Adult ; Child ; Humans ; Male ; Pain ; etiology ; Pharyngeal Diseases ; etiology ; Rabies ; complications

This paper introuduces two ways of WEIDA LMW-1000 Magnetic Resonance Imaging System's communication with Windows 98 computer. It has a practical value to the improvement of the system's operation interface.
Automatic Data Processing ; Computer Communication Networks ; Magnetic Resonance Imaging ; instrumentation ; Software

0.05)],which less than in control group [(4.614?1.683) IU/cm,(0.119?0.068) IU/cm,(564.2?53.8) ?m Pa

Animals ; Antineoplastic Agents, Phytogenic ; isolation & purification ; pharmacology ; Cholesterol ; biosynthesis ; Drugs, Chinese Herbal ; isolation & purification ; pharmacology ; HIV Protease ; metabolism ; HIV Protease Inhibitors ; pharmacology ; Humans ; Plants, Medicinal ; chemistry ; Platelet Aggregation ; drug effects ; Polysaccharides ; isolation & purification ; pharmacology ; Reishi ; chemistry ; Sterols ; isolation & purification ; pharmacology ; Triterpenes ; isolation & purification ; pharmacology

Objective To assess the proficiency of water monitoring laboratory at rural, county, and provincial levels in Guangdong province, to ensure the province's drinking water monitoring results accurate and reliable. Methods State quality of certified reference materials as water arsenic, fluoride and chloride of 90 copies each were numbered and distributed to 90 monitoring laboratories in the province for testing, The measurement results of the participatory labs were evaluated through normative values and expanded uncertainty, and were compared with those of robust statistics method. Results All participatory labs had timely feedback of their measurement results. The qualified rate was higher when arsenic was tested by hydride generation atomic fluorescence spectrometry and zinc-new silver salt of sulfuric acid spectrophotometric system, while fluoride and chloride by ion chromatography. The average qualified rates of water arsenic, fluoride and chloride of the province's rural drinking water quality monitoring laboratory were 66% (59/90), 72% (65/90) and 72% (65/90), respectively.Seven participatory labs failed the proficiency testing of all three analytes and unqualified rate was 7.8% (7/90)among the ninety participated monitoring labs. The qualified rates of robust statistics method for arsenic fluoride and chloride were greater than those evaluated by the expanded uncertainty, and large deviations with small sample sizes. Conclusions The testing ability of drink-water monitoring labs in Guangdong province has improved.However, by comparison with the requirements of national quality control and testing skills, there is still a gap. It is suggested that internal quality control be included in routine inspeetion to improve laboratory testing technology.

Acute Disease ; Adolescent ; Coronary Angiography ; Coronary Sinus ; abnormalities ; diagnostic imaging ; Coronary Vessel Anomalies ; complications ; diagnostic imaging ; Electrocardiography ; Female ; Humans ; Myocardial Infarction ; diagnostic imaging ; etiology ; Tomography, X-Ray Computed

AIMTo observe the stability of BCG-induced insulin resistance model.

METHODSThe glucose tolerance, serum glucose, FFA, insulin, triglycerides, cholesterol, TNF-alpha and ALT level were measured. The change of GDR was measured by euglycemic clamp in model rats after given i.v. BCG 2, 4 and 8 weeks.

RESULTSAfter 2, 4 and 8 weeks, the GIR and glucose tolerance of the animals deceased significantly. After 2, 4 and 8 weeks, BCG infusion resulted in a pronounced reduction in glucose tolerance and insulin-stimulated glucose disposal rate [GDR = GDR: (29 +/- 6) vs (13 +/- 7) mg.kg-1.min-1 2 weeks; (29 +/- 6) vs (11 +/- 7) mg.kg-1.min-1 4 weeks and (23 +/- 3) vs (16 +/- 3) mg.kg-1.min-1 8 weeks, respectively, P < 0.01]. BCG infusion resulted in a pronounced increase in the weights of the liver [(6.2 +/- 0.9) vs (8.2 +/- 1.3) g, P < 0.05] and spleens [(0.51 +/- 0.11) vs (1.4 +/- 0.4) g, P < 0.01]. The histo-pathological results showed that BCG infusion resulted severe inflammation in the livers and spleens and the ratio of beta/alpha in pancreas increased. The serum levels of triglyceride, FFA and glucose were unchanged, but the level of serum TNF-alpha [543 +/- 60) vs (759 +/- 137) pg.mL-1, P < 0.05] and insulin [(31 +/- 5) vs (36 +/- 5) mu.L-1, P > 0.05] increased.

CONCLUSIONThis novel model of immune insulin resistance is completely and constantly established.

Animals ; Blood Glucose ; metabolism ; Diabetes Mellitus ; metabolism ; Glucose Clamp Technique ; Glucose Tolerance Test ; Injections, Intravenous ; Insulin ; blood ; Insulin Resistance ; immunology ; Male ; Mycobacterium bovis ; Random Allocation ; Rats ; Rats, Wistar ; Spleen ; pathology ; Tumor Necrosis Factor-alpha ; metabolism

OBJECTIVE: To study the distribution of peripheral blood lymphocyte subsets in healthy children aged 0-6 years. METHODS: A total of 826 healthy Han children aged 0-6 years were recruited. According to their age, the children were divided into four groups: newborn, infant, toddler and preschool. Their peripheral blood samples were collected to measure the percentages of lymphocyte subsets by flow cytometry. RESULTS: There were significant differences in the percentages of CD3 T cells, CD3CD4 T cells and CD3CD19 B cells and the CD4/CD8 ratio between boys and girls (P<0.05). The girls had a lower percentage of CD3CD19 B cells, higher percentages of CD3 T cells and CD3CD4 T cells and a higher CD4/CD8 ratio than the boys. The newborn group had the highest percentages of CD3 T cells and CD3CD4 T cells and the highest CD4/CD8 ratio (P<0.05). The percentage of CD3CD4 T cells and the CD4/CD8 ratio gradually decreased with age and the preschool group had the lowest values (P<0.05). The newborn group had the lowest percentages of CD3CD19 B cells and CD3CD16CD56 NK cells (P<0.05). The percentage of CD3CD16CD56 NK cells gradually increased with age and the preschool group had the highest percentage (P<0.05). The percentage of CD3CD19 B cells reached the peak in the toddler period and then decreased with age (P<0.05). The preschool group had the highest percentage of CD3CD8 T cells (P<0.05). The variation trend of distribution of lymphocyte subsets in boys from different age groups was consistent with that in children from different age groups. For girls, the newborn group had the highest percentage of CD3CD4 T cells and CD4/CD8 ratio (P<0.05). CONCLUSIONS The distribution of peripheral blood lymphocyte subsets in healthy children is significantly different across ages and sexes. Therefore, the reference values should be established according to age and sex.
Antigens, CD19 ; B-Lymphocytes ; Child ; Child, Preschool ; Female ; Flow Cytometry ; Humans ; Infant ; Infant, Newborn ; Killer Cells, Natural ; Lymphocyte Count ; Lymphocyte Subsets ; Male

Objective To investigate the relationship among pulsatility index ( PI) , sustained attention function and collateral circulation in patients with severe stenosis or occlusion of internal carotid artery .Methods One hundred and thirty-five patients with severe stenosis or occlusion of internal carotid artery were examined by DSA and were divided into different groups according to the type of collateral circulation .Meanwhile, 30 healthy aged people were selected as a control group .The peak systolic velocity ( Vs) , and diastolic velocity ( Vd) , mean velocity ( Vm) and PI of bilateral middle cerebral artery were obtained by TCD .Sustained attention was evaluated by missed and mistaken scores as well as average reaction time of continuous performance test .The results were compared and analyzed.Results (1)According to the type of collateral circulation , 135 patients with severe stenosis or occlusion of internal carotid artery were divided into groups with patency of anterior communicating artery (AcoA)(31 cases),posterior communicating artery (PcoA)(20 cases), AcoA+PcoA (19 cases), primary collateral circulation (with AcoA, PcoA and AcoA +PcoA) (70 cases), secondary collateral circulation (including ophthalmic artery, leptomeningeal collateral vessel and new blood vessels ) ( 15 cases ) , primary+secondary collateral circulation ( 13 cases) and group without collateral circulation (37 cases).(2)Compared with control group, Vs,Vd,Vm and PI all decreased significantly in every other group ( all P<0.05 ) .Compared with group without collateral circulation , Vd of group with AcoA or primary collateral circulation , Vd and Vm of group with AcoA +PcoA all increased significantly;PI of groups with AcoA , AcoA+PcoA, primary collateral circulation and primary +secondary collateral circulation all decreased (all P<0.05).Compared with group with PcoA, PI of groups with AcoA and AcoA +PcoA both decresed significantly (all P<0.05).Compared with group with secondary collateral circulation , Vs,Vd and Vm of group with primary collateral circulation , Vd and Vm of group with primary +secondary collateral circulation all increased significantly; PI of groups with primary collateral circulation and primary +secondary collateral circulation both decreased (all P<0.05).(3) Compared with control group, the missed, mistaken scores and average reaction time of CPT in every other group increased significantly ( all P<0.05 ) .Compared with group without collateral circulation , the missed, mistaken scores and average reaction time in groups with AcoA , AcoA+PcoA and primary collateral circulation all decreased significantly; the missed and mistaken scores of group with primary+secondary collateral circulation decreased significanly ( all P<0.05) .Compared with group with PcoA , the missed and mistaken scores of group with AcoA decreased significantly while the missed , mistaken scores and average reaction time of group with AcoA +PcoA all decreased significantly ( all P<0.05 ) .Compared with group with AcoA+PcoA, the missed, mistaken scores and average reaction time in group with AcoA increased sinificantly ( all P<0.05).Compared with group with secondary collateral circulation , the missed, mistaken scores and average reaction time in group with primary collateral circulation all decreased significantly ;the missed and mistaken scores in primary+secondary collateral circulation both decreased significantly ( all P<0.05 ) .( 4 ) PI positively related to average reaction time in groups with AcoA , primary and secondary collateral circulation ( r=0.441, r=0.364, r=0.552; all P<0.05 ) .PI positively related to missed scores in group with PcoA and group without collateral circulation (r=0.668, r=0.397;all P<0.05).PI also positively related to mistaken scores in the above groups (r=0.509,r=0.480;all P<0.05).Conclusion High PI on the affected side of patients with severe stenosis or occlusion of internal carotid artery may reflect impairment of sustained attention function .

Objective In order to detect gastric fluid pH value,Gastrin-17 (G-17),pepsinogen Ⅰ (PGⅠ),pepsinogen Ⅱ (PGⅡ) and figure out gastric secretory function in patients of functional constipation (FC).Methods 51 healthy individuals were chosen as control group and 42 patients with FC were chosen as FC group,of which serum gastric fluid pH value,Hp ratio,G-17,PGⅠ and PGⅡ were detected and analyzed.Results Compared with control group,gastric fluid pH value were marked reduced (t=2.180,P=0.032),and G-17,PGⅠ level in blood were marked increased in FC group (G-17:t=2.703,P=0.008;PGⅠ:t=7.388,P＜0.001).Hp ratio and blood level of PGⅡ in two groups showed no significant difference (Hp ratio:x2=0.031,P=0.861;PGⅡ:t=1.666,P=0.100).Conclusion Increased gastric secretory function level were found in patients of functional constipation.It gave a hint that stimulated gastroenteritic axis existed in patients of functional constipation.