Objective To study the clinical characteristics of acute asthma during pregnancy,focusing on management of pregnant women presenting with an acute exacerbation.Methods A prospective observational study was conducted in 30 cases of acute exacerbations during pregnancy.Results The prognosis of 25 patients with mild or moderate asthma was good for both mother and fetus;none had severe complications.Among 5 women who had severe exacerbations,there were 4 pulmonary co-infections,1 with respiratory acidosis,1 with respiratory acidosis plus metabolic allkalosis,and 4 with fetal growth-restriction.Conclusions,This study highlights that pregnancy can worsen asthma control in some women.Effective management and prevention of asthma exacerbations during pregnancy is key to ensuring the safety of the mother and the fetus.

Objective: To determine the distribution of Actinobacillus actinomycetemcomitans (Aa) in subgingival plaques in the patients with chronic periodontitis(CP). Methods: Samples of subgingival plaque were detected for Aa distribution by oligonucleotide probe from 60 sites of CP patients and 10 healthy sites of healthy people.Each sample was obtained from each site of each people. Results: Aa was detected in 19 out of 60 diseased sites(31.67%); Aa wasn't detected in healthy sites.The age(28.68±7.33) of Aa-positive patients was younger than that (44.48±12.48) of Aa-negative patients(P<0.05).Conclusion: Aa is possibly to cause chronic periodontal infection in younger people.

Objective:To evaluate the usefulness of Periocheck in clinical application before and after periodontal curettage.Methods:Periocheck was applied in 65 sites in 65 adult patients with periodontitis within 15 min before and after scaling and root planing to monitor peptidase activity produced by Porphyromones gingivalis (Pg),Treponema denticola(Td) and Bacterides forsythus (Bf) in subgingival plaque. The peptidase activity was determined both spectrophotometrically and by using color standard.Results:The peptidase concentrations (Try U/L) before and after treatment were 0.44±0.23 and 0.34±0.26 (P<0.05),while Periocheck positiveness was found in 65/65 and 43/65 (P<0.01) of the patients,respectively.After treatment clinical parameters such as GI, PD,AL and peptidase concentration decreased (P<0.05) in 22 Periocheck positive patients,while those did not (P>0.05) in 43 negitive.Conclusion:Periocheck might provide a promising chair-side monitoring tool for the evaluation of scaling and root planing.

Objective To explore the abnormal inflammatory cytokine profile of salpingitic infertility by means of cytometric bead assay(CBA). Methods To assay representative inflammatory cytokines of 40 patients of salpingitic infertility(PSI) and 40 healthy control by CBA, and compared 30 samples of serum with peritoneal fluid collected from identical subject. Results Experiment data displayed that in PSI the Th lymphocytes were decreased significantly (t =2. 1236,P <0. 05), Ts lymphocytes increased markedly, and the ratio of Th/Ts markedly decreased ( t = 2. 8392, P < 0. 01 ), compared with those of healthy control.(Pre-)inflammatory eytokines (IL-8, IL-1β, IL-6 and TNF-α) were all significantly higher than those of healthy controls ( t = 4. 9302,5. 5449,3. 4975,5. 6623, respectively, all P < 0. 01 ) ; while anti-inflammatory cytokines ( IL-10, IL-12 ), also increased markedly ( t = 5. 3428,5.9847, respectively, all P < 0. 01 ).Beside IL-10, all other cytokine concentrations presented in serum were higher than that of peritoneal fluid;IL-8(t =2.2113,P <0.05),IL-llS(t =2.9756,P <0.01), IL-6(t =2.3298,P <0.05), TNF-α (t =3. 2895, P < 0. 05 ), IL-12 ( t = 3. 3281, P < 0. 05 ), implying more sensitive of serum samples. Conclusion PSI displayed extensive disorders of the inflammatory cytokine network, CBA can be used to probe the disharmony condition at high through-out and high sensitivity.

A method for the determination of glycyrrhizic acid and chlorogenic acid in Chinese medicinal preparation by RP-HPLC-UV was described. Mobile phase: methanol (0.5%HAc):H2O(0.5%HAc); C18 column and externat standard was used.

Objective:To evaluate the immunogenicity of the recombinant plasmid pcDNA 3.1(+)/kgp_ cd.Methods:BALB/c mice were immunized with recombinant plasmid pcDNA 3.1(+)/kgp_ cd,KGP_ cd protein(control) or vector pcDNA 3.1(+)(control) by quadriceps injection or targeted submandibular gland(TSG) injection. Serum IgG and salivary sIgA levels were assayed by indirect ELISA after immunization. The expression of the protein KGP_ cd in quadriceps and submandibular gland was detected by immunohistochemistry techniques.Results: Serum specific anti-KGP_ cd IgG elicited by pcDNA 3.1(+)/kgp_ cd and KGP_ cd protein was significantly higher in both the quadriceps injection group and the TSG group than that in the pcDNA 3.1(+) group (P

Objective To detect and localize the expression of macrophage colony stimulating factor (M CSF) in human luteinized granulosa cells and investigate the effects of follicle stimulating hormone (FSH) on M CSF production by human luteinized granulosa cells in vitro Methods Human luteinized granulosa cells were isolated from follicular fluid of superovulated infertile patients undergoing intracytoplasmatic sperm injection Some of the luteinized granulosa cells were used for detecting M CSF by immunocytochemical staining (ABC method) Most of them were cultured with HAM′s F 10 medium alone or plus various concentrations of FSH (2, 5, 15, 25 IU/ml) The media were collected after 72 hours and their M CSF contents were measuered by a solid phase enzyme linked immunosorbant assay Results About 80% of the luteinized granulosa cells were positively stained for M CSF antibody The immunoreactive signals of M CSF were specially located in the cytoplasma of the luteinized granulosa cells No immunoreactivity of M CSF was observed in vaginal squamous epithelial cells through contaminations during transvaginal oocyte retrieval The concentration of M CSF in cultured luteinized granulosa cells medium without FSH stimulation was low However, addition of 2, 5, 15, 25 IU/ml concentrations of FSH caused significant dose dependent increases of M CSF production after 72 hours culture by luteinized granulosa cells ( P

Objective To study the effect of nerve growth factor(NGF)in survival of super-length random flap.Methods The experiment employed the animal model of random flap in Wistar's rat back,applied 66.7 μg NGF locally in trial group's flap at the first day after operation with auto-control.The survival proportion,skin temperature and survival rate of random flap were observed,and the blood flow,the blood flow velocity,the blood flow volume change of random flap were measured by the laser and photic-conduct fiber means in differ time.Results The survival proportion(7.12±1.54)cm2 and survival rate(92±5)%of random flap of trial group was obviously higher(P＜0.05)than those in control group[(5.23±0.19)cm2,(69±5)%]after pedicle division.The blood flow,the blood flow velocity,the blood flow volume and skin temperature in the midge and at the end of random flap in the trial group were obviously higher than those in control group 1 day after operation(P＜0.05),and the blood flow velocity in the pedicle of random flap in the trial group was increased markedly(P＜0.05).The blood flow volume and skin temperature in the pedicle of random flap in the trial group were higher than those in control group 3 days after operation(P＜0.05).The survival of randomflapinthetrial groupwere very significantly increased than those in control group 7 days after operation(P＜0.01).Conclusion NGF can increase flap's blood flow,blood flow velocity and blood flow volume,enhancing survival of the big-proportioned and super-length random flap.

Objective To investigate the effect of γ-glutamylcysteine synthetase (γ-GCS),in the kidney fibrosis of MRL/lpr lupus mice.Methods The expression of connective tissue growth factor(CTGF)and γ-GCS in the kidney tissue of MRL/lpr lupus mice and control mice were detected by reverse transcriptase PCR (RT-PCR)and immunohistochemistry.The relationship between CTGF and γ-GCS was analyzed.Results The transcription and expression of CTGF in the kidney tissue of MRL/lpr lupus mice was significantly higher as compared with that of the control mice(CTGF mRNA:1.052±0.004 vs 0.402±0.009,P<0.01;CTGF protein:3.364±0.460 vs 1.206±0.271,P<0.01);whereas the expression of γ-GCS mRNA was decreased in MRL/lpr lupus mice(0.952±0.011 vs 1.145±0.066,P<0.01).Pearson's regression analysis showed that there was a negative correlation between CTGF and γ-GCS(r=-0.902,P<0.01).Conclusion The decreased expression of γ-GCS in the kidney of MRL/lpr lupus mice may compromise the ability of antioxidative stress,and there is a negative correlation between γ-GCS and CTGF.This suggests that the perturbation of γ-GCS may contribute to the progress of kidney fibrosis.

Starch a natural polymer that is characterized by cheap, regenerative, and good biodegradation is generally mixed with low density polyethylene, high density polyethylene, polypropylene, and polystryrene to prepare degradable materials. In order to relieve "white pollution" due to undegradability of plastic materials, starch is mixed with polyvinyl alcohol, polylactic acid, poly-hydroxybutyric acid, polycaprolactone, chitosan, derivatives, and other degradable polymers to prepare fully degradable biomaterials. With gradual exhaustion of petroleum and progressive depravation of environmental quality, starch that is regarded as a reinforcing agent is widely used in rubber industry. Starch is characterized by hydrophilicity, difficulty in processing, and poor compatibility to plastic materials, rubber, and other polymers, so starch is firstly modified and then mixed with polymers to make starch. This study was designed to summarize the application of starch in plastic materials, rubber, and other polymer blends and to investigate the latest research progresses of starch in polymer materials.