Objective: To discuss the angioarchitectual characteristics of dural arteriovenous fistulas (DAVFs) and its appropriate therapeutic strategies. Methods: A total of 159 patients with DAVFs were retrospectively analyzed, including the clinical presentations, patterns of angioarchitecture. The appropriate therapeutic strategies, and the factors affecting the efficacy of treatmen were analyzed. Results: The patients with DAVFs were treated with different approaches. Sixty-nine patients with their DAVFs were treated in the cavernous sinus area, including type I (n = 58) and type II (n = 11); 41 patients in the lateral sinus area, including type I (n = 26), type II (n = 14) and type III (n = 1); 13 patients in the superior sagittal sinus area, including type I (n = 1), type II (n = 2) and type III (n = 10); 20 in the tentorial area, and 7 in the skull base area were all type III. There were 9 patients with multiple DAVFs. The main therapeutic approaches used were endovascular treatment. Postoperative angiography demonstrated that 79 patients were cured, 71 improved, and 9 failed. Postoperative complications were found in 9 patients, among them 8 were cured or improved and 1 died. Conclusion: The clinical presentation, treatment and prognosis depend upon the angioarchitecture, the location and the venous drainage patterns. Endovascular treatment is the first choice and chief therapeutic approach.

Objective To screen the conservative,stable and specific DNA signature sequence in the plasmid of Yersinia pestis.Methods Specific validation trials and stability of the qualification test were carried out to 40 strains of Yersinia pestis,47 strains of non-Yersinia pestis of home and wild types of rodent in Yunnan,by using 32 DNA sequences derived from Yersinia pestis in the plasmid and conventional PCR technology,and Yersinia pestis vaccine strain EV76 as a positive control.Results Four pairs of relatively conservative,stable and specific DNA marker genes were screened:YPMT1.05c,YPMT1.03c,YPMT1.42 and YPMT1.04c.Conclusions The 4 pairs of Yersinia pestis DNA signature sequences can be used for rapid diagnosis of plague.

Humans ; Musculoskeletal Diseases ; psychology ; Occupational Diseases ; psychology ; Psychology, Social

Angiogenesis Inhibitors ; pharmacology ; Animals ; Drugs, Chinese Herbal ; pharmacology ; Humans ; Neovascularization, Pathologic ; drug therapy ; Phytotherapy

Objective To design a fast method based on wavelet analysis for fMRI data. Methods Lifting wavelet decomposition instead of stationary wavelet decomposition was utilized to separate paradigm responsive signal and confound ones in fMRI data, while frequency analysis was used to find out the wavelet scales in which paradigm responsive signal existed, then reconstructed signal from these scales was subjected to correlation analysis for actived pixels. Results Analyzing visual fMRI data revealed that when the significant level was α＜10-6, the proposed method gave more sensitive results than correlation analysis, but process time decreased on a large scale compared with the one based on the stationary wavelet transform. At the mean time, the proposed method only used 24 timepoints of data for wavelet reconstruction while one based on stationary wavelet transform used 256 timepoints of data. Conclusion The proposed method is the fast one based on wavelet transform for analyzing fMRI data, which also gives an effective technique for compressing fMRI data.

Objective To discuss the feasibility,indications,surgical techniques,and outcome con- cerning the application of less invasive stabilization system (LISS) for complex proximal femoral fractures.Methods We treated 12 complex proximal femoral fractures using femoral LISS reversely from June 2005 to May 2006. They were five complex intertrochanteric fractures and seven subtrochanteric ones.By AO classification,two were type 31-A2.2,two type 31-A2.3,one type 31-A3.3,one type 32-A3.1,three type 32-B1.1,one type 32-B2.1, and two type 32-B3.I.The patients were treated with closed or indirect reduction and fixation with percutaneous LISS plate reversely.Results There were no major postoperative complications in this study.The mean operation time was 65 minutes (range,50 to 90 minutes),the mean intra-operative blood loss was 142 milliliters (range,50 to 400 milliliters),and the mean postoperative hospital stay was 9.3 days (range,6 to 15 days).All patients had a clinical follow-up;the mean follow-up time was 7.2 months (range,3 to 14 months).Ten cases healed clinically three months postoperatively,one periprosthetic fracture healed four months and one pathological fracture healed six months after operation.At the final radiographic follow-up,no collapse,varus deformity,cutting-out,hardware failure,or avascular necrosis was found.Conclusions Femoral LISS used reversely can provide secure fixation for proximal femoral fractures biomechanically and anatomically.It is also easy and safe.It is particularly suitable for old patients with intertrochanteric fractures complicated with disorders of internal organs and severe osteoporosis or patients with complex proximal femoral fractures.It is important to be skillful in indirect reduction and positioning of guide wire into hole A,and to avoid immediate weight-bearing postoperatively.

Objective To observe the intluence ot chemokine RANTES influence on cardiac allograft acute rejection caused by alloreactive memory CD4+ T cells (Tm) adoptive transfer.Methods Heterotopic heart transplantation (HTx) from Balb/c donors to C57BL/6 recipients was performed by anastomosis of the vessels of the neck.Mice undergoing heterotopic heart transplantation received either adoptive transfer of 1 × 106 CD4+ Tm from the spleen of alloantigen-primed C57BL/6 mice or no cells (control group).After the cardiac transplantation,the mean survival time (MST),mean histologic rank of rejection,relative gene expression and serum concentration of RANTES in the cardiac grafts.Results (1) The percentage of CD4+ Tm was 26.83％ at the spleen of alloantigenprimed mice; (2) The MST was 5.17 ± 0.17 days in the CD4+ Tm+ HTx group versus 7.76 ± 0.21 days at the HTx group (control group) (P＜0.01); (3) The histological tests revealed that mean histologic rank of rejection activity in the sections of cardiac allografts on the day 5 post grafting was grade 3.92 ± 0.08 in the HTx+ CD4+Tm group versus grade 2.67 ± 0.14 in HTx group (P＜0.01) ;(4) The relative gene expression level of RANTES was 2.6 ± 0.21 in the CD4+ Tm + HTx group,significantly higher than in the control group (P＜0.01) ; (5) The serum concentration of RANTES in the CD4+ Tm+ HTx group was 223.6 ± 16.79 pg/mL,higher than in the control group (120.7 ±9.47 pg/mL,P＜0.01).Conclusion Alloreactive CD4+ Tm contribute to the increased expression and secretion of RANTES,and cardiac allograft acute rejection was more extensive in the CD4 + Tm + HTx group.

On the basis of the development of a new CE method for the analysis of α-lactalbumin (α-Lac), β-lactoglobulin A(β-LgA) and β-lactoglobulin B(β-LgB), different concentrations of acetic acid (HAc) and trichloroacetic acid (TCA) for the removal of proteins in raw milk, infant formula A and B, and yogurt were investigated.It was shown that TCA is better than HAc for eliminating of proteins in the above four samples.The proteins in 2 g of raw milk could be eliminated by 3 mL of 100 g/L TCA.0.5 g of infant formula A and B needs 5 mL of 10 g/L and 20 g/L TCA to remove the proteins, respectively.For 2 g of yogurt, 3 mL of 20 g/L TCA is enough.The present research may provide valuable information for protein-containing sample (such as raw milk, infant formula and yogurt) pretreatment.

Background and purpose: The role of lymphadenectomy for endometrial cancer is still controversial. Few gynecologists in China carry out pelvic and/or para-aortic lymphadenectomy for patients with endometrial cancer. The aim of the current study was to investigate the role of systematic lymphadenectomy in the treatment strategy of endometrial cancer and the feasibility in carrying out the surgery. Methods: We performed a retrospective chart review of all patients with endometrial cancer who experienced surgical staging at Cancer Hospital, Fudan University from January 2005 to July 2008. Lymph nodes metastatic status, alteration of adjuvant therapy and surgical complications were discussed. Results: Nineteen (14.8%) of 128 patients undergoing systematic lymphadanectomy had lymph node metastases: both pelvic and para-aortic in 7 patients, only pelvic in 8 patients, and exclusively isolated to the para-aortic area in 4 patients. Therefore, more than half of the patients with lymphatic dissemination had para-aortic lymph nodes metastases. Tumor grade, histological type, myometrial invasion and lymph-vascular space invasion were associated with lymph nodes metastases. Adjuvant chemotherapy and/or tumor-directed radiotherapy were needed for 15 patients upstaged due to lymph-nodal invasion (P<0.05). Furthermore, adjuvant therapy was eliminated for 50 intermediate/intermediate-high risk patients with negative lymph nodes and extrauterine spread. Complications were found in 8 patients: 3 pelvic infection, 2 residual vaginal bleeding, and 1 pero-bowel obstruction, 1 deep venous thrombosis accompanied with lymphocyst, and 1 lacunar infarction. The median time of the procedure was 150 minutes, median blood loss was 300 mL, and 27 patients received blood transfusion. Conclusion: The findings of the current study suggest that it is safe and feasible to carry out systematic lymphadenectomy in women with endometrial cancer. Surgical staging can assess the status of lymph nodes, provide accurate prognostic information, and help to formulate adjuvant therapy after surgery.

Objective The aim of this study was to find whether modified radical hysterectomy or radical hysterectomy improves local-regional control for patients with stage Ⅰ endometrioid carcinoma. Methods The medical records of patients with stage Ⅰ endometrioid carcinoma who were enrolled in Cancer Hospital of Fudan University between 1996 to 2008 after undergoing modified radical hysterectomy or radical hysterectomy were reviewed.The Kaplan-Meier method was used for time-to-event analysis with recurrence and death as the end points. Results Among 518 women with stage Ⅰendometrioid carcinoma, 474 underwent modified radical/radical hysterectomy and bilateral salpingo-oophorectomy±pelvic lymphadenectomy±peri-aortic lymphadenectomy+cytology.Twelve patients (2.5%) received neoadjuvant chemotherapy or vaginal brachytherapy before operation, and 73 patients (15.4%) received postoperative adjuvant therapy (pelvic external beam radiotherapy or chemotherapy or combination).After a median follow-up of 30 months,16 relapses were observed.Eight patients suffered with distant metastases, 4 with vaginal recurrences and 4 with pelvic recurrences.The 3-year and 5-year cumulative vaginal recurrence rates were 1.4% and 2.0%. The 3-and 5-year cumulative local-ragional recurrence rates were 2.5% and 3.1%. The 3-and 5-year actuarial overall survival rates were both 98.1%.The 5-year local-regional recurrence rates for stage Ⅰa, stage Ⅰb, stage Ⅰc were 3%, 3.7% and 0 (P=0.649), and the 5-year survival rates were 98.3%, 97.8% and 100% (P=0.399). There was no evidence of benefit in terms of lympnadenectomy in patients with stage Ⅰ endometrioid carcinoma(P value were 0.525 and 0.665,respectively).The median operating time was 135 minutes, the median blood loss was 300 mL, and 15.4% of the patients needed blood transfusion. Surgery associated morbidity was 7%, and no one died of surgery associated morbidity. Conclusions Modified radical hysterectomy or radical hysterectomy is a viable and possibly preferable option for patients with stage Ⅰ endometrioid carcinoma.Randomized clinical trials were urgently needed to address the utility of modified radical hysterectomy in stage Ⅰ endometrial cancer.