OBJECTIVE To find out the prevalent distribution and multidrug resistance trend of meticillin-resistant Staphylococcus(MRS) and to prevent fulminant prevalence of MRS and opt for effective therapeutic means.METHODS The bacterium was(identified) by the way of API Staph and the TH-16S′s coding tube.The(antimicrobial) susceptibility testing was adoped by ATB-STAPH5 and MRS was examined by dilution and K-B.All statistical analyses were performed using SPLM 3.0 software.RESULTS The isolation rate of meticillin-resistant S.aureus(MRSA) and meticillin-resistant coagulase negative Staphylococcus(MRCNS) was 38.09%,20.00%and 87.65%,89.00%,(respectively) in 2 years.Along with the age of patients,the infection from MRS was(increasing).The isolation rate of MRSA was 28-26%,but that of MRCNS was more than 80% from S.epidermidis,S.haemolyticus,S.hominis,and S.saprophyticus subsp saprophyticus.All parts of our body can be(infected) by MRS.The more than 30% MRSA were multidrug resistant and the approximately 11.87-13.75% MRCNS were also multidrug(resistant).(CONCLUSIONS) The isolation rate of MRSA from national surveillance(network) is not(different) with that of MRCNS.

Jinyingzi is the dry ripe fruit of Rosa leav gata Michx.. It contains mainly tannic acid, malic acid, citric acid, vitamin C and saponins. Its effect on urinary sy stem was studied on mouse Pollakiuria model by severing hypogastric nerve. Results showed that the water extract of R. laevigata can decrease the frequency and prolong the interval of urination and increase the quantity of eacn urination. The said extract can inhibit the automatic contraction of isolated rabbit jejunal smooth muscle and antagonize the spasmodic contraction arouscd by acetylcholine and barium chloride of isolated rabbit jejunal smoothmusele, and isolated vesical smooth muscle of mouse, and antagonize reaction of contractility aroused by noradrenalin on isolated thoracic aorta strips of rabbit. All of the the three inhibitions of smooth muscle contraction showed signicant dose-response curve.

Objective To compare the influence of endoscopic thyroidectomy via breast areola approach and traditional thyroidectomy on the immune function.Methods Fifty cases undergoing partial thyroidectomy were divided into endoscopy and open thyroidectomy groups(25 cases in each).Blood samples were obtained at 1 day before the surgery and 1,2,and 3 days postoperation to count the CD3,CD+4 and CD+8 T lymphocytes using flow cytometry,and determine the concentration of immunoglobulins(IgG,IgA,and IgM),CRP,and complements(C3 and C4)with immunonephelometry.Results In both the groups,the concentrations of CD3,CD+8 T lymphocytes,and complement C4 decreased significantly at 24 hours after the surgery(P0.05).Conclusions Both endoscopic and traditional thyroidectomy could depress the immune function,however no significant difference exists between the two methods in the level of depression.

Objective To understand the clinical features and laboratory examination and diagnosis of Langerhas cell histio-cytosis (LCH) in newborns. Methods Retrospective analysis of the clinical data of a neonatal case of LCH was performed. Results A one-day-old girl was admitted with chief complaint of eating less, less crying and skin rash for one day. The unequal sized rash was on the head, limbs, trunk, toe and oral mucosa. The skin rashes were protruding from the skin and were partially broken and crusted. Biopsy of the skin tissue showed the expression of S-100 and CD1α, consistent with the diagnosis of LCH. Conclusions When new-borns have special rash after birth, LCH should be considered. To avoid misdiagnosis, skin biopsy should be performed.

AIM To study the anti oxidation and cancer chemoprevention mechanism of procyanidins extracted from Vitis vinifera. METHODS A simple colorimetric method is used for the measurement of H 2O 2 produced by PMNs induced by croton oil. RESULTS It was shown that procyanidins could inhibit PMNs releasing H 2O 2 induced by croton oil. Procyanidins serum of rats also had the same effect. The effect was time and dose dependent. Inhibitory effect of procyanidins on hepatic mitochondria lipid peroxidation induced by croton oil was also observed. Procyanidins could raise the activity of SOD and decrease the level of MDA. CONCLUSION It was concluded that anti oxidation effect of procyanidins was one important mechanism of cancer chemoprevention.

Background and purpose:Cancer is a major public health issue in China and worldwide, which se-riously threatens human beings as well as social and economic development. This study explored the relationships between the cancer distribution characteristics and cancer prevalences in Chinese cancer surveillance regions to provide scientific evidence for cancer prevention and management.Methods:The data were obtained from the book named“Prevalence and Mortality of Cancer in China from 2003-2007” including incidence of 23 cancer types in 32 regions of China published by the Academy of Military Medical Sciences of the Chinese PLA in 2012. Correspondence analysis was used to gain the relation between the prevalence and area distribution. Cluster analysis was used to obtain the classifications with special significance by putting the cancers or regions with similar characteristics into a cluster.Results:Esophageal cancer, gastric cancer, liver cancer, lung cancer, colorectal and anal cancer have high incidence and mortality in both genders. The districts with high incidence of esophageal cancer and gastric cancer were grouped together. The counties or cities (Shexian, Yangcheng, Linzhou, Yanting, Yangzhong and Jianhu) with high incidence of esophageal cancer and gastric cancer were classified into same cluster frequently. Fusui was grouped along because of the lower incidence of various cancers than the national average except for liver cancer. Guangzhou, Sihui and Zhongshan were the districts with high incidence of nasopharyngeal carcinoma in both genders. Rural areas in Qidong and Haimen were classified into a cluster in male and total data for the high incidence of liver cancer. Colorectal cancer, anal cancer and breast cancer in women also had high incidence in urban areas. Cervical cancer had the second level high incidence in women following diseases of digestive system, breast cancer and lung cancer.Conclusion:Similar pathogenic factors may exist in counties or cities of Shexian, Cixian, Yangcheng,etc, because of the high prevalence of esophageal cancer. Similar pathogenic factors may also exist in other districts or cancers that were classified into the same cluster.

Traditional 2D cell culture technology is widely used in cell culture field, but it lacks the formation of 3D matrix scaffold and lacks specific cytokines in vivo.Cells in 3D cell culture system are similar to cells in vivo for gene expression and cell activity. The article focuses on the application of three-dimensional cell culture technology in cancer research, including the construction of tumor microenvironment,tumor biological behavior, tumor angiogenesis, drug resistance and so on and provides a reference for cancer research workers.

Objective To study the distribution patterns of bacterial flora in sigmoid colon tissues and stools in normal population.Method Bacterial flora were identified and analyzed by using 16sDNA sequencing technology in fresh stool samples (n =13) and colon mucosa samples (n =10).Results The diversity and abundance of bacterial flora were significantly larger in the stool samples than in the sigmoid colon samples (P ＜ 0.001,P ＜ 0.001,P =0.042,P =0.006).The consititution of phylum flora between the two groups were same,including flrmicutes,bacteroides,proteobacteria,and actinomycetes.However,the proportions of firmicutes and bacteroides in stool samples were significantly higher than in the sigmoid colon samples,whereas the proportion of proteobacteria was significantly lower (P ＜ 0.001,P =0.025,P ＜ 0.001).At the genus level,faecalibacterium and bacteroides were the dominant flora in feces,whereas pseudomonas,lactococcus,acinetobacter,and flavobacterium were the most common flora in sigmoid colon mucosa.The amounts of bifidobacterium and lactobacillus were low in both two groups.Conclusion The distribution of bacterial flora remarkably differ in stools and sigmoid colon mucosa.

AIM To observed the effect of procyanidins on DNA damage induced by carcinogen or hydroxyl radical (OH?),and inhibitory effect on topoisomerase. METHODS Using labeled TdR uptake assay and single cell gel electrophonesis. Topoisomerase was extracted from B16F10 cell lines, used agarose gel electrophoresis assay. RESULTS It was showed that hydroxyl radical (OH?) or different doses of MNNG can induce severe DNA damage of L 929 cell lines. Procyanidins can alleviate the damage induced by MNNG or OH?. It shows certain dose dependent effect relationship . But there is no significant inhibitory against Topo Ⅱ activity by procyanidins even at a dose of 50 ?mol?L -1 . CONCLUSION Procyanidins from grapes seeds can protect DNA damage,but not inhibit topoisomerase activity. This is one of cancer chemoprevention mechanism of Procyanidins.

Objective To observe the preventive effects of puerarinon development of deep vein thrombosis (DVT) in elderly women with osteoporosis after hip replacement. Methods 100 elderly women with osteoporosis who scheduled for femoral head arthrolastybetween January 2012 and January 2014 in Cangzhou Central Hospital were selected and then randomly divided into a study group (n=50) and a control group (n=50). The control group received routine anti?inflammatory and anticoagulant therapy ,while the study group received intravenous drip of puerarinof 400 mg mixed with 5%glucose liquid 500 ml daily for 15 days as a treatment course. After the treatment, DVT incidence rate ,change of early diagnostic indexes and hemorheology were compared with their baselines. Results DVT incidence rate was significantly lowerin the study group than in the control group (8.0%vs. 24.0%, P<0.05). Ascompare with the control group,thromboxane A2/prostacyclin,plasma homocysteine and hemorheology?were significantly reduced (P < 0.05). Conclusions Puerarin has a preventive effect for deep vein thrombosis in elderly women with osteoporosis after hip replacement.