1.Pay attention to clinical study of drugs.
Chinese Journal of Hematology 2008;29(7):433-434
2.Effect of Temperature on Lycopene Prodmction by Recombinant E.coli and Control Strategy
Microbiology 1992;0(05):-
Batch fermentation of lycopene by recombinant E. c ol i ZYL-2 at various temperatures ranging between 23℃~37℃ were studied in 7L fermentor. At 33℃, cell specific growth rate in earlier stage of culture was higher, and the time obtained maximum cell dry weight was shorter at other tempe rature. while after 9 h, lycopene specific production rate was higher at 28℃. Based on these results, a two-stage temperature control strategy was developed in which 33℃ was used for fermentation for the first 9 h and the temperaturew as switched to 28℃ after 9h. Using this temperature-shift strategy, the maxim al lycopene content and productivity reached 605.25?g L-1 and 28.82?g L-1h-1. The lycopene fermentation level obtained by the strategy was higher than those in single temperature-control experiments.
4.Association between unique nucleotide polymorphism of 2350G→A in angiotensin converting enzyme and myocardial infarction in Han nationality
Min PAN ; Wen-Ping JIANG ; Zhi-Hua LIU ; Xiang-Jun YANG ; Zhi-Chu CUI ; Dong-Lei ZHANG ; Jian-Hua ZHU ;
Chinese Journal of Emergency Medicine 2006;0(05):-
0.05).Conclusions SNP of 2350G→A in ACE gene is associated with MI,AA genotype is probably a genetic marker of MI in Han nationality.
5.Identification of phenylpropanoids in ciwujia injection by HPLC-MS.
Jing HUANG ; Qing SHAO ; Yan-Hua XIANG ; Zhi-Wei GE ; Xiao-Hui FAN
China Journal of Chinese Materia Medica 2014;39(13):2513-2520
There are reports about the chemical compounds of Ciwujia herbs, but with no study report about the chemical material basis of Ciwujia injection (CWJI). In this study, LC-MS(n) and LC-Q-TOF-MS techniques were adopted for a qualitative analysis on phenylpropanoids in CWJI. The Ultmate XB-C18 column (4.6 mm x 250 mm, 5 microm) was adopted and eluted with the mobile phase of 0.5% formic acid-water and acetonitrile, with the flow rate at 0.8 mL x min(-1) and the column temperature at 20 degrees C. Based on the data of high-resolution and multi-stage MS, control products and literatures, altogether 54 phenylpropanoids were identified in Ciwujia Injection, including 34 phenylpropanoids, 16 ligans and 4 coumarins. Among them, 28 were reported for the first time in Ciwujia, and 14 compound structures were identified in comparison with the control products. The method established in this study could be used to simply and rapidly identify phenylpropanoids in CWJI. The findings provide scientific data for defining the chemical material basis of CWJI.
Chromatography, High Pressure Liquid
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methods
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Drugs, Chinese Herbal
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chemistry
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isolation & purification
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Eleutherococcus
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chemistry
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Mass Spectrometry
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methods
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Molecular Structure
6.Collision tumor of small lymphocytic lymphoma and histiocytic sarcoma: report of a case.
Lan-xiang GAO ; Guang LIU ; Guang-zhi YANG ; Hua-ye DING
Chinese Journal of Pathology 2009;38(11):775-775
Antigens, CD
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metabolism
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Antigens, CD20
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metabolism
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Antigens, Differentiation, Myelomonocytic
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metabolism
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Antineoplastic Combined Chemotherapy Protocols
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therapeutic use
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Axilla
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Histiocytic Sarcoma
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drug therapy
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metabolism
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pathology
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Humans
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Immunohistochemistry
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Leukemia, Lymphocytic, Chronic, B-Cell
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drug therapy
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metabolism
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pathology
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Male
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Middle Aged
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Receptors, IgE
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metabolism
7.Therapeutic effects of hydroxychloroquine combined with butyli flufenamatum ointment and other drugs for the treatment of polymorphous light eruption: a comparison study
Sitong LI ; Xiang NONG ; Tongyun LIU ; Hua GU ; Zhi YANG ; Li HE
Chinese Journal of Dermatology 2015;48(6):395-399
Objective To evaluate the efficacy and safety of hydroxychloroquine combined with butyli flufenamatum ointment and other drugs for the treatment of polymorphous light eruption (PLE).Methods A total of 48 patients with PLE were randomly and equally classified into group 1 and group 2.Both groups took hydroxychloroquine 200 mg twice a day and loratadine 10 mg per day for the initial 4 weeks,then took hydroxychloroquine 100 mg twice a day alone for another 4 weeks.Group 1 also topically applied butyli flufenamatum ointment twice a day during the 8 weeks,while group 2 applied mometasone furoate cream twice a day for the first 2 weeks followed by butyli flufenamatum ointment twice a day for another 6 weeks.Each treatment cycle lasted 2 weeks,and both groups received 4 cycles of treatment.Patients were evaluated for the response rate at the end of each cycle,and for the total symptom score and erythema score before and after the 8-week treatment.Statistical analysis was carried out using t test,chi-square test,Fisher's exact test and repeated-measures analysis of variance with the SPSS17.0 software.Results On day 14,28,42 and 56,the total score improved in 0,3,12 and 19 patients in group 1 respectively,and in 1,4,12 and 20 patients in group 2 respectively;the erythema score improved in 1,5,13 and 18 patients in group 1 respectively,and in 0,5,11 and 17 patients in group 2 respectively.No significant difference was observed between the two groups in response rates at any of the above four time points (P > 0.05).Both the total score and erythema score significantly decreased after the 8-week treatment in both groups compared with the pretreatment scores (both P < 0.05).No serious adverse reaction was observed in either of the two groups.Conclusions Hydroxychloroquine combined with loratadine and butyli flufenamatum ointment shows high efficacy and safety for the treatment of PLE.Topical butyli flufenamatum ointment is highly effective for the treatment of PLE,especially for PLE cases mainly presenting with erythema.
8.Research progress on the risk factors and pathogenesis of climatic droplet keratopathy
International Eye Science 2020;20(10):1744-1747
Climatic droplet keratopathy(CDK), an acquired corneal degenerative disease, is characterized by oil droplet deposits and banded opacity in the pre-corneal elastic layer and stroma layer, which can severely affect the visual acuity of patients. Recently, many studies have indicated that various factors caused the occurrence and development of CDK. However, the pathogenesis and specific pathogenesis of the disease remain unclear. So this article aims to summarize four aspects of the CDK, including the epidemiological characteristics, the morphology of corneal lesions, the composition of corneal deposits and the ocular surface micro-environment, and then provide a reference for ophthalmologists to acknowledge and explore CDK deeply.
9.Analysis of the reconstruction of binocular visual function in 76 cases of pediatric ocular trauma
Ling, WANG ; Gui-Xiang, LIU ; Zhi-Juan, XU ; Ai-Hua, ZHANG
International Eye Science 2011;11(6):954-957
AIM: To evaluate the effect of binocular visual function training in pediatric ocular trauma. METHODS: There were 76 patients (76 eyes) that were hospitalized with a primary diagnosis of ocular injury at the Affiliated Hospital of Medical College, Qingdao University between January 2006 and December 2009. Binocular visual function training was given after primary wound repair. Far stereopsis function were checked using AIT-1000 synoptophore fusion, and near stereopsis function was checked using Titmus stereogram.Binocular visual function was compared before and after training. RESULTS: Before binocular visual function training,26 eyes(34%)had no binocular vision, after training there were only 16 eyes(21%) without binocular vision. Before undertaking binocular visual function training with fusion, only 27 eyes (36%) had binocular vision, after the training there were 48 eyes (63%) with binocular vision. Before undertaking binocular visual function training with far stereopsis ,there were 23 eyes (30%) with binocular vision, after the training there were 29 eyes (38%). Before binocular visual function training with near stereopsis, there were 14 eyes (18%) with binocular vision, after the training there were 33 eyes (43%) with binocular vision. There was a significant difference in the number of patients with binocular vision before and after binocular visual function training. CONCLUSION:The training is useful for the reconstruction of binocular visual function in pediatric ocular trauma.
10.Comparison of 99Tcm-MIBI myocardial perfusion imaging and delayed enhancement MRI for patients with idiopathic dilated cardiomyopathy
Zhi-xin, JIANG ; Wei, FANG ; Chao-wu, YAN ; Shi-hua, ZHAO ; Jian, ZHANG ; Zuo-xiang, HE
Chinese Journal of Nuclear Medicine 2011;31(4):245-249
Objective To compare 99Tcm-MIBI MPI with delayed enhancement MRI (DE-MRI) in patients with idiopathic dilated cardiomyopathy (IDCM). Methods Forty patients with IDCM were included. They underwent both rest 99Tcm-MIBI myocardial perfusion imaging and DE-MRI within 7 days. 99Tcm-MIBI MPI was performed to identify diffuse or segmental abnormal perfusion patterns including reduced or defect perfusion segments. DE-MRI images were divided into 4 categories: no delayed enhancement, septal, subendocardial and transmural delayed enhancement, x2 test was used for data analysis. Results Diffuse and segmental perfusion abnormality on 99Tcm-MIBI MPI were found in 19 (47.5%) and 21 (52.5%)patients respectively, while DE-MRI enhancement was simultaneously found in 5 patients of the former (5/19, 26.3%) and 18 (18/21, 85.7%) of the latter (x2 =14.401, P<0. 001). For those (n=18) with both segmental perfusion abnormality and DE-MRI enhancement, the number of segments of the 4 DE-MRI respectively. A significant difference was found in the DE-MRI enhancement categories between normal and defect perfusion segments (x2 = 29. 183, P <0.001 ) and between reduced and defect perfusion segments as well (x2 =25. 110, P<0. 001). Conclusions Both diffuse and segmental perfusion abnormalities on 99Tcm-MIBI MPI can be found in patients with IDCM. DE-MRI enhancement is more frequently found in patients with segmental perfusion abnormality.