Human cytomegalovirus (HCMV) has a higher prevalence in the population, and most normal individuals after primary infection can establish latent infections. Recent reports have suggested that cytomegalovirus infection associated with lipid metabolism, and play an important role in the pathogenesis of many diseases, such as hepatitis, atherosclerosis, isolated syndrome, metabolic syndrome, etc. This article summarized the relationship between cytomegalovirus infection and lipid metabolism, as well as its role in a variety of diseases progression.

Objective To investigate the effect of silodosin,a selective alpha1 a-adrenoceptor antagonist on a rat model of testosterone-induced benign prostatic hyperplasia (BPH) and its mechanisms.Methods The rats were divided into three groups:control,testosterone-induced BPH,and silodosin +BPH groups.BPH was induced by subcutaneous injection of testosterone [20 mg/(kg · d)] for 4 weeks.Meanwhile silodosin + BPH groups rats were administered silodosin 4 weeks [100 μg/(kg · d)].After 4 weeks,all animals were sacrificed to examine the blood biochemical profiles,prostate volume,weight,histopathological changes,and epidermal growth factor receptor (EGFR) and B-cell chronic lymphocytic leukemia (CLL)/lymphoma 2 (BCL-2) protein expressions.Results Each group showed an increase compared to their initial body weight;however,differences in weight change between groups were not significant (P ＞ 0.05).The BPH group displayed lower glucose levels than the control group.The serum levels of glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) were not significantly different among groups (P ＞ 0.05).The group treated with silodosin showed significantly lesser prostate size and weight than the testosterone-induced BPH group [volume:(0.93 ± 0.14) cm3 vs (1.75 ± 0.15)cm3,P ＜0.01;weight:(0.97 ±0.06)g vs (1.30±0.05)g,P ＜0.01].In addition,silodosin decreased the expressions of EGFR and BCL-2 in prostate tissues (P ＜ 0.05).Conclusions These results suggest that silodosin suppress the development of BPH by inhibiting the expressions of EGFR and BCL-2.

Objective To study the different pathogenesis between non-erosive gastroesophageal reflux disease(NERD) and reflux esophagitis(RE).Methods 57 patients with regurgitation or heartburn from 1996 to 2004 were assessed in this study.Patients with esophageal mucosal break by endoscopy were regarded as the RE group,others as the NERD group.The clinical feature and whether complicating with H.pylori infection in both groups were reviewed and all patients underwent esophageal manometry and 24 hours esophageal/gastric pH monitoring.Results No differences of age?sex?smoking?drinking and Hp infection were found between the two groups.The incidence of the atypical reflux symptom (chest pain) in the NERD group was higher than in the RE group.There was pathologic acid reflux in both groups,but the two groups did not differ at reflux degree.Esophageal body peristaltic amplitude of the NERD group was higher than that of the RE group.Average gastric pH of the NERD group in supine was lower compared with the RE group.Conclusion In the NERD patients,the incidence of the atypical reflux symptom is more prevalent.It is the esophageal acid clearance not the reflux degree that plays an important role in the different pathogenesis between NERD and RE.

Objective To clone the 5′ non-coding region (NCR) of human interferon-?-inducible protein 10(IP-10), and to identify the transcriptional activity of IP-10 promoter induced by lipopolysaccharide (LPS) in human umbilical vein endothelial cells (HUVEC). Methods Genomic DNA of lymphocytes was isolated from the human blood. With above DNA as the template, the 5'NCR of human IP-10 was amplified by nest polymerase chain reaction (PCR) method. Then, the IP-10 promoter was cloned into luciferase reporter vector, pGL3. The recombined vector was transfected into HUVEC, and then the activity of the luciferase was determined after the cells were stimulated by LPS. Results Human IP-10 promoter was obtained and the pGL3/IP-10 was successfully constructed. Moreover, the activity of luciferase driven by human IP-10 promoter was observed to obviously increase in the HUVEC stimulated by LPS. Conclusion We successfully cloned human IP-10 promoter, constructed luciferase reporter vector driven by the human IP-10 promoter, and confirmed that high transcriptional activity of human IP-10 promoter was induced by LPS in HUVEC. The results supplied an experimental base for the further study of the transcriptional regulation of human IP-10.

In this study,we have observed the effects of extract from mussel ( Mytilus edulis Linnaeus ) on experimental hyperlipidemia in rats.The results showed that the extract from mussel could reduce the levels of total cholesterol and triglyceride in blood serum of the experimental hyperlipidemic rats.

Fibroblast growth factor 23 (FGF23) is a new endocrine factor, mainly expressed in osteoblasts and osteoblasts. Many studies have found that FGF23 can act on kidney, parathyroid and other tissues after binding with Klotho protein, and participate in bone mineral metabolism. In addition, in recent years, the role of FGF23 in exoskeleton has been gradually discovered, such as FGF23 and thyroid diseases, diabetes and so on. Therefore, understanding the biological characteristics, regulatory mechanism of FGF23 and its relationship with related diseases is of great clinical significance for the diagnosis and treatment of diseases. This article reviews the relationship between FGF23 and metabolic diseases such as phosphorus metabolic disease, parathyroid disease, hyperthyroidism, osteoporosis, diabetes, iron metabolism and so on.

Objective To evaluate advantages and disadvantages of three procedures-percutaneous ethanol injection,small incision unroofing,and laparoscopic unroofing-in the treatment of renal cysts.Methods Clinical data of 106 cases of renal cysts from January 2001 to November 2004,treated either by percutaneous ethanol injection(Percutaneous Group),or small incision unroofing(Small Incision Group),or laparoscopic unroofing (Laparoscopic Group),were reviewed.The operation time,intraoperative blood loss,hospitalization costs,and cure rate were compared among the three procedures.Results The Percutaneous Group presented the least hospitalization costs(3 261.8?779.2 yuan),the lowest cure rate(78.6%,22/28),and the highest recurrence rate(21.4%,6/28).The Small Incision Group had the shortest operation time(44.1?5.7 min),and the Laparoscopic Group had the highest hospitalization costs(9 050.9?1 116.2 yuan).There were significant differences in these parameters among the three groups(P0.05).Conclusions The three procedures,with each having its own advantages,should be selected depending on individual conditions.For patients with a simple renal cyst

We have observed the effects of five alga polysaccharides on rabbit's blood platelets aggregation. We found that the polysaccharides could inhibit aggregation of blood platelets in vitro. The strongest effect was observed in the polysaccharides from the following,in decreasing order of strength: Scytosiphon lomentarius, Codium fragile,Laminaria japonica and Ulvalactuca. The same effect was not observed in polysaccharides from Cladophra. We also found a positive correlation between the effects of anti-aggregation and the ratio of vitriol radicles in these five polysaccharides.

AIM: To study the effect of dexamethasone (DEX) on the adrenomedullin (ADM) and its gene expression in lung tissue of asthmatic rats. METHODS: 30 healthy male SD rats were randomly divided into three groups (10 for each). The asthmatic model was established by ovalbumin inhalation and injection. The mRNA expression of ADM was examined by RT-PCR and the protein expression was detected by immunohistochemical method. The airway wall thickness, the airway smooth muscle (ASM) thickness and pulmonary tissue changes were observed under light microscope. RESULTS: The expression of ADM mRNA and protein in the asthma group A were higher than those in the control group(group C) (P

Objective To distinguish primary CMV infection from reactivation or reinfection. Methods A urea denaturation test was included in the washstep of the standard IgG ELISA in 12 patients of age＜12 whose serum CMV-IgG were positive, avidity indexes of CMV-IgG were measured. Results Among children aged 1～12 years, the varieties of avidity indexes of CMV-IgG in A group(IgM negative and IgG positive) and B group(IgM positive and IgG positive) had the same trend. There was only one case in B group whose avidity index was under 30%, the other were all above 30%, and most were above 50%. But among 40 children aged 1～12 months,the avidity indexes were under 30% in 21 cases,8 cases were 30～50% ,and 11 cases were above 50%. The antibody avidity in 1～6 months children was influenced by their mothers lgG,80% (12/15) of 7 ～12 months children had low-avidity antibodies. Conclusion CMV infection mostly occurred among 1～12 months children. Urea denaturation test was an efficient method to distinguish primary CMV infection from reactivation or reinfection.