Objective To investigate the distribution and drug resistance state of Pseudomonas aeruginosa, so as to offer reasonable experimental data for clinical therapy. Methods A statistic analy- sis for the specimen souree, distribution of infected departments and drug resistance state to 441 iso- lates was conducted in a retrospective way. Results Among 441 strains, 357 (80. 95%) were isolated from sputum species. The department with the highest isolating rate was ICU (26.5%), followed by department of respiratory disease (20. 63 %), department of neurosurgery (19.05 %) and department of geriatrics (17. 01%). The most sensitive antibiotic was Meropenem, which total drug resistance rate was 13. 61%; the most insensitive antibiotic was ampicillin-sulbactam, which total drug resistance rate was 91.38%. The drug resistance rate of P. Aeruginosa was increasing year by year. Conclusion Clinical isolates of P. Aeruginosa mostly come from sputum samples and ICU, and they are highly and multidrug resistant to various antibiotics.

[Objective]To evaluate the results of therapy with knee joint synovectomy or articular irrigation in rheumatoid arthritis.[Method]Thirty-six knees in 32 rheumatoid arthritis patients-according to ARA standard were performed with knee joint synoveetomy and articular irrigation.Twenty-two knees in 20 patients(most in grade Ⅰ)were treated with knee joint cavity irrigation operation combined with intra-articular injection of hyaluronic acid,and 14 knees in 12 cases(most in grade Ⅱ)with knee joint synovectomy,All 32 patients received routine anti-rheumatoid drug therapy preoperatively and postoperatively,and were followed-up for 6 months.All the cases were evaluated by the Lysholm scale.[Result]The early symptoms of all knee joints were improved.The excellent and good result rate of articular irrigation-group was 86.4%,and in synovectomy-group was 85.7%.[Conclusion]Satisfactory results can be obtained through articular irrigation combined with intra-articular rejection of hyaluronic acid and concomitant anti-rheumatoid drug therapy in early-stage of rheonmtoid arthritis,but for mid-stage patients,especially with hyperplasia synovium and deslxoyed cartilage,joint synoveetom should he performed early in order to obtain favoring rehabilitation.

0 05) Conclusion Temperature and hemolysis affected the ECP level in different degrees But there was no significant influence of anti coagulation agent on the ECP level It could be concluded from our study that coagulation of the blood was not necessary to the ECP determination

OBJECTIVE: To investigate the effects of Radix notoginseng extracts drug-containing serum on the expressions of apoptosis-regulating proteins including Bax, bcl-2 and p21WAF1 in precancerous gastric cells. METHODS: The N-methyl-N'-nitro-N-nitroso-guanidine (MNNG) transformed eternalized human gastric mucosa epithelium GES-1 cell line (MC cell) was used in vitro as a model of gastric precancerous lesion. The medicated canine serum was prepared by feeding to the adult Beagle dog with Radix notoginseng extracts and obtaining the serum after 2-hour medication. MC cells were cultured with medicated canine serum (medicated serum group) or non-medicated canine serum (normal control group) for 72 hours. Expressions of Bax, bcl-2 and p21WAF1 proteins were detected by immunocytochemical assay and the average optical density of the cells was determined by an image analysis system. RESULTS: Compared with those of the normal control group, Bax and p21WAF1 expressions in medicated serum group were significantly enhanced (P<0.01), while the expression of bcl-2 was significantly reduced (P<0.01). CONCLUSION: Radix notoginseng extracts may inhibit the proliferation and promote the apoptosis of precancerous gastric cells through altering expressions of the bcl-2, Bax and p21WAF1 genes.

Objective　To synthesize and label a tumor positive imaging agent 18F-fluorouracil (FU) and the animal study on the product was also undertaken. Methods　18F-FU was synthesized and labeled. Its biodistribution analysis was done on normal and tumor bearing nude mice. PET imaging was performed on normal and tumor bearing rabbits. Results　HPLC analysis and other quality control test results guaranteed the possibility of animal study and clinical usage of 18F-FU. Biodistribution analysis and PET imaging also demonstrated a high accumulation of the tracer in tumor tissue. Conclusion　18F-FU is a kind of potential tumor positive imaging agents which can be used to assess the effects of chemotherapy.

Objective To study the clinical efficacy and safety of leflunomide combined with irbesartan in the treatment of lupus nephritis . Methods 80 cases of patients with lupus nephritis in Yueqing Hospital Affiliated to Wenzhou Medical University from May 2014 to May 2016 were selected and randomly divided into leflunomide group ( leflunomide combined with irbesartan group ) and cyclophosphamide group ( cyclophosphamide combined with irbesartan group),40 cases in each group.The urine indexes and blood indexes levels,clinical curative effect,adverse reaction of the two groups were statistically analyzed.Results The 24h urine protein,urine beta 2-MG,urine red blood cell count,blood beta beta2-MG,ESR,Cr levels of the leflunomide group were significantly lower (P<0.05),the serum albumin,C3 levels were significantly higher (P<0.05),the total treatment efficiency 97.5%was significantly higher than the cyclophosphamide group 82.5%(P<0.05),the incidence of adverse reactions 5.0%was significantly lower than the cyclophosphamide group 22.5%(P<0.05).Conclusion Leflunomide combined with irbesartan is safe and effective in the treatment of lupus nephritis.

GCC/PKG/VASP,PKA/VASP et.al are associated with the occurrence and development of colorectal cancer.As one of the important signaling molecules of above signal pathways,VASP has constributed to the invasion,metastasis,apoptosis,angiogenesis and other events of colorectal cancer.The role of VASP signaling pathways in the development of CRC and its potential clinlic significance are reviewed in this article.

OBJECTIVE: To study the apoptosis-promoting effect of the serum from Panax notoginseng extracts-fed dog on precancerous gastric cells by means of flow cytometry. METHODS: In the experiment, we adopted eternalized human gastric mucosa epithelium GES-1 cells transformed by N-methyl-N'-nitro-N-nitroso-guanidine (MNNG) (MC cells) as the model of precancerous lesions for study in vitro. We took the serum of a dog before and at two different points of time (2 and 6 hours) after feeding the dog with Panax notoginseng extracts for experiment. The MC cells were cultured in mediums with different concentrations of the medicated serum at 2- or 6-hour point of time for 72 hours. By means of flow cytometry, we examined the apoptosis-promoting effects of the serums on the MC cells. RESULTS: The medicated serums at these 2 points of time had significant effects in promoting MC cell apoptosis. The proportions of apoptotic cells in culture mediums with medicated serums had a significant increase as compared with those in culture mediums with non-medicated serums (serum obtained before administration of extracts to the dog) under the same conditions (P<0.05). The number of MC cells in G(0)/G(1)phase was decreased (P<0.05) and that in G(2)/M phase increased (P<0.05), while no consistent changes were observed in S phase. CONCLUSION: The medicated serums obtained at the two different points of time have significant apoptosis-promoting effects on MC cells. They decrease the number of MC cells in G(0)/G(1) phase and increase the number of MC cells in G(2)/M phase. This is probably responsible for the effects of Panax notoginseng extracts in inhibiting the proliferation of MC cells and promoting its apoptosis.

OBJECTIVE: Through cell cultivation, we studied the inhibiting effects of the serum of the dog fed with Panax notoginseng extracts on precancerous gastric cells, trying to find the best time points or periods when the extracts' function was the strongest after administration of the extracts to the dog. METHODS: The experiments adopted eternalized human gastric mucosa epithelium GES-1 cells and MC cells gained from GES-1 cells transformed by N-methyl-N'-nitro-N-nitroso-guanidine (MNNG) as the model of precancerous lesions for study in vitro. We took the serum of a dog before and at different points of time after feeding the dog with Panax notoginseng extracts for experiment. By means of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay, we examined the inhibiting effects of the serum after culturing the GES-1 and MC cells for 72 hours with different concentration (8%, 4%, 2%) of medicated serum obtained from the dog at different points of time, so as to find that, at which points of time the medicated serum obtained, it would be the most effective. RESULTS: The results showed that the GES-1 and MC cells inhibition rates of medicated serum from the points of 2-hour and 6-hour were the highest, and the culture medium containing 8% of medicated serum from these two points had prominent inhibiting effects on both kinds of cells. The GES-1 cells inhibition rate in culture medium containing 8% of medicated serum from the point of 2-hour was 70.8% (P<0.01) and that of the MC cells was 45.3% (P<0.01). The GES-1 cells inhibition rate in culture medium containing 8% of medicated serum from the point of 6-hour was 88.5%(P<0.01) and that of the MC cells was 42.4% (P<0.01). CONCLUSION: The points of time with the strongest inhibiting effects are 2 hours and 6 hours after being fed with Panax notoginseng extracts. At these two points, the serum is most effective in inhibiting the proliferation of GES-1 and MC cells.

Objective To establish and observe the canine model with esophageal stent implantation for further study of the benign stenosis caused by proliferation.Methods According to orthogonal design,different combinations of two stents and six polytetrafluoroethylene (PTFE) patches were confirmed.Stent was designed as cylinder with mushroom shape on both ends.Beagle dogs (weight 10-12 kg) were adopted and cervical segment of esophagus were dissected.After PTFE patch was encircled around the esophagus,stent was delivered under fluoroscopy.The main body of the stent was located in accordance with the patch.Eating condition and position of the stent were followed on week 1,2,4,6 and 8.Gross specimen was harvested at the end point,and the degree of tissue hyperplasia was evaluated.Each animal model was given a mark according to the eating condition and tissue hyperplasia.Results Eight combinations of stent and patch were provided with orthogonal design.Three models failed for the following reasons:unable to eat in one dog,stent disgorged out in another,and the third died from esophageal necrosis between stent and patch.Four models had obvious tissue hyperplasia on the segment of stent,and weight loss or stent dislocation were observed in each model.One model developed appropriate tissue hyperplasia with normal diet,and stent dislocation was not found during the follow-up.Significant difference was confirmed among 8 models (F =14.7000,P =0.031).Conclusion Animal model with appropriate tissue hyperplasia could be established with following elements:beagle dogs weight from 10 kg to 12 kg; stent 50 mm in length,20 mm in diameter,with top mushroom 10 mm in length,30 mm in diameter,and end mushroom 10 mm in length,25 mm in diameter; PTFE patch 60 mm in length,15 mm in width.