AIM: To investigate the effect of Dendranthema morifolium (Ramat) Tzvel (DM) on isolated rat heart and ventricular myocytes during ischemia/anoxia and reperfusion/reoxygenation.METHODS: The Langendorff perfused rat hearts were used to measure intraventricular pressure and coronary flow. The cell contraction and intracellular calcium transient in enzymatically isolated ventricular myocytes were determined. RESULTS: (1) DM (0.5 g/L) significantly attenuated the inhibitory effects induced by ischemia/reperfusion on left ventricular developed pressure (LVDP), ?dp/dt max, coronary flow and LVDP?HR, meanwhile increased the content of SOD and decreased the content of MDA in the myocardium; (2) DM (0.5 g/L) attenuated the inhibitory effects of anoxia and reoxygenation on [Ca 2+]i transient and cell contraction in isolated ventricular myocytes. CONCLUSION: DM attenuated the effects on contractility and intracellular calcium induced by ischemia/anoxia and reperfusion/reoxygenation in the isolated rat heart and the ventricular myocytes. The mechanism might be related to increase in SOD activity and maintaining [Ca 2+]i homeostasis.

Liquid Chromatography-Mass Spectrometry ( LC-MS) is becoming more and more widely used in clinical laboratory ,and LC-MS related quality management requires more rigid than it was .According to CLSI C62-A,this article provides a reference for the validation procedure of LC-MS assay, including limit of detection and lower limit of the measuring interval , linearity and dilution, imprecision, assay interferences and trueness.

Objective To evaluate the possible white matter damage and to define the location of the damage in Alzheimer′s disease (AD) with diffusion tensor imaging (DTI). Methods Twenty-four AD patients and twenty-one age-matched healthy volunteers received conventional and DTI scanning.The ADC and FA of white matter in temporal, parietal, frontal lobe and cingulum were measured respectively and the data underwent postprocessing.Results FA value of the whiter matter in frontal, parietal, temporal and cingulum in AD patients was 0.37?0.06, 0.32?0.05, 0.26?0.03, and 0.47?0.09, respectively, and ADC value was 9.50 e-10?2.02 e-10, 10.55 e-10?1.43 e-10, 11.45 e-10?0.76 e-10, and 10.10 e-10?2.18 e-10, respectively;FA value of the same corresponding regions in control was 0.44?0.06, 0.38?0.05, 0.32?0.05, and 0.56?0.06, respectively, and ADC value was 8.75 e-10? 1.63 e-10, 9.83 e-10?0.99 e-10, 11.13 e-10?0.78 e-10, and 8.28 e-10?1.65 e-10, respectively.FA value of the whiter matter in frontal, parietal, temporal, lobe and cingulum decreased (P≤0.006) and ADC value increased in cingulum white matter (P≤0.006) in AD patients.Conclusion DTI could reveal the damage in white matter of frontal, temporal, parietal lobe and cingulum.It suggested that not only the gray matter is injuried, but also the white matter is abnormal in AD patients.

AIM: To investigate the effect of interleukin-2 (IL-2) on the intracellular calcium in electrically stimulated adult rat ventricular myocytes during anoxia and reoxygenation. METHODS: The isolated cardiac ventricular myocytes were exposed to 5 min anoxia followed by 10 min reoxygenation. Chemical anoxia was introduced by Krebs-Henseleit (K-H) solution containing 10 -3 mol/L sodium dithionite. The spectrofluorometric method was used to verify intracellular calcium transient with fura-2/AM as calcium fluorescence probe. RESULTS: It was shown that during anoxia, the amplitude of Ca 2+ transient was decreased, diastolic [Ca 2+ ] i, time to peak and time to relaxation of Ca 2+ transient were increased. All the parameters were got back but did not returned to the pre-anoxia level during reoxygenation. IL-2 at 2?10 5 U/L administrated during anoxia aggravated the effect of rexoxygenation on [Ca 2+ ] i transient. Pretreatment with a specific ? opioid antagonist, nor-BNI (10 -8 mol/L), abolished the effect induced by IL-2 during anoxia on the [Ca 2+ ] i transients, whereas specific ? opioid antagonist, naltrindole (10 -6 mol/L), did not cancel the effect. CONCLUSION: It is concluded that administration of IL-2 during anoxia aggravated the effect of reoxygenation on the [Ca 2+ ] i transients of isolated ventricular myocytes, which was mediated by cardiac ? opioid receptor pathway.

AIM: To investigate the protective effect of auricularia auricular polysaccharide (AAP) on the myocardial injury induced by ischemia and its underlying mechanism. METHODS:AAP was orally administrated to rats at the does of 50, 100 or 200 mg·kg~(-1)·d~(-1) for 20 d. Myocardial injury was induced in anesthetized Sprague - Dawley rats by left anterior descending coronary artery ligation. Myocardial infarct size, the level of lactate dehydrogenase (LDH), the activity of superoxide dismutase (SOD) , the production of lipid peroxidation malondialdehyde (MDA) and protein level of myocardial collagen of the heart were measured. RESULTS: The average myocardial infarct size in AAP groups was significantly smaller than that in ischemia group. The level of serum LDH induced by regional myocardial ischemia was significantly decreased in AAP group compared to ischemia group ( P < 0.01). AAP inhibited the production of MDA and increased the activity of SOD. Furthermore, AAP reduced the protein level of myocardial collagen after ischemia (P < 0.01).CONCLUSION: AAP prevents myocardium from ischemia injury as an antioxidant.

AIM: To investigate the effects of auricularia auricular polysaccharide (AAP) on chronic cerebral ischemia injury in rats. METHODS: The chronic cerebral ischemia mode1 was made by permanent middle cerebral artery occlusion (MCAO) on the right side. AAP at different doses (50 mg/kg and 100 mg/kg) was intragastrically administered at the onset of ischemia and in the following days after operation, once a day for 4 weeks. After 4 weeks of MCAO, Morris water maze test was introduced to examine the learning and memory functions. Nissl staining was performed to detect the survival neurons in hippocampal slices. Level of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) in brain tissue were measured. RESULTS: Rats treated with AAP showed a shorter escaping latency in spacial navigation test because the AAP treated rats spent less time to find the platform in spatial probe test. More survival neurons in hippocampal slices were observed from AAP treated rats. Also, the MDA level in brain tissue was reduced and SOD activity in brain tissue was increased in the AAP treated rats with MCAO. CONCLUSION: AAP protects rats from chronic brain ischemic injury, in which its anti-oxidative effect might be involved.

Objective To study the effects of improving elderly patients with bowel dysfunction after cardiopulmonary bypass.Methods Choose 54 elderly patients with CPB were randomly divided into tree groups:the control group and the Gln group and the Combination group,every group has 18 patients.Glutamine(Gln) at 0.4 g/kg was administrated to the patients in the Gln group and the combination group during the beginning at CPB and two days after the operation.Based on the Gln group,the patients of The Combination group were given the different oral Chinese medicine before and after operation,according to syndrome differentiation classification.Plasma endotoxin,IL-6 and DAO were measured before operation and at the endof operation and 24 h,48 h after operation.At the same time,we used German Siemens Company's X3000 Color Doppler machine to test every group of patients superior mesenteric artery.We tested before surgery,and one to two days after operation,from the starting point within 2cmof the superior mesenteric artery and the angle between the ultrasound beam flow was less than 60 °,the probe frequency was 3.5 MHz.The detection of hemodynamic indexes were EDV and PI.Results After the test,the plasma endotoxin and IL-6 levels and DAO levels of the Gln group were reduced than the Control group after cardiopulmonary bypass.The plasma endotoxin and IL-6 levels and DAO levels of the Combined gnup were decreased than the Gln group after cardiopulmonary bypass and one day after operation.Analyze superior mesenteric artery diastolic velocity (EDV)and pulsatility index (PI) by the Color Doppler.After operation,the EDV in the Gln group and the Combined grup were significantly higher than the control group,the Pl were lower than the control group.The parameters levels have significant difference after cardiopulmonary bypass and one day after operation(P ＜ 0.05 ).The Combined group was improved than the Gln group after cardiopulmonary bypass and one day after operation.According to the traditional Chinese medicine diagnosis,there were eighteen patients in the Combined group,including fifteen patients in type of deficiency of both qi and yin and three patients in type of fu-viscera syndnome.All of the patients have defecated within tree days after operation.In the type of deficiency of both qi and yin nine patients were cured and six were shown to be effective after operation.All the patients in type of fuviscera syndrome were cured.Conclusion N(2)-L-Alanyle-L-Glutmaine have protective effect on the intestinal mucosal barrier after CPB.Combined traditional Chinese Medicine preparation was more conducive to the intestinal function recovery of elderly patients after cartiopulmonary bypass.

Objective To establish a large animal ( sheep) model to serve the experiments of domestic pulsatile ventricular assist device.Methods Three small-tail Han-sheep were anesthetized and the vein access and artery access were achieved.The cardiopulmonary bypass was established through left thoracotomy.Ventricular fibrillation was induced. An hole was made in the apex of left ventricle and the apex cannulation was sutured to it.The aortic cannulation was su-tured to the descending aorta.The two cannulations were connected to the domestic pulsatile ventricular assist device ( DP-VAD) and the driver was turned on.The working of DPVAD and the conditions of the animals were observed.Results The DPVAD worked well and uni-directional blood flow was driven by positive and negative pressure.The left ventricle was unloaded and the blood pressure was raised up.Conclusion The establishment of sheep model of pulsatile ventrieular as-sist device may play important role for the research and development of DPVAD in our country.

AIM: We examined the effect of interleukin- 2 (IL-2) on calcium handlin g of rat cardiomyocytes. METHODS: The effects of steady state an d transient chan ges in stimulus frequency on the intracellular calcium transient were investigat ed in the isolated ventricular myocytes with spectrofluorometry technique. RESULTS: Under the steady state (0.2 Hz), IL-2 at 2?10 5 U/L decr eased the peak [Ca 2+ ] i and amplitude of the [Ca 2+ ] i transient, increas ed the diastolic calcium level, and prolonged the decay of the calcium transient . At 1.25 mmol/L of extracellular [Ca 2+ ], when increasing the stimulus frequency from 0.2 to 1.0 Hz, diastolic calcium level and peak [Ca 2+ ] i as well as the amplitude of the transient were inc reased. The positive frequency relationship was blunted in the IL-2-treated myoc ytes and this was not normalized by increasing extracellular [Ca 2+ ] t o 2.5 mmol/L . The caffeine induced Ca 2+ release was increased with increase in stimu lus freq uency. IL-2 inhibited the frequency relationship of caffeine induced Ca 2+ releas e. The restitution was not different between control and IL-2 groups at the 1.25 mmol/L of extracellular [Ca 2+ ], which was slowed in IL-2-treated myo cytes when t he extracellular [Ca 2+ ] was increased to 2.5 mmol/L. CONCLUSIO NS: It is concluded that the blunted frequency response of IL-2-treated myocytes was resulted from the decrease in SR Ca 2+ release, which was related to depression of SR funct ion. Despite the evidence of depressed SR Ca 2+ uptake, the restitution o f ca lcium transient at 1.25 mmol/L of extracellular [Ca 2+ ] remains uncha nged, which maybe due to the increase in the Na +/Ca 2+ exchanger activi ty.

Objective: To study the influence of Centipede Acidic Protein(CAP) on the proliferation and collagen synthesis of cultured neonatal rat cardiac fibroblasts(CFb) induced by angiotensinⅡ(AngⅡ),and to explore the mechanisms of CAP on cardiac fibrosis.Methods: Neonatal rat cardiac fibroblasts were treated with AngⅡ to produce fibrosis model.The effects of CAP on proliferation of CFb were observed by MTT colorimetric assay,synthesis of collagen was observed by the hydroxyproline concentration.The NO contents were measured by Nitric acid reductase method.The c-myc expression was examined by semi-quantitative RT-PCR analysis.Results: Compared with that of control group,the proliferation,collagen synthesis and the levels of c-myc mRNA expression of CFb in the model group increased,while the NO contents decreased obviously(P