Nanostructures ; adverse effects ; toxicity ; Nanotechnology ; Safety

The quality of life(QOL) in patients with nasopharyngeal carcinoma ( NPC ) has attracted people' s attention increasingly. Most of the studies focus on measuring scale and influential factor. Both the disease itself and many non-somatic factors can affect the patients' quality of life. In this review, we summarize these research advancements on measuring scales and influential factors, considering that it need more studies regard to quality of life in NPC patients, and it is very urgent and important to work out the special measuring scale of NPCQOL that suits Chinese culture and value system.

Objective To investigate the association between MPO gene single nucleotide polymorphisms (SNP) loci (rs2333227,-643G/A) and clinical characteristics in Kawasaki disease (KD) in Han population in central China. Methods A case-control study was performed. Two hundred and thirty-seven children with KD and 249 normal children were recruited. The polymorphism distribution of SNP was detected using PCR-RFLP. The clinical data of children with KD were collected. Results The frequency of SNP loci (rs2333227) genotypes (GG, GA, AA) was signiifcantly different between children with KD and normal children (P=0.039), the allele frequency was also signiifcantly different between two groups (P=0.012), and the G allele was the risk factor. Compared with other genotypes, KD children with GG genotype had higher frequency in hand-foot edema (P=0.029). The SNP polymorphism was also associated with peritoneal effusion (P=0.028), however this SNP polymorphism was not associated with conjunctival hyperemia, oral mucosa lesions, and coronary artery lesion (P>0.05), also was not associated to imaging characteristics of hepatomegaly, splenomegaly, and lobular pneumonia (P>0.05). Conclusion The SNP loci (rs2333227) in MPO gene was associated with KD susceptibility, the G allele was a risk factor, and the SNP polymorphisms is associated with some clinical characteristic.

Objective To explore the contamination by Legionella pneumophila in cooling water of centralized air condi-tioning system in hospitals and hotels.Methods In Aug2001,the water from cooling towers of centralized air conditioning sys-tems in4hospitals and2hotels were sampled in W city.The membranes filtering the cooling water samples were treated by hy-drochloric acid.After treatment,the mixed treated solution was inoculated into GVPC plate and cultured in candle jar.The susepectable colonies were identified by biochemical test,serological test and PCR test respectively.Results5strains of L pneu mophila in cluding3strains of Lp1type and2strains of Lp5type were isolated from water samples of the cooling towers of centralized air conditioning systems in2hospitals and one star-grade hotel in W city.Conclusion The contamination by le-gionella in water of cooling tower of centralized air conditioning system in hospitals and hotels should be monitored closely.

Objective To explore the clinical features of Marfan syndrome (MFS) and its virulence gene mutation of FBN1.Methods Clinical data of 2 children with MFS were retrospectively analyzed, and pertinent literatures were reviewed. Results Case one was a 1 year and 10 months old boy with a special face, bilateral lower eyelid edema, high palatal arch, slender fingers and toes. A little of moist rales in lung could be heard, and systolic accentuated in apex could be heard too. Echocardiography showed that aortic coronary sinus dilated, aorta and pulmonary artery broadened, left ventricular diverticulum, a small amount of mitral regurgitation,and moderate tricuspid regurgitation. Electrocardiogram showed incomplete right bundle branch block. Gene detection found a c.3037G>A mutation (p.Gly1013Arg) inFBN1. Case two was a 12 years old slender boy with spider-like ifnger/toe, high myopia, 2/6 systolic and diastolic murmur in the ifrst and two auscultation area in aortic valves. Echocardiography showed the aortic sinus signiifcantly broadened, aortic incompetence, mild pulmonary regurgitation and left ventricular enlargement. Gene detection found heterozygous mutation of c.1876G>A (p.Gly626Arg) in FBN1, which has not been reported.Conclusion The diagnosis of MFS can be conifrmed byFBN1 gene detection. A new mutation of c.1876G>A (p.Gly626Arg) was detected.

Objective To investigate how SDF-1α and c-MYC protein regulates TACRl-Tr expression. Methods c-myc shRNA vector was constructed, small interfering RNA was employed for silencing c-myc gene in MCF-7 breast cancer cell. SDF-1α neutralized antibody was used in c-myc+ cell group and c-myc- cell group, while other c-myc+ cell group and c-myc- cells group were cultured under normal condition. The mRNA level of TACRl-Tr was determined by real-time PCR. Results c-myc shRNA vector was constructed successfully, in the normal presence of SDF-la, the level of TACRl-Tr mRNA in c-myc- cell group were lower than that in c-myc+ cell group( P < 0.05). But in the presence of SDF-la neutralized antibody, TACRl-Tr mRNA level of c-myc- cell group was higher than that of c-myc+ cell group(P < 0.05). Conclusion In the normal culture condition, c-MYC protein may transactivate TACRl-Tr transcription in MCF-7 cell, in the presence of SDF-1α neutral antibody, c-MYC protein lost the activity of transactivating for TACRl-Tr transciption.

Brain Injuries ; Erythropoietin ; Humans ; Infant, Newborn

Female ; Humans ; Infant, Newborn ; Male ; Serologic Tests ; Syphilis, Congenital ; diagnosis ; drug therapy

Biomarkers ; analysis ; Genomics ; methods ; trends ; Humans ; Preventive Medicine ; methods ; trends

DNA Methylation ; Humans ; Poly(ADP-ribose) Polymerases ; genetics ; metabolism