AIM: To investigate the antagonistic action of Captopril (Cap) on the activation and injury of human umbilical vascular endothelial cells (HUVECs) induced by lipopolysaccharide(LPS) and the possible mechanisms. METHODS: After 18 h exposure of the cultured HUVECs to LPS (1 mg/L), or LPS (1 mg/L) plus Cap at the concentration of 10~(-7)mol/L, 10~(-5)mol/L and 10~(-3)mol/L, the expression of vWF protein in the conditioned media was tested by enzyme-linked immunosorbent assay (ELISA), the expression of ICAM-1 protein in HUVECs was determined by indirect immunofluorescence technique with flow cytometry as well. In addition, the expression of TNF? mRNA was determined by in situ hybridization. RESULTS: The results of ELISA and indirect immunofluorescence technique showed that exposure to LPS at a concentration of 1 mg/L led to a significant increase in the vWF and ICAM-1 expression in HUVECs as compared to the control (P

Toxicogenomics is a new undertaking in the pursuit of human genomics relevant to health risk from environmental toxicants and related stress, and also is a new scientific subdiscipline derived from a combination of the fields of toxicology and genomics. Microarray technique, i. e. gene-chip technique includes gene-chip development, pretreatment of detected samples, probe hybridization, detection data analysis. The microarray technique in toxicology mainly was applied to identification of potential hazardous substances, screening for mechanism of action, dose-response relationship assessment, identification of interaction among chemical mixtures, biomarker of exposure and biomarker of susceptibility. Microarray technique has been used to study biomarker for human bladder cancer, to study fingerprints of gene regulation associated with cadmium chloride, benzo(a) pyrene and trichloroethylene. fn this paper, a new automated and multi-applied molecular biology workstation, NanoChip biomolecular system was introduced .

Fecal microbiota transplantation (FMT) has become a key treatment for Clostridium difficile infection (CDI).The Chinese fecal microbiota bank rescue plan is a non-profit central platform for the treatment of refractory CDI using FMT.This article reviewed and analyzed the principle,protocol and risk management of the Chinese fecal microbiota bank rescue plan.

China ; Cone-Beam Computed Tomography ; Dental Stress Analysis ; Humans ; Imaging, Three-Dimensional ; Malocclusion ; diagnosis ; etiology ; therapy ; Orthodontics ; manpower ; trends ; Orthodontics, Corrective ; methods

Personnel resource is very important for the development of emergency department and emergency medicine.In recent years a series of researches for the standardized training of emergency residents have been progressed in the Emergency Depart- ment of West China Hospital.It goes through three phases.The idea of the training changes from“general physician”to“emer- gency specialist”.And the training is designed,practised and assessed with standardization.

Orthodontic Anchorage Procedures

Objective: To investigate the relationship between the expressions of the transforming growth factor beta 1 (TGF-β1), Smad4, and TβR II in the different stages of gallbladder carcinoma and discuss their roles in the development of gallbladder carcinoma. Methods: Immunohistochemical SP method was used to examine the expression of TGF-β1, Smad4, and TβR II in 30 cases of gallbladder carcinoma, 11 cases of gallbladder adenoma, and 30 cases of cholecystitis. The relationship between the expressions of TGF-β1, Smad4 and TβR II and the clinicopathological features of gallbladder carcinoma were analyzed. Results: The positive rates of TGF-β1, Smad4 and TβR II was 73.3%, 20%, and 16.7% in 30 cases of gallbladder carcinoma; 90.9%, 63.7%, and 54.5% in 11 cases of gallbladder adenoma; 96.7%, 93.3%, and 90% in 30 cases of cholecystitis. The expression of TGF-β1 was significantly lower in gallbladder carcinoma than that in cholecystitis (P<0.05). The expressions of Smad4 and TPR H were significantly lower in gallbladder carcinoma than that in cholecystitis (P<0.05). Gallbladder carcinoma at stages I and II had lower TGF-β1 expression but higher Smad4 and TβR II expression than those at stage III and V (P<0.05). TGF-β1 expression was significantly higher (94.1%) in gallbladder carcinoma with metastasis than that without metastasis (P<0.05). Conclusion: The decreased expression of TGF-β1 in gallbladder carcinoma may be related with the cell malignant transformation and uncontrolled growth. Over-expression of TGF-β could not inhibit the proliferation of gallbladder carcinoma which may be due to the decreased expression of Smad4 and TβR II. The high expression of TGF-β1 is related with the progression, invasion, and metastasis of gallbladder carcinoma.

Animals ; Gene Silencing ; physiology ; Gene Targeting ; Genetic Therapy ; methods ; Genomics ; Humans ; RNA Interference ; RNA, Small Interfering ; genetics ; therapeutic use ; RNA-Induced Silencing Complex

Objective To explore the effects of Stilnox on the blood pressure and quality of life(QOL)in pa- tients with hypertension and insomnia.Methods 80 patients with hypertension and insomnia were received am- lodipine(5mg qd)as control group and amlodipine(5mg qd)plus stilnox(10mg qn)as treatment group in visit order for 8 weeks'follow up.Values of blood pressure were recorded and QOL data were assessed at the beginning and end of therapy with questionnaire.Results Blood pressure was significantly reduced in both groups(P