The suppressive effects of cyclosporin A ( CsA ) made in China on immune function were studied in mice. CsA ( 25 and 50 mg/kg ig for 4 d ) caused a significant decrease of spleen plaque forming cell ( PFC ) in mice. At the dose of 25, 50 and 100 mg/kg ig for 4 d, CsA inhibited the production of hemolysin in a dose-dependent manner in mice immunized with SRBC. CsA ( 25, 50 and 100 mg/kg ig for 10d ) also markedly inhibited the delayed cutaneous hypersensitivity ( DCH ) induced by DNCB in mice. CsA 50 mg/kg ig for 14d prolongated survival time of cardiac grafts. The treatment with CsA could not reduce the clearance rate of iv charcoal particles as well as bone marrow cells in mice. There was no significant difference of immu-nosuppressive effect on PFC response between China-made CsA and Sandoz-made CsA

5 cm).The survival rates and the diminished rates of tumors in size were analysed comparatively.Results For small hepatocellular carcinoma,the diminished rates of tumor's size were higher in group PEI than that in group TACE and group CST,and higher in group RF than that in group TACE(P0.05).For large hepatocellular carcinoma,the diminished rates of tumor's size were higher in group TACE and group CST than that in group RF(P

0 05) Conclusion Temperature and hemolysis affected the ECP level in different degrees But there was no significant influence of anti coagulation agent on the ECP level It could be concluded from our study that coagulation of the blood was not necessary to the ECP determination

Objective To investigate the distribution and chemical character of nestin immunoreactive cells in the human basal forebrain. Methods We explored systematically the distribution of nestin immunoreactive cells throughout the human basal forebrain by nestin 331B, 10C2, Rat401 antibody.Furthermore, we investigated the chemical identity of these nestin immunoreactive cells by using double\|labeling immunocytochemistry with NSE, ChAT, p75NGFR, GFAP antibody or using NADPH\|d histochemistry. Results The nestin immunoreactive cells were found in the septum, diagonal band of Broca, innominate substance,amygdala,basal nucleus of Meynert of adult human brain.These nestin immunoreactive cells haven big cell body,2\|3 processes.The nestin immunoreactive cells were labeled by NSE antibody.The large majority of those were single stained,and 28% were double labeled with ChAT positive neurons,15% with NGFR positive neurons,6% with NOS positive neurons.A few nestin immunoreactive cells shared similar morphology with that of astrocyte glia which existed in the spaces between the thin septa pellucida or the midline along the septum.It could not be labeled by GFAP antibody.Conclusion\ A new cluster of nestin immunoreactive neurons that were different from the ChAT,NGFR,NOS positive neurons existed in adult human basal forebrain.\;[

Objective To study the influence of sufentanil on analgesic effect and hemodynamics during recovery period of general anesthesia in elderly patients.Methods Eighty patients with general anesthesia were randomly divided into 4 groups (20 cases in each group).Ten min before surgery ended patients in each group were intravenous injected different dose of sufentanil:group S1 with sufentanil 0.2 μg/kg,group S2 0.4 μg/kg,group S3 0.6 μg/kg,and group C (control group) with saline 5 ml.The time of spontaneous breath restores,the time of summon opening eyes and extubation,the cases occurred cough,nausea,vomiting during recovery period were recorded and the VRS pain scores and Ramsay score 10 min after extubation were also recorded.Mean arterial pressure (MAP) and the heart rate (HR) were recorded before the anesthesia induction(T0),stopped anesthetic (T1),pre-extubation (T2),5 min and 10 min after extubation (T3 or T4)Results As group S1 and S2 compared with group C,there was no significant difference on the time of spontaneous breath restores,the time of summon opening eyes,and the time of extubation and leaving the operating room (P ＞ 0.05).However,there was significant difference on the time of spontaneous breath restores ((7.3 ± 4.1) min vs (10.2 ± 4.7) min),the time of summon opening eyes ((8.1 ± 3.1) min vs (11.2 ± 3.7)min),the time of extubation ((12.3 ± 3.1) min vs (16.3 ± 5.9) min),and the time of leaving the operating room((21.4 ±3.0)min vs (24.2 ±3.5)min) between the group S3 and group C(P＜0.01 or P ＜0.05)Patients occurred cough in group S1,S2 and S3 were less than those in group C (P ＜0.05),but there was no significant difference on the occurrence of nausea and vomiting between group S1-3 and group C (P ＞ 0.05)There was significant difference on VRS pain score and Ramsav score between group S1-3 and group C(P ＜0.01).MAP and HR were increased in all points of time as compared with T0 (P ＜ 0.05 or P ＜ 0.01).And there was significant difference on MAP and HR between group S1-3 and group C (P ＜ 0.05 or P ＜ 0.01).Conclusion Before the end of anesthesia sufentanil 0.2 ～ 0.4 μg/kg intravenous injection could be able to enhance analgesic effect,stabilize hemodynamics and do not affect regain consciousness in elderly patients.

Objective Our study intended to check whether there are any changes of serum concentrations of tumor markers in patients with chronic kidney disease,and to determine the related factors.Methods Atotal of 232 hospitalized patients in Nephrology Department in Zhongshan Hospital of Fudan University from March to June in 2005 were divided into groups respectively according to their levels of Ccr,Upro and Salb.Then Kruskal-Wallis test was applied to confirm the relationships among Ccr,Upro,Salb and serum tumor markers.Furthermore,multielement logistic regression was used to analyze the independent effect of age,Ccr,effusion in serous cavity,the levels of proteinuria and serum albumin on the levels of these markers in CKD patients.Results The serum levels of CEA,CA 199,NSE and SCC in different Ccr groups,the levels of CA 199,CA125,NSE and SCC in different Salb groups,the levels of CA 125,NSE and SCC in different Upro groups,had significant statistical differences.Age was the risk factor of the increased levels of CEA and PSA;effusion in serous cavity was the risk factor of increased levels of CA 125.The decreased level of Ccr was the risk factor of CA 125 and SCC.The elevated Upro was the risk factor of SCC.The decreased Salb was the risk factor of CA 199,CA 125 and NSE.Conclusion When we diagnose some tumors according to their serum levels of tumor markers such as CEA,CA 199,CA 125,NSE,SCC and PSA,we must note that whether the patients are aged or have complications such as large proteinuria,hypoalbuminemia,effusion in serous cavity or decreased kidney function.

Objective:Our previous result showed unravel the functional status of DC in the tissue of gastric cancer (GC) and precancerous lesions, and probed into the possibility of preventing solid carcinoma such as GC.Methods:In the experiment of immunotherapy for the tumor-bearing mice,treated by DC sensitized by mRNA of gastric acncer cells.Results:The indices such as the production rate of ascites of mice(75%),the metastasis rate of tumor(25%),the survival rate of animals (75%) and the average weight of trmor(2.04?0.33 g)showed that the condition of the mice treated by DC was better than that in the control group(P

Objective To study whether inoculating complete or C terminal fragment of A? 42 vaccine could induce normal rats to produce high titer of antibody against A? 42 or not. Methods The COOH\|terminals(36\|42) of beta\|Amyloid 42 were coupled with different carriers to construct subunit vaccine,which been inoculated in normal SD rats sa did the complete beta\|Amyloid 42.Antibodies against A? 42 of the serum and supernatant of homogenated brain were assessed by Western blottting and indirect ELISA.The morphology of brain,liver,spleen and kidney were observed in paraffin section by HE staining. Results After the second inoculation finished,all experiment groups began to develop antibody against A? 42 ,and the titer raising with inoculating times.After four inoculations,The titers of antibody against A? 42 in every experiment group were over than 1∶10?000.Meantime,low titer of antibody against A? 42 could also be assessed in supernatant of homogenated brain.No morphological damages could be detected in the brain,liver,spleen and kidney of the experiment animals.Conclusion\ Normal SD rats can be induced to develop high titer of antibody against A? 42 after inoculating A? 36\|42 vaccine and A? 1\|42 vaccine.There were no morphological damages in several important organs.\;[

Objective To investigate the expressions ofα‐SMA in different esophageal stromal fibroblasts in esophageal car‐cinogenesis .Methods IHC method was uesd to detect the expression of α‐SMA protein in stromal fibroblasts of twenty normal e‐sophageal tissues ,eighty precancerous lesions and fifty esophageal carcinomas respectively .Three kinds of esophageal stromal fibro‐blasts were cultured primarily and cells immunohistochemical staining was carrired out after being purified .Expression of α‐SMA was detected by RT PCR .Results IHC results showed thatα‐SMA expressions in normal ,precancerous and cancerous lesions were of significant differences .RT‐PCR results showed thatα‐SMA expressions were different significantly among three kinds of fibro‐blasts .Conclusion Esophageal stromal fibroblasts were activated with carcinogenensis .AFs was possibly the origion of CAFs .

Objective To explore the influence of different preservation conditions and time period on the testing results of Alzheimer's disease(AD) associated neuronal thread protein(AD7C-NTP) in urine specimen.Methods From Oct.2015 to Jan.2016,urine specimen were collected from 50 AD patients,and divided into three groups,according to the different storage temperature,including room temperature group,4 ℃ group and-20 ℃ group.Preservatives were added into specimen of 4 ℃ preservation group and-20 ℃ preservation group.AD7C-NTP level was detected at different preservation time of all specimen.Results The testing results of AD7C-NTP in specimen of room temperature group and 4 ℃ groups,detected within three days,were not significantly different with initial detection value(P>0.05).After seven days,the testing results in specimen of 4 ℃ group were not significantly different with initial detection value(P>0.05).However,after one day,the testing results in specimen of-20 ℃ group were significantly different with initial detection value(P<0.05).Contrast with 4 ℃ without preservative group,the adding of preservative could not increase the stability of AD7C-NTP.The adding of preservative in specimen of-20 ℃ could obviously increase stability,but the deviation of testing results was beyond acceptable limits.Conclusion 4 ℃ without preservatives could be the optimal storage condition for detection of AD7C-NTP in the urine.