ObjectiveTo investigate the prevalence o f erectile dysfuction (ED) in Beijing urban ared,and to study the influence of dem ographic and other risk factors.MethodsUsing systemic epidemiological method, 1247 married men in Beijing urban area were selected by two-stage sample methods.General condition, health status, medications, smok ing and lifestyle was collected by trained interviewers.A self-administered sex ual activity questionnaire (IIEF-5) was used to characterize erectile potency. ResultsThe prevalence rate of ED of married men in Bei jing is 39.1%,and the prevalence rate of ED over 40 years is 54.5%.ED was strong ly correlated with age,especially for men over 60 years.The prevalence rate of c omplete ED in 60～69 years group is nearly 5 times as in 50～59 years group.The ot her important factors correlated with ED include diabetes,vascular disease and s o on.ConclusionsThe prevalence of ED in Beijing urban area and the important risk factors are similar to those reported.Whereas the pr evalence rate of complete ED over 60 years is significantly higher.

Objective To study the polysaccharides from dried rhizome of Ligusticum chuanxiong.Methods DEAE-Cellulose and gel-filtration chromatography were used to isolate and purify the polysaccharides,whose structures were characterized by chemical and spectral methods.Results Four homogeneous polysaccharides,named LCP-1,LCP-2,LCP-3,and LCP-4 were obtained from the water extract of L.chuanxiong.Four structures were investigated to be complicated polysaccharied fractions,with the molecular weights of 3.1?10~4,5.2?10~4,9.0?10~4,and 3.6?10~4,respectively,then estimated by GPC.Conclusion The four polysaccharides are obtained from this plant for the first time.

It was an dideal model of respiratory distress syndyomeinduccd in 59 rabbits by Ⅳ oleic acid injection. Pulmonary hemodynamics, cardiac impedance paramcters were measured, microemboli and leukocytcs were abtained directly from the pulmonary microcirculation by use of the retrograde perfusion method. The results showed that pulmonary hemodynamics were changed significantly, but did not correlate with development of pulmonary edema. It was found that basic impedance (Zo) increased, stroke volume (SV), cardiac output (CO) and cardiac index (CI) all decreased (P

Objective To investigate the expression of tumor necrosis factor-related apoptosis-induce ligand (TRAIL) receptors in bla dder transitional cell cancer. Methods TRAIL receptors m RNA were tested by means of RT-PCR and Northern Blot in bladder transitional ce ll cancer tissue. Results The expression of death recept or 4 (DR4) and death receptor 5 (DR5) have been certificated in bladder transiti onal cell cancer and normal bladder tissue,decoy receptor 1 (DcR1) expressed onl y in normal bladder tissue. Conclusions TRAIL gene may p lay an important role in the apoptosis of bladder transitional cell cancer.

Objective To verify whether the extracellular domain of flt 1 has anti angionesis activity in vivo. Methods A recombinant plasmid pcDNA 3.1/flt 1 n3 was transfected into the human bladder carcinoma EJ cell by lipofectamin, which was constructed by inserting the N terminal first three IgG like domain of VEGF receptor flt 1 (flt 1 n3 ) into eukaryotic expression vector pcDNA 3.1. Results The rflt 1 was functionally expressed in stably transfected EJ and the product secreted in the medium could be specifically bind to rhVEGF165.The result showed that the bladder cancer transfected with rflt 1 had a lower microvessle density than the control. Conclusions It is proved that the expressed rflt 1 n3 can inhibit tumor growth and angiogenesis in nude mice model.

Disease ; genetics ; Humans ; Induced Pluripotent Stem Cells ; metabolism ; pathology ; Models, Biological

Objective To investigate the expressions of CD40 and ICAM-1 in primary hepatocarcinoma and clinical significance and analyze their relationships with carcinogenesis and progression of liver cancer. Methods S-P immunohistochemistry was used to detect the expressions of CD40 and ICAM-1 in 40 cases of hepatocarcinoma and 25 cases of normal liver tissue (distance from cancer tissues≥2 cm).Results The positive expression rates of CD40 and ICAM-1 were 47.5% and 82.5% in liver cancer tissue,which was significantly higher than those in normal liver tissue (P0.05).The positive expression rate of ICAM-1 was associated with lymph node metastasis as well as histological grade(P

Objective To establish HPLC fingerprint of Radix Puerariae from Ankang,Shaanxi Province.Methods Inertisil C18 column(250 mm?4.6 mm,5 ?m) was used,with methanol-acetonitrile-0.25% acetic acid solution as mobile phase in a gradient elution.Flow rate was 1.0 mL/min.The wavelength was 250 nm.Results For the relative retention time and relative area of common peaks,the RSD of ten batches of crude drug samples were below 10%.The similarities accorded with the regulation.Conclusion The analytical method can be used for the quality control of Radix Puerariae and of its sterilized powder for injection.

Objective To investigate the correlations of serum matrix metalloproteinase-2, -9 (MMP-2, MMP-9) contents and tissue expressions in hepatocellular carcinoma with tumor invasion and metastasis. Methods Serum MMP-2, MMP-9 contents were detected in 40 patient with hepatocellular carcinoma and 20 healthy controls by ELISA; the expressions and distributions of MMP-2 and MMP-9 in 40 patients and 10 normal tissues were detected by immunohistochemical method. Results Serum MMP-2, MMP-9 contents were significantly elevated in cancer samples compared with normal serum (P

OBJECTIVE:To investigate the effects and safety of Xingnaojing injection combined with butylphthalide on neuro-logical function,cognitive function,coagulation function and living activity. METHODS:A total of 106 patients with middle cere-bral artery infarction in Wuhan University People's Hospital during Jun. 2014-Dec. 2015 were divided into control group and obser-vation group according to random number table,with 53 cases in each group. Besides routine symptomatic treatment,control group was given Butylphthalide soft capsules 0.2 g,po,tid. Observation group was additionally given Xingnaojing injection 20 mL added into 0.9% Sodium chloride injection 250 mL,ivgtt,qd. Both groups were treated for 14 d. The neurological function score (NI-HSS,NFDS),cognitive function score (MoCA,MMSE),coagulation function indexes (PT,APTT,FIB),BI score were ob-served in 2 groups before and after treatment. The occurrence of secondary remote damage and ADR were recorded. RESULTS:Eight patients withdrew from the study,including 3 patients of control group and 5 of observation group. Before treatment,there was no statistical significance in neurological function scores(NIHSS,NFDS),cognitive function scores(MoCA,MMSE),coag-ulation function indexes or BI scores between 2 groups (P>0.05). After treatment,NIHSS,NFDS scores and FIB of 2 groups were decreased significantly compared to before treatment,and the observation group was significantly lower than the control group,with statistical significance (P<0.05). MoCA,MMSE,BI scores in 2 groups were increased significantly,and PT and APTT in 2 groups were prolonged significantly,and the observation group was significantly higher or longer than the control group, with statistical significance (P<0.05). The incidence of secondary remote damage in observation group (11.3%) was significantly lower than control group (41.5%),with statistical significance (P<0.05). There was no statistical significance in the incidence of ADR between 2 groups(P>0.05). CONCLUSIONS:For middle cerebral artery infarction,the application of Xingnaojing injection combined with butylphthalide can reduce the risk of remote damage,promote the recovery of neurological function and cognitive func-tion,and improve coagulation function and living activity with good safety.