Dyslipidemia plays an important role in the pathogenesis of diabetic retinopathy (DR).A preliminary study found that low-density lipoprotein cholesterol,apolipoprotein (Apo) B and Apo B / Apo A1 ratio were positively correlated with DR,while high-density lipoprotein cholesterol,Apo A1 was negatively correlated with DR and proliferative DR.Reducing the blood fats to be helpful to DR control.However,the mechanism of hyperlipidemia in the pathogenesis of DR,the reason of dyslipidemia in diabetic patients and the interaction between hyperglycemia and hyperlipidemia in DR are not clear yet.Moreover,there is no predictive indicators related to blood lipid for DR.Understanding the relationship between dyslipidemia and DR can provide definite evidence for fat-reducing therapy for DR control.

AIM:To investigate the corneal histopathology changes induced by soft contact lenses ( SCL) at diffent time.
METHODS: Central and peripheral cornea structures of myopic patients wearing SCL (150 cases) and the control goups ( 150 cases ) were observed with confocal microscopy. The examinations were performed 1, 3, 5, 8 and 10a after contact lenses wearing respectively.
RESULTS: After wearing the contact lenses for 5a, the cell density of corneal surface epithelial was lower in SCL wearers compared with control goups (P<0. 05) and the corneal vesicle and corneal gutata might be occasionally discovered. The density of Langerhans cells both in the central and peripheral cornea was higher in SCL group ( P<0. 05 ), and the tortuosity of the nerve fibers was significantly increased in the eye wearing SCL compared with control group. The densities of anterior stromal cells were lower while the microdot densities in the stroma were higher in SCL group than that in control group ( P<0.05 ) . The polymorphology of corneal endothelial cells was more obvious in SCL group, but the densities of endothelial cells were similar between two groups ( P>0.05). The SCL wearers showed a thinner thickness of central corneal epithelium than control group (P<0. 05).CONCLUSION: The corneal morphologic changes are more obvious with the longer time, and the changes is not ignored.

Humans ; Photography ; methods ; Surgery, Oral

Humans ; Photography ; methods ; Surgery, Oral

Gas Chromatography-Mass Spectrometry ; methods ; Humans ; Malathion ; metabolism ; urine

Objective To explore the methods of extraction, isolation, purification, and biological activities of ray cartilage glycosaminoglycans (RCG). Methods RCG was purified by guanidine hydrochlorid extraction, acetone fractional precipitation, ultrafiltration, and Sephadex column chromatography. The purity and molecular mass of RCG were measured by means of HPLC. The model of mouse with Lewis lung carcinoma was made, the experimental mice were randomly divided into normal saline group, RCG (500, 250, and 125 mg/kg) groups, and CTX (60 mg/kg) group. Tumor growth states of mice were observed, tumor growth curve was described, inhibitory rates of primary tumor and number of lung metastasis focus were measured; microvessel density (MVD) was quantitated by immunohistochemistry using monoclonal antibodies of CD31; the expression of vascular endothelial growth factor (VEGF) mRNA was determined with RT-PCR. Results Using HPLC, a single glycosaminoglycans with molecular mass 9.7?104 was collected and its purity exceeded ninty-nine percent. Tumor growth curves in RCG groups were smooth compared with saline group. There were significant differences of inhibitory rates of primary tumor, number of lung metastasis focus and MVD between RCG groups and saline group. VEGF mRNA expression levels in RCG groups were reduced significantly compared with saline group. Conclusion RCG could effectively inhibit the growth and metastasis of primary Lewis lung carcinoma in C57BL/6 mouse and angiogenesis.

Air Pollutants, Occupational ; analysis ; Naphthols ; analysis ; Spectrophotometry ; methods ; Workplace

Humans ; Laryngeal Neoplasms ; surgery ; Larynx ; surgery ; Laser Therapy ; Microsurgery

Humans ; Rhinitis, Allergic, Perennial ; Rhinitis, Allergic, Seasonal

Objective: To investigate the relationship of chemokine receptor with the development and progression of primary biliary cirrhosis (PBC). Methods: Real-time PCR and flow cytometry were used to examine the mRNA and protein expression of chemokine receptor 1 (CCR1), CCR3 and CCR5 in the peripheral blood mononuclear cells (PBMCs) of 60 patients with PBC, 60 patients with hepatitis B-related cirrhosis, and 60 normal controls. Total bilirubin (TBIL) and γ-glutamyltransferase (γ-GT) levels were determined in the patients with PBC and normal controls,and their correlation with chemotactic factors was also analyzed. Results: Both the mRNA and protein expression levels of CCR1, CCR3 and CCR5 in the PBMCs were significantly lower in PBC patients than those in the other two groups (P< 0.05), and there were no significant differences between the latter two groups. The mRNA and protein levels of CCR1, CCR3 and CCR5 were significantly lower in patients with stage III and IV PBC than those with stage I and II PBC. CCR1 protein expression was correlated with TBIL levels in PBC patients(r= - 0.445,P<0.01), but not with γ-GT(r= - 0.230,P>0.05). CCR3 protein was not linearly correlated with TBIL level (r= -0.173,P>0.05),but was correlated with γ-GT(r= -0.295, P< 0.05). Expression of CCR5 protein was negatively correlated with both TBIL and γ-GT levels(r= -0.531,P<0.01; r=-0.665,P <0.01). Conclusion: CCR1, CCR3 and CCR5 expression is associated with the development and progression of PBC; they may be involved in the regulatory mechanism of PBC,which may cast new lights on the diagnosis and prevention of PBC.