1.Clinical Evaluation of Microwave Tissue Coagulation Treatment of Verruca Vulgaris
Journal of Shanghai Jiaotong University(Medical Science) 2001;21(1):69-70
ObjectiveTo evaluate the effect of microwave tissue coagulation (MTC) to treat verruca vulgaris. MethodsWe observed the lesion healing, post- operation infection and scar forma- tion of verruca vulgaris 182 patients in who received MTC treatment. ResultsThe one - operation cure rate was 87.75 %. The post - operation infection rate was 2.90 %. The healing time varied from 1 to 8 weeks according to the different lesion location. No obvious scar was observed except 2 patients. ConclusionMTC is a convenient, effective and safe method of treatment for verruca vulgaris.
3.Epidemiological Survey of Nodular Gastritis and Duodenal Ulcer before and after Eradicative Treatment of Hpylori Infection
yong-mei, XIAO ; ming, SHEN ; yun, MIAO ; zhi-hong, HU
Journal of Applied Clinical Pediatrics 2004;0(07):-
Objective To investigate the prevalence of nodular gastritis,duodenal ulcer before and after eradicative treatment of helicobactor pylori(Hp) infection in children with gastroscopy.Methods This was a retrospective analysis of 1 275 children,age ranging 1 to 16 years old,collected from our hospital in recent 10 years,detecting rates of before eradicative treatment and after eradicative treatment were analyzed.Gastroscopes were analyzed with regard to a possible association with the infection.Results The detecting rate(11.89%) of duodenal ulcer after eradicative treatment was lower than that(17%) before eradicative treatment(P
4.Curative Effect of Anti-Helicobacter Pylori Treatment on Recurrent Abdominal Pain and Chronic Superficial Gastritis in Children
zhi-hong, HU ; ming, SHEN ; yun, MIAO ; yong-mei, XIAO
Journal of Applied Clinical Pediatrics 2004;0(07):-
0.05).There was significant difference of curative effect before and after treatment of children with RAP(?2=6.74,P
5.Meta analysis for effect of lead on male productive function.
Hai-hong XU ; Zhi-ping CHEN ; Yi SHEN ; Xue WU ; Fan HE
Chinese Journal of Industrial Hygiene and Occupational Diseases 2006;24(10):634-636
Humans
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Lead
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toxicity
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Male
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Reproduction
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drug effects
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Sperm Count
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Sperm Motility
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drug effects
6.Gastric fistulation with transcutaneous endoscopy in a child.
Zhi-hong HU ; Ming SHEN ; Li SUN ; Rong QIAO ; Fu-mei JIA ; Si-yuan YANG
Chinese Journal of Pediatrics 2004;42(3):222-223
7.The clinicopathological significances of LDHA and mutant p53 co-expression in gliomas
Miaoling LAI ; Zhuofang HAO ; Shizhang HUANG ; Xiaoming OUYANG ; Cheng ZHI ; Hong SHEN
The Journal of Practical Medicine 2017;33(14):2275-2280
Objective To investigate the clinicopathological significances of LDHA/mutant p53 co-expres-sion in gliomas. Methods According to the 2016 WHO CNS,archived 68 gliomas were collected and analyzed retrospectively. The co-expression of LDHA/mutant p53 was detected by immunohistochemical staining. Results High expression of LDHA alone was always found in high grade gliomas(48.5%). Mutant p53 high expression was usually observed in glioblastomas (26.5%). There was a close relationship between co-expression of LDHA/mutant p53 in glioblastoma(27.9%,P = 0.005),or gliomas with high histological grading(27.9%,P = 0.002). Conclusions Co-expression of LDHA/mutant p53 in tumor cells might be a specific immunohistochemical pheno-type of gliomas,and may help for distinguishing glioblastoma and other high grade gliomas from low grade gliomas.
8.Effect of integrated Chinese medical treatment (as maintenance therapy) on the survival time of patients with advanced non-small-cell lung cancer: a clinical study.
Ling-Shuang LIU ; Li-Ping SHEN ; Yi JIANG ; Zhi-Fen HAN ; Jian HONG
Chinese Journal of Integrated Traditional and Western Medicine 2014;34(5):526-530
OBJECTIVETo observe clinical effect of integrated Chinese medical (CM) treatment (as maintenance therapy) on the progression-free survival (PFS) and overall survival (OS) in patients with advanced non-small-cell lung cancer (NSCLC) after first-line chemotherapy.
METHODSThe study was a prospective, randomized, controlled clinical trial. Totally 69 non-progressive advanced NSCLC patients treated with first-line chemotherapy were randomly assigned to the test group (34 cases) and the control group (35 cases). Patients in the control group were treated with one Western drug chemotherapy (Gemcitabine or Alimta or docetaxel). Those in the test group were treated with integrated CM treatment (CM decoction, CM Intravenous preparation, and point application). Each cycle consisted of 21 days. Treatment lasted till the disease progressed, or intolerable toxic/adverse reactions occurred, or patients refused to continue the treatment. Patients' life spans were regularly followed-up.
RESULTS(1) The median cycle of maintenance therapy was 2 cycles for two groups with no statistical difference (P =0.274). The median PFS was 12.43 weeks in the test group and 10.00 weeks in the control group, showing statistical difference (P =0.025). The middle survival time (MST) was 18.8 months in the test group and 16.73 months in the control group, showing no statistical difference (P =0.437).
CONCLUSIONCM treatment (as maintenance therapy) showed quail effect to one Western drug chemotherapy in prolonging patients' life span.
Antineoplastic Agents ; therapeutic use ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Carcinoma, Non-Small-Cell Lung ; drug therapy ; Deoxycytidine ; analogs & derivatives ; therapeutic use ; Disease-Free Survival ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Pemetrexed ; therapeutic use ; Prospective Studies ; Taxoids ; therapeutic use
9.Preliminary Study of Bio-transformation of Phytosterol by HPLC-MS
Wei SHEN ; Wei-Hong LIAO ; Zhi-Ming RAO ; Hui-Ying FANG ; Jian ZHUGE ;
Microbiology 2008;0(10):-
The method for analysis and determination the cleavage of soybean sterol, in which the soybean sterol was degraded and the products androst-1,4-diene-,17-dione (ADD) and androst-4-ene-3,17-dion (AD) were developed by Liquid Chromatography-mass Spectrometry. The HPLC conditions adopted were: a All- tima ODS-2 column (250 mm?4.6 mm, 5 ?m), a mobile phase consisted of menthanol-water (70:30), a flow rate of 1.0 mL/min, a room column temperature. and the detective wavelength was 244 nm.The ZMD Micromass electrospray ionization (ESI)-mass spectrometer was employed. In such conditions the corre- sponding HPLC chromatogram and MS spectrum were obtained. The method has a linear ranger of 0.01 mg/mL ~ 0.09 mg/mL, R2 =0.9999, the recoveries of ADD and AD were 102.6% and 105.90%, the RSD of ADD and AD were 3.02%, 3.5% and 3.08%, 3.24%. This method showed high sensitivity, accuracyand easy to perform. It is suitable to analysis the process cleavage of soybean sterol as well as quality control of product.
10.Transfection of embryonic stem cells with green fluorescent protein gene and their differentiation into neural cells
Zhi-yan, SHAN ; Jing-ling, SHEN ; Lei, LEI ; Yan-ning, XU ; Lian-hong, JIN
Chinese Journal of Endemiology 2008;27(4):397-400
Objective To establish embryonic stem cells (ESC) that can express green fluorescent protein (CFP) and differentiate them into neurons. It would provide tagging neurons for clinical transplantation to cure neural system diseases. Methods ESC (R1) was transfeeted with a plasmid containing the GFP by electroporation. A transgeuic cell line was obtained after selection with G418. The ESCs were characterized by AKP staining. Monolayer differentiation method was used to induce neural differentiation derived from GFP-ESC and immunofluorescence method was used to identify Tuj1 positive cells. Results There was no significant difference(X2=3.14,P0.05) in transfect rates between liposome and electroporation (65% vs 79%). The AKP staining of GFP-ESC was positive. GFP-ESC could be differentiated into neural cells. Conclusions These results show that ESC expressing GFP has been estabhshed, which can be differemiated into neurons.