Objective To screen immune molecules expressed on peripheral blood mononuclear cells (PBMCs) which arc related to the aggravation of chronc hepatitis B (CHB). Methods Real-time polymerase chain reaction (PCR) was employed to detect the relative expression of 39 immune molecules at mRNA level and then compare the differences between groups (control group, mild CHB group, moderate CHB group and severe CHB group). The investigated immune molecules included leukocyte differentiation antigens, chemokine receptors, apoptosis-related ligands and receptors,adhesion molecules and Toll like receptors. Scheffe model was used to compare the differences among all groups and Spearman rank correlation was used for correlation analysis. Results Among the 39 immune molecules, the expressions of tumor necrosis factor related apoptosis inducing ligand (TRAIL), TRAIL receptor (TRAIL-R) 2, CD64, CD30, CD27, CD28, L-selectin (CD62L),intercellular adhesion molecule-1 (ICAM-1), chemokine (C-X-C motif) receptor (CXCR) 2 were significantly increased in severe CHB group compared with those in control group ( 1.96, 2.13, 1.33,1.16, 1.57, 2.14, 2.03, 2. 10 and 2.09, respectively; all P＜0.05). The expressions of TRAIL,TRAIL-R2, CD64, CD30, CD27 were highly correlated with the expression of interferon gamma (IFN-γ) (r=0. 816, 0. 572, 0. 462, 0. 697 and 0. 793, respectively; all P＜0.01). The expressions of TRAIL-R2, CD64, CD30, CD62L, ICAM-1 were highly correlated with the expression of tumor necrosis factor alpha (TNF-α) (r=0. 494, 0. 588, 0. 568, 0. 968 and 0. 976, respectively; all P＜0.01). Conclusion The abnormal expression of TRAIL,TRAIL R2,CD64,CD30,CD27,CD28,CD62L,ICAM-1 and CXCR2 may play an important role in the pathogenesis and aggravatoin of hepatitis B.

Anti-Bacterial Agents ; therapeutic use ; Endophthalmitis ; complications ; drug therapy ; Humans ; Infant, Newborn ; Meningoencephalitis ; complications ; drug therapy ; Suppuration

Child ; Glucocorticoids ; therapeutic use ; Hand, Foot and Mouth Disease ; drug therapy ; Humans

Objective To explore the expression of adrenomedulin(ADM) and its receptors mRNA in newborn rats with hypoxic-ischemic brain damage(HIBD).Methods Eighty 7-day-old Wistar neonatal rats were randomly assigned 10 groups,to the sham- operation group(the left common carotid artery without ligated and no hpoxia environment),HIBD different time points groups(0,2,4,6,8,10,12,24,48 hours).The expression of ADM mRNA was measured in cortex at different time points by the reverse-transcription polymerase chain reaction(RT-PCR).Results Expressions of ADM and its receptors mRNA in neonatal rats with HIBD increased,and after HIBD 2-4 hours,had a significant difference compared with the sharn-operation group,and reached the highest after HIBD 8-10 hours(P

OBJECTIVE:To establish a method of determination of midecamycin A1 in midecamycin tablets METHODS:This method used C18 column as stationary phase,0 1mol/L ammonium formate solution-acetonitrile(80∶55) as mobile phase and 232nm as detection wavelength RESULTS:There was a good linear correlation in the range of 0 5～8mg/ml,r=0 9 995,S=5 989C-24 65,RSD=1 4%(n=5) The mean recovery was 99 35% and RSD=1 2%(n=6) CONCLUSION:The method is simple and easy to do and may be adopted for quality control of this drug

Child, Preschool ; Female ; Humans ; Vasoactive Intestinal Peptide ; secretion ; Vipoma

Objective To investigate the effects of abnormal psychological factors on patients with different subtypes of gastroesophageal reflux disease(GERD)and on their natural history.Methods Patients with GERD symptoms were underwent 24 hour esophageal pH monitoring and endoscopy.They were enquired about frequency and severity of reflex symptoms and other informations,and filled in the Symptom Checklist 90(SCL-90).Twelve months later,they were enquired again about their symptoms and medication.Results One hundred and fifteen patients were followed up for(18.0?6.1)months (ranged from 12 to 37 months).The values of psychosomatic parameters including somatic,anxious, depression,compulsory and psychiatric scores in patients with GERD and RE were higer than normal values(both P

Objective To observe the effects of asthmatic children treated by inhaled salmeterol xinafoate and fluticasone propi-onate powder. Methods One hundred and fourty cases of moderate and severe asthmatic children were treated in non- acute period aged from 4 to 14 years by inhaling salmeterol xinafoate and fluticasone propionate powder, compared with control group treated by flu-ticasone propionate in 106 cases, and the pulmonary function was monitored simultaneously. Results The total effective rate of the treatment group and control group were 99. 3 % , 99. 1 % , respectively.The pulmonary function indexes such as the first one second expiration volume(FEV1), flow velocity of 50 % expiration vital capacity(FEF50%), peak expiration velocity(PEF1) after being treated 4 months was improved significantly compared with those before treatment.The difference between them was statistically significant (P

Humans ; Infant, Newborn ; Sturge-Weber Syndrome