithm (LSA) to the equivalent circuit equations. The effect of measurement and model inaccuracy on the identification error is analyzed, and the corrected model is also given to decrease these adverse effect on the results. Finally, dynamic test is carried out to verify our method. The test results clearly show that our method is very accurate even under the fluctuation of load or power factor. Therefore, our method can be effectively used to on-line detect the windings deformation.

Precision controls monitor assay random error (reproducibility),accuracy controls assess total error,both random error and systematic error,and trueness (bias)of an assay represents systematic error.A trueness control should be metro-logical traceable,ideally with a target value determined by use of a reference material or a reference method,without metro-logical traceable to a reference measurement system,patient test results for the same patient from different laboratories may not be comparable.A trueness control should be commutable and its analytical response to a reference method and a routine field method should be equivalent to that of a patient sample.And trueness control values should generally be set at medical decision levels and be prepared by the providers of reference materials and manufactured in the same fashion as a calibrator.

Objective To explore the role of intracranial electrode EEG monitoring for localizing epileptogenic zone in epilepsy.Methods 20 patients with refractory temporal lobe epilepsy,whose seizure onsets were unable to be localized by MRI、routine EEG and clinical characteristic,accepted long-term intracranial electrode EEG monitoring.Bitemporal lobe subdural implanted trip electrode were used to perform the ictal and interictal EEG monitoring under the guiding of the stereotactic technique.The results routine of EEG,MRI and intracranial electrode EEG were combined.The accuracy for localization of epilepitogenic zone was evaluated based on the followed-up.Results The intracranial electrode EEG monitoring was implanted within 1～5 d.Each patient was recorded the ictal and interictal discharge and was detected clinical onsets at least twice.All patients could be localized by ictal EEG.The consistency between interical EEG and ictal EEG were observated in 15 cases,the inconsistency in 3 cases,and 2 cases were bilateral discharge in interictal.The clinical outcome showed that patients were seizure-free in 13 cases(65%),improved significantly in 3 cases(15%),improved in 3 cases(15%),unimproved in 1 case(5%)according to Engel's criteria after surgery.No significant complication was found in all patients.Conclusion The intracranial electrode EEG monitoring can localize epileptogenic zone and offer reliable evidence for epilepsy surgery.

Objective To investigate the influence of HLA immunogenic mismatching (IM) on acute rejection of renal transplants.Methods The function recovery time of renal allograft and the rate of acute rejection of 196 cases after cadaveric renal transplantation wereanalyzed. Results IM of HLA locus did not influence the function recovery time of the renal allograft. The IM of HLA-A locus did not increase the rate of allograft acute rejection whereas the HLA-B locus did and the HLA-DR increased the acute rejection rate significantly. Conclusion IM should not be ignored in HLA typing. HLA-B locus is related to the allograft acute rejection, while the IM of DR locus increasesthe allograft acute rejection rate significantly.