Background Corneal chemical burns,especially hazards of alkali burn become increasingly prominent.Clinically,anti-inflammatory,immuno-suppression,corneal transplantation are the common treating method for corneal alkali burn.But the research of tissue repair under the microenvironment of corneal alkali burn is necessary.Objective The aim of this study was to investigate the therapeutic effect of deproteinised calf serum eye gel on the corneal alkali burn.Methods Alkali burn model of cornea was established on the right eyes by putting the filter paper with 0.5 mol/L NaOH on the center cornea for 1 minute in 24 white rabbits.The model rabbits were divided randomly into 4 groups.Normal saline solution,deproteinised calf serum eye gel,blank matrix gel or recombinant bovine basic fibroblast growth factor(rb-bFGF)eye-gel was topically administered 4 times per day for 14days in the 4 groups,respectively.The inflammatory reaction was examined under the slit lamp and scored based on Ando' s criteria.Corneal fluorescine staining was performed to calculate the corneal ulcer area and scored based on Trousdale' s criteria.Histopathological examination of corneas was performed on the fourtcenth day after experiment.The use of the experiment animals complied with ARVO Statement.Results Corneal edema and opacification were seen in the model eyes with the modeling successful rate 100％.On the seventh day after experiment,the severe ulcer of cornea and hypopyon appeared in the normal saline solution group.Corneal epithelium was intact but the intrarocular structure was invisible in the blank matrix gel group.In th(c) rb-bFCF group,corneal new vessels were seen,however,the corneal ulcer completely regrow in the deproteinised calf serum eye gel group.In 3,5,7,10 and 14days after examination,the corneal inflammatory scores were significantly lower in the deproteinised calf serum eye gel group and rb-bFGF group than those of the normal saline solution(P＜0.01).No significant difference was found in the inflammatory score between the deproteinised calf serum eye gel group and rb-bFGF group (P＞0.05) but was significantly lower than the blank gel matrix group (P ＜ 0.05).With respect to the corneal ulcer,the score was decreased in the deproteinised calf serum eye gel group compared with the normal saline solution group and blank gel matrix group (P ＜ 0.05).Howcver,no significant difference was found in the corneal ulcer score between the deproteinised calf serum eye gel group and rb-bFGF group in various time points (P＞ 0.05).Conclusions Deproteinised calf serum eye gel can promote the healing of corneal ulcer and remit the inflammatory response afler corneal alkali burns with a better effectiveness than rb-bFGF.

Objective To evaluated the expression of vascular endothelial growth factor -C(VEGF-C) in children with neurob-lastomaor willms' tumors. Methods The specimens from 33 cases with neuroblastoma and 30 cases with willms' tumors were detected by immunohistochemical staining method. Results In thirty - three cases of neuroblastoma, stage III VEGF - C positive rate were 37.5% (3/8),stage IV were 32% (8/25);favourable histological(FH) were 45%(9/20),unfavourable favourable histological(UFH) were 30.8%(4/13).Cases with distant meta stasis were 32% (8/25),and cases with lymph node involvement were 66.7%(4/6). In 30 cases with willms' tumors,stage I + II were 26.7% (4/15),while stage III + IV were 40% (6/15), FH were 27.3% (6/22), while UFH were 25% (2/8). Cases with distant metastasis were 33.3% (4/12),and cases with lymph node involvement were 60% (3/5). VEGF-C positive expression rate was significantly higher in tumors from children with lymph node metastasis than those without. Conclusion The study shows that expression of VEGF -C is related to lymph node metastasis.

Abdomen ; surgery ; Abdominal Neoplasms ; drug therapy ; pathology ; surgery ; Adolescent ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Cell Transformation, Neoplastic ; Cisplatin ; administration & dosage ; Doxorubicin ; administration & dosage ; Female ; Humans ; Methotrexate ; administration & dosage ; Neoplasms, Muscle Tissue ; drug therapy ; pathology ; surgery

To investigate formation mechanism of secologanic acid sulfonates in sulfur-fumigated buds of Lonicera japonica, secologanic acid was enriched and purified from the sun-dried buds of L. japonica by various column chromatography on macroporus resin HPD-100, silica gel and ODS. The stimulation experiments of sulfur-fumigation process were carried out using secologanic acid reacted with SO2 in the aqueous solution. The reaction mechanism could be involved in the esterification or addition reaction. The present investigation provides substantial evidences for interpreting formation pathway of secologanic acid sulfonates in sulfur-fumigated buds of L. japonica.
Alkanesulfonates ; chemistry ; Carboxylic Acids ; chemistry ; Chromatography, High Pressure Liquid ; Flowers ; chemistry ; drug effects ; Lonicera ; chemistry ; drug effects ; Models, Chemical ; Molecular Structure ; Sulfur ; chemistry ; pharmacology ; Sulfur Dioxide ; chemistry ; Water ; chemistry

Objective To discuss the frequent etiology of spontaneous sub-cortical hemorrhage and its diag- nosis.Methods The clinical materials of 79 cases of spontaneous sub-cortical hemorrhage were analyzed.Results 56% of the hemorrhage was caused by arterial-venous malformation.48% of the hemorrhage was caused by occult AVM.Conclusion AVM is the most frequent etiology of spontaneous sub-cortical hemorrhage.CTA plays a special role in its diagnosis.

ObjectiveTo analyze the relationship between clinical characteristic pathology and prognosis in infant with intra-abdominal solid tumor.MethodsFifty-two infants(less than 1 year old) with abdominal solid tumor from Apr.1998 to Feb.2007 in Shanghai Children's Medical Center and from Jan.2001 to Feb.2007 in Shanghai Xinhua Hospital were reviewed.The history of these children were reviewed.Features and clinical pathology of these children with their prognosis were analyzed and followed up by telephones and children return visit records from 5 months to 8 years.ResultsLess than 1 month,7 cases;1 month to 1 year old,45 cases.Teratoma 23 cases(44.23%),neuroblastoma 9 cases(17.31%),nephroblastoma 6 cases(11.54%),hepatoblastoma 5 cases(9.62%),epithelioid hemangioendothelioma of the liver 3 cases(5.77%),congenital mesoblastic nephroma 3 cases(5.77%),fusiform cell epithelioid hemangioendothelioma of pancreas 1 case(1.92%),hamartoma of the liver 1 case(1.92%),retroperitoneal small cell malignant tumor 1 case(1.92%).Benign:malignant=1:1.Among the benign tumor,male:female=1:1.Among the malignant tumor,male:female=2.33:1.0.All children were treated with tumor resection,and combined with chemotherapy for those whose tumors were malignant.ConclusionsAmong infant abdominal solid tumors,teratoma and neuroblastoma are much more than other tumors.The cases of benign tumors are almost as much as the malignant tumors.The benign tumors did not have sex differences,and had good prognosis after surgical resection.However,in malignant tumors,the incidence rate of male is obviously higher than female.Completely resection of those malignant tumors with chemotherapy would get little incidence of recrudescence and low case fatality rate.Early diagnosis and early treatment play an important role in prognosis.

Objective Investigate the Asi-antidiarrheal capsule's effect on gastrointestinal horrnones in blood plasma of thyroid hormone-induced diarrheic(Abbreviation:Hyperthyroid Diarrhea.,D)rats.Methods One hundred and twenty SD male rats about 8 weeks old were randomly divided according to their constitution into control group of 10 rats and thyroid hormone-induced diarrheic group of 110 rats.The control group rats welle hvaged with isotonic Na chloride 1 ml/d.Thyroid tablets were made with isotonic Na chloride into 40 g/L susnl. The such solution with 1 ml/d was intragastrically administered to each rat in thyroid hormone-induced diardleic group.After three weeks,blood was sampled from vena caudalis of each rat.FT4 were then detected in blood semm.Fourty-two thyroid hormone-induced diarrheic rats were screened based on FT3 and FT4 level in blood serum, wet stool and body weisht.Fourty thyroid hormone-induced diarrheic rats were stochastically re-divided into 5 groups with 8 in each.The physiological saline with 1 ml/d was given to blank group,1.94 g·-kg-1·d-1 Berberine capsule to positive control group,and 0.63,1.26,2.52 g·kg-1·d-1 to low-dose,moderate-dose and high-dose groups respectivelv. Intragastric administration of each group continued for 7 days.Venous blood was centrifuged before and after administration and underwent radioimmunoassay to observe the effect of Ashi-antidiarrheal capsule on motillty (MTL),gastrin(GAS),somatostatin(SS),vasoactive intestinal polypeptide(VlP)in blood plasma of thyroid homlone- induced diarrheic rats.Results①Weight of thyroid hormone-induced diarrheic rats decreased[(344.0±12.9)g], FT3[(4,58 ±0.70)mol/L]and FT4[(23.44±4.40)mol/L]increased,and weight of wet stool[(17.4±3.2)g] increased.Compared to control group[(386.0±1.8)g,(2.08±0.10)mol/L,(10.18±2.00)mol/L,(9.1±0.6)g], there was a statistical significance(t=6.85,9.80,7.66,7.18,P<0.01).②After treatment,high-dose Ashi-antidiarrheal group[(80.54 ±3.80)ng/L]and positive control group[(90.63 ±9.99)ng/L]blood plasma MTL, compared to pre-therapy[(204.27±17.69),(187.79±13.32)ng/L]was decreased,there wag a statistical significance (t=8.60,4.57,P<0.01)③GAS contentshad respectively decreased comparedtopre-therapy[(192.75±11.80), (193.09±3.81),(190.60±9.31),(196.33±18.13)ng/L]in positive control group[(56.06 ±6.36)ng/L],low- dose group[(90.88±4.18)ng/L],midst-dose group[(75.64±7.09)ng/L]and hish-dose group[(44.32±3.72) ng/L],except for blank group.There Wag a statistical significance(t=15.27,7.62,13.43,13.22,all P<0.01).The intm-group difference of MTL,GAS and VIP level had statistic signifieances before and after the treatment(F= 166.68,1503.53,216.68,P<0.01).Conclusion Ashi-antidiarrheal capsule Can significantly lower the level of MTL and GAS in blood plasma。And raise the level of VIP.

Objective To study the effect of Asi-antidiarrheal capsule on gastrointestinal goblet cell of thyroid hormone-induced diarrhea.Methods Total of 120 SD male rats aged about 8 weeks were randomly divided into 2 groups:control group(10 rats)and thyroid hormone-induced diarrheic group(110 rats).Rats in control group were lavaged with normal saline 1 ml/d.Thyroid tablets were partly desolved into normal saline forming a 40 mg/ml suspension.Rats in thyroid hormone-induced diarrheic group were given the thyroid suspension 1 ml/d to make thyroid hormone-induced diarrheic model.Serum FT3 and FT4 were tested.Fourty thyroid hormone-induced diarrheic rats were screened out according to serum FT3 and FT4 levels,body weight and wet stool.The fourty rats were randomly divided into 5 groups,8 rats in each group:positive control group,berberine group,low-dose,mediandose and high-dose groups.Normal saline of 1 ml/d was admnistered to diarrhea control group,1.94 g·kg-1·d-1 Berberine capsule was given to positive control group,and 0.63,1.26,2.52 g·kg-1·d-1 Asi-antidiarrheal capsule to low-dose,mediandose and high-dose groups,respectively.After sever days treatment,rats are executed.Duodenum,jejunum,ileum and colon were dissected,respectively.Histology observation and cell counting were carried out under light micmscopo on HE coloration.Cell counting unit was defined as:cell/high power field of vision (cells/hpf).Results In jejunum,the number of goblet cells in berberine group,mediandose group and high-dose group[(15.32±2.53),(20.24±1.24),(14.98±1.10)cells/hpf,respectively],were all lower than that of the diarrhea control group[(25.73±4.55)cells/hpf,all P<0.05]with an exception of low-dose group[(23.98±2.28)cells/hpf].The numbers of goblet cells in berberine control group,low-dose group,mediandose group and highdose group[(18.29±1.33),(20.61±2.12),(19.38±2.01),(16.34±1.55)cells/hpf,respectively]were all less than that of the control group[(23.36±3.10)cells/hpf,all P<0.05].The numbers of goblet cells of diarrhea control group and high-dose group were obviously lower than that of the low-dose group(all P<0.05)in jejunum and colon.The numbers of goblet cells of Duodenum and ileum were not significantly different between groups(F=2.81,2.67,all P>0.05).The numbers of goblet cells in the diarrhea control group increased markedly observed under microscope,but decreased following therapeutic treatment.Conclusions The numbers of goblet cells from jejunum and colon in thyroid hormone-induced diarrheic rats are increased significantly.Asi-antidiarrheal capsule can remarkably decrease the number of goblet cells in jejunum and colon,and reduce mucus secretion.

Objective To analyze if there are membrane estrogen receptors(ER)in endometrial carcinoma and if there is some relationship between membrane ER and nuclear ER.Methods The cell membrane and total cell ER?and ER?expressions of high and moderate differentiation endometrial carcinoma cells(Ishikawa and HEC-1A cells)were analyzed.Intermittent immunofluorescence dyeing and fluorescent microscopy were carried out with the ceils treated with polyformaldehyde and Triton X-100. Intermittent immunofluorescence dyeing and flow cytometry were carried out with the live cells and the cells treated with Triton X-100 respectively.Results There were fluorescences on the membrane of the Ishikawa and HEC-1A cells which were treated with polyformaldehyde.When the cells were treated with Triton X- 100,the fluorescences were also seen inside the cells.The fluorescence intensity of ER?and ER?in Ishikawa cell membrane(1.09?0.21,1.27?0.33)was stronger than the control,but there were no significant differences(P＞0.05).When treated with Triton X-100,the total cell fluorescence intensity of ER?and ER?in Ishikawa cell(4.21?0.34,4.69?1.96)was stronger than the membrane(P＜0.05). The ER?and ER?fluorescence intensity of HEC-1A cell membrane(1.58?0.13,1.49?0.04)were stronger than the control(P＜0.05).The fluorescence intensity of ER?and ER?of the HEC-1A cell (2.34?0.33,2.52?0.15)was stronger than the membrane also(P＜0.05).The membrane ER?fluorescence intensity of Ishikawa was lower than HEC-1A(P=0.028).But the total cell ER?fluorescence intensity of Ishikawa was higher than HEC-1A(P=0.002).Conclusions There are membrane ER on endometrial carcinoma cells Ishikawa and HEC-1A.The membrane ER must have some similarity to the nuclear receptor.There is no direct correlation between the quantity of the membrane ER and nuclear ER.

As a part of the project for the Chinese Pharmacopoeia (2015 edition), the quality standard of Sophora flavescens root extract was investigated and established. According to the methods described in the Appendix of Chinese Pharmacopoeia (2010 edition), the water and ash inspections were carried out. The marker components trifolirhizin, sophoraflavanone G, oxymatrine and oxysophocarpine in the samples were identified by qualitative TLC. The determination of oxymatrine, matrine, oxysophocarpine and sophocarpine was conducted by HPLC and the total flavonoids were measured by ultraviolet spectrophotometry, using sophoraflavanone G as reference substance. The results indicated the spots on the plate were clear with good resolution and the contents of oxymatrine, matrine, oxysophocarpine and sophocarpine in the 13 batches of the samples were 3.87% - 11.1%, 0.970% - 4.33%, 1.30% - 2.59% and 0.260% - 1.14%, respectively. The total flavoids in the 13 batches of the samples were 3.88% - 7.93%. In the study, the validated methods were reproducible and the established quality standard was feasible, which could be used for the quality control of S. flavescens root extract and related preparations.
Chromatography, High Pressure Liquid ; Flavonoids ; analysis ; Plant Extracts ; analysis ; Plant Roots ; chemistry ; Sophora ; chemistry