3.Effect of Local Delivery of Paclitaxel on Anastomotic Wound Healing of the Common Bile Duct in Guinea Pig
Xiaoqing ZHANG ; Gang YIN ; Zhi XU
Chinese Journal of Minimally Invasive Surgery 2005;0(08):-
Objective To investigate the effect of local delivery of paclitaxel on bile duct scar formation and biliary stricture in guinea pig.Methods Models of end-to-end anastomosis of the common bile duct were established in 42 guinea pigs.The animals were randomly divided into paclitaxel and control groups.In the paclitaxel group,the drug solution(1000 ?mol/L,0.05 ml) was smeared onto the anastomotic wound before closing the abdomen.The animals were killed in 3 days,2 weeks,and 1 month after the operation(7 in each group at each time point).The sepcimens of the common bile duct were obtained for histological examination.Transmission electron microscopy was employed to observe the ultrastructure of paclitaxel-induced apoptotic cells.Results Histological examination showed inflammatory reaction and hyperplasia of the bile duct wall.The bile duct wall of the paclitaxel group was significantly thiner than that of the control group in 3 days and 1 month after the operation [3 days:(574.41?24.53)?m vs(802.98?24.42) ?m,t=-6.604,P=0.000;1 month:(1383.36?36.64) ?m vs(1518.56?34.89)?m,t=-2.672,P=0.020].Paclitaxel-induced cell apoptosis and injured cell organs were found by transmission electron microscopy.Conclusion Single local delivery of paclitaxel can inhibit biliary stricture in one month.
4.Improve clinical scientific research with a solid base in clinical work
Changsen FENG ; Zhi ZHANG ; Gang WU
Chinese Journal of Medical Science Research Management 2010;23(3):216-封3
It is a common phenomenon that medical research is out of line with clinical work at present. In fact, there is intrinsic interrelation between clinical work and research. Thinking and exploration at clinics are part of the research, and any research based on chnical work not only has great value, but also receives strong support from the state. However, to translate clinical research into clinical practice is difficult, which requires evidence-based methodology, a knowledgable team with persistence andtechnologies, et al.
6.Posterior wall reconstruction of external auditory canal and tympanoplasty after modified radical mastoidectomy.
Zhi-Gang ZHANG ; Xiang LIU ; Sui-Jun CHEN
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2009;44(7):597-598
Adult
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Ear Canal
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Female
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Hearing Loss, Conductive
;
surgery
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Humans
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Male
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Mastoid
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surgery
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Middle Aged
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Postoperative Period
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Tympanoplasty
7. Clinical observation on effets of high frequency oscillating ventilation on patients with early acute respiratory distress syndrome
Medical Journal of Chinese People's Liberation Army 2013;38(1):58-61
Objective To investigate the effect of high frequency oscillating ventilation (HFOV) on patients with early acute respiratory distress syndrome (ARDS) and its mechanism. Methods Through self controlled study, in 25 patients who met criteria of ARDS, their PaO2/FiO2, Paw, arterial blood pH, PaCO2, Cst, cadiac index (CI), heart rate (HR), central venous pressure (CVP), percentage pulmonary shunt (QS/QT), oxygen index (OI) and incidence rate of ventilator associated lung injury (VALI), ventilator associated pneumonia (VAP) were recorded 1, 3, 5 days before and after HFOV. Results Five days later, X ray examination showed that all of the patients got better. Seven days later, 3 patients died because of original diseases, but their symptoms of ARDS improved. Three days after HFOV treatment, the value of PaO2/FiO2 (172.5±69.8) and Cst (63.4±10.5) increased compared with baseline (95.5±29.5, 31.5±4.5, P<0.05, P<0.01), while OI(12.8±8.1) and QS/QT (0.28±0.11) decreased compared with baseline (25.4±13.5, 0.46±0.12, P<0.05). There were not significant difference in Paw, PaCO2, arterial blood pH, CVP, HR, CI and related parameters at different time point after treatment (P>0.05). Conclusion HFOV can improve oxygenation index and pulmonary complications of patients with early ARDS.
8.Cancer stem cells:current status
Zhi-Gang SUN ; Sheng-Dong HUANG ; Bao-Ren ZHANG ;
Academic Journal of Second Military Medical University 1981;0(04):-
Recently,study on cencer stem cells has been a focus of study.Cancer stem cell is a small population of cencer cells possessing the properties of stem cells:self-renewal,differentiation and proliferation.To date,the existence of cancer stem cells has been proven in acute and chronic myeloid leukemia,breast cancer,brain tumors,liver cancer and colon cancer,etc..In this article we reviews the current progress on cancer stem cells,including the defination,existing evidence,research methods, and challenges in clinical application.
9.Breeding of Bacillus tkuringiensis (Bt) with High Insecticidal Activity to Spodoptera exigua
Ji-Xing WU ; Zai-Er CHEN ; Zhi-Gang ZHANG ;
Microbiology 1992;0(03):-
By the breeding model of physics mutagenests (60Co,UV light) and re-strong in the body of insects, We obtained a Bt strain Bt99985 with high insecticidal activity. The shake flask and 40t tank fermentation tests showed that, this strain showed special activity against Spodoptera exjgua, Comparing with original Bt strain: 93005, CK strain: HD-1-580 and GC-91, the value of potency increased respectively by 429%, 655% and 114% in shake flask test. Comparing with GC-91, the value of potency increased by 180% in 40t tank fermentation test.
10.Effects of total flavonoids of Herba Epimedii on the metabolism of typeⅠcollagen and the expression of cathepsin K in the bone of ovariectomized rats
Zhi-Gang ZHU ; Li-Ge SONG ; Xiu-Zhen ZHANG ;
Chinese Journal of Endocrinology and Metabolism 1986;0(03):-
Objective To investigate the effects of total flavonoids of Herba Epimedii(HEF)on the metabolism of typeⅠcollagen and the expression of cathepsin K in the bone of ovariectomized(OVX)rats. Methods Fifty-four female SD rats were allocated into 6 groups;OVX group,sham operation group,OVX rats followed by three doses of HEF(40,80 and 160 mg?kg~(-1)?d~(-1))and nilestriol(0.1 mg?kg~(-1)?d~(-1))for 12 weeks respectively.Bone mineral density(BMD)of whole body was determined by dual-energy X-ray absoptiometry.The level of cross-linked N-telopeptide of typeⅠcollagen(NTx)in the urine were determined by ELISA.The amounts of typeⅠcollagen protein and cathepsin K protein in bone tissue were detected by immunohistochemical method and Western blotting.Results Compared with OVX group,the total BMD values in the HEF treated groups were increased(all P<0.05),and the expression levels of typeⅠcollagen in three HEF treated groups rose significantly in a dose-dependent manner after 12 week,and simultaneously,both the expression of cathepsin K in bone and the level of NTx/Cr were reduced markedly(P<0.05),being most significant(P<0.01)in the group treated with the highest dose of HEF(160 mg?kg~(-1)?d~(-1)).Conclusion HEF seems to be able to elevate BMD and improve bone quality of rats via promoting synthesis and inhibiting proteolysis and absorption of typeⅠcollagen in the bone.