? AlM: To investigate the therapeutic effects of Pranoprofen on tear dynamic abnormity induced by conjunctivochalasis ( CCh) .
?METHODS: Seventy cases ( 70 eyes ) whose the main symptoms was epiphora induced by CCh were divided into II, Ⅲ and Ⅳ in 3 groups according to Zhang’s grading standards, meanwhile, all the patients were treated with the eye drops of Pranoprofen for 2wk. After that, the curative effect of each group was analyzed before and after treatments according to the symptoms, dye disappearance test, bitter taste test and tear break-up time.
?RESULTS: After 2wk treatment of Pranoprofen, the symptom of patients at grade I and II improved obviously grade Ⅲ improved more, grade Ⅳ did little. The positive rate of dye disappearance was the highest in grade II, the second in grade Ⅲ, and the third in gradeⅣ. There was significant difference among the 3 groups (P<0. 05). The time of bitter taste test was shortened (P<0. 05) and the tear break-up time was prolonged (P<0. 05) in grade II after treatment, however, the time of those in gradeⅢandⅣhad not changed than before ( P>0. 05).
?CONCLUSlON: Pranoprofen has good effects on tear dynamic abnormity induced by grade II of cch.

The purpose of this study is to investigate the activity and mechanism of a new anti-tumor agent T03. MTT and colony formation assay were performed to determine anti-proliferation activity of T03 in vitro. Antitumor activity was observed by Renca xenograft model in vivo. The effect of T03 on cell cycle and apoptosis were measured by FCM analysis. Western blotting was performed to investigate the expression level of proteins in HepG2 cell lines treated with T03. T03 had anti-tumor activity by inhibiting tumor cell growth and colony formation in vitro, especially on hepatocellular carcinoma cells (HCC). At the concentration of 10 micromol x L(-1), T03 induced cell apoptosis and cell cycle arrest in HCC. Moreover, it proved that T03 reduced the tumor weight with the rate of 42.30% without any obviously side effect in Renca xenograft model. At the concentration of 2.0 micromol x L(-1), T03 was able to reduce the level of p-c-Raf (Ser259), and thus blocked Raf/MEK/ERK and AKT signaling in HepG2 cell lines. The result suggested that T03 has the potential to inhibit cell proliferation and induce cell apoptosis both in vitro and in vivo, particularly active against HCC, indicating T03 and its analogues may serve as a new anti-cancer drug against hepatocellular carcinoma.
Animals ; Antineoplastic Agents ; pharmacology ; Apoptosis ; drug effects ; Carcinoma, Hepatocellular ; pathology ; Cell Cycle Checkpoints ; drug effects ; Cell Proliferation ; drug effects ; Hep G2 Cells ; drug effects ; Humans ; Liver Neoplasms ; pathology ; Signal Transduction ; drug effects ; Xenograft Model Antitumor Assays

Objective To explore the relationship between intracellular calcium levels ([Ca2+]1) and reactive oxygen species (ROS) in sodium fluoride (NaF)-induced injury in human neuroblastoma SH-SY5Y cells. Methods The levels of [Ca2+]1 and ROS were measured in different exposed times(0,3,6,12,18,24 h) respectively after SH-SY5Y cells were exposed to 40 mg/L NaF in vitro, and the optimal expose time was selected. Furthermore, the changes of [Ca2+]1, ROS and LDH levels in 40 mg/L NaF-treated groups incubated with 38.23 mg/L BAPTA-AM or 380.40 mg/L ethylene glycol-bis-(beta-aminoethyl ether)-N, N, N', N'-tetraacetic acid (EGTA) or 16.32 mg/L N-acetyl-L-cysteine(NAC) also observed at the optimal expose time(12 h), respectively. Results At 3,6,12,18 and 24 h, [Ca2+]1 level(5620.0±226.3,4775.5±85.6,3312.3±87.5, 3047.0±75.0,2717.0±66.5) was significantly increased, and so was the ROS level(4449.53±324.61,7463.07±117.43,20 227.33±178.04,8817.56±200.13, 7975.61±92.90) except at 3 h, compared with 0 h(2115.0±24.0,4098.01±21.22, all P<0.05). The levels of [Ca2+]1 and ROS reached the peak at 3 h and 12 h, respectively. [Ca2+]1 and LDH levels in NaF-treated group [3279.5±94.0, (1057.50±64.35)U/L], NaF+NAC treated group[ 3583.0±350.7, (561.02±85.50)U/L], NaF+EGTA treated groups[3701.5±157.7, (1074.50±86.97)U/L], and BAPTA-AM treated group[2766.5±38.9, (521.43±40.80)U/L] had increased, compared with the control[2022.5±118.1, (186.97±8.73)U/L], the difference being statistically significant (P<0.05). ROS levels in NaF-treated group (19 003.04±332.34), and NaF+EGTA treated group(19 170.12±95.46) was higher than that in the controls(4060.98±145.66), the difference being statistically significant (P<0.05). NaF and NAC had antagonistic effect on ROS and LDH levels (F=976.11,43.54,P<0.05). And NaF and BAPTA-AM had antagonistic effect on [Ca2+]1, ROS and LDH levels (F=15.65,1515.53,115.00, P<0.05). Conclusions NaF-related calcium is released from the site of intracellular calcium storage, which induces ROS production, both of them caused cytotoxicity and the increase of LDH level in human neuroblastoma SH-SY5Y cells.

BACKGROUNDThe morbidity and mortality of prostate cancer have been increasing rapidly in recent China. There were few studies investigating prostate-specific antigen (PSA) values ranges in the healthy Chinese population. We performed this study to determine the distribution of serum PSA in a large healthy Chinese population.

METHODSFrom January 2001 to May 2008, 11 150 healthy Chinese men aged 30 - 79 years came to our hospital for routine health check-up. All subjects without a previous diagnosis of prostate cancer, a history of prostate surgery, or urogenital tract infection were proposed to undergo systematic serum PSA measurement and digital rectal examination (DRE). Men with normal DRE and PSA ≤ 4.0 ng/ml and those PSA > 4.0 ng/ml or abnormal DRE but without adverse findings on prostate biopsy were included (n = 9358). Age and serum PSA concentration were recorded and correlated through Logistic regression analysis.

RESULTSThe 95th percentile serum PSA concentration was 1.89 ng/ml for men aged 30 to 39 years, 2.19 ng/ml for men aged 40 to 49 years, 2.88 ng/ml for men aged 50 to 59 years, 4.42 ng/ml for men aged 60 to 69 years, and 6.52 ng/ml for men aged 70 to 79 years. The serum PSA concentration correlated with age (P < 0.0001) with an annual increase of 0.97% for men in 40 years, 1.58% for men in 50 years, 3.04% for men in 60 years, and 3.99% for men in 70 years.

CONCLUSIONSThe serum PSA level correlates directly with age in Chinese men older than 40 years, not in Chinese men younger than 40 years old. Chinese men have lower PSA level compared with white men above 60 years of age, not in those under 60 years of age.

Adult ; Age Factors ; Aged ; Asian Continental Ancestry Group ; Humans ; Male ; Middle Aged ; Prostate-Specific Antigen ; blood ; Prostatic Neoplasms ; blood ; epidemiology

OBJECTIVETo observe the therapeutic effect of abdominal acupuncture on nerve root cervical spondylosis.

METHODSThree hundred cases were randomly assigned to a treatment group and a traction control group. The treatment group were treated with abdominal acupuncture at an acupoint prescription, i.e. Zhongwan (CV 12), Guanyuan (CV 4) and others were selected. The control group were treated with suspended traction by a cervical vertebral extraction belt at pillow-jaw sitting position. The short-term and long-term therapeutic effects were observed at the end of therapeutic course and 90 days after the end of treatment, respectively.

RESULTSThe total short-term and the long-term therapeutic effects were 97.3% and 92.7% in the treatment group, and 82.0% and 82.0% in the control group, respectively, with significant differences between the two groups (P < 0.01).

CONCLUSIONAbdominal acupuncture has better short-term and long-term therapeutic effects with short therapeutic course.

Acupuncture Points ; Acupuncture Therapy ; Humans ; Medicine, Chinese Traditional ; Spinal Nerve Roots ; Spondylosis ; therapy

OBJECTIVETo investigate the polymorphism of angiotensin II type 1 receptor (AT(1R)) gene A1166C and environmental factors on hypertension of Kazakh people.

METHODSThrough the random program of SPSS 13.0, 220 cases were randomly selected from the confirmed hypertension patients, and 220 cases with normal blood pressure were selected as control group. All cases were investigated through the questionnaire and the related indexes were measured. By polymerase chain reaction (PCR) method, the genotypes were determined.

RESULTS(1) In hypertension group, the genotype frequency of AA and AC were 78.6% (173/220) and 21.4% (47/220), respectively, compared with control groups' 81.4% (179/220) and 18.6% (41/220), no significant difference was identified between these two groups (chi(2) = 0.537, P > 0.05). In hypertension group, the frequency of A and C allele were 89.0% and 11.0%, respectively, compared with 90.3% and 9.7% in control group. There was no significant difference between these two groups (chi(2) = 0.37, P > 0.05). (2) AC genotype might interact with excessive salt consumption (hypertension group, 31/220, 15.5%; control group 10/220, 4.5%, OR: 4.67, 95%CI: 2.15 - 10.15), overweight (hypertension group, 19/220, 8.6%; control group, 9/220, 4.1%, OR: 6.96, 95%CI: 2.33 - 20.76) and drinking large volume of salty milk (hypertension group, 20/220, 9.1%; control group, 10/220, 4.5%, OR: 2.67, 95%CI: 1.11 - 6.42) which will raise hypertension hazard of AC genotype.

CONCLUSIONThere is no relationship between the A1166C gene polymorphism of AT(1R) gene and hypertension of Kazakh people. AC genotypes might also interact with food consumption habit and behavior factors and increase the individual risk of hypertension.

Adult ; Alleles ; Case-Control Studies ; China ; epidemiology ; Feeding Behavior ; Female ; Gene Frequency ; Genotype ; Humans ; Hypertension ; epidemiology ; ethnology ; genetics ; Male ; Middle Aged ; Polymorphism, Genetic ; Receptor, Angiotensin, Type 1 ; genetics ; Smoking

OBJECTIVETo develop a method for evaluating the feasibility of prenatal screening using local median value and determining the cut-off value.

METHODSWith receiver operating characteristic curve (ROC) analysis, results of second trimester prenatal screening calculated by a local median value in a new model and the built-in median value in 2T software were compared. The cut-off value was set by serial analysis of true and false positive rates and other relevant data.

RESULTSThe ROC curve has accurately estimated the difference in the screening efficacy between a local median value and that embedded in the 2T model, and established a reasonable cut-off value for the laboratory based on false positive rate and detection rate.

CONCLUSIONThe method of ROC curve can be used to evaluate the performance of local median value in prenatal screening and to test the rationality of cut-off value established in the laboratory. As the result, a better cut-off value may be derived.

China ; epidemiology ; Down Syndrome ; diagnosis ; epidemiology ; genetics ; Female ; Humans ; Male ; Pregnancy ; Pregnancy Trimester, Second ; genetics ; Prenatal Diagnosis ; instrumentation ; methods ; standards ; ROC Curve ; Software

Sensory integration treatment (SIT) corrects sensory dysfunction by controlling and regulating sensory input in the environment. SIT was currently used more and more widely, especially for children with behavioral and developmental disabilities. Based on the published researches for children in the past five years, SIT may work well for the population with something such as sensory processing and attention deficit hyperactivity disorder. Other specific results were different in the studies, and difficult to conclude. The research should be improved in the dificiencies as small sample size, confusing dosage and terrible fidelity. Intervention planning for individuals requires the integration of the information with specific knowledge of the patient's needs, expert consensus and professional judgment.

Objective To explore the effects of Astragalus polysaccharides on liver injury induced by cadmium chloride. Methods The cadmium chloride solution was administrated i.v. to develope rat model of liver injury. Rats were randomized divided into three groups;control group, model group, Astragalus polysaccharides intervention group. After the 5 weeks, the rats were sacrificed and the livers were weighted, alanine aminotrans ferase(ALT), aspartate aminotransferase (AST) and lactate dehydrogenase(LDH), the contents of cadmium(Cd) was checked by plasma mass spectrometerthe(ICP-MS), the contents of interleukin-2(IL-2) and transforming growth factor-β1(TGF-β1) was measured by ELISA, the the protein expression of Bcl-2 and Bax was observed by immunohisto-chemistry staining method. Results The liver weight was increased in model group, the level of ALT, AST and LDH were also ascended in model group, the contents of Cd, TGF-β1 and the protein expression of Bax all increased in mode group(P＜0.05 or P＜0.01). The content of IL-2 and the protein expression of Bcl-2 were decreased in mode group (P＜0.05 or P＜0.01). Compared with the model group, the liver index was decreased in Astragalus polysaccharides intervention group, the levels of ALT, AST and LDH were also reduced in Astragalus poly-saccharides intervention group, the contents of Cd, TGF-β1 and the protein expression of Bax were all dropped in Astragalus polysaccharides intervention group(P＜0.05 or P＜0.01).The content of IL-2 and the protein expression of Bcl-2 increased in Astragalus polysaccharides intervention group(P＜0.05 or P＜0.01). Conclusions Astragalus polysaccharides may reduce the oxidative stress injury of rats liver cells indued by cadmium chloride, and its mechanism may be explained by regulation of Bcl- 2/Bax protein expression.

Objective To explore the role of Astragalus polysaccharidesin renal tissue under cadmium treatment in rats.Methods The SPFWistar rats were randomized divided into three groups:normal group,model group,experimental group.The normal group was intraperitoneallyinjected with 0.9％ saline solution,while the model group and theexperimental group were intraperitoneally injected daily with cadmiumchloride (CdC12) in a single dose of 1.5 mg· kg-1 body weight.At thesame time,the experimental group was given Astragalus polysaccharide20 mg · kg-1 once a day for five consecutive weeks.The model group andthe experimental group were given 0.9％ saline solution once a day for five consecutive weeks.After the last injection,all the rats were sacrificed by femoral artery sampling.The content of cadmium (Cd) was measured by atomic absorption spectrophotometry.The levels of superoxide dismutase (SOD),malondialdehyde (MDA),lactate dehydrogenase(LDH),glautathione peroxidase(GSH-Px) were checked by colorimetric analysis.The contents of interleukin -2(IL-2),transforming growth factor-3 (TGF-β) were determined by enzyme linked immunosorbent assay.The protein expressions of Bcl-2 and Bax were analyzed by immunohistochemical method.Results IL-2 level in normal group,model group,experimental group were (117.38 ± 10.89),(71.82 ± 14.56),(102.46 ±13.82)pg·mL-1,respectively;TGF-β1level in above groups were (4.88 ±0.38),(9.77 ±0.21),(6.35 ±0.41)pg·mL-1,respectively;GSH-Px level in above groups were (240.48 ± 18.24),(159.26 ± 21.06)(191.50 ± 23.82) U · g-1,respectively;LDH level in above groups were (1125.25 ± 37.91),(2089.46 ± 35.87)(1159.61 ± 28.86) U · g-1,respectively;Cd level in above groups were (0.02 ± 0.01),(19.68 ± 1.85)(12.99±2.11)mg · kg-1,respectively;SOD level in above groups were (71.55 ± 3.56),(39.30 ± 3.36)(67.25±2.68) U · mg-1,respectively;MDA level in above groups were (3.96 ± 0.79),(7.47 ± 0.78)(5.61 ±0.99) nmol · mg-1,respectively;Bcl-2 gray level in above groups were 37.54±3.23,68.43 ±5.2138.25 ± 6.17,respectively;Bax gray level in above groups were 57.87 ± 8.93,27.54 ± 5.45,48.23 ± 3.54.respectively.Compared with the normal group,all factors in the model group were significantly (all P ＜ 0.01).Compared with the model group,all factors in the experimental group were significandy (P ＜ 0.05 or P ＜0.01).Conclusion Astragalus polysaccharides pretreatment has a certain protective effect against cadmium-induced renal damage in rats and the protection is achieved by the regulation of Bcl-2/Bax protein expression.