Objective: To study the effects of fortified conjugated linoleic acid (CLA) milk on body fat and plasma lipids in mice.Method:Forty male Kunming mice of 4 w old were randomly divided into four groups and fed diet and milk supplemented with 0%,0.1%,0.5%,1.0% CLA (percent of diet, dry mass) respectively for 4 w.Weight gain,body fat,intra-abdominal fat,feed conversion efficiency and plasma TG, TC, HDL , LPL were measured.Results:At the end of the experiment, all these physiological indices were decreased with increasing CLA in the milk and were lower significantly than that of control group when supplemented with 0.5% CLA in milk. TG , TC and (TC- HDL) / HDL were decreased, but HDL and LPL were increased with increasing CLA amount in the milk. TG,HDL,LPL and (TC- HDL)/ HDL were best when fed the milk supplemented with 1.0% CLA,while TC was the lowest when fed the milk supplemented with 0.5% CLA . Conclusion:The fortified conjugated linoleic acid is effective in lowering body fat and blood lipids.

AIM: To establish a HPLC method for the determination of tanshinone Ⅱ A in Compound Danguifukang Capsule. METHODS: Analytic column: Reliasil C18(5μm, 250mm×4.6mm), protective column: (5μm, 12.50mm×4.6mm); mobile phase: methnaol-ultrapure water (80: 20); detective wavelength was at 270a good linearity within the range of 1×10-2-0.16μg, r=0.9995. The average recovery was 99.73% and RSD was 2.91%. CONCLUSION: The method is feasible, and simple to operate and suitable for the quality control of Compound Dangguifukang Capsule.

AIM: To establish a HPLC method for the determination of tanshinone ⅡA in Compound Danguifukang Capsule. METHODS: Analytic column: Reliasil C 18 (5 ?m, 250 mm?4.6 mm), protective column:(5 ?m, 12.50 mm?4.6 mm); mobile phase: methnaol-ultrapure water (80∶20); detective wavelength was at 270 nm; temperature was at 30 ?C ; the flow rate was about 1.0 mL?min -1 . RESULTS: The standard curve showed a good linearity within the range of 1?10 -2 -0.16 ?g, r=0.999 5. The average recovery was 99.73% and RSD was 2.91% . CONCLUSION: The method is feasible, and simple to operate and suitable for the quality control of Compound Dangguifukang Capsule.

Targeting the key questions in the scale development process of TCM syndrome, such as the definition of the concept, the construction of the theoretical framework, the quantitative classification of the items, the rational use of the statistical methods and so on, this article put forward that on the basis of carefully distinguishing the three concepts of syndrome diagnosis, syndrome evaluation and disease diagnosis, and based on TCM dialectical thinking and mathematical validation to build theoretical framework. A scientific and reasonable quantitative classification method was established based on the reliability and validity as indexes. Non-linear intelligent mathematical statistics and symptomatic index groups were used to analyze the ideas and methods of data mining, with a purpose to improve and perfect the methodology of the development of syndromes scale and to improve the establishment and application of the syndrome scale.

OBJECTIVE:To establish the quality control method of angelica capsule.METHODS:The chief components of the preparation,radix angelica sinensis,radix salvia miltiorrhizae,rhizoma corydalis,radix astragali,which were identified by TLC;the content of ferulic acid in angelica and tanshinoneⅡ A in Salvia were determined by HPLC method.RESULTS:The TLC(thin layer chromatogram)spots were clear;the linearity of ferulic acid was within the range of0.00255?g～0.0816?g(r=0.9997)and the linearity of tanshinoneⅡ A was within the range of0.01?g～0.06?g(r=0.9995).CONCLUSION:Th_ is method is simple and highly reproductive,which can be used for the quality control of preparation.

This article analyzed the universality of fatigue existing and the significance of quantization evaluation on fatigue.It expounded the recognition of fatigue in traditional Chinese medicine,and further more proposed the methods of quantization evaluation on fatigue.

Objective To observe the clinical safety of dual axis rotational coronary angiography (DARCA) in the diagnosis of coronary artery disease in Chinese population.Methods From March to December in 2010,74 patients undergoing diagnostic DARCA were enrolled.The improved isocentering technique was adopted in 34 of the patients at the end of the study during DARCA.Blood pressure,heart rate and symptoms were recorded immediately before-and-after contrast injections.Contrast dose,radiation exposure and procedure time for DARCA were recorded.Continuous variable data were analyzed using Student＇s t test,if normality assumption was violated,rank sum test would be used.Categorical variables were analyzed using x2 test.Results ( 1 ) Clinical safety:There was no chest pain documented during or immediately post-injection for all patients.Only 1 patient ( 1 ％ ) had an attack of ventricular tachycardia immediately after the contrast injection and then relieved automatically.Pre and post-injection systolic blood pressure values of left coronary artery were statistically different [ ( 116 ± 20 ) mm Hg vs.( 111 + 18) mm Hg( 1 mm Hg =0.133 kPa),t =3.303,P =0.001 ],and heart rates differed,too [ 73 ( 65- 84)bpm vs.71(64-78) bpm,Z =-4.789,P =0.001 ],but that imposed no clinical significance.(2)Contrast dose,radiation dose and procedure time:The mean contrast utilization,radiation dose and procedure time for DARCA were 28 (25-34) ml,8979 ( 6733-12 363 ) mGycm2 and 200 (164-270) s.Compared with conventional DARCA,improved isocentering technique during DARCA had less radiation exposure and procedure time in left coronary artery angiography and the whole coronary artery angiography [ left coronary artery angiographic radiation exposure:4004 (2932-5772) mGycm2 vs.5808 (4798- 8838) mGycm2,Z =-3.471,P =0.001 ;total radiation exposure:(8116 +2493) mGycm2 vs.( 11 371 ±4122) mGycm2,t =-4.176,P =0.001 ; left coronary artery angiographic procedure time:120 ( 80-180)s vs.150(126-214) s,Z =- 2.836,P =0.005; total procedure time:180 (139-240) s vs.220( 186-308 )s,Z =-3.004,P =0.003 ],but there was no statistically difference in contrast utilization [30(25-35) ml vs.27(25-34)ml,Z=-0.906,P=0.365].Conclusion This study demonstrates clinical safety of DARCA in the diagnosis of coronary artery disease in Chinese population.Compared with conventional isocentering technique of DARCA,improved isocentering technique can significantly reduce radiation exposure and procedure time on the basis of simplified operation,and replace the conventional isocentering technique,but randomized double-blind controlled studies should be conducted.

Objective To observe the effects of Di ao Xin xue Kang on connexin 43 ( Cx43 ) , in order to make clear the protective mechanism against ischemia -induced arrhythmias .Methods The rats were randomly divided into 3 groups according to the digital table:SM operation group(group SM,n=10),ischemia group(group IM,n=10),Di ao Xin xue Kang group(group DA,n=10).Myocardial infarction was induced by legation the left anterior descending coronary artery for 120 min.The expression of Cx43 of the ischemic myocardium was studied by immunohistochemistry technique .The ventricular arrhythmia was observed , and MDA, SOD levels were measured . Results Compared with the SM group ,the arrhythmia score[(3.8 ±0.7)points vs.(0.7 ±0.2)points,t=13.466, P<0.01]and serum MDA level[(8.3 ±0.8)nmol/mL vs.(4.2 ±0.7)nmol/mL,t=12.197,P<0.01]in the IM group were significantly increased,SOD activity in the IM group[(68.2 ±5.5)U/mL vs.(144.1 ±7.9)U/mL,t=24.932,P<0.01]decreased,and serum Cx43 content[(0.39 ±0.06) vs.(0.92 ±0.05),t=21.459,P<0.01] significantly decreased.Compared with IM group,the arrhythmia score of the DA group [(2.6 ±0.8) vs.(3.8 ± 0.7),t=3.570,P<0.01]decreased,and serum Cx43 content[(0.60 ±0.08) vs.(0.39 ±0.06),t=6.641,P<0.01]increased and distributed regularly.Conclusion Di ao Xin xue Kang can effectively alleviate Cx43 degrading induced by acute myocardial infarction ,the mechanism of this effect is associated with resisting oxidative stress .

Objective To study the effect of Zhibitai capsule on blood lipids,blood glucose and omentin in hyperlipidemic rats. Methods Thirty SPF male rats were randomly divided into control group,model group and Zhibitai group. The control group was fed with common diet,while the rest rats were fed with high - fat diet. After the model was successfully established,the Zhibitai group was orally administered 8mg/ kg Zhibitai capsule powder twice daily,the rats in the control group and the model group were given the same amount of normal saline respectively. After 4 weeks,the serum levels of total cholesterol (TC),triglyceride (TG),high density lipoprotein (HDL - C),low density lipoprotein (LDL - C),blood glucose and omentin were evaluated. Results Compared with the model group, the serum levels of TC[(3. 97 ± 0. 62) mmol/ L vs. (6. 33 ± 1. 10) mmol/ L,t = 5. 910,P < 0. 01],TG[(1. 51 ± 0. 47)mmol/ L vs. (2. 18 ± 0. 64)mmol/ L,t = 2. 472,P < 0. 05]and LDL - C[(0. 30 ± 0. 04)mmol/ L vs. (0. 48 ± 0. 16)mmol/ L,t = 3. 451,P < 0. 01)] of the Zhibitai group were significantly decreased,the HDL - C level was significantly increased[(1. 35 ± 0. 28) mmol/ L vs. (1. 06 ± 0. 31) mmol/ L,t = 2. 195,P < 0. 05],blood glucose decreased significantly[(5. 04 ±0. 82)mmol/ L vs. (6. 43 ± 1. 10)mmol/ L,t = 3. 204,P < 0. 01],and the concentration of omentin was significantly increased[(68. 59 ± 24. 82) ng/ L vs. (46. 86 ± 20. 12) ng/ L,t = 2. 151,P < 0. 05]. Conclusion Zhibitai capsule can effectively reduce the serum levels of TC,TG and LDL - C of hyperlipidemic rats and increase the content of HDL - C,effectively reduce blood sugar,and can increase the content of omentin.

Objective To study the effect of Zhibitai capsule on blood lipids,blood glucose and omentin in hyperlipidemic rats.Methods Thirty SPF male rats were randomly divided into control group, model group and Zhibitai group.The control group was fed with common diet,while the rest rats were fed with high－fat diet.After the model was successfully established,the Zhibitai group was orally administered 8mg/kg Zhibitai capsule powder twice daily,the rats in the control group and the model group were given the same amount of normal saline respectively. After 4 weeks,the serum levels of total cholesterol (TC),triglyceride (TG),high density lipoprotein (HDL－C),low density lipoprotein (LDL－C),blood glucose and omentin were evaluated.Results Compared with the model group, the serum levels of TC[(3.97 ± 0.62) mmol/L vs.(6.33 ± 1.10) mmol/L,t =5.910,P <0.01],TG[(1.51 ± 0.47)mmol/L vs.(2.18 ± 0.64)mmol/L,t=2.472,P<0.05]and LDL－C[(0.30 ± 0.04)mmol/L vs.(0.48 ± 0.16) mmol/L, t =3.451, P <0.01)] of the Zhibitai group were significantly decreased,the HDL －C level was significantly increased[(1.35 ± 0.28) mmol/L vs.(1.06 ± 0.31) mmol/L,t =2.195,P <0.05],blood glucose decreased significantly[(5.04 ± 0.82)mmol/L vs.(6.43 ± 1.10)mmol/L,t=3.204,P<0.01],and the concentration of omentin was significantly increased[(68.59 ± 24.82) ng/L vs.(46.86 ± 20.12) ng/L,t =2.151,P <0.05]. Conclusion Zhibitai capsule can effectively reduce the serum levels of TC,TG and LDL－C of hyperlipidemic rats and increase the content of HDL－C,effectively reduce blood sugar,and can increase the content of omentin.