Objective:To study the protective effect of glucagon-like peptide-1 (GLP-1) on the inflammatory damage induced by high glucose(HG) in placental trophoblasts HTR8/SVneo and its molecular mechanism.Methods:Trophoblasts HTR8/SVneo cells were cultured and divided into low glucose(LG) group treated with low glucose (5 mmol/L), HG group treated with high glucose (25 mmol/L), GLP-1 group treated with high glucose combined with GLP-1, miR-137+ GLP-1 group treated with high glucose combined with GLP-1 after the transfection of miR-137 mimic, miR-137 mimic group transfected with miR-137 mimic, negative control (NC) mimic group transfected with NC mimic, and miR-137 inhibitor group transfected with miR-137 inhibitor, NC inhibitor group transfected with NC inhibitor. Apoptotic rate, expression of miR-137 and IL-6 were measured.Results:The apoptotic rate and the expression levels of miR-137 and IL-6 in HG group were significantly higher than those in LG group. The apoptotic rate and the expression levels of miR-137 and IL-6 in GLP-1 group were significantly lower than those in HG group. The apoptotic rate and the expression levels of miR-137 and IL-6 in miR-137+ GLP-1 group were significantly higher than those in GLP-1 group. The apoptotic rate and the expression level of IL-6 in miR-137 mimic group were significantly higher than those of NC mimic group, the apoptotic rate and the expression level of IL-6 in miR-137 inhibitor group were significantly lower than those in the NC inhibitor group.Conclusion:GLP-1 is able to alleviate the inflammation injury of HTR8/SVneo induced by high glucose through the miR-137/IL-6 pathway.

Gastrointestinal neuroendocrine tumor(GI-NET)originates from peptide neurons and neu-roendocrine cells in gastrointestinal tract,and secrets peptide hormones,leading to carcinoid syndrome which rarely happens in clinical practice. Because of the improvement of diagnostic method and understanding of this rare disease,the morbidity is rising in recent years. The main treatments of GI-NET are surgery and compre-hensive therapy,consisting of chemotherapy and targeted therapy.

Objective To study the immunosuppression mechanism induced by ultraviolet (UV) and cis-urocanic acid. Method The auto lymphocyte proliferation test with Langerhans＇ cell (LC) in guinea -pig was performed. Results In exposure to low dose of UVB (25 J/m2) radiation, the inhibition rate of lymphocyte proliferation stimulated by LC was 10. 5%, the inhibition rates of UVB radiation in doses of 50 J/m2 and 100 J/m2 were 22.4% or 50%, respectively. The lymphocyte proliferation was almost completely suppressed by200J/m2 UVB radiation, while the inhibition of LC function by cis-urocanic acid was weak. Conclusion UVB significantly inhibits LC auto -stimulation in dose -dependent way, which may play an important role in UVB induced immunosuppression.

China ; Humans ; Industry ; Pneumoconiosis

Laboratory animal welfare is an important guarantee for the accuracy and reliability of experimental results, so it has been paid attention in scientific research .But the situation is not optimistic in the teaching practice .This paper has specially analyzed laboratory animal welfare and ethics in experiment teaching and provided specific measures involved in legislation , spread of animal welfare and implementation of 3R principle to ensure animal welfare .

Objective To study the immunosuppression mechanism induced by ultraviolet (UV) and cis urocanic acid. Method The auto lymphocyte proliferation test with Langerhans′ cell (LC) in guinea-pig was performed. Results In exposure to low dose of UVB (25 J/m2) radiation, the inhibition rate of lymphocyte proliferation stimulated by LC was 10.5％ , the inhibition rates of UVB radiation in doses of 50 J/m2 and 100 J/m2 were 22.4％ or 50％ , respectively. The lymphocyte proliferation was almost completely suppressed by 200 J/m2 UVB radiation, while the inhibition of LC function by cis urocanic acid was weak. Conclusion UVB significantly inhibits LC auto-stimulation in dose-dependent way, which may play an important role in UVB induced immunosuppression.

The teaching secretary of clinical department at medical university is required to be passionate,devoted,qualified and good at writing.The position is reserved for the person with healthy psychology,who knows well to deal with people,political training and sensitivity,advanced qualification.

The incidence of locally recurrent rectal cancer is about 10%. Most patients have serious symptoms after tumor recurrence, which seriously affects the quality of life and prognosis of patients. Although it is more difficult to perform reoperation, it is still the main method to treat local recurrence of rectal cancer. At present, the main methods of reoperation include transabdominal anterior resection, combined pelvic organ resection, combined pelvic organ resection, and sacral resection. Open surgery is the main method. In recent years, with the extensive development of laparoscopic technology, laparoscopic techniques have been tried at home and abroad to perform reoperations for locally recurrent rectal cancer, showing good short-term results. The authors systematically introduce the application of laparoscopic technology in the reoperation of locally recurrent rectal cancer based on relevant research advances at home and abroad, in order to explore its clinical application prospects and promotion value.

Objective To improve the experience of diagnosis and evaluate the clinical efficacy and safety of the surgical management for ureteral fibroepithelial polyp.Methods The clinical date of 29 patients with ureteral polyps admitted in Peking Union Medical College Hospital during 2001 to 2014 were analysed retrospectively.The patients' age was between 1 1 to 84 years and 19 were male.Twenty patients with frank pain and two patients with hematuria were enrolled.Seven patients were found hydronephrosis.Results Twenty-nine cases were treated surgically.Fifteen cases were treated by ureteroscopic laser ablation,10 cases local resection and reanastomosis,1 case of abnormalities duplex kidney and ureter underwent local resection and ureteroplasty,2 case Partial ureterectomy including the polyps and pyeloplasty,1 cases nephroureterectomy because of giant hydronephrosis and nonfunctional kidney.No recurrences were seen during a mean follow-up of 32 months (range 10-56 mos).No ureteral stricture occurs.Conclusions Ureteral fibroepithelial polyps represent a rare pathology.Local resection is the main treatment.Endoscopic management is recommended to minimize morbidity and complications in treatment of ureteral fibroepithelial polyps.Recurrences seem to be rare in these tumors.

Objective To construct and identify the recombinant vector pcDNA3. 1 (-) B/myc-BRMS 1 carrying breast-cancer metastasis suppressor 1 (BRMS 1) which can express in eukaryote cells and which will provide the basis for further researching the mechanisms of metastasis suppression and working on cancer metastasis gene ther-apy. Methods To isolate total RNA from MCF - 7 cells and design a pair of primers, and coding sequence of aRMS 1 cDNA were amplified from human breast cancer cells MCF -7 by reverse transcription-polymerase chain reaction (RT-PCR). Then the product was inserted to the PcDNA3. 1/myc-His (-) B plasmid. The recombined pcDNA3. 1 (-)B/myc-BRMS1 was identified by gene sequence analysis,then recombinants was transfected into HEK-293 cells and was identified by Western blot. Results The recombinant of pcDNA3.1 (-) B/myc-BRMS1 was structurally confirmed by analysis of sequencing. The inserted fragment in the vector was in the right direction and its sequence was structurally confirmed to be consistent with CDS sequence of human BRMSI cDNA that of the published data. GenBank, [AF159141]. The recombinants was transfected into HEK-293 cells ,then the cells expressed protein tagged c-myc identified by Western blot indicated it can express in eukaryote cells. Conclusion cDNA of human BRMS1 can be successfully cloned and inserted into Eukaryote-expression vector. The newly constructed vector may serve as the potential tool to conduct further comprehensive experiments in future on BRMS1 function and on gene therapy.