Objective: To discuss the angioarchitectual characteristics of dural arteriovenous fistulas (DAVFs) and its appropriate therapeutic strategies. Methods: A total of 159 patients with DAVFs were retrospectively analyzed, including the clinical presentations, patterns of angioarchitecture. The appropriate therapeutic strategies, and the factors affecting the efficacy of treatmen were analyzed. Results: The patients with DAVFs were treated with different approaches. Sixty-nine patients with their DAVFs were treated in the cavernous sinus area, including type I (n = 58) and type II (n = 11); 41 patients in the lateral sinus area, including type I (n = 26), type II (n = 14) and type III (n = 1); 13 patients in the superior sagittal sinus area, including type I (n = 1), type II (n = 2) and type III (n = 10); 20 in the tentorial area, and 7 in the skull base area were all type III. There were 9 patients with multiple DAVFs. The main therapeutic approaches used were endovascular treatment. Postoperative angiography demonstrated that 79 patients were cured, 71 improved, and 9 failed. Postoperative complications were found in 9 patients, among them 8 were cured or improved and 1 died. Conclusion: The clinical presentation, treatment and prognosis depend upon the angioarchitecture, the location and the venous drainage patterns. Endovascular treatment is the first choice and chief therapeutic approach.

Objective To screen the conservative,stable and specific DNA signature sequence in the plasmid of Yersinia pestis.Methods Specific validation trials and stability of the qualification test were carried out to 40 strains of Yersinia pestis,47 strains of non-Yersinia pestis of home and wild types of rodent in Yunnan,by using 32 DNA sequences derived from Yersinia pestis in the plasmid and conventional PCR technology,and Yersinia pestis vaccine strain EV76 as a positive control.Results Four pairs of relatively conservative,stable and specific DNA marker genes were screened:YPMT1.05c,YPMT1.03c,YPMT1.42 and YPMT1.04c.Conclusions The 4 pairs of Yersinia pestis DNA signature sequences can be used for rapid diagnosis of plague.

Objective To design a fast method based on wavelet analysis for fMRI data. Methods Lifting wavelet decomposition instead of stationary wavelet decomposition was utilized to separate paradigm responsive signal and confound ones in fMRI data, while frequency analysis was used to find out the wavelet scales in which paradigm responsive signal existed, then reconstructed signal from these scales was subjected to correlation analysis for actived pixels. Results Analyzing visual fMRI data revealed that when the significant level was α＜10-6, the proposed method gave more sensitive results than correlation analysis, but process time decreased on a large scale compared with the one based on the stationary wavelet transform. At the mean time, the proposed method only used 24 timepoints of data for wavelet reconstruction while one based on stationary wavelet transform used 256 timepoints of data. Conclusion The proposed method is the fast one based on wavelet transform for analyzing fMRI data, which also gives an effective technique for compressing fMRI data.

Angiogenesis Inhibitors ; pharmacology ; Animals ; Drugs, Chinese Herbal ; pharmacology ; Humans ; Neovascularization, Pathologic ; drug therapy ; Phytotherapy

Humans ; Musculoskeletal Diseases ; psychology ; Occupational Diseases ; psychology ; Psychology, Social

Objective To discuss the feasibility,indications,surgical techniques,and outcome con- cerning the application of less invasive stabilization system (LISS) for complex proximal femoral fractures.Methods We treated 12 complex proximal femoral fractures using femoral LISS reversely from June 2005 to May 2006. They were five complex intertrochanteric fractures and seven subtrochanteric ones.By AO classification,two were type 31-A2.2,two type 31-A2.3,one type 31-A3.3,one type 32-A3.1,three type 32-B1.1,one type 32-B2.1, and two type 32-B3.I.The patients were treated with closed or indirect reduction and fixation with percutaneous LISS plate reversely.Results There were no major postoperative complications in this study.The mean operation time was 65 minutes (range,50 to 90 minutes),the mean intra-operative blood loss was 142 milliliters (range,50 to 400 milliliters),and the mean postoperative hospital stay was 9.3 days (range,6 to 15 days).All patients had a clinical follow-up;the mean follow-up time was 7.2 months (range,3 to 14 months).Ten cases healed clinically three months postoperatively,one periprosthetic fracture healed four months and one pathological fracture healed six months after operation.At the final radiographic follow-up,no collapse,varus deformity,cutting-out,hardware failure,or avascular necrosis was found.Conclusions Femoral LISS used reversely can provide secure fixation for proximal femoral fractures biomechanically and anatomically.It is also easy and safe.It is particularly suitable for old patients with intertrochanteric fractures complicated with disorders of internal organs and severe osteoporosis or patients with complex proximal femoral fractures.It is important to be skillful in indirect reduction and positioning of guide wire into hole A,and to avoid immediate weight-bearing postoperatively.

High mobility group box-1 protein (HMGB1) has recently been shown as a crucial late mediator of inflammation and sepsis, and is involved in mediating multi-organ functional lesions, including acute lung, liver, and intestine injuries. As a delayed inflammatory cytokine, HMGB1 provides a wider therapeutic time window for clinical intervention. HMGB1 has been proven to be a promising therapeutic target to prevent the development of multiple organ dysfunction syndrome in experimental models of severe sepsis. The pharmacological strategies include neutralization of antibodies or specific HMGB1 antagonists, suppression of HMGB1 secretion (ethyl pyruvate, agonists for alpha7-nicotinic acetylcholine receptors), and down-regulation of HMGB1 expression (sodium butyrate, signaling inhibitors for Janus kinase/signal transducer and activator of transcription).
HMGB1 Protein ; antagonists & inhibitors ; biosynthesis ; physiology ; Humans ; Multiple Organ Failure ; immunology ; metabolism ; prevention & control ; Sepsis ; immunology ; metabolism ; therapy

Animals ; Blood Glucose ; metabolism ; Body Weight ; Diet ; Female ; Lipids ; blood ; Male ; Oxidation-Reduction ; Rats ; Rats, Wistar ; Temperature

Animals ; Female ; Gonads ; physiopathology ; Hypothermia ; chemically induced ; physiopathology ; Male ; Rats ; Rats, Wistar ; Sex Factors ; Soman ; adverse effects ; pharmacology

Objective The absorbable haemostatic membrane was used to stop bleeding during posterior spinal surgery so as to investigate the therapeutic effect and the security of the absorbable haemostatic membrane. Methods Ninety-four patients were enrolled into the experimental group and the control group. In the experimental group , the decompression wound is handled by using the regular absorbable haemostatic membrane to stop bleeding. In the control group , the conventional surgical methods , such as coagulation , brain cotton and other methods were used to stop bleeding. After the operations , patients in two groups were given indwelling vacuum drainage tube. The post operation vacuum pipe drainage , the drainage tube removing time , the incidence of postoperative complications after removing drainage tubes , the reoperation number , vital signs after 24 hour (temperature, respiration, pulse, blood pressure), and laboratory tests (blood count, coagulation function parameters) were determined and compared between two groups. Results Both the vacuum drainage at 24 hours post operation and the total vacuum drainage post operation were significantly reduced in the experimental group(P < 0.05, respectively). No significant differences in the coagulation function parameters were found between both two groups. Conclusion The absorbable haemostatic membrane may be applicable for spinal surgery.