1.Cellular phenotypes and molecular events during adipocyte differentiation
Chinese Journal of Rehabilitation Theory and Practice 2002;8(11):667-669
Adipocyte derives from multipotential stem cell.During the course of differentiation, it covers multipotential stem cell,adipoblast,preadipocyte,immature adipose cell,adipocyte.Each stage has its singular cellular phenotype and specific molecular marker.This article is aimed to review the advance research of this aspect.
4.Study on quality standard for Yanqingsong Granule
Ming YANG ; Zhi LI ; Dazhang WU ; Rongping YANG ; Fei WANG
Chinese Traditional Patent Medicine 1992;0(01):-
AIM: To establish the quality standard for Yanqingsong Granule (Radix P uerariae, Rhizoma Chuanxiong, Radix Paeoniae Alba, Radix Angelicae Dahuricae, et c.). METHODS: Radix Puerariae; Rhizoma Chuanxio ng; Radix Paeoniae Alba; Radix Angelicae Dahuricae. in Yanqingsong Granule were identified by TLC, and the content of puerarin was determined by HPLC. RESULTS: Radix Puerariae; Rhizoma Chuanxio ng; Radix Paeoniae Alba; Radix Angelicae Dahuricae could be identified by TLC. P uerarin showed a good linear relationship at a ran ge of 0.1716~0.8580?g,r=0.9999. The average recovery was 97.49% (RSD= 1.77% and n=6). CONCLUSION: The methods are available with a reproducibility and ca n control the quality of this granule effectively.
5.Study on Gansu Capsule ( sustained release) in pharmacokinetics
Ming YANG ; Rongping YANG ; Qingde HUANG ; Zhi LI ; Xiaobing LIU ;
Chinese Traditional Patent Medicine 1992;0(11):-
Objective: By studying the pharmacokinetics in dogs' vivo of Gansu Capsule (sustained release), evaluate slow release effect in vivo of Gansu Capsule (sustained release). Methods: To take gallic acid as marker component, which is one of effective components, After taking Gansu Capsule (sustained release) and Gansu Capsule (common), to adopt high performance liquid chromatography to determinate the blood concentration of acid pyrogallol in dogs at different time. Results: Both Gansu capsule (sustained release) and Gansu Capsule (common) coincide one compartment model. It suggested that Gansu Capsule (sustained release) had remarkable effects of slow release and could maintain higher blood concentration in vivo longer the slow release effect of Gansu Capsule (sustained release) in vivo that could be evaluated by accumulative releasing percentage in vito. Conclusion: Gansu Capsule (sustained release) had good slow release effect in vivo and vitro.
6.Cloning, Sequence Analysis and Proeukaryotic Expression of Peroxisomal Membrane Protein Allergen Gene from Chaetomium globosum
Zhi-Hua LIU ; Qian YANG ; Li-Ming YANG ;
China Biotechnology 2006;0(04):-
The full length cDNA encoding peroxisomal membrane protein from Chaetomium globosum was cloned using RACE technology and the sequence in cDNA library of C. globosum in GenBank ( Accn: BP099709). The 747bp full length cDNA encoding peroxisomal membrane protein allergen (pero) gene was assembled with 412bp 3'and 508bp 5'RACE products. The open reading frame was 501 bp encoding 166 amino acids. The molecular weight of the protein was 17. 5kDa and its theoretical isoelectric point was 5.75. The pero gene was amplified using specific primers of cDNA 5'and 3'untranslated region, sequence analysis indicated that the gene have 3 exons and 2 introns. ClustalX analysis revealed that amino acids sequence of pero gene from C. globosum and Neurospora crassa shared 83% high similarity. To construct pET28a-pero expressive plasmid, pero gene was inserted into pET28a expressive vector. Escherichia coli BL21 transformed by pET28a-pero plasmid was induced with IPTG. The protein expression was analyzed with SDS-PAGE. A 21kDa pero fusion protein representing the pero gene was expressed in recombinant E. coli BL21. The sequences of cDNA,DNA and deduced amino acid of the pero gene from C. globosum were submitted to GenBank (Accn: AY555771, AY584753,AAS66898).
7.Significance of combined detection of plasma RASSF1A and p16 gene methylation in diagnosis of non-small cell lung cancers.
Gui-zhi LIU ; Yi-ming WU ; Ji-yao YANG
Chinese Journal of Oncology 2007;29(8):613-614
Adenocarcinoma
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diagnosis
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genetics
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Carcinoma, Non-Small-Cell Lung
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diagnosis
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genetics
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Carcinoma, Squamous Cell
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diagnosis
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genetics
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Cyclin-Dependent Kinase Inhibitor p16
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blood
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metabolism
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DNA Methylation
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Genes, p16
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Humans
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Lung Neoplasms
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diagnosis
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genetics
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Tumor Suppressor Proteins
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blood
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metabolism
9.The most promising strategy targeted against cancer stem cells.
Zhi-xiong LIN ; Li-juan YANG ; Shi-ming ZHEN
Chinese Medical Journal 2011;124(24):4303-4303
10.Recent advances of nasal natural killer/T-cell lymphoma.
Yong LIU ; Hai-yu YANG ; Ming-zhi LU
Chinese Journal of Pathology 2005;34(12):809-812