OBJECTIVE:To observe the effects of Xingnaojing injection on the efficacy and safety of patients with acute cere-bral infarction merge blood stasis syndrome. METHODS:70 patients with acute cerebral infarction merge blood stasis syndrome were randomly divided into observation group(35 cases)and control group(35 cases). Control group received conventional treat-ment according to 2014 edition of Chinese guideline for diagnosis and management of acute ischemic stooke. Patients within throm-bolysis time window were given intravenaus infusion of rt-PA,and those out of thrombolysis time window orally received 0.3 g As-pirin tablet every evening,then 0.1 g after 1 week+40 mg Atorvastatin calcium tablet every evening,and if patients have arfficulty suallowing,gavage administiation was implemented. Observation group additionally received 30 ml Xingnaojing injection,adding in-to 250 ml 0.9% Sodium chloride injection,intravenous infusion,once a day. The treatment course for both groups was 14 d. Clini-cal efficacy,γ-interferon(IFN-γ),interleukin-13(IL-13),interleukin-4(IL-4),high-sensitivity C-reactive protein(hs-CRP)lev-els,the US National Institutes of Health Stroke Scale(NIHSS)rating,improved Barthel index(BI),infarction size before and af-ter treatment and the incidence of adverse reactions in 2 groups were observed. RESULTS:There was no significant difference in the total effective rate and incidence of adverse reactions in 2 groups(P>0.05). Before treatment,there were no significant differ-ences in IFN-γ,IL-13,IL-4,hs-CRP levels,the NIHSS rating,BI and infarction size in 2 groups(P>0.05). After treatment,the IFN-γ,hs-CRP levels,the NIHSS rating and infarction size in 2 groups were significantly lower than before,and observation group was lower than control group,IL-13,IL-4 and BI were significantly higher than before,and observation group was higher than control group,the differences were statistically significant (P<0.05 or P<0.01). CONCLUSIONS:Based on conventional treatment,Xingnaojing injection shows obvious efficacy the efficacy and safety of patients with acute cerebral infarction merge blood stasis syndrome,it can reduce the levels of inflammatory cytokines,improve neurological deficits,daily living skills and in-farct volume,with good safety.

Objective To evaluate the characteristic of left ventricular rotation and twist in healthy adults using ultrasonic velocity vector imaging(VVI),and to discuss its biomechanical mechanism. Methods In 70 healthy adults, the rotational characteristic of left ventricular subendocardium and subepicardium at 3 differently standard shot-axis levels and 18 differently myocardial segments, and the torsional characteristic of left ventricular wall were evaluated via ultrasonic VVI. The maximum rotation angle(Rmax)at different sites of left ventricular wall and the maximum twist angle(Tmax)between left ventricular subendocardium and subepicardium in a completely cardiac cycle were compared respectively. Correlation tests were performed between left ventricular Tmax and the Rmax at different short-axis levels of left ventricular wall.Results ①To be compared with other myocardial segments, Rmax was smallest at antero-septal or anterior wall of the same left ventricular short-axis level(P>0.05).②The mainly rotational directions of the basal leveI and the apical level of left ventricular wall in a completely cardiac cycle were adverse.To be compared with basal or apical level of left ventricular wall,Rmax was smallest at papillary muscle level(P<0.05).and there was no statistical significance in Rmax between basal and apical level.There was positive colinearity between left ventricular Tmax and the Rmax of basal or apical level (P<0.01),but zero correlation was found between the Tmax and the Rmax of papillary muscle level.③Rmax of left ventricular subendocardium was bigger than that of subepicardium(P<0.01).Tmax of left ventricular subendocardium was bigger than that of subepicardium[(11.44±4.82)°vs(7.05±3.90)±,P<0.01].Conclusions Twist of left ventricular wall in healthy adults appeared the torsional characteristic of thin-wall cylinder.Elucidation of the biomechanical mechanism of cardiac twist is promising if basing on the principle of biomechanics.

OBJECTIVE: To assess the effects of navigation and robot technology on percutaneous ultrasound-guided liver tumor ablation. METHODS:The liver model tumors were placed inside excised calf livers, and the needle was guided into the tumor using either conventional ultrasound-guided method or two new methods-3D navigation and a method based on a robotic assistant. Totally 40 experiments were performed, and the distance (all methods) from the tip of the needle to the planning point in the tumors was measured.RESULTS: Based on the measurements, the robotic assistant performed better than both the 3D navigation and the conventional ultrasound-guided method. While, the 3D navigation performed better than the conventional ultrasound-guided method.CONCLUSTON: This study demonstrates navigation combined with robot technique is feasible to improve the precision of deploying ablation needles, future studies are warranted to optimize the system to meet requirements in clinical practice.

Sick sinus syndrome (SSS) is a disease of multiple arrhythmias and symptoms.It has great impact on patients in the quality of life for the symptoms of high incidence.The asynchrony of myocardial electromechanical movement caused by the SSS electrophysiological changes are focused.The ultrasound can not only observe the electrical physiological activity,but also measure the mechanical movement caused by electrophysiological delay.The research progresses of ultrasound in quantitative evaluation of myocardial function in SSS patients was reviewed in this article.

Objective To observe the effects of Xingnaojing injection on cytokine and clinical efficacy of patients with acute cerebral infarction (ACI) and blood stasis syndrome (BSS).Methods A prospective randomized controlled trial was conducted. Seventy hospitalized patients with ACI in the Neurological Department of the First People's Hospital of Jingmen of Hubei Province were enrolled, and they were randomly divided into a combination of traditional Chinese and western medicine group and a control group, 35 cases in each group. Both groups received conventional treatment. The combination therapy group additionally received Xingnaojing intravenous drip 30 mL, once a day on the basis of the control remedy. The therapeutic course in both groups was 14 days. In the two groups before and after treatment, the serum levels of γ-interferon (IFN-γ) and interleukins (IL-2, IL-5 and IL-10) were detected; the neurological function defect scale (NFDS) score, the modified Rankin scale (MRS) score and the daily living activity (ADL) score were observed.Results Compared with the levels before treatment, serum levels of IFN-γ and IL-2 were significantly decreased after treatment [IFN-γ (ng/L): the control group was 32.62±5.34 vs. 45.89±9.28, combination therapy group was 20.45±6.39 vs. 46.02±8.15; IL-2 (ng/L): the control group was 8.62±1.51 vs. 14.23±2.19, combination therapy group was 3.47±0.76 vs. 13.09±1.32], while the levels of IL-5 and IL-10 were obviously increased [IL-5 (ng/L): the control group was 68.39±8.89 vs. 36.80±6.32, combination therapy group was 89.20±9.17 vs. 37.26±6.08; IL-10 (ng/L): the control group was 27.66±5.47 vs. 15.59±3.27, combination therapy group was 38.54±7.66 vs. 16.17±4.29], and the changes in the combination therapy group were more significant [IFN-γ (ng/L):20.45±6.39 vs. 32.62±5.34, IL-2 (ng/L): 3.47±0.76 vs. 8.62±1.51, IL-5 (ng/L): 89.20±9.17 vs. 68.39±8.89, IL-10 (ng/L): 38.54±7.66 vs. 27.66±5.47, allP < 0.05]. Compared with the scores before treatment, NFDS score and MRS score were significantly decreased after treatment (NFDS score: the control group was 12.38±4.26 vs. 27.16±6.35, the combination therapy group was 7.59±2.09 vs. 28.53±7.57; MRS score: the control group was 3.39±1.51 vs. 4.58±1.07, the combination therapy group was 1.39±0.76 vs. 4.65±1.12), but the ADL score was significantly increased after treatment (the control group was 38.17±2.24 vs. 24.25±3.53, the combination therapy group was 48.43±1.32 vs. 23.51±2.17), and the improvement in the combination therapy group was more significant (NFDS score: 7.59±2.09 vs. 12.38±4.26, MRS score: 1.39±0.76 vs. 3.39±1.51, ADL score: 48.43±1.32 vs. 38.17±2.24, allP < 0.05).Conclusion The Xingnaojing injection can significantly regulate the levels of serum cytokines IFN-γ, IL-2, IL-5, IL-10, and improve the recovery of function of damaged nerve and the ADL in patients with ACI.

Objective To study the clinical significance of neuron specific enolase (NSE),carcinoembryonic antigen(CEA) and carcinoma associated antigen(CA125) in diagnosis,evaluation of therapy and monitoring metastasis and recurrence of lung cancer.Methods The levels of NSE,CEA and CA125 were detected in the sera from 30 healthy controls,48 patients with benign diseases,50 patients with non-small cell lung cancer(NSCLC) and 14 patients with small cell lung cancer(SCLC) by using microparticle enzyme linked immunoassay and bioantibodies sandwich one step assay by using streptavidin technique,respectively.Results The levels of NSE and CEA in the sera of patients with NSCLC and SCLC were significantly higher than those of healthy controls and patients with benign diseases (both P0 05).The levels of NSE CEA and CA125 decreased after the treatment and increased greatly with the metastasis and recurrence of the cancer.After the combination of NSE and CEA and CA125,the sensitivity significantly increased,but no difference was found in the specifity.Conclusion Measuring serum levels of NSE,CEA and CA125 are very useful in diagnosis,evaluation of therapy and monitoring of metastasis and recurrence of lung cancer.

Objective To analyze the long term outcomes of antebrachial flap with a pedicle of the posterior interosseous artery Methods Ninety cases were followed up for 1～16 years, the indication and long term outcomes and dorsal aspect of the forearm were analyzed and evaluated retrospectively Results Eighty nine cases survived, except one case, the conteur of forearm and hand were satisfied Conclusion Antebrachial flap with a pedicle of the posterior interosseous artery is an optimal method to repair soft tissue defects of hand.

With the improvement of hospital financial system and promotion of fine management,state-owned assets management will focus on effective methods.This study focuses on the main asset management problems faced by hospitals,such as Iowutilization rate of equipment,Unused inventory,andintangible assets protection.It explores the health economics methods such as demand analysis,market strategyanalysis,cost benefit analysis,cost accounting and budget controi in the public hospital asset management application and practice,in order to explore the public hospital asset management scientific method,and improve the efficiency of asset management.

Objectives To observe the effect of inhibition of transforming growth factor (TGF)?1 on the growth and aggression of colon cancer cells by RNA interference. Methods HCT116 colon cancer cells were transfected with small interfering dsRNA (siRNA), the effect of the inhibition of TGF?1 was tested by semi-quantitative RT-PCR, and changes in cell cycle distribution were analyzed by flow cylometry, the ability of aggression was detected by soft agar colony assay. Results The inhibition of the expression of TGF?1 was significantly detected after the transfection of siRNA against TGF?1, which induced the increase of S phase by 59.0%-82. 5% and decrease in G2 phase, colony assay further demonstrated that siRNA against TGF?1 led to a significant reduction in colony formation as compared with the control group. Conclusions The present data suggested that the expression of TGF?1 was conveniently and rapidly blocked by siRNA transfection, and this may lead to the cell cycle arrest and inhibition of the growth and tumorigenesis of colon cells.

Objective To evaluate clinical safety and efficacy of autologous tissue antigens loaded dendritic cells (DC)in the treatment of elderly patients with gastric cancer.Methods Fresh tumor cells were isolated from surgical specimens in twenty patients with gastric cancer. Single cell suspensions were obtained and induced to apoptosis by heat shock. The apoptotic tumor cells were frozen before use.Peripheral blood mononuclear cells were isolated and cultured with recombinant human granulocyte macrophage-colony stimulating factor (GM-CSF) and interleukin-4 (IL-4).DCs were loaded with apoptotic tumor cells. The immunological and clinical responses were examined after the final vaccination. ResultsVaccination of dendritic cells was well tolerated and no toxicity was observed. The cytokine levels in serum such as IL-2(46 pg/mg vs 89 pg/mg), IL-12(44 pg/mg vs. 86 pg/mg) and IFN-γ(38 pg/mg vs.82 pg/mg) were increased after vaccinations as compared with pretherapy. DTH test were positive in five patients (5/10). The antigen special cells for CD8+/ IFN-γ+ were increased in five patients(5/10). The size of retroperitoneal lymph node of one patient was reduced. The tumor marker CEA level were decreased in five patients, stable in eight patient and increased in two patients.Conclusions Autologous DC vaccines loaded with autologous tumor antigens could improve function of non specific immunity and elicit specific T cells immunologic response and is one of safe and effective treatments for gastric cancer.