Objective To clarify the frequency, the routes of transmission from mother to infant, the correlation factors and distribution of genotypes of transfusion transmitted virus (TTV) infection. Methods Nested polymerase chain reaction (n PCR) was performed to detect TTV DNA and genotypes in serums and breast milks from 160 cases of pregnant women. Results TTV DNA in serum and breast milk was detected in 64 (40 0%) and 60 (37 5%) of 160 cases of pregnant women respectively. The positive rates of TTV DNA from HBV markers (+) and normal groups were 50 0%, 43 1% and 13 6%, 22 7% respectively in serums and breast milk. There were significant difference between the two groups ( P

Objective: To evaluate the impact of loss of a spouse on self-rated health among middle-aged and elderly populations in China, so as to provide insights into health promotion among middle-aged and elderly populations with loss of a spouse. Methods: Based the 2018 China Health and Retirement Longitudinal Study (CHARLS) database, married and separated individuals or individuals with loss of a spouse at ages of 45 years and older and with monthly household consumption of no less than 100 yuan were enrolled. Subjects' age, gender, educational levels, marital status, monthly household consumption, number of chronic diseases and self-rated health were collected. Subjects were classified according to loss of a spouse, and the impact of loss of a spouse on self-rated health was evaluated among middle-aged and elderly populations using propensity score matching (PSM). In addition, heterogeneity test was performed to evaluate the gender- and educational level-stratified impacts. Results: Totally 3 272 valid subjects were enrolled, including 1 618 men (49.45%) and 1 654 women (50.55%), and there were 512 subjects with loss of a spouse (15.65%). There were 656 subjects with very good self-rated health (20.05%), 622 with good health (19.01%), 1 534 with fair health (46.88%), 376 with poor health (11.49%) and 84 with very poor health (2.57%). There were 3 152 couples with successful nearest neighbor matching, and there was no significant difference between the subjects with and without loss of a spouse after adjustment for variables (P>0.05), while the overall fitting degree of the model was good following matching. PSM showed that the self-rated health became poor following loss of a spouse among middle-aged and elderly populations, with average treatment effect (ATT) of 0.055 to 0.061, and a remarkably negative effect of loss of a spouse on self-rated health was seen in women, with ATT of 0.192 to 0.223, while no remarkable effect was seen in men. Loss of a spouse showed a remarkably negative effect on self-rated health among individuals receiving junior and senior high educations, with ATT of 0.048 to 0.057, but presented no remarkable effect on self-rated health among individuals receiving primary educations. Conclusions Loss of a spouse presents a negative effect on self-rated health among middle-aged and elderly populations, and remarkably negative effects are seen among women and individuals receiving high educational levels.

Alprostadil ; administration & dosage ; adverse effects ; therapeutic use ; Hearing Loss, Sudden ; drug therapy ; Humans ; Injections, Intravenous ; Lower Extremity ; Male ; Middle Aged ; Pain ; etiology

Aged ; Cardiovascular Diseases ; diagnosis ; etiology ; Cerebrovascular Disorders ; diagnosis ; etiology ; Diagnostic Errors ; Female ; Humans ; Laryngitis ; complications ; diagnosis ; Middle Aged

Objective To evaluate a preliminary outcome of volar surgical treatment of dorsally displaced fractures of distal radius with open reduction and internal fixation with T shape locking compression plate.Methods From January 2009 to January 2012,32 cases with dorsally unstable distal radius fractures were treated with open reduction and internal fixation with T shape locking compression plate.Results All patients were followed; the average follow-up period was 12 (8-18) months.The patients all healed well.According to Dienst scoring system,the results were excellent in 23 cases,good in 7 cases,fair in 2 cases,the excellent and good rate was 93.75％(30/32).Conclusions The volar fixation of T shape locking compression plate for dorsally displaced fractures of distal radius has advantage of stable and reliable,high healing rate,low rate of complications and early motion.It is especially suitable for osteoporosis patients.

Amnion ; surgery ; transplantation ; Animals ; Humans ; Neurons ; transplantation ; Spinal Cord ; transplantation ; Spinal Cord Injuries ; surgery ; Tissue Transplantation ; methods

Comet Assay ; DNA Damage ; DNA Repair ; In Situ Hybridization, Fluorescence ; methods

Calcium Pyrophosphate ; metabolism ; Cartilage, Articular ; metabolism ; pathology ; Chondrocalcinosis ; diagnostic imaging ; metabolism ; pathology ; surgery ; Diagnosis, Differential ; Female ; Gout ; pathology ; Humans ; Knee Joint ; diagnostic imaging ; metabolism ; pathology ; Male ; Menisci, Tibial ; metabolism ; pathology ; Middle Aged ; Osteoarthritis ; etiology ; pathology ; Radiography

Animals ; Arthritis ; diagnosis ; pathology ; Arthritis, Rheumatoid ; diagnosis ; pathology ; Humans

[Objective]A new limited contact femoral supracondylar interlocking intramedullary nail(LCFSIIN) was made,which was part of a new custom-made hemi-knee joint,to improve the results of childrens' limb salvage.[Method]A new LCFSIIN was performed and the stress of bolts,intramedullary nails and bone were analyzed by finite dement analysis(FEA) while the bolts were implanted in different angulafions-0?,15?,30?,45?and 60?.[Result]The results of FEA show that the stress of bolts,bone and nails with 15? insertion angles of bolt was the tiniest and well distributed.Stress of bone with 0? insertion angles was more obvious than that with 15?,30?and 45?.The more the angles,the stress of proximal bolt became more obvious and the stress of distal bolt decreasing.[Conclusion]This new LCFSIIN can reduce destruction of biological environment of bone and is easy in use in limb salvage surgery of allograft.