Objective To analyze the efficacy and safety of acupuncture treatment on eczema for providing the objective and reliable evaluation.Methods Cochrane systematic review methods were adopted.The author searched data in the VIP database(1987～2009),Qing Hua Tong Fang database(1997～2009),and China Academic Journals' Full-text Database(CNKJ)(1997～2009)concerned on acupuncture treatment of eczema and evaluated by RCT. Odds ratio(OR) and 95%confidence interval(95%C1)were used as the statistic efficacy for analysis.The quality of the papers was analyzed according to the four quality evaluation criteria in Cochrane Handbook and then meta-analysis(Meta)by RevMan4.2.2 software.Results There were 19 relevant Papers of randomized controlled trials(RCT)and non-randomized controlled trial (CCT)met the included criteria Meta analysis showed that there were statistically significant difference between acupuncture and conventional treatment of eczema.Conclusion Acupuncture treatment can improve the efficiency and reduce the recurrence rate of eczema.However,due to relatively low methodological trial adopted in these included papers,more scientific clinical trial designs were expected for provided high quality papers used for statistical analysis.

Objective To systematically analyze the effectiveness and security of Chinese medicinal ointment treating eczema for the purpose of making an objective and creditable evaluation and guiding clinical usage. Methods Data in the VIP database (1989-2008) and CHKD (1994-2008) were researched and literatures on the treatment of eczema were included. Randomized control trails (RCTs) of therapeutic effects were compared between Chinese medicinal ointment and western medical treatment. Jadad Scale was adopted and RevMan4.2.7 was used to conduct Мeta analysis. Results 11 RCTs were adopted, including 1415 cases.① Heterogeneity Test: It was found through analysis with fixed effect model that Q= 16.57, and P=0.08>0.05,which indicated that the effective between studies are homogenous. ② Total effect size' ORunited was 0.17,with the 95% confidence interval [0.29,0.58]. ③ x2=5.04 (P<0.01), P<0.05, the total ORunited had statistic significance, which indicated that the treatment of eczema with the Chinese Medicinal Ointment was obviously better than that of western medicinal Ointment. Conclusion It is a effective method to treat eczema with Chinese Medicinal Ointment, and there is no serious ADR. However, because of publication bias and RCTs of universal lower quality, the credibility of the conclusion was reduced.

Objective To study the changes of plasma interleukin-13 (IL-13) in patients with acute cerebral stroke and its effects in the cerebral inflammatory reactions. Methods The levels of plasma IL-13 measured in 50 patients with acute cerebral infarction (CI group) and in 30 patients with cerebral hemorrhage (CH group) using enzyme-linked immunoabsordent assay(ELISA) were compared to the control A (with the risk factors of stroke) and control B (normal group).Results As compared with the controls (control A: 28.0?3.2; control B: 26.4?2.7), the levels of plasma IL-13(pg/ml) were increased in three periods in the CI group (37.6?6.2;45.2?10.1;41.3?8.3) and CH group (36.6?4.9;45.3?8.9,38.1?5.6) ( P

Purpose Ovarian dysgerminoma has no specific symptoms or physical signs, which is thus prone to misdiagnosis before treatment. This paper aims to investigate CT and MRI clinical features of ovarian dysgerminoma, so as to provide an accurate basis for its clinical treatment and also improve patients'' life, quality.Materials and MethodsThe CT and MRI data of 13 patients with ovarian dysgerminoma proved by pathology were retrospectively analyzed. Three patients underwent plain and enhanced CT scan, 6 took plain and enhanced MRI scan on T1WI and T2WI with different angles of views, and 4 other patients were performed both CT and MRI scan.Results①Twelve patients had single lesions, and 1 was bilateral multiple ones. The diameter of the lesions ranged from 5.5 cm to 16.5 cm, with mean of (9.5±3.8) cm; in terms of pathological type, 11 belonged to single type and 2 were mixed.②The CT results showed that 6 were solid mass and 1 was cystic. On enhanced CT scan, 6 cases showed heterogeneous light-to-moderate enhancement, 4 had tumors with significantly enhanced blood vessels, and 1 showed heterogeneous significant enhancement at arterial phase; all cases presented continuous enhancement at venous phase.③The MRI manifestations demonstrated 8 were with solid mass, which displayed iso- or slightly hypointense signal on T1WI, and iso- or slightly hyperintense signal on T2WI. The enhanced scan showed that 5 cases had heterogeneous mild enhancement, 3 had moderate enhancement, and 4 were seen with tortuous tumor vessels at arterial phase; all displayed continuous enhancement at venous phase. In the one case of cystic mass, solid component had moderate enhancement at arterial phase and continuous enhancement at venous phase. In another case of multilocular cystic mass, the tumor separate and cyst were significantly enhanced at arterial phase, and were continuous enhanced with hyperintense at venous phase.Conclusion Ovarian dysgerminoma tumors present features such as large lesions with clear boundaries. Tumors of single type are likely to be solid mass with central necrosis; the plain CT scan shows isodensity; the plain MRI scan mainly shows isodensity on T1WI, and iso- or slight hyperintensity on T2WI; the enhanced scan presents slight to moderate enhancement. Tumors of mixed type demonstrate cystic mass; the solid and separate parts are significantly enhanced. The comprehensive analysis of imaging features of ovarian neoplasms could help to make a correct diagnosis before surgery.

ObjectiveTo investigate the efficacy of transurethral resection and ball pouch dilatation for treatment of ureterostenosis.MethodsThe clinical data of 49 cases of ureteral stricture were analyzed retrospective analysis from June 2008 to June 2011 with 20 cases of male patients and 29 cases of female patients.The age was 15 to 56 years,with a mean age of 40 years.Ipsilateral renal function were mild impairment in 4 cases,moderate impairment in 35 cases,and severe damage in 10 cases.There were ureteropelvic junction etenosis in 11 cases,upper ureteral stricture in 13 cases,and lower segment stenosis in 25 cases.The ureteral stricture length was 0.3 to 2.0 cm,with a mean of 1.2 cm.Seventeen patients were treated with transurethral resection and ball pouch dilatation by minimally invasive percutaneous nephrostomy,and 31 cases were completed by ureteroscopy.The ureteral stents were removed by ureteroscope after 3 - 6 months.45 cases were followed up for 12 -43 months,with a mean of 24 months. ResultsForty-eight cases were completed smoothly with 1 case converted to open surgery.The surgical time was 25 to 95 min with a mean of 42 min.The postoperative hospital stay was 2 to 6 d with a mean of 4 d.In the follow-up of 45 cases,B ultrasound and CT scan showed hydronephrosis reduced significantly in 39 patients,IVU showed unobstructed ureter without significant stenosis.And 6 cases showed no significant changes in hydronephrosis. Conc(t)usionThe method of transurethral resection and ball pouch dilatation has good clinical effect,less pain and shorter hospital stays,which provides a new and effective treatment for patients with ureteral stricture.

Objective To evaluate the diagnostic value of surface enhanced laser desorption/ionization time of flight mass spectrom-etry(SELDI-TOF-MS) for Paget disease. Methods The relative contents of serum proteins of 15 healthy people,15 patients with chronic eczema and 20 patients with Paget were detected by Weak cation exchanger protein chip (WCx2) and SELDI-TOF-MS. Results The con-tents of two proteins (3868Da,8876Da) had significant difference in healthy people and Paget disease patients(P<0.01). 291IDa, 3868Da and 5097Da protein peaks had significant difference in chronic eczema and Paget disease(P<0.05). Conclusion It shows great potentiality for early diagnosis and screening the tumor biomarkers of Paget disease with SELDI-TOF-MS.

Objective To evaluate Feridex(superparamagnetic iron oxide,SPIO)enhanced MRI in the diagnosis of hepatic lesions.Methods Feridex-enhanced MRI was performed in 31 patients with CT,or MRI proved or suspected hepatic lesions.T 2WI signal intensity of hepatic parenchyma,lesion and background noise was measured before and after enhancement separately.SNR and CNR of parenchyma and lesion before and after enhancement were calculated.The number of lesions on plain and enhanced scans were observed and alalyzed.Results Post-enhancement SNR of liver significantly decreased (?0.05).Post-enhancement lesion-liver CNR increased significantly (?

Objective To explore the effects of PP242 on the expression of apoptosis protein Bcl?2 in human lens epithelial cells(HLECs). Meth?ods Immortal HLECs SRA01/04 were cultured and treated with different concentrations of PP242. The cell growth was examined by MTT assay at 24 h and 48 h after PP242 treatment. Real?time quantitative polymerase chain reaction(RT?PCR)and Western blot were adopted to detect the mRNA and protein expression of Bcl?2 respectively. Results The cultured HLECs SRA01/04 were collected and treated with different concentra?tions(100,250,500,750,1 000,1 500 nmol/L)of PP242. After treatment for 24 h and 48 h,the inhibitory rate in each well was determined by MTT assay. The inhibition rate was as below,24 h:7.55%,9.43%,16.98%,22.64%,26.42%,30.19%;48 h:11.11%,23.81%,36.51%,42.86%, 49.21%,63.49%. Compared with the control group,the difference was statistically significant(P<0.05). Compared with the control group(0 nmol/L),the expression of Bcl?2 protein was significantly decreased with the increasing concentrations of PP242(P<0.05),while the expression of Bcl?2 mRNA was inhibited by PP242 based on the results of RT?PCR. Compared with the control group(0 nmol/L),the RT?PCR results of Bcl?2 mRNA were 0.723±0.039,0.517±0.028,0.353±0.052,0.167±0.046,respectively(P<0.05). Conclusion PP242 could inhibit cell proliferation of HLECs in vitro with a concentration and time depended manner. Bcl?2 protein is expressed in HLECs,which could be down regulated by PP242 treatment.

Referring to the statistical model for the SNP haplotype phasing which was based on the allele frequencies and advised by Browning SR, we investigated and deduced the phasing method of STR haplotype with linkage disequilibrium inpaternity testing preliminarily. Haplotype phasing of two X-STRs in linkage disequilibrium were illustrated. This method provides an idea for the haplotype phasing of STR markers, which is helpful for interpreting the typing results of STR more scientifically and accurately.

Recombinant protein SMB(PRG4) containing two Somatomedin B domains and a small amount of glycosylation of repetitive sequences of proteoglycan 4 was cloned according to PGR4 gene polymorphism. Mature purification process was established and recombinant protein SMB(PRG4), with high-level expression was purified. By using size-exclusion chromatogaraphy and dynamic light scattering, we found that the recombinant protein self-aggregate to dimeric form. Structure prediction and non-reducing electrophoresis revealed that SMB(PRG4), was a non-covalently bonded dimer.
Glycosylation ; Protein Multimerization ; Proteoglycans ; chemistry ; Recombinant Proteins ; chemistry ; Somatomedins ; chemistry