OBJECTIVE:To study the enhancing effects of azone at different concentrations on percutaneous permeation of compound tinidazole gel and to find out the best concentration.METHODS:Using vertical Franz-type diffusion cell and dif?ferent concentrations of azone as enhancer,the concentrations of tinidazole,chloramphenicol and salicylic acid in permeation solution were measured by RP-HPLC,then cumulative permeation quantity(Q)was calculated.RESULTS&CONCLUSION:Azone at different concentrations could promote the permeability of3ingredients of compound tinidazole gel,and2%azone had the most obvious permeation-enhancing effect.

AIM: To establish a HPLC method for the determination of tanshinone Ⅱ A in Compound Danguifukang Capsule. METHODS: Analytic column: Reliasil C18(5μm, 250mm×4.6mm), protective column: (5μm, 12.50mm×4.6mm); mobile phase: methnaol-ultrapure water (80: 20); detective wavelength was at 270a good linearity within the range of 1×10-2-0.16μg, r=0.9995. The average recovery was 99.73% and RSD was 2.91%. CONCLUSION: The method is feasible, and simple to operate and suitable for the quality control of Compound Dangguifukang Capsule.

OBJECTIVE:To establish the quality control method of angelica capsule.METHODS:The chief components of the preparation,radix angelica sinensis,radix salvia miltiorrhizae,rhizoma corydalis,radix astragali,which were identified by TLC;the content of ferulic acid in angelica and tanshinoneⅡ A in Salvia were determined by HPLC method.RESULTS:The TLC(thin layer chromatogram)spots were clear;the linearity of ferulic acid was within the range of0.00255?g～0.0816?g(r=0.9997)and the linearity of tanshinoneⅡ A was within the range of0.01?g～0.06?g(r=0.9995).CONCLUSION:Th_ is method is simple and highly reproductive,which can be used for the quality control of preparation.

AIM: To establish a HPLC method for the determination of tanshinone ⅡA in Compound Danguifukang Capsule. METHODS: Analytic column: Reliasil C 18 (5 ?m, 250 mm?4.6 mm), protective column:(5 ?m, 12.50 mm?4.6 mm); mobile phase: methnaol-ultrapure water (80∶20); detective wavelength was at 270 nm; temperature was at 30 ?C ; the flow rate was about 1.0 mL?min -1 . RESULTS: The standard curve showed a good linearity within the range of 1?10 -2 -0.16 ?g, r=0.999 5. The average recovery was 99.73% and RSD was 2.91% . CONCLUSION: The method is feasible, and simple to operate and suitable for the quality control of Compound Dangguifukang Capsule.

This article analyzed the universality of fatigue existing and the significance of quantization evaluation on fatigue.It expounded the recognition of fatigue in traditional Chinese medicine,and further more proposed the methods of quantization evaluation on fatigue.